Journal of cell science & therapy最新文献

{"title":"Personalized Nutrition Therapy in Hashimoto's Thyroiditis and Herpes Zoster Oticus, Recalcitrant to Conventional Therapy: A Case Report","authors":"Yang Sw","doi":"10.35248/2157-7013.19.10.288","DOIUrl":"https://doi.org/10.35248/2157-7013.19.10.288","url":null,"abstract":"Personalized nutrition therapy (PNT) has been developing in terms of functional medicine. Its efficacy has been controversial. In the scope of functional medicine, the diagnostic methods to analyze blood are generally based on two kinds of perspectives; biochemistry, blood chemistry and CBC with differential counts. Out of two methods, I devised the latter of my own with imbibing the result of my Pulse Pattern Diagnosis (PPD). The PPD established by me can measure the metabolic, inflammatory, and/or hemodynamic states of major organs, using the radial artery in the wrist of each person. For example, when hepatobiliary tract cells are metabolically activated, and/or the liver is hyper-perfused, my PPD indicates correlation with the increased value of uric acid in blood analysis. The effect of vitamin B5 on liver decreases the metabolic rate and blood flow into the liver in terms of my PPD. It has been reported that vitamin B5 is important in breaking down the excess of uric acid in our body. Likewise, my blood analytic algorithm for PNT is created. I have four clinical records of two kinds of disease entities; herpes zoster oticus with acute otitis media (1 case), thyroid Hashimoto’s thyroiditis (3 cases). These cases were not recalcitrant to conventional therapy but improved after PNT. In summary, PNT may be an alternative to the invincible infectious and autoimmune diseases recalcitrant to conventional therapy.","PeriodicalId":73642,"journal":{"name":"Journal of cell science & therapy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69971910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arrangement of Human Cell Metaphase Chromosomes on the Equatorial Plate","authors":"T. Jiang, Chunxiao Wu, A. Wajid, Donyun Jiang, Han Huan, Zhiren Zhan, Jiacheng Huang","doi":"10.35248/2157-7013.19.10.289","DOIUrl":"https://doi.org/10.35248/2157-7013.19.10.289","url":null,"abstract":"Chromosome is an important hereditary material that maintains the genetic stability of an organism. The structure and location of different chromosomes in the cell governs the basis, how it expresses the genetic effects in the cell and thereby in the whole organism. The chromosome territories (CTs) suggest the location of the chromosomes within the cell by analyzing the karyotypes of human cells. We assume the location and arrangement of chromosomes in the cells is by the interrelationship between chromosomes. More than 100 years ago a Cytologist named Theodor, proposed the idea of chromosome territories. It is indicated that chromosomes are not randomly distributed in Metaphase. This hypothesis is still not directly supported by any laboratory work. We have found in our clinical research that there is a high degree of karyotype expression, so we took a supportive position on this hypothesis but, in order to find the existence of such interrelationships between chromosomes, we conducted a statistical analysis of a large number of existing karyotypic data. In analyzed data we found a clear dominance of long arm over short arm of chromosome according to its activity, with exceptions in specific chromosomes. From the chromosomes statistical data that karyotype occurring between the short arms is relatively small then that of long arm. From the present data it was clear that the exchange of genetic material between the long-long arm is > than exchange of genetic material among long-short arm and short-short arm of the chromosome. Based on the frequency of chromosomal material exchange patterns for arrangement can be considered as circular arrangement, or honeycomb arrangement.","PeriodicalId":73642,"journal":{"name":"Journal of cell science & therapy","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69972132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesangiogenic progenitor cells (MPCs) in orthopedics, a new tool for cell-based medicinal products?","authors":"S. Pacini","doi":"10.4172/2157-7013-c2-051","DOIUrl":"https://doi.org/10.4172/2157-7013-c2-051","url":null,"abstract":"Mesangiogenic progenitor cells (MPCs) have been firstly described in 2008 in human bone marrow (hBM) mononuclear cell cultures, intended to isolate mesenchymal stromal cells (MSCs) in animal-free conditions. Later, we developed a clinical-grade and selective culture method to isolate MPCs with high grade of purity with yields around 1% of plated cells. MPC are characterized by lack of MSC markers, specific integrin profile and phenotype that include CD31 and surprisingly CD45. From the first report on MPCs, these cells showed both mesengenic and angiogenic potential in vitro. Interestingly, and nestin expression were also detected. Mesengenic differentiation protocol has been set up in chemical defined conditions and more recently, the angiogenic potential was clearly demonstrated also in vivo, applying MPC constructs on chicken chorioallantoic membrane. Surprisingly, the ex vivo precursor of MPCs in hBM has been identified in CD45dimCD64brightCD31brightCD14neg population with a morphology resembling the monoblast. For their peculiar differentiation properties and clinical-grade isolating methods, MPCs could represent a new tool for the implementation of cell-based medicinal products (CBMPs) applicable for skeletal tissue regeneration, as these cells could also support the neo-vascularization. In fact, future studies on tissue reconstruction should take in consideration that the newly formed tissue growth and integration should be supported by concomitant neo-vessels formation. The co-existence of mesengenic and angiogenic potential in MPCs could significantly improve the regeneration potential of new therapeutic approaches that involve these interesting cells.","PeriodicalId":73642,"journal":{"name":"Journal of cell science & therapy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70344135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Accuracy and Predictive Value of Latex Agglutination Test, Rapid Cassette Test Compared to ELISA in Diagnosis of Toxoplasma gondii in Pregnant Sudanese Women","authors":"Mohammed Wm, Abdalla Hs, Satti Ab, Kabbashi As","doi":"10.35248/2157-7013.19.10.290","DOIUrl":"https://doi.org/10.35248/2157-7013.19.10.290","url":null,"abstract":"Background: There are different procedures for the diagnosis of the pregnant female suspected with toxoplasmosis, however time, cost, and accuracy of the test should meat patients need. Material and Method: Three hundered pregnant female collected from Saad Abualila hospital antenatal care unit, were undergo three different procedures for the diagnosis of Toxoplasma gondii infection. Toxolatex. Toxo IgG-IgM rapid test and ELISA were done for all pregnant female. The result described as frequency and percentage of positivity, also specificity and sensitivity of Toxolatex. Toxo IgG-IgM rapid test were assessed according to ELISA results. Results: The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and diagnostic accuracy for Latex agglutination test to detect T. gondii antibodies were: 44.6%, 71.9%, 30.5%, 82.4% and 66. %, while the specificity, sensitivity, PPV, NPV and diagnostic accuracy for rapid cassette test to detect T. gondii antibodies were: 29.2%, 88.5%, 41.3%, 81.8% and 75.67%, respectively. Conclusion: Toxo IgG-IgM rapid test(casset) considered as good test for diagnosis of toxoplasmosis and mor specific than Toxolatex with also high diagnostic accuracy.","PeriodicalId":73642,"journal":{"name":"Journal of cell science & therapy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69972148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nuclear Magnetic Coupled Fast Radio Burst Induced Upregulation of Hsp70 in Regeneration of Human Chondrocytes - A Sham Control Study","authors":"Pillai Vk, Shingati Muhammed Hashim, M. Nune, P. Gopal","doi":"10.35248/2157-7013.19.10.292","DOIUrl":"https://doi.org/10.35248/2157-7013.19.10.292","url":null,"abstract":"Manipulating the cell’s inherent vulnerabilities to induce intrinsic changes and re-engineer diseased tissues to drive regeneration is a domain creating significant therapeutic impact through translational medicine. Articular cartilage injuries are recurrently experienced due to trauma, mechanical stress and age-related deterioration. Prevailing mend methods have failed to yield reliable or lasting results. Lack of suitable models that mimic the cellular and extra cellular matrix properties of hyaline cartilage has been one of the reasons for this deficiency. We investigated and observed, joint tissue obtained during total knee replacement surgeries from 7 discrete patients presenting with osteoarthritis and the commonly associated symptoms of inflammation and pain. We used modulated “Nuclear Magnetic Coupled Fast Radio Burst or simply Fast Radio Bursts”, a new modality that seems to trigger the cellular signaling required by the articular cartilage tissue, to mend and regenerate by upregulation of Heat Shock Protein 70. Fast Radio Bursts are high energy, short electromagnetic bursts, in which both electric and magnetic components of the electromagnetic signals are “circularly” polarized. Fast Radio Bursts are produced when a radio signal is traveling through a powerful instantaneous magnetic field on its path to the target. In this Sham controlled study, up-regulation of Hsp 70 protein, to establish its role in an in vitro model was designed to expose 2-Dimensional and 3-Dimensional cultures to Fast Radio Bursts, and compared to a Sham Control, under identical conditions but without exposing Sham Control culture to fast radio bursts. The 2D and 3D reconstructed cartilage tissues were then assessed in both groups. Experiments were conducted to characterize the 2D and 3D cultures to confirm total collagen, fibrillar collagen and proteoglycans, additionally; immunofluorescence and cell viability assay was performed to identify specific bio markers like Collagen 1, Collagen II, Aggrecan, Cell surface Adhesion factor, Hsp70 and cell viability. It could be established in this study that 2D cultures grown in newly defined media and exposed to Fast Radio Burst signals, when compared to 2D cultures grown as Sham culture showed more chondrocyte specific markers and viable matrix properties. In 3D cultures grown similarly also showed better deep layer properties compared to the 3D cultures grown in sham culture. Thus modulated Fast Radio Bursts exposure could play a significant role in specific protein up/down regulation in tissue regeneration.","PeriodicalId":73642,"journal":{"name":"Journal of cell science & therapy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69971876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomics Profiling of Pancreatic Cancer and Pancreatitis for Biomarkers Discovery.","authors":"N Sanh,&nbsp;H Fadul,&nbsp;N Hussein,&nbsp;B D Lyn-Cook,&nbsp;G Hammons,&nbsp;X E Ramos-Cardona,&nbsp;K Mohamed,&nbsp;S I Mohammed","doi":"10.4172/2157-7013.1000287","DOIUrl":"https://doi.org/10.4172/2157-7013.1000287","url":null,"abstract":"<p><p>Pancreatic cancer is one of the most aggressive malignancies with an increase in incidence predicted, particularly in African Americans. Pancreatic cancer is considered a silent disease with poor prognosis and a lack of early biomarkers for detection. Proteomics has been applied in many diseases for identifying or discovering biomarkers. It has long been suggested that chronic pancreatitis may be a risk factor for developing pancreatic cancer. This study identified proteins that are altered in expression in pancreatic cancer and pancreatitis compared to normal using proteomic technology. Proteins were extracted from laser captured micro-dissected tissues and separated in 2-DPAGE and imaged. The protein profiles of pancreatic cancer and pancreatitis are similar but differed with the protein profile of normal adjacent tissues. Representative proteins, overexpressed in tumor and pancreatitis but not normal tissues, were excised from gels, subjected to in-gel digestion, and analyzed by MALDI-TOF mass spectrometry. Proteins identified included transferrin, ER-60 protein, proapolipoprotein, tropomyosin 1, alpha 1 actin precursor, ACTB protein, and gamma 2 propeptide, aldehyde dehydrogenase 1A1, pancreatic lipase and annexin A1. Several proteins, which were shown in pancreatic cancer, were also observed in pancreatitis samples. Understanding the role of these specific proteins and their mechanistic action will give insights into their involvement in pancreatic cancers.</p>","PeriodicalId":73642,"journal":{"name":"Journal of cell science & therapy","volume":"9 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7013.1000287","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37193698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Bax Microsatellite Mutations and BaxΔ2 Isoform in Human Buccal Cells.","authors":"Honghong Zhang,&nbsp;Cecilie Tassone,&nbsp;Nora Lin,&nbsp;Adriana Mañas,&nbsp;Yu Zhao,&nbsp;Jialing Xiang","doi":"10.4172/2157-7013.S8-002","DOIUrl":"https://doi.org/10.4172/2157-7013.S8-002","url":null,"abstract":"<p><p>Loss of the pro-apoptotic Bcl-2 family protein Bax occurs in ~50% of hereditary nonpolyposis colorectal cancer (HNPCC) due to microsatellite instability (MSI). Recently, we found that some of the \"Bax-negative\" MSI tumor cells contain a functional Bax isoform, BaxΔ2, which sensitizes cells to selective chemotherapeutics. Here we show the detection of Bax microsatellite mutations and expression of BaxΔ2 proteins in human buccal cells. Our study provides a sensitive and non-invasive approach and a potential clinical application in diagnosis and treatment of MSI colon cancer patients.</p>","PeriodicalId":73642,"journal":{"name":"Journal of cell science & therapy","volume":"6 Suppl 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7013.S8-002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35710045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Plasticity in Cancer Cells: Reconnecting Mitochondrial Function to Cancer Control.","authors":"V Krishnan Ramanujan","doi":"10.4172/2157-7013.1000211","DOIUrl":"10.4172/2157-7013.1000211","url":null,"abstract":"<p><p>Anomalous increase in glycolytic activity defines one of the key metabolic alterations in cancer cells. A realization of this feature has led to critical advancements in cancer detection techniques such as positron emission tomography (PET) as well as a number of therapeutic avenues targeting the key glycolytic steps within a cancer cell. A normal healthy cell's survival relies on a sensitive balance between the primordial glycolysis and a more regulated mitochondrial bioenergetics. The salient difference between these two bioenergetics pathways is that oxygen availability is an obligatory requirement for mitochondrial pathway while glycolysis can function without oxygen. Early observations that some cancer cells up-regulate glycolytic activity even in the presence of oxygen (aerobic glycolysis) led to a hypothesis that such an altered cancer cell metabolism stems from inherent mitochondrial dysfunction. While a general validity of this hypothesis is still being debated, a number of recent research efforts have yielded clarity on the physiological origins of this aerobic glycolysis phenotype in cancer cells. Building on these recent studies, we present a generalized scheme of cancer cell metabolism and propose a novel hypothesis that might rationalize new avenues of cancer intervention.</p>","PeriodicalId":73642,"journal":{"name":"Journal of cell science & therapy","volume":"6 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34080216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiomyocyte-specific Estrogen Receptor Alpha Increases Angiogenesis, Lymphangiogenesis and Reduces Fibrosis in the Female Mouse Heart Post-Myocardial Infarction.","authors":"Shokoufeh Mahmoodzadeh,&nbsp;Joachim Leber,&nbsp;Xiang Zhang,&nbsp;Frédéric Jaisser,&nbsp;Smail Messaoudi,&nbsp;Ingo Morano,&nbsp;Priscilla A Furth,&nbsp;Elke Dworatzek,&nbsp;Vera Regitz-Zagrosek","doi":"10.4172/2157-7013.1000153","DOIUrl":"https://doi.org/10.4172/2157-7013.1000153","url":null,"abstract":"<p><p>Experimental studies showed that 17β-estradiol (E2) and activated Estrogen Receptors (ER) protect the heart from ischemic injury. However, the underlying molecular mechanisms are not well understood. To investigate the role of ER-alpha (ERα) in cardiomyocytes in the setting of myocardial ischemia, we generated transgenic mice with cardiomyocyte-specific overexpression of ERα (ERα-OE) and subjected them to Myocardial Infarction (MI). At the basal level, female and male ERα-OE mice showed increased Left Ventricular (LV) mass, LV volume and cardiomyocyte length. Two weeks after MI, LV volume was significantly increased and LV wall thickness decreased in female and male WT-mice and male ERα-OE, but not in female ERα-OE mice. ERα-OE enhanced expression of angiogenesis and lymphangiogenesis markers (<i>Vegf, Lyve-</i>1), and neovascularization in the peri-infarct area in both sexes. However, attenuated level of fibrosis and higher phosphorylation of JNK signaling pathway could be detected only in female ERα-OE after MI. In conclusion, our study indicates that ERα protects female mouse cardiomyocytes from the sequelae of ischemia through induction of neovascularization in a paracrine fashion and impaired fibrosis, which together may contribute to the attenuation of cardiac remodelling.</p>","PeriodicalId":73642,"journal":{"name":"Journal of cell science & therapy","volume":"5 1","pages":"153"},"PeriodicalIF":0.0,"publicationDate":"2014-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7013.1000153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32467161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone Marrow Mesenchymal Stem Cells Increase Survival after Ionizing Irradiation Combined with Wound Trauma: Characterization and Therapy.","authors":"Juliann G Kiang, Nikolai V Gorbunov","doi":"10.4172/2157-7013.1000190","DOIUrl":"10.4172/2157-7013.1000190","url":null,"abstract":"<p><p>The aim of this study was to investigate whether treatment with mesenchymal stem cells (MSCs) could improve survival after radiation combined injury. Bone marrow MSCs (BMSCs) were isolated from femurs of B6D2F1/J female mice and were expanded and cultivated in hypoxic conditions (5% O₂, 10% CO₂, 85% N₂) over 30 days. BMSCs were transfused to mice 24 hr after combined injury due to ⁶⁰Co-γ-photon irradiation (9.25 and 9.75 Gy, 0.4 Gy/min, bilateral) followed by skin wounding (CI). Water consumption, body weight, wound healing, and survival tallies were monitored during observation period. Mice subjected to CI experienced a dramatic moribundity over a 30-day observation period. Thus, CI (9.25 Gy)-animal group was characterized by 40% mortality rate while CI (9.75 Gy)-animal group had 100% mortality rate. CI-induced sickness was accompanied by body weight loss, increased water intake, and delayed wound healing. At the 30th day post-injury, bone marrow cell depletion still remained in surviving CI mice. Treatment of CI (9.25 Gy)-animal group with BMSCs led to an increase in 30-day survival rate by 30%, attenuated body weight loss, accelerated wound healing rate, and ameliorated bone-marrow cell depletion. Our novel results are the first to suggest that BMSC therapy is efficacious to sustain animal survival after CI.</p>","PeriodicalId":73642,"journal":{"name":"Journal of cell science & therapy","volume":"5 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8396709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39364957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}