Diagnostic Accuracy and Predictive Value of Latex Agglutination Test, Rapid Cassette Test Compared to ELISA in Diagnosis of Toxoplasma gondii in Pregnant Sudanese Women

Abstract

Background: There are different procedures for the diagnosis of the pregnant female suspected with toxoplasmosis, however time, cost, and accuracy of the test should meat patients need. Material and Method: Three hundered pregnant female collected from Saad Abualila hospital antenatal care unit, were undergo three different procedures for the diagnosis of Toxoplasma gondii infection. Toxolatex. Toxo IgG-IgM rapid test and ELISA were done for all pregnant female. The result described as frequency and percentage of positivity, also specificity and sensitivity of Toxolatex. Toxo IgG-IgM rapid test were assessed according to ELISA results. Results: The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and diagnostic accuracy for Latex agglutination test to detect T. gondii antibodies were: 44.6%, 71.9%, 30.5%, 82.4% and 66. %, while the specificity, sensitivity, PPV, NPV and diagnostic accuracy for rapid cassette test to detect T. gondii antibodies were: 29.2%, 88.5%, 41.3%, 81.8% and 75.67%, respectively. Conclusion: Toxo IgG-IgM rapid test(casset) considered as good test for diagnosis of toxoplasmosis and mor specific than Toxolatex with also high diagnostic accuracy.