Advances in medical sciences最新文献_第2页

Corrigendum to “Effectiveness of antazoline versus amiodarone, flecainide and propafenone in restoring sinus rhythm at the emergency department” [Adv. Med. Sci (2024 Sep) 69(2) 248–255]

IF 2.5 4区医学

Advances in medical sciences Pub Date : 2025-02-08 DOI: 10.1016/j.advms.2025.02.003

Janusz Springer , Michalina Pejska , Wojciech Homenda , Tomasz Zdrojewski , Ludmiła Daniłowicz-Szymanowicz , Dariusz Kozłowski

引用次数: 0

Optimizing in vitro lung cancer therapy with folate-conjugated polydopamine-coated liposomes loaded with gemcitabine

IF 2.5 4区医学

Advances in medical sciences Pub Date : 2025-02-07 DOI: 10.1016/j.advms.2025.02.001

Chandramohan Govindasamy , Muhammad Ibrar Khan , Chitrakani Bose , Muruganantham Bharathi , Shamini Senthilkumar , Parthasarathy Surya

{"title":"Optimizing in vitro lung cancer therapy with folate-conjugated polydopamine-coated liposomes loaded with gemcitabine","authors":"Chandramohan Govindasamy , Muhammad Ibrar Khan , Chitrakani Bose , Muruganantham Bharathi , Shamini Senthilkumar , Parthasarathy Surya","doi":"10.1016/j.advms.2025.02.001","DOIUrl":"10.1016/j.advms.2025.02.001","url":null,"abstract":"<div><h3>Purpose</h3><div>Surface-altered, targeted nanocarriers play crucial roles in chemotherapy. Incorporating ligands into polymers may alter their chemical composition, potentially compromising their drug storage and encapsulation capacity. Polydopamine (PDA) is a novel, biocompatible, and versatile agent for producing targeted nanoparticles that serve as a base for conjugating specific ligands to non-reactive polymeric nanocarriers. This investigation aimed to evaluate whether gemcitabine (GEM)-loaded liposomes conjugated with PDA could enhance cancer treatment.</div></div><div><h3>Materials and methods</h3><div>A series of liposomes, named plain GEM, GEM@FA, and GEM@FA/PDA, was designed. Transmission Electron Microscopy (TEM), Fourier Transform Infrared Spectroscopy (FTIR), and X-ray Photoelectron Spectroscopy (XPS) were used to confirm the presence of PDA coating and folic acid (FA) and PDA conjugations. Cellular uptake, cytotoxicity, and cell death were evaluated using biochemical and flow cytometric assays.</div></div><div><h3>Results</h3><div>Compared to typical liposomes, GEM@FA/PDA liposomes were smaller, more stable, and exhibited a spherical shape with excellent cellular uptake. GEM@FA and GEM@FA/PDA liposomes showed significantly higher cytotoxicity against lung cancer (H1299) cells compared to GEM liposomes and pure GEM solution at all concentrations, while causing much less cytotoxicity to normal cells (NIH3T3).</div></div><div><h3>Conclusions</h3><div>GEM@FA/PDA liposomes demonstrated enhanced cancer-fighting effectiveness while minimizing harm to healthy tissues, making them a promising approach for chemotherapy.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 1","pages":"Pages 141-151"},"PeriodicalIF":2.5,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The association between circulating irisin, osteocalcin and FGF21 levels with anthropometric characteristics and blood lipid profile in young obese male subjects

IF 2.5 4区医学

Advances in medical sciences Pub Date : 2025-01-31 DOI: 10.1016/j.advms.2025.01.010

Sema Sayharman, Muaz Belviranlı, Nilsel Okudan

{"title":"The association between circulating irisin, osteocalcin and FGF21 levels with anthropometric characteristics and blood lipid profile in young obese male subjects","authors":"Sema Sayharman, Muaz Belviranlı, Nilsel Okudan","doi":"10.1016/j.advms.2025.01.010","DOIUrl":"10.1016/j.advms.2025.01.010","url":null,"abstract":"<div><h3>Purpose</h3><div>Myokines secreted from skeletal muscle such as irisin, osteokines secreted from bone such as osteocalcin, and hepatokines secreted from the liver such as fibroblast growth factor 21 (FGF21) play a role in the regulation of metabolic homeostasis. However, the changes that occur in obesity and the interaction between them have not been fully explained. Therefore, this study aimed to compare irisin, osteocalcin and FGF21 levels in young obese males against individuals with normal body weight and to reveal the possible relationship between them and with anthropometric measurements and blood lipid profile.</div></div><div><h3>Materials and methods</h3><div>This single-center study included 28 Turkish young males aged 20–29 years: 14 obese participants with a body mass index (BMI) between 30.0 and 34.9 and 14 healthy controls with a BMI between 18.5 and 24.9. Anthropometric, and body composition parameters, blood lipid profile, and irisin, osteocalcin and FGF21 levels of groups were measured. Correlation analyses were performed between irisin, osteocalcin, and FGF21 and other measured parameters.</div></div><div><h3>Results</h3><div>Circulating irisin, osteocalcin and FGF21 levels were significantly higher in the obese group than in the control group (p < 0.05). Correlation analysis showed that irisin was positively correlated with total cholesterol, triglycerides and low density lipoprotein-cholesterol (LDL-C) and FGF21 was positively correlated with total cholesterol and LDL-C (p < 0.05). Positive correlation between irisin and osteocalcin, FGF21 and osteocalcin and FGF21 and irisin was observed (p < 0.05).</div></div><div><h3>Conclusions</h3><div>Irisin, osteocalcin, and FGF21 have a potential role in the pathophysiology of obesity and related metabolic diseases due to their interactions.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 1","pages":"Pages 117-123"},"PeriodicalIF":2.5,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of TRPM7 by glutathione decreases oxidant and apoptotic action of cisplatin through the downregulation of Ca2+ and Zn2+ in glioblastoma cells

IF 2.5 4区医学

Advances in medical sciences Pub Date : 2025-01-30 DOI: 10.1016/j.advms.2025.01.008

Kemal Ertilav , Mustafa Nazıroğlu

{"title":"Inhibition of TRPM7 by glutathione decreases oxidant and apoptotic action of cisplatin through the downregulation of Ca2+ and Zn2+ in glioblastoma cells","authors":"Kemal Ertilav , Mustafa Nazıroğlu","doi":"10.1016/j.advms.2025.01.008","DOIUrl":"10.1016/j.advms.2025.01.008","url":null,"abstract":"<div><h3>Purpose</h3><div>Cisplatin (CiSP)-mediated stimulation of TRPM7 may induce oxidant and apoptotic activities through the upregulation of Ca<sup>2+</sup>, apoptosis, and reactive oxygen species (ROS) in glioblastoma (DBTRG-05MG) cells, whereas inhibition of TRPM7 by the antioxidant glutathione (GSH) may reduce the observed increases in DBTRG-05MG. The aim of the study was to examine how TRPM7 activation stimulates DBTRG-05MG cell death but also how it inhibits the effects of TRPM7 antagonists (GSH and carvacrol, CRV) via altering ROS toxicity and apoptosis.</div></div><div><h3>Method</h3><div>In the DBTRG-05MG, 5 groups were established: control, GSH (10 mM for 2 h), CiSP (25 μM for 24 h), CiSP + GSH, and CiSP + CRV (200 μM for 24 h).</div></div><div><h3>Results</h3><div>The amounts of cytosolic free Ca<sup>2+</sup> were further increased in the CiSP group by the stimulation of TRPM7 (naltriben), even though the GSH and CRV treatments caused them to decrease in the cells. The amounts of mitochondrial membrane dysfunction, ROS, death cell, apoptosis, free zinc ion, and caspase-3, -8, and -9 in the cells were higher in the CiSP than in the control and GSH, although their amounts were lower in the CiSP + GSH and CiSP + CRV than in the CiSP only. The CiSP-induced decreases in cell viability and GSH concentrations were increased by GSH incubation.</div></div><div><h3>Conclusions</h3><div>The stimulation of TRPM7 increased the anticancer action of CiSP, although its inhibition decreased the amount of CiSP-induced oxidative stress and DBTRG-05MG deaths through the treatment of GSH and CRV. TRPM7 stimulation could be considered a potential tumor killer channel through oxidative glioblastoma damage caused by CiSP.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 1","pages":"Pages 124-135"},"PeriodicalIF":2.5,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel factors of cisplatin resistance in epithelial ovarian tumours

IF 2.5 4区医学

Advances in medical sciences Pub Date : 2025-01-27 DOI: 10.1016/j.advms.2025.01.005

Pavol Harvanik , Martina Šemeláková , Zuzana Solárová , Peter Solár

引用次数: 0

Circadian and autophagy markers correlate with poor prognosis in meningioma patients 昼夜节律和自噬标记与脑膜瘤患者预后不良有关

IF 2.5 4区医学

Advances in medical sciences Pub Date : 2025-01-23 DOI: 10.1016/j.advms.2025.01.006

Keng-Liang Kuo , Shu-Jyuan Chang , Cheng Yu Tsai , Yen-Shuo Huang , Aij-Lie Kwan , Chee-Yin Chai

{"title":"Circadian and autophagy markers correlate with poor prognosis in meningioma patients","authors":"Keng-Liang Kuo , Shu-Jyuan Chang , Cheng Yu Tsai , Yen-Shuo Huang , Aij-Lie Kwan , Chee-Yin Chai","doi":"10.1016/j.advms.2025.01.006","DOIUrl":"10.1016/j.advms.2025.01.006","url":null,"abstract":"<div><h3>Purpose</h3><div>Patients with meningiomas mostly present good outcomes and optimal prognosis, but different grades of tumors have very different symptoms and recurrence rates. Therefore, effective diagnosis is crucial for early intervention and controlling tumor development. Circadian cycle and autophagy have both been proven to be related to neoplasm formation and pathogenesis; however, there is limited exploration and discussion on the relationships between the circadian cycle and autophagy in patients with meningiomas. This study was undertaken to elucidate the relationship between two autophagy markers (Beclin1, LC3B) and one circadian marker (NR1D1) with clinicopathological parameters in patients with meningiomas.</div></div><div><h3>Materials and methods</h3><div>Clinicopathological data of 124 enrolled patients were collected. Tissue-sectioned slides were analyzed via immunohistochemical stains and the relationship between the markers was evaluated.</div></div><div><h3>Results</h3><div>Individually low expression of NR1D1 and Beclin 1 was associated with better prognosis, lower pathological grade, and longer survival. Although correlation analysis showed that NR1D1, Beclin 1 and LC3B were related to each other. However, the dual marker NR1D1-/Beclin 1- was effective in predicting good prognosis in meningiomas, whereas NR1D1-/LC3B- was not.</div></div><div><h3>Conclusion</h3><div>NR1D1 and Beclin 1 could be adopted as a single marker or coupled as a combined marker to predict meningioma prognoses, pathological grades, and survival. This study provides insights into the association between autophagy and circadian cycles and may benefit future elucidation of molecular mechanisms.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 1","pages":"Pages 103-108"},"PeriodicalIF":2.5,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and temporal-validation of prognostic models for 5-year risk of pneumonia, respiratory failure/collapse, and fracture among adults with cerebral palsy 针对脑瘫成人肺炎、呼吸衰竭/塌陷和骨折 5 年风险的预后模型的开发和时间验证。

IF 2.5 4区医学

Advances in medical sciences Pub Date : 2025-01-22 DOI: 10.1016/j.advms.2025.01.007

Daniel G. Whitney

{"title":"Development and temporal-validation of prognostic models for 5-year risk of pneumonia, respiratory failure/collapse, and fracture among adults with cerebral palsy","authors":"Daniel G. Whitney","doi":"10.1016/j.advms.2025.01.007","DOIUrl":"10.1016/j.advms.2025.01.007","url":null,"abstract":"<div><h3>Purpose</h3><div>Pneumonia, respiratory failure, and fracture are common and highly burdensome for adults with cerebral palsy (CP). To date, there are no clinically friendly tools to assess individualized risk of these outcomes for adults with CP. The objective was to develop and validate prognostic models for 5-year risk of pneumonia, respiratory failure/collapse, and fracture for adults with CP.</div></div><div><h3>Patients and methods</h3><div>This single medical center retrospective cohort study used medical records from January 1, 2012 till June 1, 2024 from adults ≥18 years old with CP. The development cohort (n = 1520) included those with a start date of follow-up from January 1, 2015 till December 31, 2015 and evidence of being treated at the medical center for ≥3 years prior to day 0. The 5-year risk of outcomes was modelled using logistic regression and variations of the following predictors that were collected during the 3-year baseline: age, sex, Whitney Comorbidity Index, pneumonia, respiratory failure/collapse, fracture, and asthma/COPD. Discrimination (c-statistic) and calibration statistics assessed the model's performance. Decision curve analysis assessed the model's clinical utility. The models were validated in a temporal validation cohort, whose start date of follow-up was January 1, 2016 to May 31, 2019 (n = 529).</div></div><div><h3>Results</h3><div>The prognostic models had good discrimination (c-statistic = 0.76–0.78), good-to-excellent calibration, and demonstrated superior clinical utility in identifying true positives and true negatives. All models demonstrated temporal validation.</div></div><div><h3>Conclusions</h3><div>Prognostic models for 5-year risk of outcomes were developed and temporally validated for adults with CP using measures that can be easily collected from medical records.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 1","pages":"Pages 109-116"},"PeriodicalIF":2.5,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety and efficacy of therapeutic plasma exchange in pediatric neuro-immunological diseases 血浆置换治疗小儿神经免疫疾病的安全性和有效性。

IF 2.5 4区医学

Advances in medical sciences Pub Date : 2025-01-15 DOI: 10.1016/j.advms.2025.01.003

Magdalena Błasiak , Przemysław Korohoda , Katarzyna Zachwieja , Dorota Drożdż , Aleksandra Gergont , Karina Madej-Świątkowska , Monika Miklaszewska

{"title":"Safety and efficacy of therapeutic plasma exchange in pediatric neuro-immunological diseases","authors":"Magdalena Błasiak , Przemysław Korohoda , Katarzyna Zachwieja , Dorota Drożdż , Aleksandra Gergont , Karina Madej-Świątkowska , Monika Miklaszewska","doi":"10.1016/j.advms.2025.01.003","DOIUrl":"10.1016/j.advms.2025.01.003","url":null,"abstract":"<div><h3>Purpose</h3><div>Therapeutic plasma exchange (TPE) is the treatment of choice in various neuro-immunological disorders in pediatric populations. This study assesses the safety and efficacy of TPE in this demographic.</div></div><div><h3>Materials and methods</h3><div>A single-center, retrospective cohort study involving pediatric patients who experienced neuro-immunological events and underwent TPE procedures at a tertiary referral center was conducted.</div></div><div><h3>Results</h3><div>The study included 81 patients (Guillain-Barre: 65, polyneuropathies: 5, myasthenia gravis: 8, multiple sclerosis: 3), undergoing collectively 360 TPE procedures. Fresh frozen plasma (FFP) was used in 76.1 % of the TPE procedures. Adverse events (AEs) occurred in 50 % of TPEs using FFP compared to 39.5 % without. For procedures with at least two AEs, the rates were 24.5 % with FFP vs 8.1 % without. Incidence of allergic AEs was significantly higher in the FFP group (94.2 % of TPE with at least one AE) compared to those without FFP (47.2 %). Serious AE accounted for 1.2 % of TPE procedures and 2.5 % of patients. Effectiveness evaluations using a scale developed for this study and the Hughes Functional Grading Scale showed no significant clinical pre-treatment differences. After completing the treatment, children in the polyneuropathies group had the most severe clinical condition, and the largest relative improvement in clinical status was in the myasthenia gravis group.</div></div><div><h3>Conclusions</h3><div>TPE conducted by filtration is an effective and safe therapy for pediatric neuro-immunological diseases, with benefits outweighing the risks of complications. The use of FFP in therapy increases the probability of AE by 27 %, and significantly raises the risk of allergic and multiple AEs.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 1","pages":"Pages 86-93"},"PeriodicalIF":2.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of circulating natural autoantibodies against ANXA1 and MYC as potential biomarkers in hepatocellular carcinoma 抗ANXA1和MYC的循环天然自身抗体作为肝细胞癌潜在生物标志物的研究。

IF 2.5 4区医学

Advances in medical sciences Pub Date : 2025-01-15 DOI: 10.1016/j.advms.2025.01.004

Jiaxin Wang , Siqi Liu , Xuan Zhang

{"title":"Investigation of circulating natural autoantibodies against ANXA1 and MYC as potential biomarkers in hepatocellular carcinoma","authors":"Jiaxin Wang , Siqi Liu , Xuan Zhang","doi":"10.1016/j.advms.2025.01.004","DOIUrl":"10.1016/j.advms.2025.01.004","url":null,"abstract":"<div><h3>Purpose</h3><div>In this study, we examined novel autoantibodies targeting tumor-associated antigens (TAAs) as biomarkers for clinical assessment of hepatocellular carcinoma (HCC) in a Chinese population.</div></div><div><h3>Methods and methods</h3><div>A total of 119 patients with HCC and 130 healthy control (HC) volunteers who were age and gender matched were enrolled. The levels of circulating IgG antibodies were detected using an enzyme-linked immunosorbent test (ELISA) developed in-house with linear peptide antigens derived from Annexin A1(ANXA1) and proto-oncogene protein (MYC). The significant level was set at <em>P</em> < 0.025 as two tests were performed.</div></div><div><h3>Results</h3><div>In comparison to the HC group, plasma level of ANXA1 autoantibodies was significantly elevated in HCC patients (<em>t</em> = −3.174, <em>P</em> = 0.002) but the change of plasma MYC autoantibody levels failed to reach the significance level (<em>P</em> > 0.025). There was a significant increase in these two plasma IgG autoantibodies in male HCC patients (ANXA1: <em>t</em> = −3.590, <em>P</em> < 0.001; MYC: <em>t</em> = −2.706, <em>P</em> = 0.007). Pearson correlation analysis demonstrated that both anti-ANXA1 and anti-MYC IgG levels had a positive correlation with BCLC staging (both <em>P</em> < 0.025) but a negative correlation with plasma albumin (Alb) (both <em>P</em> < 0.025). The area under the ROC curve (AUC) values were 0.613 for anti-ANXA1 IgG assay and 0.567 for anti-MYC IgG assay. The anti-ANAXA1 IgG assay showed a high sensitivity of 31.4 % against the specificity of 90.0 % for detection of BCLC stages C + D.</div></div><div><h3>Conclusions</h3><div>Plasma anti-ANXA1 and anti-MYC autoantibodies are likely to serve as a potential biomarker for clinical assessment of HCC prognosis, particularly in male patients.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 1","pages":"Pages 136-140"},"PeriodicalIF":2.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulation and inhibition of TRPM2 calcium channel prevent oxidative stress-induced endothelial dysfunction in the EA.hy926 endothelial cells model - Preliminary studies 下调和抑制TRPM2钙通道可预防EA.hy926内皮细胞模型中氧化应激诱导的内皮功能障碍——初步研究

IF 2.5 4区医学

Advances in medical sciences Pub Date : 2025-01-06 DOI: 10.1016/j.advms.2025.01.002

Wioletta Arendt , Klaudia Piekarska , Marta Hałas-Wiśniewska , Magdalena Izdebska , Alina Grzanka , Maciej Gagat

{"title":"Downregulation and inhibition of TRPM2 calcium channel prevent oxidative stress-induced endothelial dysfunction in the EA.hy926 endothelial cells model - Preliminary studies","authors":"Wioletta Arendt , Klaudia Piekarska , Marta Hałas-Wiśniewska , Magdalena Izdebska , Alina Grzanka , Maciej Gagat","doi":"10.1016/j.advms.2025.01.002","DOIUrl":"10.1016/j.advms.2025.01.002","url":null,"abstract":"<div><h3>Purpose</h3><div>Proper functioning of the endothelial barrier is crucial for cardiovascular system homeostasis. Oxidative stress can lead to endothelial dysfunction (ED), damaging lipids, proteins, and DNA. Reactive oxygen species also increase cytoplasmic Ca<sup>2+</sup> levels, activating transient receptor potential melastatin 2 (TRPM2), a membrane non-selective calcium channel. The study aimed to assess TRPM2's significance in vascular endothelial cells' response to oxidative stress and the potential use of TRPM2 direct and indirect inhibitors in the prevention of oxidative stress-induced ED.</div></div><div><h3>Materials and methods</h3><div>EA.hy926 endothelial cells were exposed to hydrogen peroxide for 24 h to mimic oxidative stress conditions. To assess the significance of TRPM2 in the response of EA.hy926 cells to hydrogen peroxide TRPM2 siRNA as well as direct (N-(p-Amylcinnamoyl)anthranilic acid, flufenamic acid) and indirect (3-aminobenzamide, 3,4-dihydro-5[4-(1-piperidinyl)butyl]-1(2H)-isoquinolinone) TRPM2 inhibitors were tested.</div></div><div><h3>Results</h3><div>Results showed that hydrogen peroxide-induced ED is alleviated by TRPM2 downregulation. Moreover, preincubation of cells with both direct and indirect TRPM2 inhibitors for 30 min before hydrogen peroxide treatment reduces its negative effects on cell viability, cell migration, and junctional proteins.</div></div><div><h3>Conclusions</h3><div>The obtained results suggest that TRPM2 channel may be a potential target in therapy and prevention of cardiovascular diseases connected with oxidative stress-induced ED. However, further research is needed for clinical applications of direct and indirect TRPM2 inhibitors.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 1","pages":"Pages 62-78"},"PeriodicalIF":2.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0