Advances in medical sciences最新文献

{"title":"Angiopoietin-like 4 facilitates human aortic smooth muscle cell phenotype switch and dysfunctions through the PI3K/Akt signaling in aortic dissection","authors":"Wei He ,&nbsp;Quan Zheng ,&nbsp;Tingfang Zou ,&nbsp;Wei Yan ,&nbsp;Xue Gao ,&nbsp;Chunle Wang ,&nbsp;Yaoyao Xiong","doi":"10.1016/j.advms.2024.09.006","DOIUrl":"10.1016/j.advms.2024.09.006","url":null,"abstract":"<div><h3>Purpose</h3><div>Vascular smooth muscle cell (VSMC) phenotype switch and dysfunctions have been reported to participate in aortic dissection (AD) progression. This study was aimed to investigate the role of angiopoietin-like 4 (ANGPTL4) in regulating VSMCs phenotype switch.</div></div><div><h3>Materials and methods</h3><div>Key genes were analyzed in AD using public datasets, and it was found that the central differential gene <em>ANGPTL4</em> was up-regulated in AD. The KEGG signaling pathway annotation was performed to validate the associated pathways, and the expression of <em>ANGPTL4</em> was verified using multiple datasets and clinical samples. Furthermore, the specific functions of ANGPTL4 on platelet-derived growth factor-BB (PDGF-BB)-treated human aortic smooth muscle cell (HASMC) phenotypes were investigated. The dynamic effects of ANGPTL4 and core signaling antagonists on HASMC phenotypes were examined.</div></div><div><h3>Results</h3><div>Hub gene <em>ANGPTL4</em> was significantly up-regulated in AD. <em>ANGPTL4</em> was linked to the PI3K/Akt signaling, angiogenesis, and neovascularization and remodeling. <em>ANGPTL4</em> overexpression further enhanced PDGF-BB effects on HASMC phenotypes, including promoted cell viability and migration, decreased contractile VSMC markers α-SMA and SM22α, elevated ECM degradation markers MMP-2 and MMP-9, and promoted phosphorylation of PI3K and Akt. <em>ANGPTL4</em> knockdown partially abolished PDGF-BB-induced contractile/synthetic VSMCs imbalance and HASMC dysfunctions. Furthermore, in <em>ANGPTL4</em>-overexpressing HASMCs pre-treated with PDGF-BB, the PI3K/Akt signaling inhibitor LY294002 also partially eliminated the effects caused by the PDGF-BB treatment and <em>ANGPTL4</em> overexpression.</div></div><div><h3>Conclusions</h3><div><em>ANGPTL4</em> is significantly up-regulated in AD. <em>ANGPTL4</em> overexpression further enhanced PDGF-BB effects on HASMC phenotype switch and dysfunctions, which might be involved in the PI3K/Akt signaling.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 474-483"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142339163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reducing metastasis ability of gastric cancer cell line by targeting MMP16 using miR-193a-5p and 5-FU","authors":"Tahani Ahmad Almatrafi ,&nbsp;Natrayan Lakshmaiya ,&nbsp;Hailah M. Almohaimeed ,&nbsp;Srikumar Chakravarthi ,&nbsp;Ali H. Amin ,&nbsp;Ayman Jafer ,&nbsp;Amany I. Almars ,&nbsp;Ammar A. Basabrain ,&nbsp;Youssef S. Alghamdi ,&nbsp;Mohamed J. Saadh ,&nbsp;Reza Akhavan-Sigari","doi":"10.1016/j.advms.2024.09.008","DOIUrl":"10.1016/j.advms.2024.09.008","url":null,"abstract":"<div><h3>Purpose</h3><div>Co-administration of microRNAs and chemotherapy drugs effectively treats several cancers. The current study sought to investigate the function of matrix metalloproteinase 16 (MMP16) and miR-193a-5p in the pathogenesis of gastric cancer (GC).</div></div><div><h3>Materials/methods</h3><div>Sixty-five surgical patients, 15 receiving 5-fluorouracil (5-FU), provided GC and adjacent non-cancerous tissue. Following that, qPCR was used to assess the expression levels of MMP16 and miR-193a-5p in GC cells. The impact of miR-193a-5p and 5-FU administration on MMP16 mRNA expression was evaluated using qRT-PCR and Western blotting. MTT and Scratch tests were also conducted to assess their effects on cell viability and migration. Moreover, a rescue experiment using an MTT assay was performed. Using flow cytometry, the apoptotic rate was calculated. Finally, it was evaluated how MMP16 and miR-193a-5p related to the clinicopathological characteristics of the patients.</div></div><div><h3>Results</h3><div>The current study found that while MMP16 expression increased in GC patients (P ​&lt; ​0.0001), miR-193a-5p expression significantly decreased (P ​&lt; ​0.001). MMP16 down-regulation was another effect of miR-193a-5p replacement, particularly when 5-FU was added (P ​&lt; ​0.01). In addition, this study found that miR-193a-5p, by concentrating on MMP16, decreased the migration of GC cells brought on by MMP16. In GC cell lines, miR-193 and 5-FU induce apoptosis, with the 5-FU being more pronounced when combined with mir-193, according to flow cytometry results. A strong correlation was also found between clinicopathological traits associated with MMP16 and miR-193a-5p.</div></div><div><h3>Conclusions</h3><div>These findings suggest that miR-193a-5p, in conjunction with 5-FU, down-regulates MMP16 in GC, where it suppresses tumor growth.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 463-473"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142339165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LRRC45 promotes lung cancer proliferation and progression by enhancing c-MYC, slug, MMP2, and MMP9 expression","authors":"Qian Wang ,&nbsp;Xin-Yan Liu ,&nbsp;Xiao-Qi Zhang ,&nbsp;Zheng-Xing Huo ,&nbsp;Cheng-Yu Chen ,&nbsp;Shi Chen ,&nbsp;Cheng-Yong Liu ,&nbsp;Jia Zhu ,&nbsp;Shan-Shan Liu ,&nbsp;Bing Lu","doi":"10.1016/j.advms.2024.09.007","DOIUrl":"10.1016/j.advms.2024.09.007","url":null,"abstract":"<div><h3>Background</h3><div>The leucine-rich repeat-containing (LRRC) superfamily members are known for their significant roles in tumorigenesis and cellular proliferation. However, the specific regulatory role of LRRC45 in lung cancer remains unexplored. This study investigated the impact and underlying mechanisms of LRRC45 on the proliferative, migratory, and invasive capacities of lung adenocarcinoma (LUAD) cells, potentially identifying new targets for therapeutic intervention.</div></div><div><h3>Material and methods</h3><div>The importance of LRRC45 in lung cancer was analyzed using the online databases of UCSC Xena, TCGA, TISIDB, and UALCAN, whereas to detect target gene expression, we used the qRT-PCR, Western blot, and immunofluorescence confocal. The cell growth was monitored by colony formation assay and migration was examined by cell migration assay. Finally, a xenograft mouse tumor model using A549 ​cells was used to explore the <em>in vivo</em> effect of LRRC45 in lung cancer.</div></div><div><h3>Results</h3><div>Inhibition of LRRC45 expression led to a notable decrease in proliferation, migration, and invasion of A549 and H1299 ​cells. LRRC45 silencing significantly reduced the tumor volume and improved the mice's survival. Additionally, inhibition of LRRC45 expression dramatically suppressed c-MYC, Slug, MMP2, and MMP9 expression. Overexpression of c-MYC and/or Slug in the LRRC45-deficient cells can partially or totally restore the LRRC45 deficiency-suppressed growth. Moreover, the overexpression of MMP2 and/or MMP9 could partially or totally restore LRRC45 deficiency-reduced cell metastasis.</div></div><div><h3>Conclusions</h3><div>LRRC45 could promote the proliferative, migrative, and invasive capacities of lung cancer cells by increasing c-MYC, Slug, MMP2, and MMP9 expression, indicating the therapeutic implications and potential significance of these pathways in lung cancer.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 451-462"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142339164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in hematopoietic stem cell numbers following acute exercise in non-athlete marathon runners","authors":"Özgür Günaştı ,&nbsp;Çiğdem Özdemir ,&nbsp;Kerem T. Özgünen ,&nbsp;Gizem Çiftdal ,&nbsp;Ertuğrul Gezgin ,&nbsp;Selcen Korkmaz Eryılmaz ,&nbsp;Ömer Cumhur Boyraz ,&nbsp;Abdullah Kılcı ,&nbsp;Ümüt Adaş ,&nbsp;Bülent Antmen ,&nbsp;Sanlı Sadi Kurdak","doi":"10.1016/j.advms.2024.09.003","DOIUrl":"10.1016/j.advms.2024.09.003","url":null,"abstract":"<div><h3>Purpose</h3><p>Hematopoietic stem cell (HSC) transplant is one of the curative methods for some patients with hematological malignancies. Granulocyte colony-stimulating factor (G-CSF) is the most common drug used to mobilize CD34⁺ cells, generally found in small numbers. Recent evidence showed that exercise causes transient mobilization in HSC. However, the type and intensity of exercise have not been fully revealed. We aimed to detect a significant increase in stem cell levels following 60 ​min of running at a personalized running pace.</p></div><div><h3>Materials/methods</h3><p>Eighteen runners, 48.2 ​± ​1.9 years with peak oxygen consumption of 46.2 ​± ​1.4 ​ml/kg/min, were enrolled in the study. The cardiopulmonary exercise test was performed to determine the individual running pace, and the participants ran 60-min on a treadmill at an intensity close to their ventilatory threshold (VT). The blood sampling for HSC count was performed before, immediately after, at the 1st, 4th and 24th hour after the 60-min running.</p></div><div><h3>Results</h3><p>The CD34⁺ HSCs were 13.9 ​± ​2.3 ​cells/μl before and significantly increased immediately after to 19.5 ​± ​3.6 ​cells/μl (p ​&lt; ​0.05). The consecutive HSC counts were 15.3 ​± ​2.2, 19.5 ​± ​4.8 and 15.1 ​± ​3.4 ​cells/μl at the 1st, 4th, and 24th hour, respectively.</p></div><div><h3>Conclusion</h3><p>The individual data showed that some runners had higher HSC levels than the transplantation limit before and after the 60-min running trail, which was maintained for 24 ​h. Pre-running high CD34⁺ HSCs may reflect an adaptive response to regular exercise, with a 60-min run near the VT further elevating HSCs. Individualized exercise may be a valuable tool to mobilize the CD34⁺ HSCs in peripheral blood for donors.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 416-420"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pentraxin 3 as a marker of development and severity of stable coronary artery disease","authors":"Malgorzata Knapp,&nbsp;Monika Gil-Mika,&nbsp;Robert Sawicki,&nbsp;Anna Lisowska,&nbsp;Marcin Kaminski,&nbsp;Bozena Sobkowicz,&nbsp;Katarzyna Ptaszynska","doi":"10.1016/j.advms.2024.07.010","DOIUrl":"10.1016/j.advms.2024.07.010","url":null,"abstract":"<div><h3>Purpose</h3><p>Inflammation plays a crucial role in the development of atherosclerotic plaques. Pentraxin 3 (PTX3) is produced at the site of inflammation and has been identified as a specific marker of atherosclerosis, vascular inflammation, and progression of the coronary artery disease (CAD).</p><p>The aim of the study was to establish if PTX3 has potential relations with classical markers of cardiovascular risk, and if PTX3 may act as an independent risk factor of CAD occurrence and advancement.</p></div><div><h3>Materials and methods</h3><p>The study included 98 patients with stable CAD confirmed in coronary angiography (CAD group) (median age 65 interquartile range [IQR] 61–72 years; 72 ​% men). The control group consisted of 40 patients without CAD.</p></div><div><h3>Results</h3><p>The CAD group had significantly higher PTX3 concentration compared to the control group. There was a correlation with age, male gender, lipid profile and intima-media thickness. There was no correlation between PTX3 concentration and the number of coronary vessels with significant atherosclerotic lesions and the advancement of atherosclerotic lesions on the Gensini scoring scale. The cut-off point was determined for 0.89 ​ng/ml for the exclusion of angiographically significant atherosclerotic lesions.</p></div><div><h3>Conclusions</h3><p>Patients with CAD have significantly higher concentration of PTX3. There was no correlation between PTX3 and the advancement of angiographically significant atherosclerotic lesions in coronary arteries. Low PTX3 concentration may serve as an indicator for the absence of atherosclerosis.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 391-397"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141791647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Right-ventricle heart failure in PAH vs. HFrEF with secondary PH: Hemodynamic, ergospirometric, and organ function correlations","authors":"Katarzyna Krajewska,&nbsp;Krystian Ejdys,&nbsp;Klaudia Jadczak,&nbsp;Anna Lisowska,&nbsp;Karol A. Kamiński,&nbsp;Bożena Sobkowicz,&nbsp;Katarzyna Ptaszyńska","doi":"10.1016/j.advms.2024.08.003","DOIUrl":"10.1016/j.advms.2024.08.003","url":null,"abstract":"<div><h3>Purpose</h3><div>The goal of the study was to identify markers of organ function used in daily routines that could potentially aid in the overall evaluation of the cardiovascular system in patients with right-ventricle heart failure due to pulmonary arterial hypertension (PAH) and left-ventricle heart failure. We analyzed correlations between parameters from right heart catheterization (RHC), cardiopulmonary exercise test (CPET), and selected laboratory parameters of thyroid, liver, kidneys function and iron homeostasis.</div></div><div><h3>Patients and methods</h3><div>A retrospective analysis included 107 patients (mean age 57.6 ​± ​16.2; 34.6 ​% women), comprising 57 patients with PAH (mean age 54.0 ​± ​18.2; 49.1 ​% women) and 50 patients with heart failure with reduced ejection fraction (HFrEF) ​&lt; ​40 ​% (mean age 61.6 ​± ​12.7; 18 ​% women). All patients underwent CPET. Each patient in the PAH group had RHC performed. Fifteen patients from the HFrEF group underwent RHC, which confirmed the suspicion of pulmonary hypertension (HFrEF-SPH).</div></div><div><h3>Results</h3><div>CPET and laboratory parameters’ analysis showed strong correlations between ventilation/carbon dioxide production (VE/VCO₂) slope and NT-proBNP in HFrEF without secondary PH and HFrEF-SPH groups. In the PAH group, VE/VCO₂ slope correlated with liver and thyroid function but also with morphological parameters of red-cell system.</div><div>Analysis of correlations between laboratory and hemodynamic parameters revealed significant correlations between pulmonary arterial pressure, pulmonary vascular resistance (PVR) and red-cell parameters, especially strong with fT4 in the PAH group.</div></div><div><h3>Conclusions</h3><div>In HFrEF-SPH patients, laboratory parameters strongly correlated with pulmonary pressures and pulmonary capillary wedge pressure (PCWP).</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 421-427"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The vitamin D analog EB1089 sensitizes triple-negative breast cancer cells to the antiproliferative effects of antiestrogens","authors":"Adriana Zárate-Pérez ,&nbsp;Alitzin Pamela Cruz-Cázares ,&nbsp;David Ordaz-Rosado ,&nbsp;Janice García-Quiroz ,&nbsp;Alfonso León-Del-Rio ,&nbsp;Euclides Avila ,&nbsp;Edgar Milo-Rocha ,&nbsp;Lorenza Díaz ,&nbsp;Rocío García-Becerra","doi":"10.1016/j.advms.2024.08.004","DOIUrl":"10.1016/j.advms.2024.08.004","url":null,"abstract":"<div><h3>Purpose</h3><p>Patients bearing estrogen receptor (ER)α-negative breast cancer tumors confront poor prognosis and are typically unresponsive to hormone therapy. Previous studies have shown that calcitriol, the active vitamin D metabolite, can induce ERα expression in ERα-negative cells. EB1089, a calcitriol analog with reduced calcemic effects, exhibits greater potency than calcitriol in inhibiting cancer cell growth. However, the impact of EB1089 on ERα expression in triple-negative breast cancer (TNBC) cells remains unexplored. This study aims to investigate whether EB1089 could induce functional ERα expression in TNBC cell lines, potentially enabling the antiproliferative effects of antiestrogens.</p></div><div><h3>Materials and methods</h3><p>TNBC cell lines HCC1806 and HCC1937 were treated with EB1089, and ERα expression was analyzed using real-time PCR and Western blots. The transcriptional activity of induced ERα was evaluated through a luciferase reporter assay. The antiproliferative effects of tamoxifen and fulvestrant antiestrogens were assessed using the sulforhodamine B assay in the EB1089-treated cells.</p></div><div><h3>Results</h3><p>Our findings indicated that EB1089 significantly induced ERα mRNA and protein expression in TNBC cells. Moreover, EB1089-induced ERα exhibited transcriptional activity and effectively restored the inhibitory effects of antiestrogens, thereby suppressing cell proliferation in TNBC cells.</p></div><div><h3>Conclusion</h3><p>EB1089 induced the expression of functional ERα in TNBC cells, restoring the antiproliferative effects of antiestrogens. These results highlight the potential of using EB1089 as a promising strategy for re-establishment of the antiproliferative effect of antiestrogens as a possible management for TNBC. This research lays the foundation for potential advancements in TNBC treatment, offering new avenues for targeted and effective interventions.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 398-406"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between sleep disorders and clinical outcomes of patients with primary biliary cholangitis","authors":"Shijing Dong ,&nbsp;Simin Zhou ,&nbsp;Jiangpeng Liu ,&nbsp;Nian Chen,&nbsp;Jiwen Li,&nbsp;Zongze Han,&nbsp;Ruiyun Liu,&nbsp;Chenyang Xuan,&nbsp;Weirong Wang,&nbsp;Liping Guo,&nbsp;Lu Zhou","doi":"10.1016/j.advms.2024.08.002","DOIUrl":"10.1016/j.advms.2024.08.002","url":null,"abstract":"<div><h3>Purpose</h3><p>Primary biliary cholangitis (PBC) is a chronic autoimmune liver disease characterized by a range of symptoms, including sleep disturbances. The present study aimed to investigate the prevalence of sleep disorders and the associations between sleep disorders and clinical outcomes in PBC.</p></div><div><h3>Patients and methods</h3><p>We enrolled 177 patients with PBC and 165 healthy controls (age- and sex-matched). Sleep quality was assessed by the Pittsburgh Sleep Quality Index (PSQI). Demographic and clinical data were collected from comprehensive clinical records to investigate whether sleep disorder was correlated with disease severity, therapeutic response and liver cirrhosis.</p></div><div><h3>Results</h3><p>The prevalence of sleep disorders in patients with PBC (50.8 ​%) was significantly higher than healthy controls (18.2 ​%). Patients with sleep disorders presented with higher levels of laboratory parameters including globulin (GLO), aspartate aminotransferase (AST), alkaline phosphatase (ALP), glutamyl transpeptidase (GGT), total bilirubin (TBIL), direct bilirubin (DBIL) and immunoglobulin M (IgM), as well as higher ratio of poor therapeutic response and liver cirrhosis (<em>p</em> ​&lt; ​0.05). There was a positive correlation between global PSQI score and AST, ALP, GGT, TBIL, DBIL and IgM in patients with PBC. Patients with poor therapeutic response and liver cirrhosis in PBC had a higher proportion of sleep disorders and more chaotic sleep patterns, whereas a stronger correlation between sleep quality and laboratory parameters was found in patients with liver cirrhosis.</p></div><div><h3>Conclusions</h3><p>Sleep disorders were prevalent and manifested as adverse effects in PBC. Assessment of sleep quality and intervention were essential to the overall clinical management of patients with PBC.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 385-390"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1896112624000464/pdfft?md5=b0492704685fc4e303759be7a34651df&pid=1-s2.0-S1896112624000464-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentiation of solid and friable tumour thrombus in patients with renal cell carcinoma: The role of MRI apparent diffusion coefficient","authors":"Paweł Kowal ,&nbsp;Krzysztof Ratajczyk ,&nbsp;Wiktor Bursiewicz ,&nbsp;Maciej Trzciniecki ,&nbsp;Karolina Marek-Bukowiec ,&nbsp;Joanna Rogala ,&nbsp;Vasyl Kowalskyi ,&nbsp;Jozef Dragasek ,&nbsp;Andrea Botikova ,&nbsp;Peter Kruzliak ,&nbsp;Yulian Mytsyk","doi":"10.1016/j.advms.2024.09.002","DOIUrl":"10.1016/j.advms.2024.09.002","url":null,"abstract":"<div><h3>Purpose</h3><div>Inferior vena cava (IVC) involvement by renal cell carcinoma (RCC) is associated with a higher disease stage and is considered a risk factor for poor prognosis. This study aimed to investigate the role of the apparent diffusion coefficient (ADC) of MRI 3D texture analysis in the differentiation of solid and friable tumour thrombus in patients with RCC.</div></div><div><h3>Materials and methods</h3><div>The study involved 27 patients with RCC with tumour thrombus in the renal vein or IVC, surgically treated with nephrectomy and thrombectomy and in whom preoperatively abdominal MRI including the DWI sequence was conducted. For 3D texture analysis, the ADC map was used, and the first-order radiomic features were calculated from the whole volume of the thrombus. All tumour thrombi were histologically classified as solid or friable.</div></div><div><h3>Results</h3><div>The solid and friable thrombus was detected in 51.9 ​% and 48.1 ​% of patients, respectively. No differences in mean values of range, 90th percentile, interquartile range, kurtosis, uniformity and variance were found between groups. Equal sensitivity and specificity (93 ​% and 69 ​%, respectively) of ADC mean, median and entropy in differentiation between solid and friable tumour thrombus, with the highest AUC for entropy (0.808), were observed. Applying the skewness threshold value of 0.09 allowed us to achieve a sensitivity of 86 ​% and a specificity of 92 ​%.</div></div><div><h3>Conclusions</h3><div>In patients with RCC and tumour thrombus in the renal vein or IVC, the 3D texture analysis based on ADC-map allows for precise differentiation of a solid from a friable thrombus.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 434-442"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Spermine Oxidase gene and proinflammatory cytokines expression in gastric cancer patients with and without Helicobacter pylori infection – A pilot study in Polish population","authors":"Magdalena Dzikowiec ,&nbsp;Sandra Galant ,&nbsp;Przemysław Lik ,&nbsp;Katarzyna Góralska ,&nbsp;Dariusz Nejc ,&nbsp;Janusz Piekarski ,&nbsp;Alicja Majos ,&nbsp;Ewa Brzeziańska-Lasota ,&nbsp;Dorota Pastuszak-Lewandoska","doi":"10.1016/j.advms.2024.09.005","DOIUrl":"10.1016/j.advms.2024.09.005","url":null,"abstract":"<div><h3>Purpose</h3><div>Many types of cancer have infectious origins. Gastric cancer patients can demonstrate high seroprevalence of <em>Helicobacter pylori</em> (<em>H. pylori</em>). The aim of the present study was to assess the expression of <em>SMOX</em> gene in the group of Polish patients with gastric cancer. <em>SMOX</em> is believed to promote <em>H. pylori</em>-induced carcinogenesis via inflammation, DNA damage and activation of β-catenin signaling. We also assessed the mRNA expression of selected pro-inflammatory cytokines, i.e. <em>IL-2, IFN-γ, TNF-α</em>, and antimicrobial peptide, cathelicidin.</div></div><div><h3>Materials/methods</h3><div>The study material consisted of gastric tissue samples collected during total gastrectomy from three different places in stomach: from primary tumor, 3 ​cm away from the primary lesion, and from the wall opposite to the primary tumor. After RNA isolation, qPCR reactions were performed for the relevant genes.</div></div><div><h3>Results</h3><div>The obtained results confirmed an increased level of <em>SMOX</em> expression in gastric cancer patients with the history of <em>H. pylori</em> infection. And, as far as we know, this is the first study on <em>SMOX</em> gene expression conducted on tissue taken from a patient, not on a cell line. The levels of pro-inflammatory cytokines, i.e. <em>IL-2, IFN-γ, TNF-α</em>, were also increased, thus indicating their contribution to the specific inflammatory microenvironment of the tumor. Interestingly, the levels of <em>CAMP</em>, encoding antimicrobial peptide, were reduced in all tissue types.</div></div><div><h3>Conclusions</h3><div>The findings confirm that <em>SMOX</em> plays a role in gastric carcinogenesis. However, further research is needed on the role of inflammatory and other factors involved in this process to identify targets for cancer immunotherapy.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 443-450"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}