Advances in medical sciences最新文献

{"title":"Pentraxin 3 as a marker of development and severity of stable coronary artery disease","authors":"Malgorzata Knapp,&nbsp;Monika Gil-Mika,&nbsp;Robert Sawicki,&nbsp;Anna Lisowska,&nbsp;Marcin Kaminski,&nbsp;Bozena Sobkowicz,&nbsp;Katarzyna Ptaszynska","doi":"10.1016/j.advms.2024.07.010","DOIUrl":"10.1016/j.advms.2024.07.010","url":null,"abstract":"<div><h3>Purpose</h3><p>Inflammation plays a crucial role in the development of atherosclerotic plaques. Pentraxin 3 (PTX3) is produced at the site of inflammation and has been identified as a specific marker of atherosclerosis, vascular inflammation, and progression of the coronary artery disease (CAD).</p><p>The aim of the study was to establish if PTX3 has potential relations with classical markers of cardiovascular risk, and if PTX3 may act as an independent risk factor of CAD occurrence and advancement.</p></div><div><h3>Materials and methods</h3><p>The study included 98 patients with stable CAD confirmed in coronary angiography (CAD group) (median age 65 interquartile range [IQR] 61–72 years; 72 ​% men). The control group consisted of 40 patients without CAD.</p></div><div><h3>Results</h3><p>The CAD group had significantly higher PTX3 concentration compared to the control group. There was a correlation with age, male gender, lipid profile and intima-media thickness. There was no correlation between PTX3 concentration and the number of coronary vessels with significant atherosclerotic lesions and the advancement of atherosclerotic lesions on the Gensini scoring scale. The cut-off point was determined for 0.89 ​ng/ml for the exclusion of angiographically significant atherosclerotic lesions.</p></div><div><h3>Conclusions</h3><p>Patients with CAD have significantly higher concentration of PTX3. There was no correlation between PTX3 and the advancement of angiographically significant atherosclerotic lesions in coronary arteries. Low PTX3 concentration may serve as an indicator for the absence of atherosclerosis.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 391-397"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141791647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in hematopoietic stem cell numbers following acute exercise in non-athlete marathon runners","authors":"Özgür Günaştı ,&nbsp;Çiğdem Özdemir ,&nbsp;Kerem T. Özgünen ,&nbsp;Gizem Çiftdal ,&nbsp;Ertuğrul Gezgin ,&nbsp;Selcen Korkmaz Eryılmaz ,&nbsp;Ömer Cumhur Boyraz ,&nbsp;Abdullah Kılcı ,&nbsp;Ümüt Adaş ,&nbsp;Bülent Antmen ,&nbsp;Sanlı Sadi Kurdak","doi":"10.1016/j.advms.2024.09.003","DOIUrl":"10.1016/j.advms.2024.09.003","url":null,"abstract":"<div><h3>Purpose</h3><p>Hematopoietic stem cell (HSC) transplant is one of the curative methods for some patients with hematological malignancies. Granulocyte colony-stimulating factor (G-CSF) is the most common drug used to mobilize CD34⁺ cells, generally found in small numbers. Recent evidence showed that exercise causes transient mobilization in HSC. However, the type and intensity of exercise have not been fully revealed. We aimed to detect a significant increase in stem cell levels following 60 ​min of running at a personalized running pace.</p></div><div><h3>Materials/methods</h3><p>Eighteen runners, 48.2 ​± ​1.9 years with peak oxygen consumption of 46.2 ​± ​1.4 ​ml/kg/min, were enrolled in the study. The cardiopulmonary exercise test was performed to determine the individual running pace, and the participants ran 60-min on a treadmill at an intensity close to their ventilatory threshold (VT). The blood sampling for HSC count was performed before, immediately after, at the 1st, 4th and 24th hour after the 60-min running.</p></div><div><h3>Results</h3><p>The CD34⁺ HSCs were 13.9 ​± ​2.3 ​cells/μl before and significantly increased immediately after to 19.5 ​± ​3.6 ​cells/μl (p ​&lt; ​0.05). The consecutive HSC counts were 15.3 ​± ​2.2, 19.5 ​± ​4.8 and 15.1 ​± ​3.4 ​cells/μl at the 1st, 4th, and 24th hour, respectively.</p></div><div><h3>Conclusion</h3><p>The individual data showed that some runners had higher HSC levels than the transplantation limit before and after the 60-min running trail, which was maintained for 24 ​h. Pre-running high CD34⁺ HSCs may reflect an adaptive response to regular exercise, with a 60-min run near the VT further elevating HSCs. Individualized exercise may be a valuable tool to mobilize the CD34⁺ HSCs in peripheral blood for donors.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 416-420"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Right-ventricle heart failure in PAH vs. HFrEF with secondary PH: Hemodynamic, ergospirometric, and organ function correlations","authors":"Katarzyna Krajewska,&nbsp;Krystian Ejdys,&nbsp;Klaudia Jadczak,&nbsp;Anna Lisowska,&nbsp;Karol A. Kamiński,&nbsp;Bożena Sobkowicz,&nbsp;Katarzyna Ptaszyńska","doi":"10.1016/j.advms.2024.08.003","DOIUrl":"10.1016/j.advms.2024.08.003","url":null,"abstract":"<div><h3>Purpose</h3><div>The goal of the study was to identify markers of organ function used in daily routines that could potentially aid in the overall evaluation of the cardiovascular system in patients with right-ventricle heart failure due to pulmonary arterial hypertension (PAH) and left-ventricle heart failure. We analyzed correlations between parameters from right heart catheterization (RHC), cardiopulmonary exercise test (CPET), and selected laboratory parameters of thyroid, liver, kidneys function and iron homeostasis.</div></div><div><h3>Patients and methods</h3><div>A retrospective analysis included 107 patients (mean age 57.6 ​± ​16.2; 34.6 ​% women), comprising 57 patients with PAH (mean age 54.0 ​± ​18.2; 49.1 ​% women) and 50 patients with heart failure with reduced ejection fraction (HFrEF) ​&lt; ​40 ​% (mean age 61.6 ​± ​12.7; 18 ​% women). All patients underwent CPET. Each patient in the PAH group had RHC performed. Fifteen patients from the HFrEF group underwent RHC, which confirmed the suspicion of pulmonary hypertension (HFrEF-SPH).</div></div><div><h3>Results</h3><div>CPET and laboratory parameters’ analysis showed strong correlations between ventilation/carbon dioxide production (VE/VCO₂) slope and NT-proBNP in HFrEF without secondary PH and HFrEF-SPH groups. In the PAH group, VE/VCO₂ slope correlated with liver and thyroid function but also with morphological parameters of red-cell system.</div><div>Analysis of correlations between laboratory and hemodynamic parameters revealed significant correlations between pulmonary arterial pressure, pulmonary vascular resistance (PVR) and red-cell parameters, especially strong with fT4 in the PAH group.</div></div><div><h3>Conclusions</h3><div>In HFrEF-SPH patients, laboratory parameters strongly correlated with pulmonary pressures and pulmonary capillary wedge pressure (PCWP).</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 421-427"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The vitamin D analog EB1089 sensitizes triple-negative breast cancer cells to the antiproliferative effects of antiestrogens","authors":"Adriana Zárate-Pérez ,&nbsp;Alitzin Pamela Cruz-Cázares ,&nbsp;David Ordaz-Rosado ,&nbsp;Janice García-Quiroz ,&nbsp;Alfonso León-Del-Rio ,&nbsp;Euclides Avila ,&nbsp;Edgar Milo-Rocha ,&nbsp;Lorenza Díaz ,&nbsp;Rocío García-Becerra","doi":"10.1016/j.advms.2024.08.004","DOIUrl":"10.1016/j.advms.2024.08.004","url":null,"abstract":"<div><h3>Purpose</h3><p>Patients bearing estrogen receptor (ER)α-negative breast cancer tumors confront poor prognosis and are typically unresponsive to hormone therapy. Previous studies have shown that calcitriol, the active vitamin D metabolite, can induce ERα expression in ERα-negative cells. EB1089, a calcitriol analog with reduced calcemic effects, exhibits greater potency than calcitriol in inhibiting cancer cell growth. However, the impact of EB1089 on ERα expression in triple-negative breast cancer (TNBC) cells remains unexplored. This study aims to investigate whether EB1089 could induce functional ERα expression in TNBC cell lines, potentially enabling the antiproliferative effects of antiestrogens.</p></div><div><h3>Materials and methods</h3><p>TNBC cell lines HCC1806 and HCC1937 were treated with EB1089, and ERα expression was analyzed using real-time PCR and Western blots. The transcriptional activity of induced ERα was evaluated through a luciferase reporter assay. The antiproliferative effects of tamoxifen and fulvestrant antiestrogens were assessed using the sulforhodamine B assay in the EB1089-treated cells.</p></div><div><h3>Results</h3><p>Our findings indicated that EB1089 significantly induced ERα mRNA and protein expression in TNBC cells. Moreover, EB1089-induced ERα exhibited transcriptional activity and effectively restored the inhibitory effects of antiestrogens, thereby suppressing cell proliferation in TNBC cells.</p></div><div><h3>Conclusion</h3><p>EB1089 induced the expression of functional ERα in TNBC cells, restoring the antiproliferative effects of antiestrogens. These results highlight the potential of using EB1089 as a promising strategy for re-establishment of the antiproliferative effect of antiestrogens as a possible management for TNBC. This research lays the foundation for potential advancements in TNBC treatment, offering new avenues for targeted and effective interventions.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 398-406"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between sleep disorders and clinical outcomes of patients with primary biliary cholangitis","authors":"Shijing Dong ,&nbsp;Simin Zhou ,&nbsp;Jiangpeng Liu ,&nbsp;Nian Chen,&nbsp;Jiwen Li,&nbsp;Zongze Han,&nbsp;Ruiyun Liu,&nbsp;Chenyang Xuan,&nbsp;Weirong Wang,&nbsp;Liping Guo,&nbsp;Lu Zhou","doi":"10.1016/j.advms.2024.08.002","DOIUrl":"10.1016/j.advms.2024.08.002","url":null,"abstract":"<div><h3>Purpose</h3><p>Primary biliary cholangitis (PBC) is a chronic autoimmune liver disease characterized by a range of symptoms, including sleep disturbances. The present study aimed to investigate the prevalence of sleep disorders and the associations between sleep disorders and clinical outcomes in PBC.</p></div><div><h3>Patients and methods</h3><p>We enrolled 177 patients with PBC and 165 healthy controls (age- and sex-matched). Sleep quality was assessed by the Pittsburgh Sleep Quality Index (PSQI). Demographic and clinical data were collected from comprehensive clinical records to investigate whether sleep disorder was correlated with disease severity, therapeutic response and liver cirrhosis.</p></div><div><h3>Results</h3><p>The prevalence of sleep disorders in patients with PBC (50.8 ​%) was significantly higher than healthy controls (18.2 ​%). Patients with sleep disorders presented with higher levels of laboratory parameters including globulin (GLO), aspartate aminotransferase (AST), alkaline phosphatase (ALP), glutamyl transpeptidase (GGT), total bilirubin (TBIL), direct bilirubin (DBIL) and immunoglobulin M (IgM), as well as higher ratio of poor therapeutic response and liver cirrhosis (<em>p</em> ​&lt; ​0.05). There was a positive correlation between global PSQI score and AST, ALP, GGT, TBIL, DBIL and IgM in patients with PBC. Patients with poor therapeutic response and liver cirrhosis in PBC had a higher proportion of sleep disorders and more chaotic sleep patterns, whereas a stronger correlation between sleep quality and laboratory parameters was found in patients with liver cirrhosis.</p></div><div><h3>Conclusions</h3><p>Sleep disorders were prevalent and manifested as adverse effects in PBC. Assessment of sleep quality and intervention were essential to the overall clinical management of patients with PBC.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 385-390"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1896112624000464/pdfft?md5=b0492704685fc4e303759be7a34651df&pid=1-s2.0-S1896112624000464-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentiation of solid and friable tumour thrombus in patients with renal cell carcinoma: The role of MRI apparent diffusion coefficient","authors":"Paweł Kowal ,&nbsp;Krzysztof Ratajczyk ,&nbsp;Wiktor Bursiewicz ,&nbsp;Maciej Trzciniecki ,&nbsp;Karolina Marek-Bukowiec ,&nbsp;Joanna Rogala ,&nbsp;Vasyl Kowalskyi ,&nbsp;Jozef Dragasek ,&nbsp;Andrea Botikova ,&nbsp;Peter Kruzliak ,&nbsp;Yulian Mytsyk","doi":"10.1016/j.advms.2024.09.002","DOIUrl":"10.1016/j.advms.2024.09.002","url":null,"abstract":"<div><h3>Purpose</h3><div>Inferior vena cava (IVC) involvement by renal cell carcinoma (RCC) is associated with a higher disease stage and is considered a risk factor for poor prognosis. This study aimed to investigate the role of the apparent diffusion coefficient (ADC) of MRI 3D texture analysis in the differentiation of solid and friable tumour thrombus in patients with RCC.</div></div><div><h3>Materials and methods</h3><div>The study involved 27 patients with RCC with tumour thrombus in the renal vein or IVC, surgically treated with nephrectomy and thrombectomy and in whom preoperatively abdominal MRI including the DWI sequence was conducted. For 3D texture analysis, the ADC map was used, and the first-order radiomic features were calculated from the whole volume of the thrombus. All tumour thrombi were histologically classified as solid or friable.</div></div><div><h3>Results</h3><div>The solid and friable thrombus was detected in 51.9 ​% and 48.1 ​% of patients, respectively. No differences in mean values of range, 90th percentile, interquartile range, kurtosis, uniformity and variance were found between groups. Equal sensitivity and specificity (93 ​% and 69 ​%, respectively) of ADC mean, median and entropy in differentiation between solid and friable tumour thrombus, with the highest AUC for entropy (0.808), were observed. Applying the skewness threshold value of 0.09 allowed us to achieve a sensitivity of 86 ​% and a specificity of 92 ​%.</div></div><div><h3>Conclusions</h3><div>In patients with RCC and tumour thrombus in the renal vein or IVC, the 3D texture analysis based on ADC-map allows for precise differentiation of a solid from a friable thrombus.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 434-442"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Spermine Oxidase gene and proinflammatory cytokines expression in gastric cancer patients with and without Helicobacter pylori infection – A pilot study in Polish population","authors":"Magdalena Dzikowiec ,&nbsp;Sandra Galant ,&nbsp;Przemysław Lik ,&nbsp;Katarzyna Góralska ,&nbsp;Dariusz Nejc ,&nbsp;Janusz Piekarski ,&nbsp;Alicja Majos ,&nbsp;Ewa Brzeziańska-Lasota ,&nbsp;Dorota Pastuszak-Lewandoska","doi":"10.1016/j.advms.2024.09.005","DOIUrl":"10.1016/j.advms.2024.09.005","url":null,"abstract":"<div><h3>Purpose</h3><div>Many types of cancer have infectious origins. Gastric cancer patients can demonstrate high seroprevalence of <em>Helicobacter pylori</em> (<em>H. pylori</em>). The aim of the present study was to assess the expression of <em>SMOX</em> gene in the group of Polish patients with gastric cancer. <em>SMOX</em> is believed to promote <em>H. pylori</em>-induced carcinogenesis via inflammation, DNA damage and activation of β-catenin signaling. We also assessed the mRNA expression of selected pro-inflammatory cytokines, i.e. <em>IL-2, IFN-γ, TNF-α</em>, and antimicrobial peptide, cathelicidin.</div></div><div><h3>Materials/methods</h3><div>The study material consisted of gastric tissue samples collected during total gastrectomy from three different places in stomach: from primary tumor, 3 ​cm away from the primary lesion, and from the wall opposite to the primary tumor. After RNA isolation, qPCR reactions were performed for the relevant genes.</div></div><div><h3>Results</h3><div>The obtained results confirmed an increased level of <em>SMOX</em> expression in gastric cancer patients with the history of <em>H. pylori</em> infection. And, as far as we know, this is the first study on <em>SMOX</em> gene expression conducted on tissue taken from a patient, not on a cell line. The levels of pro-inflammatory cytokines, i.e. <em>IL-2, IFN-γ, TNF-α</em>, were also increased, thus indicating their contribution to the specific inflammatory microenvironment of the tumor. Interestingly, the levels of <em>CAMP</em>, encoding antimicrobial peptide, were reduced in all tissue types.</div></div><div><h3>Conclusions</h3><div>The findings confirm that <em>SMOX</em> plays a role in gastric carcinogenesis. However, further research is needed on the role of inflammatory and other factors involved in this process to identify targets for cancer immunotherapy.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 443-450"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142278819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution of splenomegaly in liver cirrhosis: Simulation using an electronic circuit","authors":"Jae Cheol Jung,&nbsp;Shin-Young Park,&nbsp;Kyeong Deok Kim,&nbsp;Woo Young Shin,&nbsp;Keon-Young Lee","doi":"10.1016/j.advms.2024.08.001","DOIUrl":"10.1016/j.advms.2024.08.001","url":null,"abstract":"<div><h3>Purpose</h3><p>The evolution of splenomegaly in patients with liver cirrhosis remains largely unknown. In this study, we followed the changes in splenic volume and established the natural course of splenomegaly. We developed an electronic circuit that simulated splenoportal circulation and identified the underlying hemodynamic mechanisms.</p></div><div><h3>Materials and methods</h3><p>This retrospective observational study included 93 patients with cirrhosis. Splenic volumes were measured in imaging studies at 6-month intervals and normalized by the ratio of each patient's maximum volume during follow-up (%Vmax). An electronic simulation model was constructed using software and realized on a breadboard.</p></div><div><h3>Results</h3><p>Overall, the %Vmax increased from 0.77 ​± ​0.21 to a maximum of 1.00 ​± ​0.00 (p ​&lt; ​0.001) during a median follow-up of 23 (3–162) months and then decreased to 0.84 ​± ​0.18 (p ​&lt; ​0.001) during the next 9 (3–132) months. No interventional radiology procedure was performed to improve hepatic fibrosis and portal hypertension. The evolution of %Vmax showed single-peaked symmetry. An electronic simulation model showed that the upslope of the evolution curve was dependent on the increased intrahepatic vascular resistance and portal hypertension, whereas the downslope was dependent on the decreased portosystemic shunt (PSS) resistance.</p></div><div><h3>Conclusions</h3><p>Splenomegaly in cirrhotic patients aggravated over a period of 23 months and then regressed spontaneously to its initial volume. Electronic simulation of splenoportal circulation showed that splenic enlargement was due to the advancement of liver cirrhosis and portal hypertension, whereas its regression was due to the development of a PSS.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 377-384"},"PeriodicalIF":2.5,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1896112624000452/pdfft?md5=be87764a71dd74b373bc2067ecdcc02f&pid=1-s2.0-S1896112624000452-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of nonsurgical periodontal therapy with additional photodynamic therapy on the level of MMP-9 and TIMP-1 in GCF in chronic periodontitis patients-a preliminary pilot study","authors":"Ewa Dolińska ,&nbsp;Anna Skurska ,&nbsp;Małgorzata Pietruska ,&nbsp;Violetta Dymicka-Piekarska ,&nbsp;Robert Milewski ,&nbsp;Anton Sculean","doi":"10.1016/j.advms.2024.07.009","DOIUrl":"10.1016/j.advms.2024.07.009","url":null,"abstract":"<div><h3>Purpose</h3><p>Matrix metalloproteinases (MMPs) catalyze degradation of extracellular matrix proteins. The activity of MMPs is controlled by tissue inhibitors of metalloproteinases (TIMPs). An imbalance in the MMP-9/TIMP-1 ratio has been linked with chronic periodontitis (CP). Photodynamic therapy (PDT) uses visible light, photosensitizer and oxygen to eradicate pathogens. The aim of the study was to evaluate the presence of MMP-9 and TIMP-1 in gingival crevicular fluid (GCF) in chronic periodontitis patients before and after nonsurgical periodontal therapy with additional PDT.</p></div><div><h3>Methods</h3><p>Nineteen patients, each with CP, were included in the study. After periodontal examination one site with a probing depth (PD) ​≥ ​4 ​mm was selected. The patients received scaling and root planing (SRP) with additional PDT by means of HELBO® diode minilaser. Prior to treatment, and after 3 and 6 months, the following parameters were estimated from the same site: PD, gingival recession (GR), clinical attachment level (CAL), plaque index (PI), bleeding on probing (BOP) and sulcus fluid flow rate (SFFR). The levels of MMP-9 and TIMP-1 in GCF were determined.</p></div><div><h3>Results</h3><p>Compared to baseline, the levels of MMP-9 and TIMP-1 did not show statistically significant differences after 3 and 6 months. According to Spearman's rank correlations, MMP-9 was positively correlated with SFFR at all time points. PD, CAL and PI showed a statistically significant decrease compared to baseline (p ​&lt; ​0.001). SFFR decreased but not significantly.</p></div><div><h3>Conclusion</h3><p>Nonsurgical periodontal therapy in conjunction with PDT was clinically effective but it had no effect on the levels of MMP-9 and TIMP-1 in GCF.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 362-367"},"PeriodicalIF":2.5,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1896112624000439/pdfft?md5=90f50aed205014667e68e158055748e7&pid=1-s2.0-S1896112624000439-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141764790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intestinal fructose transporters GLUT5 and GLUT2 in children and adolescents with obesity and metabolic disorders","authors":"Anna Socha-Banasiak ,&nbsp;Agata Sakowicz ,&nbsp;Zuzanna Gaj ,&nbsp;Michał Kolejwa ,&nbsp;Agnieszka Gach ,&nbsp;Elżbieta Czkwianianc","doi":"10.1016/j.advms.2024.07.008","DOIUrl":"10.1016/j.advms.2024.07.008","url":null,"abstract":"<div><h3>Purpose</h3><p>The excessive fructose intake including high-fructose corn syrup (HFCS) may be responsible for increase of obesity occurrence. This study was designed to find potential differences in duodenal fructose transporters on mRNA and protein levels between obese and normal weight children and adolescents.</p></div><div><h3>Materials/methods</h3><p>We performed a cross-sectional study on a group of 106 hospitalized patients aged 12 to 18. Glucose transporter 2 (<em>GLUT2</em>) and glucose transporter 5 (<em>GLUT5</em>) mRNA as well as protein levels (ELISA and Western blot methods) were assessed in duodenal mucosa biopsies of the patients categorized as obese or normal weight. Additionally, the expression of the aforementioned transporters was analyzed in patients based on the presence of insulin resistance (IR) and metabolic syndrome (MS).</p></div><div><h3>Results</h3><p>In children with obesity, increased duodenal protein levels of GLUT5 (Relative protein GLUT5 expression/ACTB) (0.027 ​± ​0.009 vs. 0.011 ​± ​0.006, p ​&lt; ​0.05) but not GLUT2 as compared with the normal weight group, were revealed. No significant differences in duodenal relative <em>GLUT2</em> and <em>GLUT5</em> genes expression between the studied groups were found. There was no relationship between the presence of IR or MS and intestinal mRNA <em>GLUT2</em> and <em>GLUT5</em> as well as GLUT2 protein expression.</p></div><div><h3>Conclusion</h3><p>The upregulation of the duodenal GLUT5 may contribute to obesity occurrence in children and adolescents.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"69 2","pages":"Pages 349-355"},"PeriodicalIF":2.5,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141764791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}