Advances in medical sciences最新文献

{"title":"Investigation of the effects of umbilical cord and adipose-derived mesenchymal stem cells on endoplasmic reticulum stress in cadmium-induced rat kidney","authors":"Bahar Kartal ,&nbsp;Uygar Saçik ,&nbsp;Güven Erbil","doi":"10.1016/j.advms.2025.07.002","DOIUrl":"10.1016/j.advms.2025.07.002","url":null,"abstract":"<div><h3>Purpose</h3><div>The hazardous heavy metal cadmium (Cd) has the potential to cause long-term kidney damage, mostly dependent on autophagy. Endoplasmic reticulum (ER) stress has been recognized as a primary source of Cd-induced toxicity. The ER chaperone GRP78 binds ER stress sensors, keeping them dormant. Exposure to Cd increases ER stress, a well-known inducer of autophagy. Adipose-derived mesenchymal stem cells (AD-MSC) are potentially useful tissue engineering and cellular treatment tools. Various disorders are treated with human umbilical cord MSCs (HUC-MSCs). They possess several unique qualities that are necessary for their therapeutic uses. The study aimed to investigate the effects of AD-MSCs and HUC-MSCs on Cd-induced nephrotoxicity.</div></div><div><h3>Methods</h3><div>The study used 36 male Wistar albino rats that were divided into six groups: control, AD-MSC, HUC-MSC, Cd, Cd ​+ ​AD-MSC, and Cd ​+ ​HUC-MSC. Hematoxylin and eosin (H&amp;E) were used to stain the renal tissues in preparation for a histological analysis. Furthermore, the ER stress level was assessed by measuring GRP78 immunoexpression. Additionally, LC3B and Beclin-1 immunostaining were used to determine the autophagy.</div></div><div><h3>Results</h3><div>The histopathological results showed that the glomerular structure, proximal and distal tubules were disrupted in rat kidneys from the Cd group. Treatment with AD-MSCs and HUC-MSCs restored renal histological damage caused by Cd. Additionally, in Cd-induced renal tissues, there was an increase in the immunoexpression of the autophagic sensors LC3B and Beclin-1 and the ER stress indicator GRP78.</div></div><div><h3>Conclusion</h3><div>MSCs enabled Cd-damaged kidney tissues to regain an almost healthy histological structure.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 284-290"},"PeriodicalIF":2.6,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"QT variability and myocardial repolarization in sleep apnea: implications for cardiac risk","authors":"Aleksandra Jarecka-Dobroń ,&nbsp;Wojciech Braksator ,&nbsp;Paweł Chrom","doi":"10.1016/j.advms.2025.07.004","DOIUrl":"10.1016/j.advms.2025.07.004","url":null,"abstract":"<div><h3>Purpose</h3><div>Due to the increased risk of sudden cardiac death, we decided to assess ECG parameters related to the stability of the myocardial repolarization period, i.e. the corrected QT interval (QTc) and derivatives describing its variability (QTV and QTVi).</div></div><div><h3>Methods</h3><div>Healthy volunteers (n ​= ​187) with visceral obesity, aged 35–65 years, were included. Each participant underwent Holter-ECG and polygraphy simultaneously. According to the severity of breathing disorders during sleep the cohort was divided into 3 groups: Respiratory Event Index (REI) 5–14/hour, REI 15–30/hour, REI ≥ 30/hour. The values of QT parameters were compared between obstructive sleep apnea (OSA)-positive and -negative group as well as among OSA positive group (depending on the OSA severity degree).</div></div><div><h3>Results</h3><div>We enrolled 121 patients, mean age 47.57 ​± ​9.36 (47 ​% female), mean BMI 32.18 ​± ​5.98 ​kg/m², 70 (58 ​%) of them were diagnosed with OSA, mean REI 26.79 ​± ​25.66/hour. In OSA group, QTV and QTVi were higher (p ​&lt; ​0.001) however QTc max was not significantly longer (p ​= ​0.06).</div><div>Furthermore, we found significantly increased QTc max and QTVi in OSA positive patients during respiratory events compared to normal breathing (p ​= ​0.02 and p ​= ​0.008, respectively). Additionally, we found a positive correlation between REI and QTc max (p ​= ​0.004, R ​= ​0.22). Parameters related to hypoxia (oxygen desaturation index 4 ​%, time with SpO2 &lt;90 ​%, SpO2 min, SpO2 mean) also presented a positive correlation with QTc max. Variables were not dependent on age or BMI.</div></div><div><h3>Conclusions</h3><div>Repolarization of cardiomyocytes is impaired in patients with OSA. The severity of impairment is positively correlated with the severity of sleep-related breathing disorders and hypoxemia.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 301-307"},"PeriodicalIF":2.6,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of hepatitis D virus in chronic hepatitis B patients: findings from Poland","authors":"Dorota Zarębska-Michaluk ,&nbsp;Michał Brzdęk ,&nbsp;Krystyna Dobrowolska ,&nbsp;Diana Martonik ,&nbsp;Anna Parfieniuk-Kowerda ,&nbsp;Jakub Janczura ,&nbsp;Kinga Brzdęk ,&nbsp;Robert Pleśniak ,&nbsp;Agnes Piszcz ,&nbsp;Robert Flisiak","doi":"10.1016/j.advms.2025.07.003","DOIUrl":"10.1016/j.advms.2025.07.003","url":null,"abstract":"<div><h3>Purpose</h3><div>This study aimed to determine the prevalence of hepatitis B/hepatitis D virus (HBV/HDV) co-infections in eastern Poland.</div></div><div><h3>Materials/methods</h3><div>We included consecutive patients with chronic hepatitis B treated with nucleos(t)ide analogues (NA) in the study, in whom we performed anti-HDV assays verified by molecular testing.</div></div><div><h3>Results</h3><div>The analysed population consisted of 398 patients, predominantly male with a median age of 50 years, 58.3 ​% of whom had comorbidities. Forty-three patients (10.8 ​%) were diagnosed with cirrhosis. Patients were treated with entecavir, tenofovir, or lamivudine for a median of 5 years. At the start of treatment, the median viral load was 5110 IU/ml, and 98 ​% of patients achieved viral eradication during the therapy. In the study population, anti-HDV antibodies were detected in 6 patients (1.5 ​%), 3 men and 3 women, of whom in 1 (0.25 ​%) the presence of genetic material of the HDV was confirmed by molecular testing.</div><div>Among anti-HDV-positive patients, 3 (50 ​%) had cirrhosis, 1 of them underwent liver transplantation, and 2 had extrahepatic malignancy. All of them were negative for hepatitis B virus envelope (HBe) antigen and had antibodies to HBe antigen present at the beginning of antiviral treatment. All anti-HDV-positive patients achieved viral clearance during the therapy.</div></div><div><h3>Conclusion</h3><div>In a population of almost four hundred patients infected with HBV, we observed a low prevalence of anti-HDV antibodies of 1.5 ​% and HDV replication of only 0.25 ​%. Half of the 6 patients with serologic evidence of co-infection had cirrhosis, so HDV likely played a role in disease progression.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 277-283"},"PeriodicalIF":2.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144722652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of anti-HLA antibodies on the immunological status of patients with cardiac allograft vasculopathy (CAV) after heart transplantation.","authors":"Katarzyna Kowalik, Joanna Was, Katarzyna Kozar-Kaminska, Ilona Minota, Krzysztof Komuda, Aneta Rekawek, Tomasz Zielinski","doi":"10.1016/j.advms.2025.07.001","DOIUrl":"10.1016/j.advms.2025.07.001","url":null,"abstract":"<p><strong>Purpose: </strong>Heart transplantation (HTx) is an established method of treating patients with end-stage heart failure. Properly selected immunosuppression effectively reduces the risk of acute transplant rejection; however, it may also lead to various side effects including cardiac allograft vasculopathy (CAV), which is one of the most common causes of death in patients during the first year after HTx. There is an urgent need to develop new strategies for monitoring a patient's immune system to modify the immunosuppressive therapy, if needed. The purpose of this study is to compare the immune status of patients with diagnosed CAV ​+ ​to that of patients without CAV-.</p><p><strong>Material and methods: </strong>We collected blood samples from patients long-term after transplantation: 19 clinically stable patients CAV+ and 19 CAV-. Immunological parameters were assessed by analyzing lymphocyte subpopulations with flow cytometry and detecting the presence of alloantibodies with the use of Luminex technology.</p><p><strong>Results: </strong>Statistical analysis demonstrated significant differences between CAV+ and CAV- patients only within the group of patients with detected alloantibodies (AlloAb+). The most notable observation is that a significantly higher proportion of CAV ​+ ​AlloAb ​+ ​patients demonstrated the CD4⁺ T lymphocyte population expressing IL2 compared to CAV-AlloAb ​+ ​patients. Similarly, in these groups, we observed a prevalence of CD8⁺ lymphocytes expressing IFNγ+ and IL2+TNFα+IFNγ+.</p><p><strong>Conclusion: </strong>Our study provides new evidence that the presence of alloantibodies in the bloodstream of patients after HTx impacts T cells activation and subsequent cytokine production. These data may facilitate establishing parameters that could help identify patients with a higher risk of CAV progression.</p>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":" ","pages":"291-300"},"PeriodicalIF":2.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Restoring impaired osteogenic differentiation of diabetic rat stromal cells using epigenetic inhibitors","authors":"Mahshid Hodjat ,&nbsp;Fazlullah Khan ,&nbsp;Hadiseh Mohammadpour ,&nbsp;Nasrin Asadi","doi":"10.1016/j.advms.2025.06.001","DOIUrl":"10.1016/j.advms.2025.06.001","url":null,"abstract":"<div><h3>Purpose</h3><div>Epigenetic regulation plays a crucial role in gene expression and is recognized as a key contributor to diabetes-related complications. This study explores the osteogenic differentiation potential of stem cells isolated from the periodontal ligament (PDL) and bone marrow (BM) of diabetic rats. It investigates the effects of DNA methyltransferase and histone deacetylase inhibitors on the differentiation capacity of diabetic stem cells, searching for underlying mechanisms.</div></div><div><h3>Method</h3><div>Diabetes was induced in 5-week-old male Wistar rats using streptozotocin (STZ). Bone parameters were assessed via micro-CT, and stem cells isolated from mandibles and femurs were treated with 5-azacytidine or Trichostatin A in osteogenic medium. Osteogenic differentiation was evaluated through alkaline phosphatase (ALP) activity, Alizarin Red staining, and mRNA expression of osteogenic markers using real-time PCR.</div></div><div><h3>Results</h3><div>A significant decrease in total BMD and BV/TV of the femur and mandible was observed in STZ-induced diabetic rats compared to control. Cells isolated from diabetic PDL and BM showed impaired mineralization capacity and downregulated osteogenic markers. Treatment with Trichostatin A or 5-azacytidine restored mineralization potential, increased ALP activity, and upregulated the expression of <em>RUNX2</em> and β-catenin.</div></div><div><h3>Conclusion</h3><div>Our results revealed the underlying epigenetic mechanisms responsible for the impaired osteogenic differentiation capacity of stem cells in diabetes. These findings highlight the potential of epigenetic modulators to restore stem cell function and enhance bone regeneration. This approach holds promise for improving diabetes-related skeletal complications and advancing tissue engineering strategies, including the development of scaffold-based therapies for fracture repair, periodontal regeneration, and implant integration in diabetic patients.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 266-276"},"PeriodicalIF":2.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic instability at the PTEN locus and altered miRNA-21 and miRNA-200a expression in gastric cancer patients in Poland","authors":"Magdalena Dzikowiec ,&nbsp;Sandra Galant ,&nbsp;Przemysław Lik ,&nbsp;Monika Migdalska-Sęk ,&nbsp;Dariusz Nejc ,&nbsp;Janusz Piekarski ,&nbsp;Alicja Majos ,&nbsp;Ewa Brzeziańska-Lasota ,&nbsp;Dorota Pastuszak-Lewandoska","doi":"10.1016/j.advms.2025.05.003","DOIUrl":"10.1016/j.advms.2025.05.003","url":null,"abstract":"<div><h3>Purpose</h3><div>Gastric cancer is often diagnosed late, and is associated with poor long-term prognosis. The aim of the study was to look for non-invasive potential biomarkers involved in gastric carcinogenesis, with diagnostic or prognostic significance.</div></div><div><h3>Material/methods</h3><div>Gastric tissue samples, from three different regions of the stomach, including the primary tumor, macroscopically unchanged gastric tissues, as well as serum, were collected from patients diagnosed with gastric cancer. Serum samples were also obtained from a control group. The analyzed parameters were: expression levels of <em>PTEN</em>, miRNA-21 and miRNA-200a using qPCR method and the frequency of LOH/MSI at the <em>PTEN locus</em> using four microsatellite markers.</div></div><div><h3>Results</h3><div>The obtained results revealed significantly decreased expression of <em>PTEN</em> in gastric tumor tissue and statistically significant differences between the studied tissue samples from different stomach regions. <em>PTEN</em> expression in patients with LOH/MSI was decreased two-fold compared to patients without genetic instability, indicating a potential mechanism of gene silencing. Another mechanism of <em>PTEN</em> silencing could be due to miRNA activity: significant negative correlations were found between <em>PTEN</em> and the studied miRNAs expression levels. In serum, miRNA-21 expression was increased in the group of patients, while miRNA-200a expression was decreased, and the differences were statistically significant compared to controls. Receiver operating characteristic curve analysis for miRNA-200a revealed 92 ​% sensitivity and 77 ​% specificity.</div></div><div><h3>Conclusions</h3><div>The obtained results suggest that miRNA-21 and miRNA-200a could be considered as diagnostic biomarkers differentiating patients with gastric cancer from healthy individuals; however, it should be verified on a larger group of patients.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 248-254"},"PeriodicalIF":2.5,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of bone marrow-derived very small embryonic-like stem cells followed by thrombin-activated platelet-rich plasma for endometrial regeneration","authors":"Jeevitaa Kshersagar ,&nbsp;Akshay A. Kawale ,&nbsp;Leena R. Chaudhari ,&nbsp;Mrunal N. Damle ,&nbsp;Rakesh Kumar Sharma ,&nbsp;Meghnad G. Joshi","doi":"10.1016/j.advms.2025.05.002","DOIUrl":"10.1016/j.advms.2025.05.002","url":null,"abstract":"<div><h3>Purpose</h3><div>Endometrium, a dynamic tissue undergoing cyclic changes, plays a pivotal role in reproductive health. Disruptions in its structure and function can lead to infertility and pregnancy complications. Stem cell-based therapies, including very small embryonic-like stem cells (VSELS) and platelet-rich plasma (PRP), have shown promise in tissue regeneration.</div></div><div><h3>Methods</h3><div>We investigated the efficacy of bone marrow-derived VSELS combined with thrombin-activated PRP (aPRP) for endometrial regeneration in a murine model of disturbed endometrium (DE). Characterization of bone marrow very small embryonic like stem cells (BM VSELS) revealed pluripotency markers and negative expression for CD34, Tie-2, Thy, CD133, CD90, and delta-like protein (DLK).</div></div><div><h3>Results</h3><div>Transplantation of BM VSELS-aPRP resulted in their engraftment in the endometrium, with enhanced endometrial thickness, collagen reformation, and improved marker expression compared to controls. Immunohistochemical analysis demonstrated increased expression of α-SMA, CK-18, CK-19, E-Cad, Cla-1, CX-40, and ZO-1 in the transplant group. Pregnancy outcomes improved in the BM VSELS-aPRP group, with successful conception and delivery of healthy pups.</div></div><div><h3>Conclusion</h3><div>This study highlights the regenerative potential of BM VSELS-aPRP for endometrial repair and suggests a novel therapeutic approach for endometrial disorders and infertility.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 255-265"},"PeriodicalIF":2.5,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meconium ferritin amounts and birth size of neonates: a pilot study","authors":"Ewa Skarżyńska ,&nbsp;Artur Jakimiuk ,&nbsp;Tadeusz Issat ,&nbsp;Krzysztof Krasuski ,&nbsp;Barbara Lisowska-Myjak","doi":"10.1016/j.advms.2025.05.001","DOIUrl":"10.1016/j.advms.2025.05.001","url":null,"abstract":"<div><h3>Purpose</h3><div>Ferritin amounts that accumulate in the meconium may provide new postnatal insights into intrauterine iron homeostasis and neonatal preparedness for the postnatal period. The most dynamic increases in fetal iron stores and fetal growth occur during the third trimester.</div></div><div><h3>Materials and methods</h3><div>This study involved 122 neonates born between 36 and 41 weeks of gestation, with birth weights from 2650 ​g to 4960 ​g and birth lengths ranging from 50 ​cm to 60 ​cm. Ferritin amounts per gram of meconium were determined via ELISA in the first meconium passed after birth.</div></div><div><h3>Results</h3><div>A significant week-by-week increase in the birth weight and length (<em>p</em> ​&lt; ​0.05) was accompanied by decreasing meconium ferritin amounts (<em>p</em> ​= ​0.021) across the gestational age range of 36–41 weeks. There were negative correlations (p ​&lt; ​0.05) between the systematic decrease in meconium ferritin amounts and the gestational age across the same range (r ​= ​-0.18) and between ferritin amounts and the birth weight and length of newborns (r ​= ​-0.20 and r ​= ​-0.31). Neonates born at 36–37 weeks of gestation had lower birth weight and length, while their meconium ferritin amounts were nearly twice as high as in neonates born at 38–39 weeks or 40–41 weeks (<em>p</em> ​&lt; ​0.05).</div></div><div><h3>Conclusions</h3><div>Systematic decreases in meconium ferritin amounts from 36 to 41 weeks of gestation may suggest a gradual and gestational age-appropriate maturation of the mechanisms responsible for adaptation of the fetus to postnatal life. Determining a cut-off value for meconium ferritin amounts could aid in optimal management of newborns after birth.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 237-242"},"PeriodicalIF":2.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of systemic immune–inflammation index with in-hospital mortality of cardiogenic shock patients supported with extracorporeal membrane oxygenation","authors":"Shixing Li ,&nbsp;Hao Wang ,&nbsp;Jin Yu,&nbsp;Jingsong Xu,&nbsp;Yan Xu","doi":"10.1016/j.advms.2025.04.002","DOIUrl":"10.1016/j.advms.2025.04.002","url":null,"abstract":"<div><h3>Purpose</h3><div>The systemic immune-inflammation index (SII) is a useful predictor in cardiovascular diseases. The purpose of this study was to investigate the association of SII with in–hospital mortality in patients with cardiogenic shock (CS) supported with extracorporeal membrane oxygenation (ECMO).</div></div><div><h3>Patients and Methods</h3><div>A total of 126 CS patients received ECMO implantation between January 2020 and December 2023. SII was calculated as follows: SII ​= ​neutrophil count × platelet count / lymphocyte count at admission. Participants were divided into high or low SII group based on the cut–off value of SII. In–hospital mortality was compared between the groups.</div></div><div><h3>Results</h3><div>The optimal SII cut–off value for predicting in–hospital mortality in CS patients supported with ECMO was 1735.9 (AUC 0.68, <em>p</em> ​= ​0.001). In–hospital mortality was significantly higher in the high SII group compared to the low SII group (59.09 % vs. 21.67 %, <em>p</em> &lt;0.001). The univariate and multivariate logistic regression analyses had shown that SII and left ventricular ejection fraction (LVEF) were identified as independent predictors of in–hospital mortality in CS patients supported with ECMO (OR: 1.001, 95 % CI: 1.000–1.002, <em>p</em> ​= ​0.007 and OR: 0.881, 95 % CI: 0.803–0.966, <em>p</em> ​= ​0.007, respectively). SII combined with LVEF offered a superior prognostic capability compared to SII alone (AUC 0.707, v. s. AUC 0.68).</div></div><div><h3>Conclusion</h3><div>We demonstrated that elevated admission SII was independently associated with in-hospital mortality in CS patients supported with ECMO. These findings highlight the potential role of SII as an indicator of mortality risk in this population.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 243-247"},"PeriodicalIF":2.5,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collagen (rs3134646) and ARHGAP15 (rs4662344) genetic variants may predispose to colonic diverticulosis","authors":"Piotr Nehring ,&nbsp;Grzegorz Placha ,&nbsp;Adam Przybyłkowski","doi":"10.1016/j.advms.2025.04.001","DOIUrl":"10.1016/j.advms.2025.04.001","url":null,"abstract":"<div><h3>Purpose</h3><div>Colonic diverticulosis is a common condition in older adults in the entire world. Besides environmental factors, there are emerging data on underlying genetic predisposition to diverticula formation. The study aimed to identify genetic factors associated with colonic diverticulosis.</div></div><div><h3>Patients and methods</h3><div>The study involved 323 patients, 134 with colonic diverticulosis, and 189 healthy controls. In all study participants the following genetic variants were assessed using RT-PCR: rs1800587 in <em>IL1</em>, rs16944, and rs2853550 in <em>IL1B</em>, rs3134646, and rs6434304 in <em>COL3A1</em>, rs3771810, and rs3771863 in <em>TACR1</em>, rs4644560 in <em>NK2R</em>, rs4662344 in <em>ARHGAP15</em>, rs67153654 in <em>FAM155A</em>, and rs7848647 in <em>TNFSF15</em>.</div></div><div><h3>Results</h3><div>Both, the allele C of the <em>COL3A1</em> (rs3134646) variant and allele T of the <em>ARHGAP15</em> (rs4662344) variant, were shown to be associated with diverticulosis, compared to healthy controls in co-dominant and recessive models. Both of them were more frequently reported in patients with colonic diverticulosis, compared to healthy controls.</div><div>There were no allelic or genetic associations in patients with diverticulosis or diverticulitis compared to healthy controls, for the following genetic variants: <em>IL1A</em> (rs1800587), <em>IL1B</em> (rs16944, rs2853550), <em>COL3A1</em> (rs6434304), <em>TACR1</em> (rs3771810, rs3771863), <em>NK2R</em> (rs4644560), <em>FAM155A</em> (rs67153654) and <em>TNFSF15</em> (rs7848647).</div></div><div><h3>Conclusions</h3><div>The allele C of <em>COL3A1</em> (rs3134646) and allele T of the <em>ARHGAP15</em> (rs4662344) may predispose to colonic diverticulosis. When viewed in the context of previous studies, these findings suggest that the development of colonic diverticula may be influenced by the involvement and degradation of the extracellular matrix.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 231-236"},"PeriodicalIF":2.5,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}