iMeta最新文献

{"title":"Single-microbe RNA sequencing uncovers unexplored specialized metabolic functions of keystone species in the human gut","authors":"Yifei Shen,&nbsp;Wenxin Qu,&nbsp;Mengdi Song,&nbsp;Tianyu Zhang,&nbsp;Chang Liu,&nbsp;Xiaofeng Shi,&nbsp;Xinxin Xu,&nbsp;Jingjing Jiang,&nbsp;Liguo Ding,&nbsp;Fangyu Mo,&nbsp;Zheying Mao,&nbsp;Mingzhu Huang,&nbsp;Ziye Xu,&nbsp;Jiaye Chen,&nbsp;Enhui Shen,&nbsp;Jian Ruan,&nbsp;Jiong Liu,&nbsp;Michael P. Timko,&nbsp;Yu Chen,&nbsp;Longjiang Fan,&nbsp;Shufa Zheng,&nbsp;Yongcheng Wang","doi":"10.1002/imt2.70035","DOIUrl":"https://doi.org/10.1002/imt2.70035","url":null,"abstract":"<p>The human body is inhabited by trillions of microorganisms that play a crucial role in health and diseases. Our understanding of the species and functional composition of the human gut microbiome is rapidly expanding, but it is still mainly based on taxonomic profiles or gene abundance measurements. As such, little is known about the species–function heterogeneity and dynamic activities in human microecosystem niches. By applying a novel gut-specific single-microbe ribonucleic acid (RNA) sequencing and analytical framework on three healthy donors with distinct enterotypes, we created a comprehensive transcriptional landscape of the human gut microbiome and dissected functional specialization in 38,922 single microbes across 198 species. We investigated the functional redundancy and complementarity involved in short-chain fatty acids related central carbon metabolism and studied the heterogeneity and covariation of single-microbe metabolic capacity. Comparing the human gut microbiome at different times throughout the day, we were able to map diurnal dynamic activities of the gut microbiome and discovered its association with sub-population functional heterogeneous. Remarkably, using single-microbe RNA sequencing, we systematically dissected the metabolic function heterogeneity of <i>Megamonas funiformis</i>, a keystone species in Asian populations. Together with in vitro and in vivo experimental validations, we proved <i>M. funiformis</i> can effectively improve mineral absorption through exogenous phytic acid degradation, which could potentially serve as a probiotic that reduces malnutrition caused by deficiency of mineral elements. Our results indicated that species-function heterogeneity widely exists and plays important roles in the human gut microbiome, and through single-microbe RNA sequencing, we have been able to capture the transcriptional activity variances and identify keystone species with specialized metabolic functions of possible biological and clinical importance.</p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"4 3","pages":""},"PeriodicalIF":23.7,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.70035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging core enzyme structures for microbiota targeted functional regulation: Urease as an example","authors":"Shengguo Zhao,&nbsp;Huiyue Zhong,&nbsp;Yue He,&nbsp;Xiaojiao Li,&nbsp;Li Zhu,&nbsp;Zhanbo Xiong,&nbsp;Xiaoyin Zhang,&nbsp;Nan Zheng,&nbsp;Diego P. Morgavi,&nbsp;Jiaqi Wang","doi":"10.1002/imt2.70032","DOIUrl":"https://doi.org/10.1002/imt2.70032","url":null,"abstract":"<p>Microbial communities play critical roles in various ecosystems. Despite extensive research on the taxonomic and functional diversity of microbial communities, effective approaches to regulate targeted microbial functions remain limited. Here, we present an innovative methodology that integrates core enzyme identification, protein structural characterization, regulator virtual screening, and functional validation to achieve precise microbiome functional regulation. As a proof of concept, we focused on the regulation of urea decomposition by the rumen microbiota in ruminants. Through metagenomic analysis, we identified the core urease gene and its corresponding microbial genome (MAG257) affiliated with the unclassified Succinivibrionaceae, and reconstructed its complete gene cluster. Structural analysis of the urease catalytic subunit (UreC) via cryo-electron microscopy (cryo-EM) revealed detailed features of its active site, guiding molecular docking studies that identified epiberberine, a natural compound with potent urease inhibitory activity. Validation in a rumen simulation system demonstrated that epiberberine significantly reduced urea decomposition and enhanced nitrogen utilization. This study establishes a robust framework that combines structural biology and computational screening to achieve targeted microbiome functional regulation, offering a promising tool for microbiome engineering and broader applications in animal productivity, human health, environmental improvement, and biotechnology.</p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"4 3","pages":""},"PeriodicalIF":23.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.70032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agriculture increases potential health risks of vertebrate viruses in soils","authors":"Kankan Zhao,&nbsp;Yiling Wang,&nbsp;Ran Xue,&nbsp;Xingmei Liu,&nbsp;Bin Ma,&nbsp;Jianming Xu","doi":"10.1002/imt2.70034","DOIUrl":"https://doi.org/10.1002/imt2.70034","url":null,"abstract":"<p>Here, we conducted a large-scale investigation of vertebrate viruses in soils and found soil was a mediator of vertebrate viruses. Compared to natural soils, agricultural soils possessed distinct prevalence patterns, with a higher detection rate and richness for vertebrate viruses and higher potential health risks.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"4 3","pages":""},"PeriodicalIF":23.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.70034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and biomarker analysis of neoadjuvant disitamab vedotin (RC48-ADC) combined immunotherapy in patients with muscle-invasive bladder cancer: A multi-center real-world study","authors":"Jiao Hu,&nbsp;Luzhe Yan,&nbsp;Jinhui Liu,&nbsp;Minfeng Chen,&nbsp;Peihua Liu,&nbsp;Dingshan Deng,&nbsp;Chaobin Zhang,&nbsp;Yunbo He,&nbsp;Benyi Fan,&nbsp;Huihuang Li,&nbsp;Guanghui Gong,&nbsp;Jiatong Xiao,&nbsp;Ruizhe Wang,&nbsp;Xiao Guan,&nbsp;Shiyu Tong,&nbsp;Yangle Li,&nbsp;Nannan Li,&nbsp;Zhiwang Tang,&nbsp;Teng Zhang,&nbsp;Hao Li,&nbsp;Bin Huang,&nbsp;Ning Gao,&nbsp;Wei He,&nbsp;Zhiyong Cai,&nbsp;Yifan Liu,&nbsp;Zefu Liu,&nbsp;Yu Gan,&nbsp;Yu Cui,&nbsp;Yuanqing Dai,&nbsp;Yi Cai,&nbsp;Zhenyu Nie,&nbsp;Zhenyu Ou,&nbsp;Jinbo Chen,&nbsp;Xiongbing Zu","doi":"10.1002/imt2.70033","DOIUrl":"https://doi.org/10.1002/imt2.70033","url":null,"abstract":"<p>In this study, 102 cisplatin-ineligible patients with muscle-invasive bladder cancer (MIBC) who received neoadjuvant RC48-ADC combined with immunotherapy were included. We evaluated the pathological responses and explored multiple clinical characteristics to identify independent predictive indicators of the efficacy. The results showed that neoadjuvant RC48-ADC combined with immunotherapy had promising efficacy. Furthermore, we collected 11 MIBC samples and performed single-cell RNA sequencing. All BLCA epithelial cells were identified as four subclusters. We conducted differential gene expression/functional enrichment analysis, cell proportion analysis, cell cycle analysis, CNV analysis, and pseudotemporal analysis on all tumor cells to evaluate potential efficacy-predictive biomarkers and the evolutionary patterns of tumor cells during neoadjuvant treatment. The results indicated that the combined detection of HER2 and HSPA1A expression in C3 subcluster based on single-cell RNA sequencing is a potential strategy for predicting efficacy. In addition, C3 plays a dominant role in the emergence of drug-resistance during the evolution of BLCA epithelial cells.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"4 3","pages":""},"PeriodicalIF":23.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.70033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing human gut health: Global innovations in dysbiosis management","authors":"Reda El Boukhari,&nbsp;Maima Matin,&nbsp;Latifa Bouissane,&nbsp;Michał Ławiński,&nbsp;Oleh Lushchak,&nbsp;Rajeev K. Singla,&nbsp;Michel-Edwar Mickael,&nbsp;Jordi Mayneris-Perxachs,&nbsp;Maria Eleni Grafakou,&nbsp;Shuhua Xu,&nbsp;Bowen Liu,&nbsp;Jiayi Guan,&nbsp;Andrzej Półtorak,&nbsp;Arkadiusz Szpicer,&nbsp;Agnieszka Wierzbicka,&nbsp;Nikolay T. Tzvetkov,&nbsp;Maciej Banach,&nbsp;Jarosław Olav Horbańczuk,&nbsp;Artur Jóźwik,&nbsp;Marco Cascella,&nbsp;Bairong Shen,&nbsp;Vasil Radoslavov Pirgozliev,&nbsp;Dongdong Wang,&nbsp;Olena Litvinova,&nbsp;Olga Adamska,&nbsp;Agnieszka Kamińska,&nbsp;Marcin Łapiński,&nbsp;Artur Stolarczyk,&nbsp;Ioana Berindan-Neagoe,&nbsp;Luigi Milella,&nbsp;Andy Wai Kan Yeung,&nbsp;Prashanth Suravajhala,&nbsp;Anupam Bishayee,&nbsp;Ronan Lordan,&nbsp;Laszlo Barna Iantovics,&nbsp;Ricardo Lagoa,&nbsp;Monika Michalczuk,&nbsp;Jivko Stoyanov,&nbsp;A. Douglas Kinghorn,&nbsp;Banaz Jalil,&nbsp;Wolfram Weckwerth,&nbsp;Bey Hing Goh,&nbsp;Meng-Yao Li,&nbsp;Gyaneshwer Chaubey,&nbsp;Gian Luigi Russo,&nbsp;Sara Frazzini,&nbsp;Luciana Rossi,&nbsp;Maurizio Battino,&nbsp;Wei Jia,&nbsp;Qi Su,&nbsp;Xiaoqiang Ma,&nbsp;Judith M. Rollinger,&nbsp;Simon K.-M. R. Rittmann,&nbsp;Helen Sheridan,&nbsp;John J. Walsh,&nbsp;Gérard Lizard,&nbsp;Tomasz M. Karpiński,&nbsp;Ana Sanches Silva,&nbsp;Jakub Piwowarski,&nbsp;Liwei Xie,&nbsp;Tai-Ping Fan,&nbsp;Francesca Giampieri,&nbsp;Adil El Midaoui,&nbsp;Ka-Hing Wong,&nbsp;Ren-You Gan,&nbsp;Ahmed Fatimi,&nbsp;Atanas G. Atanasov","doi":"10.1002/imt2.70028","DOIUrl":"https://doi.org/10.1002/imt2.70028","url":null,"abstract":"<p>The microbiota, comprising all the microorganisms within the body, plays a critical role in maintaining good health. Dysbiosis represents a condition resulting from an imbalance or alteration of the microbiota. This study comprehensively investigates the patent literature on dysbiosis over the past 20 years.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"4 3","pages":""},"PeriodicalIF":23.7,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.70028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative cross-tissue analysis unveils complement-immunoglobulin augmentation and dysbiosis-related fatty acid metabolic remodeling during mammalian aging","authors":"Feng Zhang,&nbsp;Rong Li,&nbsp;Yasong Liu,&nbsp;Jinliang Liang,&nbsp;Yihang Gong,&nbsp;Cuicui Xiao,&nbsp;Jianye Cai,&nbsp;Tingting Wang,&nbsp;Qiang You,&nbsp;Jiebin Zhang,&nbsp;Haitian Chen,&nbsp;Jiaqi Xiao,&nbsp;Yingcai Zhang,&nbsp;Yang Yang,&nbsp;Hua Li,&nbsp;Jia Yao,&nbsp;Qi Zhang,&nbsp;Jun Zheng","doi":"10.1002/imt2.70027","DOIUrl":"https://doi.org/10.1002/imt2.70027","url":null,"abstract":"<p>Aging-related decline and adaptation are complex, multifaceted processes that affect various tissues and increase risk of chronic diseases. To characterize key changes in cross-tissue aging, we performed comprehensive proteomic and metabolomic analyses across 21 solid tissues and plasma samples, alongside shotgun metagenomic profiling of fecal microbial communities in young and aged mice. Our findings revealed widespread aging-rewired chronic inflammation, characterized by complement system activation in plasma and universal immunoglobulins accumulation across multiple solid tissues. This inflammatory remodeling significantly enhanced vulnerability to aging-related tissue injury. Moreover, we identified organ-specific and organ-enriched proteins with high functional specificity. Among these, aging-related proteins were closely linked to disorders arising from lipid metabolism dysfunction. Analysis of multi-tissue metabolomic and fecal metagenomic profiles revealed that aging significantly disrupted inter-tissue metabolic coupling, activities of polyunsaturated fatty acids metabolism, and gut microbiota homeostasis. Aged mice exhibited a marked decrease in <i>Escherichia</i> and an increase in <i>Helicobacter</i>, strongly correlating with alterations in omega-3 and omega-6 fatty acid abundances. Through multi-omics integration, we identified key molecular hubs driving organismal responses to aging. Collectively, our study uncovers extensive aging-associated alterations across tissues, emphasizing the interplay between systemic inflammation and dysbiosis-driven fatty acid remodeling. These findings provide deeper insights into the development of healthy aging from a cross-tissue perspective.</p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"4 3","pages":""},"PeriodicalIF":23.7,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.70027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peripheral blood immune landscape and NXPE3 as a novel biomarker for hypertensive intracerebral hemorrhage risk prediction and targeted therapy","authors":"Meng Zhang,&nbsp;Jingyuan Ning,&nbsp;Jing Liu,&nbsp;Yingying Sun,&nbsp;Ning Xiao,&nbsp;Haochen Xu,&nbsp;Jingzhou Chen","doi":"10.1002/imt2.70030","DOIUrl":"https://doi.org/10.1002/imt2.70030","url":null,"abstract":"<p>We employed bulk RNA-seq and scRNA-seq techniques to analyze the immune dysregulation in patients with intracerebral hemorrhage (ICH). The study revealed that excessive inflammatory responses, neutrophil activation, and T-cell dysfunction are the main characteristics of ICH. A multi-machine learning framework was utilized to construct a predictive model for ICH risk in hypertensive patients. Molecular docking and simulation results demonstrated that NXPE3 is a potential therapeutic target, and dihydroergotamine (DHE) exhibits a strong binding affinity to NXPE3. In vivo experiments indicated that DHE can reduce the incidence of spontaneous ICH and modulate the immune-inflammatory response. These results support DHE targeting NXPE3 as a potential therapeutic strategy for hypertension-related ICH.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"4 3","pages":""},"PeriodicalIF":23.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.70030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A panoramic view of cotton resistance to Verticillium dahliae: From genetic architectures to precision genomic selection","authors":"Xiaojun Zhang,&nbsp;Shiming Liu,&nbsp;Peng Wu,&nbsp;Wanying Xu,&nbsp;Dingyi Yang,&nbsp;Yuqing Ming,&nbsp;Shenghua Xiao,&nbsp;Weiran Wang,&nbsp;Jun Ma,&nbsp;Xinhui Nie,&nbsp;Zhan Gao,&nbsp;Junyuan Lv,&nbsp;Fei Wu,&nbsp;Zhaoguang Yang,&nbsp;Baoxin Zheng,&nbsp;Ping Du,&nbsp;Jiangmei Wang,&nbsp;Hao Ding,&nbsp;Jie Kong,&nbsp;Alifu Aierxi,&nbsp;Yu Yu,&nbsp;Wei Gao,&nbsp;Zhongxu Lin,&nbsp;Chunyuan You,&nbsp;Keith Lindsey,&nbsp;Nataša Štajner,&nbsp;Maojun Wang,&nbsp;Jiahe Wu,&nbsp;Shuangxia Jin,&nbsp;Xianlong Zhang,&nbsp;Longfu Zhu","doi":"10.1002/imt2.70029","DOIUrl":"https://doi.org/10.1002/imt2.70029","url":null,"abstract":"<p>Investigating the genetic regulatory mechanisms underlying complex traits forms the foundation for crop improvement. Verticillium wilt (VW), caused by <i>Verticillium dahlia</i>e (<i>V. dahliae</i>), is one of the most devastating diseases affecting crop production worldwide. However, the genetic basis underlying crop resistance to <i>V. dahliae</i> remains largely obscure, hindering progress in the genomic selection for VW resistance breeding. Here, we unraveled the genetic architectures and regulatory landscape of VW resistance in cotton by combining genome-wide association studies (GWAS) and transcriptome-wide association studies (TWAS) using 1152 transcriptomes derived from 290 cotton accessions. We identified 10 reliable quantitative trait loci (QTLs) associated with VW resistance across multiple environments. These QTLs showed a pyramiding resistance effect and exhibited promising efficacy in the genomic prediction of cotton's VW resistance supported by an F_2:3 population. Moreover, trace analysis of these elite alleles revealed a notably increased utilization of Lsnp1, Lsnp4, Lsnp5, Lsnp8, and Lsnp9, which potentially contribute to the improvement of VW resistance in Chinese cotton breeding since the 1990s. We also identified remarkable gene modules and expression QTL (eQTL) hotspots related to the regulation of reactive oxygen species (ROS) homeostasis and immune response. Furthermore, 15 candidate causal genes were prioritized by TWAS. Knocking down eight genes with a negative effect significantly enhanced cotton resistance to <i>V. dahliae</i>. Among them, <i>GhARM</i>, encoding an armadillo (ARM)-repeat protein, was verified to modulate cotton resistance to <i>V. dahliae</i> by regulating ROS homeostasis. Overall, this study updates the understanding of the genetic basis and regulatory mechanisms of cotton's VW resistance, providing valuable strategies for VW management through genomic selection in cotton breeding.</p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"4 3","pages":""},"PeriodicalIF":23.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.70029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histamine: A key compound in red light-enhanced Fusarium verticillioides resistance in maize","authors":"Xuanjun Feng,&nbsp;Dan Zheng,&nbsp;Weixiao Zhang,&nbsp;Huihui Xiao,&nbsp;Huarui Guan,&nbsp;Hao Xiong,&nbsp;Li Jia,&nbsp;Xuemei Zhang,&nbsp;Wenming Wang,&nbsp;Haiyang Wang,&nbsp;Yanli Lu","doi":"10.1002/imt2.70020","DOIUrl":"https://doi.org/10.1002/imt2.70020","url":null,"abstract":"<p>In this study, we demonstrate that red light is the most critical light component for promoting healthy maize growth during <i>Fusarium verticillioides</i> infection. Red light receptors <i>PHYTOCHROME B</i> (<i>PHYB</i>) and <i>C</i> (<i>PHYC</i>) play essential roles in maize defense against this pathogen. Overexpression of <i>PHYC</i> in maize enhances resistance to <i>F. verticillioides</i>. Additionally, we identified two defense-related gene networks and some metabolites that reliant on <i>PHYC</i>s, involving key contributors such as <i>WRKY</i> transcription factors and metabolites like histamine and thiamine. Notably, the application of 50 μM histamine significantly boosts resistance, particularly under high-density conditions, marking the first report of the role of histamine in disease resistance in plants.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"4 2","pages":""},"PeriodicalIF":23.7,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.70020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"QSCNAS: A platform for quorum sensing and quenching bacteria analysis in global wastewater treatment plants","authors":"Yong-Chao Wang,&nbsp;Sen Wang,&nbsp;Ya-Hui Lv,&nbsp;Can Wang","doi":"10.1002/imt2.70026","DOIUrl":"https://doi.org/10.1002/imt2.70026","url":null,"abstract":"<p>This study identifies the potential quorum sensing (QS) bacteria in wastewater treatment plants (WWTPs) and constructs a QS communication network through the establishment of a local QS bacterial database with six languages and the analysis of over 1000 activated sludge microbiome samples collected from 269 WWTPs. The results not only advance the understanding of bacterial communication in WWTPs but also provide a valuable tool for developing regulatory strategies to optimize the functionality of these vital ecosystems.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"4 3","pages":""},"PeriodicalIF":23.7,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.70026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}