iMeta最新文献

{"title":"Monocyte inflammation and adaptive immune dysfunction in severe <i>Klebsiella pneumoniae</i> pneumonia.","authors":"Hongquan Chen, Xiaoling Wang, Xiang Liu, Kui Dong, Yanying Feng, Min Yuan, Shiyi Wang, Fangfang Fan, Yong Tian, Zhiming Kang, Xiushuai Du, Chaolu Hasi, Hong Lu, Wei Guan, Xu Chen, Mengyu Cheng, Wei Wei, Nan Guo, Zhiguo Liu, Xiaotong Qiu, Yiwei Shi, Kun Xiao, Yi Wang, Zhenjun Li","doi":"10.1002/imt2.70172","DOIUrl":"10.1002/imt2.70172","url":null,"abstract":"<p><p>Severe <i>Klebsiella pneumoniae</i> pneumonia is associated with substantial mortality and is characterized by intense systemic inflammation that coexists with a failure to control infection. The cellular changes underlying the transition from effective host defense to immune dysfunction remain incompletely defined. Here, we constructed a severity-focused single-cell transcriptomic atlas of 708,894 peripheral blood mononuclear cells from 100 individuals (54 healthy controls, 23 patients with mild disease, and 23 patients with severe disease), complemented by plasma protein measurements and flow cytometry. Mild disease was characterized by a relatively coordinated peripheral immune response, with preserved monocyte regulatory and tissue-repair programs, preserved cytotoxic lymphocyte activity, and coordinated interactions between T follicular helper cells and B cells. Severe disease showed marked monocyte inflammation together with widespread disruption of adaptive immune responses. Classical monocytes expanded and showed increased expression of <i>S100A8/A9/A12</i>, accompanied by enhanced <i>TLR4-MYD88</i> signaling and elevated plasma S100 proteins. These changes were accompanied by impaired dendritic cell antigen presentation, expansion of immunoregulatory monocyte states resembling monocytic myeloid-derived suppressor cells, cytotoxicity- and exhaustion-associated changes in CD8⁺ T cells and natural killer cells, hyperactivated yet poorly coordinated CD4⁺ T cell states, and weaker B cell receptor signaling despite plasma cell expansion. These findings delineate a severity-associated peripheral immune state characterized by monocyte-centered inflammatory signatures and features of altered adaptive immune coordination, and provide a basis for future mechanistic investigation of monocyte-derived <i>S100</i> alarmins and severity-associated myeloid remodeling.</p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":" ","pages":"e70172"},"PeriodicalIF":44.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13594409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149026889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding sequence recognition code of nucleic acid-binding proteins of human-infecting DNA viruses.","authors":"Meifang Tang, Shifei Yang, Haiqian Ma, Ruoying Guo, Jie Chen, Hui Zhang, Jinghan Zhang, Wei Sun, Yun Wei, Ligang Fan, Jian Yan","doi":"10.1002/imt2.70170","DOIUrl":"10.1002/imt2.70170","url":null,"abstract":"<p><p>Human-infecting DNA viruses remain major health threats, yet the DNA-recognition mechanisms of their nucleic acid-binding proteins (NBPs) are poorly understood. Here, we systematically profiled 103 viral NBPs from human-infecting DNA viruses, with three NBPs from non-human-infecting DNA viruses as controls, using high-throughput screening. This analysis identified diverse DNA-binding motifs and specificity modules, including convergent recognition of a conserved CCACC motif across phylogenetically distant viruses. Notably, viral NBP binding-site distributions varied with genome size, and several NBPs from small-genome viruses showed enrichment on mitochondrial DNA. Functional assays further supported their mitochondrial association and effects on mitochondrial membrane potential. By integrating an ivTRT-based ssDNA-SELEX workflow, we further found that ssDNA viral NBPs recognize dimer-like and inverted-repeat sequences with potential to form stem-loop structures. Collectively, this study constructs a comprehensive viral NBP DNA-recognition atlas, offering a fundamental resource for elucidating viral genome recognition mechanisms, virus-mitochondria interactions, and developing future antiviral strategies.</p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":" ","pages":"e70170"},"PeriodicalIF":44.4,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13550974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148914574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil-derived, gut-dominant generalist bacteria shape the fitness of folivorous larvae.","authors":"Yu-Xuan Zheng, Ying Wang, Xiao-Man Zhang, Qian Jin, Yan Zhang, Gui-Fang Wang, Yong-Qi Shao, Jing Li, Cheng Sun, Wei-Dong Kong, Cai-Qing Yang, Ai-Bing Zhang","doi":"10.1002/imt2.70169","DOIUrl":"10.1002/imt2.70169","url":null,"abstract":"<p><p>Whether Lepidoptera harbor a conserved core gut microbiome has long remained contentious. Through large-scale microbiome profiling of folivorous larvae, their host plants, and associated soils across three climatically distinct regions of China, we identify two soil-derived generalist bacteria, <i>Ralstonia insidiosa</i> and <i>Delftia</i> sp., that colonize 97.92% of larval species examined, attaining mean relative abundances exceeding 47%, with the soil microbial reservoir as their principal source. Strikingly, these two taxa exhibit strong mutual exclusion within the larval gut yet govern host development through diametrically opposed metabolic strategies: <i>R. insidiosa</i> promotes larval weight gain, whereas <i>Delftia</i> sp. suppresses growth. This functional bifurcation, in which two widespread generalists exert opposite phenotypic effects, represents a previously undescribed phenomenon in insect-microbe symbiosis. Our findings provide broad evidence that soil microbial reservoirs can shape aboveground herbivore fitness via horizontally acquired bacteria, offering mechanistic insights for microbiome-based ecological management.</p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":" ","pages":"e70169"},"PeriodicalIF":44.4,"publicationDate":"2026-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13527294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wekemo Bioincloud 2026: An AI-enabled platform for standardized multi-omics data analyses","authors":"Yunyun Gao,&nbsp;Guoxing Zhang,&nbsp;Chengrui Wang,&nbsp;Hao Xu,&nbsp;Zhanhui Yu,&nbsp;Shunyao Jiang,&nbsp;Yong-Xin Liu","doi":"10.1002/imt2.70149","DOIUrl":"https://doi.org/10.1002/imt2.70149","url":null,"abstract":"<p>The rapid expansion of multi-omics data has created an increasing demand for accessible and reproducible platforms for integrative analysis. Here, we present Wekemo Bioincloud 2026, a comprehensive upgrade of the original platform, providing 41 standardized workflows (expanded from 22) across nine analytical categories, including a newly introduced correlation analysis module for cross-omics association studies. Visualization capabilities have been substantially expanded to 126 tools (from 65), incorporating artificial intelligence (AI)-guided parameter selection and automated image interpretation to enhance data exploration and result interpretability. In addition, Wekemo Bioincloud 2026 integrates an AI-powered research platform that supports the full research lifecycle, including automated literature synthesis, hypothesis generation, intelligent question answering, experimental design, and journal recommendation. Together, these advances reduce technical barriers, improve reproducibility and interpretability, and enable end-to-end AI-assisted multi-omics research. The platform is freely accessible at https://www.bioincloud.tech/ and supports a broad range of biological and biomedical applications.</p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"5 4","pages":""},"PeriodicalIF":44.4,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.70149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148816568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary fiber-based oral nanovaccine promotes gut-to-nose trafficking of regulatory cells and drives antigen-specific tolerance to relieve airway allergy","authors":"Yuting Qin,&nbsp;Zeming Wang,&nbsp;Yuru Zong,&nbsp;Zefang Lu,&nbsp;Junxiu Liu,&nbsp;Ruifang Zhao,&nbsp;Guangjun Nie,&nbsp;Hanqing Chen","doi":"10.1002/imt2.70158","DOIUrl":"10.1002/imt2.70158","url":null,"abstract":"<p>Current therapies for allergic rhinitis primarily provide symptomatic relief but fail to establish durable antigen-specific immune tolerance. Here, we developed an oral nano-dietary fiber (NDF) platform that integrates allergen delivery with microbiota-driven immunomodulation, using dextran as both an antigen carrier and a fermentable substrate for intestinal bacteria. NDF enabled coordinated allergen release and sustained short-chain fatty acid (SCFA) production in the gut, promoting SCFA–FFAR2-dependent expansion of regulatory B and T cells and their trafficking to the nasal mucosa. In murine models, NDF slowed disease progression, improved airway function, and reduced allergen-specific IgE levels. Together, these findings establish NDF as an oral tolerogenic vaccine that provides durable protection against allergic airway disease through the gut-nasal axis.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"5 4","pages":""},"PeriodicalIF":44.4,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13401610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148594729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bazi Bushen Capsule restores fertility by targeting mitochondrial health in aging endometrium","authors":"Shangqi Li,&nbsp;Feng Deng,&nbsp;Hongjuan Niu,&nbsp;Meng Li,&nbsp;Zeyang Lin,&nbsp;Jiayi Ma,&nbsp;Yue Wang,&nbsp;Yueqi Leng,&nbsp;Yunlong Hou,&nbsp;Wenwen Cui,&nbsp;Yang Yu,&nbsp;Heng Pan,&nbsp;Ping Zhou,&nbsp;Rong Li","doi":"10.1002/imt2.70154","DOIUrl":"10.1002/imt2.70154","url":null,"abstract":"<p>Age-related decline in endometrial function is a major cause of fertility issues in women, but effective treatments are lacking. This study aimed to investigate the impact of Bazi Bushen Capsule (BZBS) on endometrial aging and its underlying mechanisms. Using naturally aged mice and <span>d</span>-galactose-induced senescent human endometrial stromal cell lines, along with primary human endometrial stromal cells, we explored the effects of BZBS through single-cell RNA sequencing, transcriptome analysis, network pharmacology, and molecular dynamics simulations. Our results showed that BZBS improved endometrial receptivity, and decidualization by activating the AMPK−SIRT3 pathway. The key bioactive components of BZBS were identified as ginsenosides Rb1, Rb2, Rg5, and luteolin. Specifically, luteolin restored mitochondrial homeostasis by regulating PINK1/Parkin−mediated mitophagy and inhibiting Drp1. These findings suggested that BZBS effectively restored endometrial function and offered a promising therapeutic approach to counteract age-related endometrial dysfunction, potentially improving fertility in women of advanced maternal age.</p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"5 4","pages":""},"PeriodicalIF":44.4,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13416912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal and infant gut microbiome","authors":"Huidi Wang,&nbsp;Felicia Tin,&nbsp;Hui Chen,&nbsp;Fuxin Jiao,&nbsp;Min Wu,&nbsp;Shanshan Sun,&nbsp;Lisha Lin,&nbsp;Die Li,&nbsp;Huimin Zheng,&nbsp;Zhenxin Niu,&nbsp;Mengyao Lan,&nbsp;Bahtiyar Yilmaz,&nbsp;Johan G. Eriksson,&nbsp;Mengjia Wang,&nbsp;Andrew Macpherson,&nbsp;Jose C. Clemente,&nbsp;Jia Xu,&nbsp;Ri-hua Xie,&nbsp;Xiaojiao Zheng,&nbsp;Tao Zhou,&nbsp;Jinfeng Wang,&nbsp;Wei Shen,&nbsp;Bing Huang,&nbsp;Xia Chen,&nbsp;Hai Li,&nbsp;Yan He","doi":"10.1002/imt2.70151","DOIUrl":"10.1002/imt2.70151","url":null,"abstract":"<p>Early-life gut microbiome assembly is a pivotal determinant of lifelong health; however, the integrated frameworks governing this process across developmental milestones remain insufficiently defined. This review establishes a multidimensional framework by delineating the crosstalk between the gut microbiome and the host throughout the preconception, prenatal, postpartum, and early childhood stages. We first highlight the emerging paradigm of biparental microbial contributions during the preconception period, detailing how paternal and maternal niches jointly prime offspring development. Moving into pregnancy, we examine the maternal reservoir, integrating the role of gut microbiota-derived metabolites across multiple trimesters in prenatal priming and vertical transmission. For the postpartum period, we discuss the development of the multikingdom gut microbiome and address the impacts of delivery modes and clinical interventions. Here, we articulate a critical knowledge gap: the discrepancy between taxonomic “catch-up” and true functional restoration, particularly in vulnerable cohorts such as preterm infants. Furthermore, we propose a “developmental synchronization” model within the maternal–infant–microbiome continuum. This model posits that early-life “windows of opportunity” are defined by the obligate temporal coupling of host physiological maturation with stage-specific microbial metabolic signals. From a translational perspective, we discuss how this framework informs the development of precision interventions, such as stage-specific probiotics, prebiotics, or metabolic modulators. These therapies aim to restore not only the microbial composition but also the synchronized functional dialog between the microbiome and host development. By mapping the “microbiota–metabolite–host target–physiological phenotype” network, we provide a systematic roadmap for precision-targeted interventions during the first 1000 days of life.</p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"5 4","pages":""},"PeriodicalIF":44.4,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13437732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148681240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The gut microbiome organ","authors":"Yang Bi,&nbsp;Weibin Song,&nbsp;Maria Glymenaki,&nbsp;Jie Hu,&nbsp;Xiru Li,&nbsp;Heng Huang,&nbsp;Yousong Peng,&nbsp;Shuaishuai Ni,&nbsp;Ornuma Haonon,&nbsp;Zhu Liu,&nbsp;Krai Daowtak,&nbsp;Xiaotao Shen,&nbsp;Huadong Peng,&nbsp;Jutarop Phetcharaburanin,&nbsp;Huiru Tang,&nbsp;Yulan Wang,&nbsp;Jia V. Li,&nbsp;Mizu Jiang,&nbsp;Nick Powell,&nbsp;Victor W. Zhong,&nbsp;Elaine Holmes,&nbsp;Yunlei Xianyu,&nbsp;Zhigang Liu","doi":"10.1002/imt2.70144","DOIUrl":"10.1002/imt2.70144","url":null,"abstract":"<p>The human gut microbiome is increasingly viewed as an active regulator of host physiology, extending beyond earlier taxonomy-centered descriptions of a complex microbial community. Accumulating evidence supports an organ-like conceptual framework in which the gut microbiome exhibits spatially structured organization, extensive metabolic capacity, and continuous bidirectional communication with host systems. Through the production of bioactive metabolites with endocrine-like, immunomodulatory, and neuromodulatory properties, the microbiome contributes to metabolic, immune, and neuroendocrine regulation, thereby influencing systemic homeostasis and disease susceptibility. Recent advances in multi-omics, spatial biology, and computational modeling are moving the field from taxonomic association toward functional interpretation, mechanistic insight, and causal inference. These approaches are beginning to reveal microbiome-derived functional modules and host–microbe signaling networks that are shaped by host genetics, diet, medications, feeding patterns, circadian rhythms, and environmental exposures. In this review, we synthesize current mechanistic and translational evidence to conceptualize the gut microbiome as an organ-like functional system, delineate its structural and functional organization, and propose a framework for mapping, modeling, and therapeutically targeting microbiome-derived circuits to support precision medicine in metabolic, inflammatory, and selected gut–brain axis-related disorders.</p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"5 4","pages":""},"PeriodicalIF":44.4,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13496164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148802440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental chemicals and the tumor immune microenvironment","authors":"Tongtong Zhang,&nbsp;Yanxia Hu,&nbsp;Weiran Zhang,&nbsp;Yang Jin,&nbsp;Chaoyang Sun,&nbsp;Afshin Samali,&nbsp;Shunqing Xu,&nbsp;Xia Sheng","doi":"10.1002/imt2.70160","DOIUrl":"https://doi.org/10.1002/imt2.70160","url":null,"abstract":"<p>Impact of representative environmental chemicals on tumor immune microenvironment (TIME). Representative environmental chemicals are ubiquitously distributed across air, soil, aquatic systems, and biota. These toxicants enter the human body via multiple exposure routes, including inhalation, dietary intake, and dermal absorption, subsequently accumulating in vital organs (such as the liver, lungs, kidneys, and intestines) as well as within tumor tissues. By disrupting physiological homeostasis, they impair the functionality of critical biological systems, including the metabolic, nervous, circulatory, and immune systems. Notably, within tumor tissues, these chemicals remodel the TIME by suppressing antitumor immunity and compromising the efficacy of immunotherapy, thereby promoting tumor initiation and progression.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"5 4","pages":""},"PeriodicalIF":44.4,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.70160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148816547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial DNA methylation links rumen transcriptional activity to methane emission divergence in dairy cows","authors":"Xinyue Zhang,&nbsp;Guirong Xu,&nbsp;Liwei Zhao,&nbsp;Ye Tao,&nbsp;Jiawei Zhang,&nbsp;Cameron E. F. Clark,&nbsp;Yangyi Hao,&nbsp;Zhijun Cao,&nbsp;Shengli Li,&nbsp;Wei Wang","doi":"10.1002/imt2.70153","DOIUrl":"https://doi.org/10.1002/imt2.70153","url":null,"abstract":"<p>Genome-resolved methylomics uncovers the first DNA methylation atlas of the dairy cow rumen microbiome. Microbial DNA methylation profiles diverge between low- and high- methane-emitting dairy cows despite limited taxonomic differences. Differential methylation is linked to transcriptional variation in genes involved in carbon metabolism, hydrogen utilization, and methanogenesis.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"5 4","pages":""},"PeriodicalIF":44.4,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.70153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148816573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}