iMeta Pub Date : 2024-03-19 DOI: 10.1002/imt2.185

Tao Wang, Penghao Li, Xue Bai, Shilin Tian, Maosen Yang, Dong Leng, Hua Kui, Sujuan Zhang, Xiaomiao Yan, Qu Zheng, Pulin Luo, Changming He, Yan Jia, Zhoulin Wu, Huimin Qiu, Jing Li, Feng Wan, Muhammad A. Ali, Rurong Mao, Yong-Xin Liu, Diyan Li

{"title":"Vaginal microbiota are associated with in vitro fertilization during female infertility","authors":"Tao Wang, Penghao Li, Xue Bai, Shilin Tian, Maosen Yang, Dong Leng, Hua Kui, Sujuan Zhang, Xiaomiao Yan, Qu Zheng, Pulin Luo, Changming He, Yan Jia, Zhoulin Wu, Huimin Qiu, Jing Li, Feng Wan, Muhammad A. Ali, Rurong Mao, Yong-Xin Liu, Diyan Li","doi":"10.1002/imt2.185","DOIUrl":"10.1002/imt2.185","url":null,"abstract":"The vaginal microbiome plays an essential role in the reproductive health of human females. As infertility increases worldwide, understanding the roles that the vaginal microbiome may have in infertility and in vitro fertilization (IVF) treatment outcomes is critical. To determine the vaginal microbiome composition of 1411 individuals (1255 undergoing embryo transplantation) and their associations with reproductive outcomes, clinical and biochemical features are measured, and vaginal samples are 16S rRNA sequenced. Our results suggest that both too high and too low abundance of Lactobacillus is not beneficial for pregnancy; a moderate abundance is more beneficial. A moderate abundance of Lactobacillus crispatus and Lactobacillus iners (~80%) (with a pregnancy rate of I-B: 54.35% and III-B: 57.73%) is found beneficial for pregnancy outcomes compared with a higher abundance (>90%) of Lactobacillus (I-A: 44.81% and III-A: 51.06%, respectively). The community state type (CST) IV-B (contains a high to moderate relative abundance of Gardnerella vaginalis) shows a similar pregnant ratio (48.09%) with I-A and III-A, and the pregnant women in this CST have a higher abundance of Lactobacillus species. Metagenome analysis of 71 samples shows that nonpregnant women are detected with more antibiotic-resistance genes, and Proteobacteria and Firmicutes are the main hosts. The inherent differences within and between women in different infertility groups suggest that vaginal microbes might be used to detect infertility and potentially improve IVF outcomes.","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"3 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.185","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140228996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering unique and shared interactions between the human gut microbiota and oral antidiabetic drugs 解密人类肠道微生物群与口服抗糖尿病药物之间独特和共同的相互作用

iMeta Pub Date : 2024-03-13 DOI: 10.1002/imt2.179

Huahui Ren, Zhun Shi, Fangming Yang, Shujie Wang, Fengyi Yuan, Tingting Li, Min Li, Jiahui Zhu, Junhua Li, Kui Wu, Yifei Zhang, Guang Ning, Karsten Kristiansen, Weiqing Wang, Yanyun Gu, Huanzi Zhong

引用次数: 0

The gut microbiota-aromatic hydrocarbon receptor (AhR) axis mediates the anticolitic effect of polyphenol-rich extracts from Sanghuangporus 肠道微生物群-芳香烃受体（AhR）轴介导桑黄菌富含多酚的提取物的抗胆碱作用

iMeta Pub Date : 2024-03-11 DOI: 10.1002/imt2.180

Shi Zhong, Yu-Qing Sun, Jin-Xi Huo, Wen-Yi Xu, Ya-Nan Yang, Jun-Bo Yang, Wei-Jie Wu, Yong-Xin Liu, Chong-Ming Wu, You-Gui Li

{"title":"The gut microbiota-aromatic hydrocarbon receptor (AhR) axis mediates the anticolitic effect of polyphenol-rich extracts from Sanghuangporus","authors":"Shi Zhong, Yu-Qing Sun, Jin-Xi Huo, Wen-Yi Xu, Ya-Nan Yang, Jun-Bo Yang, Wei-Jie Wu, Yong-Xin Liu, Chong-Ming Wu, You-Gui Li","doi":"10.1002/imt2.180","DOIUrl":"10.1002/imt2.180","url":null,"abstract":"Inflammatory bowel disease (IBD) is a significant global health concern. The gut microbiota plays an essential role in the onset and development of IBD. Sanghuangporus (SH), a traditional Chinese medicinal mushroom, has excellent anti-inflammatory effects and is effective at modulating the gut microbiota. Despite these attributes, the specific anticolitic effects of SH and the mechanisms through which the gut microbiota mediates its benefits remain unclear. Herein, we demonstrated that polyphenol-rich extract from SH effectively alleviated the pathological symptoms of dextran sodium sulfate (DSS)-induced colitis in mice by modulating the gut microbiota. Treatment with SH distinctly enriched Alistipes, especially Alistipes onderdonkii, and its metabolite 5-hydroxyindole-3-acetic acid (5HIAA). Oral gavage of live A. onderdonkii or 5HIAA potently mitigated DSS-induced colitis in mice. Moreover, both 5HIAA and SH significantly activated the aromatic hydrocarbon receptor (AhR), and the administration of an AhR antagonist abrogated their protective effects against colitis. These results underscore the potent efficacy of SH in diminishing DSS-induced colitis through the promotion of A. onderdonkii and 5HIAA, ultimately activating AhR signaling. This study unveils potential avenues for developing therapeutic strategies for colitis based on the interplay between SH and the gut microbiota.","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.180","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140254266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ChatGPT and generative AI are revolutionizing the scientific community: A Janus-faced conundrum ChatGPT 和生成式人工智能正在科学界掀起一场革命：雅努斯式难题

iMeta Pub Date : 2024-02-27 DOI: 10.1002/imt2.178

Zhongji Pu, Chun-Lin Shi, Che Ok Jeon, Jingyuan Fu, Shuang-Jiang Liu, Canhui Lan, Yanlai Yao, Yong-Xin Liu, Baolei Jia

引用次数: 0

Photoaffinity probe-based antimalarial target identification of artemisinin in the intraerythrocytic developmental cycle of Plasmodium falciparum 基于光亲和探针的青蒿素在恶性疟原虫红细胞内发育周期中的抗疟靶标鉴定

iMeta Pub Date : 2024-02-19 DOI: 10.1002/imt2.176

Peng Gao, Jianyou Wang, Chong Qiu, Huimin Zhang, Chen Wang, Ying Zhang, Peng Sun, Honglin Chen, Yin Kwan Wong, Jiayun Chen, Junzhe Zhang, Huan Tang, Qiaoli Shi, Yongping Zhu, Shengnan Shen, Guang Han, Chengchao Xu, Lingyun Dai, Jigang Wang

{"title":"Photoaffinity probe-based antimalarial target identification of artemisinin in the intraerythrocytic developmental cycle of Plasmodium falciparum","authors":"Peng Gao, Jianyou Wang, Chong Qiu, Huimin Zhang, Chen Wang, Ying Zhang, Peng Sun, Honglin Chen, Yin Kwan Wong, Jiayun Chen, Junzhe Zhang, Huan Tang, Qiaoli Shi, Yongping Zhu, Shengnan Shen, Guang Han, Chengchao Xu, Lingyun Dai, Jigang Wang","doi":"10.1002/imt2.176","DOIUrl":"https://doi.org/10.1002/imt2.176","url":null,"abstract":"Malaria continues to pose a serious global health threat, and artemisinin remains the core drug for global malaria control. However, the situation of malaria resistance has become increasingly severe due to the emergence and spread of artemisinin resistance. In recent years, significant progress has been made in understanding the mechanism of action (MoA) of artemisinin. Prior research on the MoA of artemisinin mainly focused on covalently bound targets that are alkylated by artemisinin-free radicals. However, less attention has been given to the reversible noncovalent binding targets, and there is a paucity of information regarding artemisinin targets at different life cycle stages of the parasite. In this study, we identified the protein targets of artemisinin at different stages of the parasite's intraerythrocytic developmental cycle using a photoaffinity probe. Our findings demonstrate that artemisinin interacts with parasite proteins in vivo through both covalent and noncovalent modes. Extensive mechanistic studies were then conducted by integrating target validation, phenotypic studies, and untargeted metabolomics. The results suggest that protein synthesis, glycolysis, and oxidative homeostasis are critically involved in the antimalarial activities of artemisinin. In summary, this study provides fresh insights into the mechanisms underlying artemisinin's antimalarial effects and its protein targets.","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.176","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140559776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ggVennDiagram: Intuitive Venn diagram software extended ggVennDiagram：扩展的直观维恩图软件

iMeta Pub Date : 2024-02-14 DOI: 10.1002/imt2.177

Chun-Hui Gao, Chengjie Chen, Turgut Akyol, Adrian Dusa, Guangchuang Yu, Bin Cao, Peng Cai

引用次数: 0

Isolation of potentially novel species expands the genomic and functional diversity of Lachnospiraceae 潜在新物种的分离扩展了拉赫诺斯皮拉科（Lachnospiraceae）的基因组和功能多样性

iMeta Pub Date : 2024-02-13 DOI: 10.1002/imt2.174

Xiaoqian Lin, Tongyuan Hu, Zhinan Wu, Lingne Li, Yuhao Wang, Dingyang Wen, Xudong Liu, Wenxi Li, Hewei Liang, Xin Jin, Xun Xu, Jian Wang, Huanming Yang, Karsten Kristiansen, Liang Xiao, Yuanqiang Zou

{"title":"Isolation of potentially novel species expands the genomic and functional diversity of Lachnospiraceae","authors":"Xiaoqian Lin, Tongyuan Hu, Zhinan Wu, Lingne Li, Yuhao Wang, Dingyang Wen, Xudong Liu, Wenxi Li, Hewei Liang, Xin Jin, Xun Xu, Jian Wang, Huanming Yang, Karsten Kristiansen, Liang Xiao, Yuanqiang Zou","doi":"10.1002/imt2.174","DOIUrl":"10.1002/imt2.174","url":null,"abstract":"The Lachnospiraceae family holds promise as a source of next-generation probiotics, yet a comprehensive delineation of its diversity is lacking, hampering the identification of suitable strains for future applications. To address this knowledge gap, we conducted an in-depth genomic and functional analysis of 1868 high-quality genomes, combining data from public databases with our new isolates. This data set represented 387 colonization-selective species-level clusters, of which eight genera represented multilineage clusters. Pan-genome analysis, single-nucleotide polymorphism (SNP) identification, and probiotic functional predictions revealed that species taxonomy, habitats, and geography together shape the functional diversity of Lachnospiraceae. Moreover, analyses of associations with atherosclerotic cardiovascular disease (ACVD) and inflammatory bowel disease (IBD) indicated that several strains of potentially novel Lachnospiraceae species possess the capacity to reduce the abundance of opportunistic pathogens, thereby imparting potential health benefits. Our findings shed light on the untapped potential of novel species enabling knowledge-based selection of strains for the development of next-generation probiotics holding promise for improving human health and disease management.","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.174","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139840546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial load in meconium 胎粪中的细菌量

iMeta Pub Date : 2024-02-13 DOI: 10.1002/imt2.173

Wen-Yu Jin, Jing Peng, Jinping Dai, Rongkang Tang, Jia-Xin Guo, Huan Zhao, Jielin Wang, Shu Zhang, Yi-Zhou Gao

引用次数: 0

Wekemo Bioincloud: A user-friendly platform for meta-omics data analyses Wekemo Bioincloud：用户友好型元组学数据分析平台

iMeta Pub Date : 2024-02-13 DOI: 10.1002/imt2.175

Yunyun Gao, Guoxing Zhang, Shunyao Jiang, Yong-Xin Liu

引用次数: 0

Synthetic microbial communities: Sandbox and blueprint for soil health enhancement 合成微生物群落：增强土壤健康的沙盘和蓝图

iMeta Pub Date : 2024-02-11 DOI: 10.1002/imt2.172

Mei Li, Jie Hu, Zhong Wei, Alexandre Jousset, Thomas Pommier, Xiangyang Yu, Yangchun Xu, Qirong Shen

引用次数: 0

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