Human reproduction open最新文献

{"title":"Infertility and pregnancy outcomes among adults with primary ciliary dyskinesia.","authors":"Leonie D Schreck, Eva S L Pedersen, Katie Dexter, Michele Manion, Nathalie Massin, Bernard Maitre, Myrofora Goutaki, Claudia E Kuehni","doi":"10.1093/hropen/hoae039","DOIUrl":"10.1093/hropen/hoae039","url":null,"abstract":"<p><strong>Study question: </strong>What is the prevalence of infertility and ectopic pregnancies among individuals with primary ciliary dyskinesia (PCD)?</p><p><strong>Summary answer: </strong>We found that 39 of 50 men (78%) and 72 of 118 women (61%) with PCD were infertile and that women with PCD had an increased risk of ectopic pregnancies (7.6 per 100 pregnancies, 95% CI 4.7-12.2).</p><p><strong>What is known already: </strong>PCD is a heterogeneous multiorgan disease caused by mutations in genes required for the function and structure of motile cilia. Previous studies identified a link between PCD and infertility, but original data on prevalence of infertility and risk of ectopic pregnancies, the use and efficacy of medically assisted reproduction (MAR), and the association of fertility with PCD genotype are extremely limited.</p><p><strong>Study design size duration: </strong>We performed a cross-sectional survey about fertility within the <i>Living with PCD</i> study (formerly COVID-PCD). <i>Living with PCD</i> is an international, online, participatory study that collects information directly from people with PCD. People with PCD of any age from anywhere in the world can participate in the study. At the time of the survey, 482 adults with PCD were registered within the <i>Living with PCD</i> study.</p><p><strong>Participants/materials setting methods: </strong>We sent a questionnaire on fertility on 12 July 2022, to all participants older than 18 years enrolled in the <i>Living with PCD</i> study. Responses were collected until 8 March 2023. The fertility questionnaire covered topics related to pregnancy attempts, use of MAR, and pregnancy outcomes. Data were collected via the Research Electronic Data Capture (REDCap) platform. We defined infertility as failure to achieve a clinical pregnancy after 12 months or use of MAR for at least one pregnancy.</p><p><strong>Main results and the role of chance: </strong>In total, 265 of 482 adult participants (55%) completed the fertility questionnaire. Among 168 adults who had tried to conceive, 39 of 50 men (78%) and 72 of 118 women (61%) were infertile. Of the infertile men, 28 had tried MAR, and 17 of them (61%) fathered a child with the help of MAR. Among infertile women, 59 had used MAR, and 41 of them (69%) became pregnant with the help of MAR. In our population, women with PCD showed a relatively high risk of ectopic pregnancies: 1 in 10 women who became pregnant had at least one ectopic pregnancy and 7.6% of pregnancies were ectopic (95% CI 4.7-12.2). We evaluated the association between fertility and affected PCD genes in 46 individuals (11 men, 35 women) with available genetic and fertility information, and found differences between genotypes, e.g. all five women with a mutation in <i>CCDC40</i> were infertile and all five with <i>DNAH11</i> were fertile.</p><p><strong>Limitations reasons for caution: </strong>The study has limitations, including potential selection bias as people experie","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":null,"pages":null},"PeriodicalIF":8.3,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11219480/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive factors and their association with physical and comprehensive frailty in middle-aged and older women: a large-scale population-based study.","authors":"Wenting Hao, Qi Wang, Ruihong Yu, Shiva Raj Mishra, Salim S Virani, Nipun Shrestha, Chunying Fu, Dongshan Zhu","doi":"10.1093/hropen/hoae038","DOIUrl":"10.1093/hropen/hoae038","url":null,"abstract":"<p><strong>Study question: </strong>Are women's reproductive factors associated with physical frailty and comprehensive frailty in middle-age and later life?</p><p><strong>Summary answer: </strong>Early menarche at <13 years, age at menopause <45 years, surgical menopause, experiencing miscarriage and a shorter reproductive period of <35 years were associated with increased odds of frailty, while having two or three children was related to decreased likelihood of frailty.</p><p><strong>What is known already: </strong>Evidence has shown that women are frailer than men in all age groups and across different populations, although women have longer lifespans. Female-specific reproductive factors may be related to risk of frailty in women.</p><p><strong>Study design size duration: </strong>A population-based cross-sectional study involved 189 898 women from the UK Biobank.</p><p><strong>Participants/materials setting methods: </strong>Frailty phenotype and frailty index were used to assess physical frailty and comprehensive frailty (assessed using 38 health indicators for physical and mental wellbeing), respectively. Multivariable logistic regression models were used to estimate odds ratios (ORs) and 95% CI between reproductive factors and likelihood of physical frailty and comprehensive frailty. Restricted cubic spline models were used to test the non-linear associations between them. In addition, we examined the combined effect of categorized age at menopause and menopause hormone therapy (MHT) on frailty.</p><p><strong>Main results and the role of chance: </strong>There was a J-shape relationship between age at menarche, reproductive period, and frailty; age at menarche <13 years and >16 years, and reproductive period <35 years or >40 years were all associated with increased odds of frailty. There was a negative linear relationship between menopausal age (either natural or surgical) and odds of frailty. Surgical menopause was associated with 30% higher odds of physical frailty (1.34, 1.27-1.43) and 30% higher odds of comprehensive frailty (1.30, 1.25-1.35). Having two or three children was linked to the lowest likelihood of physical frailty (0.48, 0.38-0.59) and comprehensive frailty (0.72, 0.64-0.81). Experiencing a miscarriage increased the odds of frailty. MHT use was linked to increased odds of physical frailty in women with normal age at natural menopause (after 45 years), while no elevated likelihood was observed in women with early natural menopause taking MHT.</p><p><strong>Limitations reasons for caution: </strong>The reproductive factors were self-reported and the data might be subject to recall bias. We lacked information on the types and initiation time of MHT, could not identify infertile women who later became pregnant, and the number of infertile women may be underestimated. Individuals participating in the UK Biobank are not representative of the general UK population, limiting the generalization of our findings.</p><p><strong>Wider","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":null,"pages":null},"PeriodicalIF":8.3,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11211215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141473256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anxiety in couples undergoing IVF: evidence from E-Freeze randomised controlled trial","authors":"Yangmei Li, Jenny McLeish, Pollyanna Hardy, Christina Cole, Claire Carson, Fiona Alderdice, Abha Maheshwari","doi":"10.1093/hropen/hoae037","DOIUrl":"https://doi.org/10.1093/hropen/hoae037","url":null,"abstract":"\u0000 \u0000 \u0000 What are the risk factors and impacts of anxiety in women and men in heterosexual couples undergoing IVF as part of a randomised trial, with a delay in embryo transfer in one arm?\u0000 \u0000 \u0000 \u0000 Duration of infertility, ethnicity and male partner’s anxiety levels were associated with women’s anxiety at the start of treatment, while initial anxiety score, partner’s anxiety score at embryo transfer, ethnicity and clinic location were associated with women’s anxiety levels at embryo transfer; although women undergoing IVF were more anxious than their partners for slightly different reasons, their self-reported state anxiety was not associated with achieving clinical pregnancy, or with switching from delayed frozen embryo transfer to fresh embryo transfer in an IVF trial.\u0000 \u0000 \u0000 \u0000 Use of IVF treatment continues to rise and patients undergoing IVF are anxious. Participating in a randomised controlled trial (RCT) with uncertainty of arm randomisation might increase their anxiety, while a delay in treatment may add further to anxiety.\u0000 \u0000 \u0000 \u0000 A mixed methods study was conducted using data from the multi-centre E-Freeze RCT cohort conducted across 13 clinics in the UK from 2016 to 2019. A regression analysis on anxiety scores of couples undergoing the IVF trial and a qualitative analysis of participant questionnaires were performed.\u0000 \u0000 \u0000 \u0000 Six hundred and four couples participating in the E-Freeze trial, who had at least one useable State-Trait Anxiety Inventory (STAI) State Anxiety subscale (STAI-S) standardised self-report questionnaire for at least one of the partners, were included in the study. STAI-S scores were measured at consent for trial (T1) and again at embryo transfer (T2). Linear and log binomial regression were used to explore the association between characteristics and STAI-S scores, and the associations between STAI-S scores and non-compliance and clinical pregnancy, respectively. Responses to the open text question were qualitatively analysed inductively using content analysis.\u0000 \u0000 \u0000 \u0000 Women’s STAI-S scores at T1 (consent) were associated with their ethnicity, duration of infertility and their male partner’s STAI-S score at T1. Women’s STAI-S scores at T2 (embryo transfer) were associated with their ethnicity, location of fertility clinic, their STAI-S score at consent, and their male partner’s STAI-S score at embryo transfer. The adjusted coefficient (95%CI) for women’s STAI-S scores at T2 was -4.75 (-7.29, -2.20, p < 0.001) for ethnic minority versus White, -2.87 (-4.85, -0.89, p = 0.005) for Scotland versus England, 0.47 (0.37, 0.56, p < 0.001) for each point increase in their own score at T1, and 0.30 (0.21, 0.40, p < 0.001) for each point increase in their male partner’s score at T2. On average, women had higher STAI-S scores than men at both time points, and a larger increase of scores between the two time points. However, women’s STAI-S scores were not associated with either non-compliance with trial allocation in the ‘freeze-all’ tria","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141349268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endometriosis: from iron and macrophages to exosomes. Is the sky clearing?","authors":"Jacques Donnez, Marie-Madeleine Dolmans","doi":"10.1093/hropen/hoae034","DOIUrl":"10.1093/hropen/hoae034","url":null,"abstract":"","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":null,"pages":null},"PeriodicalIF":8.3,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11189659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141437889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"'There will be blood'^† A proof of concept for the role of haemorrhagic corpora lutea in the pathogenesis of endometriosis.","authors":"Paolo Vercellini, Camilla Erminia Maria Merli, Paola Viganò","doi":"10.1093/hropen/hoae035","DOIUrl":"10.1093/hropen/hoae035","url":null,"abstract":"","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":null,"pages":null},"PeriodicalIF":8.3,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11189658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141437890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-invasive imaging techniques for diagnosis of pelvic deep endometriosis and endometriosis classification systems: an International Consensus Statement<sup />.","authors":"G Condous, B Gerges, I Thomassin-Naggara, C Becker, C Tomassetti, H Krentel, B J van Herendael, M Malzoni, M S Abrao, E Saridogan, J Keckstein, G Hudelist","doi":"10.1093/hropen/hoae029","DOIUrl":"10.1093/hropen/hoae029","url":null,"abstract":"<p><p>The International Society of Ultrasound in Obstetrics and Gynecology (ISUOG) and International Deep Endometriosis Analysis (IDEA) group, the European Endometriosis League (EEL), the European Society for Gynaecological Endoscopy (ESGE), ESHRE, the International Society for Gynecologic Endoscopy (ISGE), the American Association of Gynecologic Laparoscopists (AAGL) and the European Society of Urogenital Radiology (ESUR) elected an international, multidisciplinary panel of gynecological surgeons, sonographers, and radiologists, including a steering committee, which searched the literature for relevant articles in order to review the literature and provide evidence-based and clinically relevant statements on the use of imaging techniques for non-invasive diagnosis and classification of pelvic deep endometriosis. Preliminary statements were drafted based on review of the relevant literature. Following two rounds of revisions and voting orchestrated by chairs of the participating societies, consensus statements were finalized. A final version of the document was then resubmitted to the society chairs for approval. Twenty statements were drafted, of which 14 reached strong and three moderate agreement after the first voting round. The remaining three statements were discussed by all members of the steering committee and society chairs and rephrased, followed by an additional round of voting. At the conclusion of the process, 14 statements had strong and five statements moderate agreement, with one statement left in equipoise. This consensus work aims to guide clinicians involved in treating women with suspected endometriosis during patient assessment, counselling, and planning of surgical treatment strategies.</p>","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11134890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141176476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of deep pelvic endometriosis following acute haemoperitoneum: a prospective ultrasound study.","authors":"Prubpreet Chaggar, Tina Tellum, Lucrezia Viola De Braud, Sarah Annie Solangon, Thulasi Setty, Davor Jurkovic","doi":"10.1093/hropen/hoae036","DOIUrl":"10.1093/hropen/hoae036","url":null,"abstract":"<p><strong>Study question: </strong>Is acute haemoperitoneum that is managed conservatively a precursor of deep endometriosis?</p><p><strong>Summary answer: </strong>Our study provides evidence to suggest that acute haemoperitoneum may lead to the development of deep endometriosis in a significant proportion of cases.</p><p><strong>What is known already: </strong>A recent pilot study was the first to suggest that acute haemoperitoneum could be a precursor of deep endometriosis. However, the sample size was small, and the follow-up was not standardized owing to unknown rates of clot absorption and development of endometriosis.</p><p><strong>Study design size duration: </strong>This was a prospective observational cohort study conducted at a single centre over a 31-month period. A required sample size of 30 was calculated using results from a previous study, with a minimum of 15 women each in the groups with and without significant haemoperitoneum (study and control groups, respectively). A total of 59 women were recruited to the study and eight were lost to follow-up. The final sample comprised 51 women, 15 in the study group and 36 in the control group.</p><p><strong>Participants/materials setting methods: </strong>All non-pregnant, premenopausal women aged 18-50 years who consecutively presented to our dedicated gynaecological diagnostic unit with severe acute lower abdominal pain were eligible for this study. We only included women who were clinically stable and were suitable for conservative management. Those with prior history or evidence of endometriosis on their initial ultrasound scan, previous hysterectomy, or bilateral oophorectomy were excluded. Participants had standardized follow-up visits for 6 months, with pelvic ultrasound scans and the British Society of Gynaecological Endoscopy pelvic pain questionnaires completed at each visit. The primary outcome was the sonographically confirmed presence of newly formed endometriosis. Secondary outcomes were the presence and change of pelvic pain symptoms and health-related quality of life (HR-QOL).</p><p><strong>Main results and the role of chance: </strong>After completion of follow-up, 7/15 (47%; 95% CI 21.3-71.4%) women presenting with acute haemoperitoneum (study group) developed sonographic evidence of deep endometriosis, compared to 0/36 (0%; 97.5% CI 0.0-9.7%) women in the control group. A ruptured functional haemorrhagic cyst was the most common cause of haemoperitoneum, occurring in 13/15 cases (87%). The time from the initial event to sonographic evidence of endometriosis varied from 2 to 6 months. The EuroQol visual analogue scores were not significantly different at baseline between the groups that developed and did not develop endometriosis [28 (interquartile range (IQR) 15-40, n = 6) vs 56 (IQR 35-75, n = 44), <i>P </i>=<i> </i>0.09], while the EuroQol-5D values were lower in the endometriosis group [-0.01 (IQR -0.07 to 0.19, n = 6) vs 0.62 (IQR 0.24-0.73, n = 44), <i>P </i>=<i>","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":null,"pages":null},"PeriodicalIF":8.3,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11189661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141437888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiovascular disease risk factors and infertility: multivariable analyses and one-sample mendelian randomisation analyses in the trøndelag health study","authors":"Karoline H Skåra, Álvaro Hernáez, Øyvind Næss, Abigail Fraser, Deborah A Lawlor, S. Burgess, Ben Brumpton, Maria C Magnus","doi":"10.1093/hropen/hoae033","DOIUrl":"https://doi.org/10.1093/hropen/hoae033","url":null,"abstract":"\u0000 \u0000 \u0000 Are cardiovascular disease (CVD) risk factors causally associated with higher risk of infertility among women and men?\u0000 \u0000 \u0000 \u0000 We found evidence to support a causal relationship between smoking initiation and history of infertility in women.\u0000 \u0000 \u0000 \u0000 Several CVD risk factors are associated with history of infertility. Previous studies using Mendelian randomisation (MR) further support a causal relationship between BMI and infertility in women.\u0000 \u0000 \u0000 \u0000 We used data from the Trøndelag Health Study (HUNT) in Norway, a prospective population-based cohort study, including 26,811 women and 15,598 men participating in three survey collections in 1995-1997 (HUNT2), 2006-2008 (HUNT3) and 2017-2019 (HUNT4).\u0000 \u0000 \u0000 \u0000 Our outcome was women’s self-reported history of infertility, defined as ever having tried to conceive for 12 months or more or having used ART. We assigned the history of infertility reported by women to their male partners, therefore the measure of infertility was on the couple level. We used both conventional multivariable analyses and one-sample MR analyses to evaluate the association between female and male CVD risk factors (including BMI, blood pressure, lipid profile measurements, and smoking behaviours) and history of infertility in women and men, separately.\u0000 \u0000 \u0000 \u0000 A total of 4,702 women (18%) and 2,508 men (16%) were classified with history of infertility. We found a higher risk of infertility among female smokers compared to non-smokers in both multivariable and MR analyses (odds ratio [OR] in multivariable analysis, 1.20; 95% CI, 1.12-1.28; OR in MR analysis, 1.13; CI, 1.02-1.26), and potentially for higher BMI (OR in multivariable analysis, 1.13; CI, 1.09-1.18; OR in MR analysis, 1.11, CI, 0.92-1.34). In multivariable analysis in women, we also found evidence of associations between triglyceride levels, high-density lipoprotein cholesterol, lifetime smoking index, and smoking intensity with higher risk of infertility. However, these results were not consistent in MR analyses. We found no robust or consistent associations between male CVD risk factors and infertility.\u0000 \u0000 \u0000 \u0000 Our main limitation was that the CVD risk factors measured might not adequately capture the relevant time periods for when couples were trying to conceive. Additionally, we did not have information on causes of infertility in either women or men.\u0000 \u0000 \u0000 \u0000 Women with infertility could have a worse CVD risk factor profile and thus public health interventions aimed at reducing the impact of some CVD risk factors, such as smoking and BMI, could reduce the burden of infertility. However, additional MR studies of the relationship between CVD risk factors and infertility with a larger sample size would be of value.\u0000 \u0000 \u0000 \u0000 The study was supported by a grant from the European Research Council under the European Union’s Horizon 2020 research and innovation program (grant agreements No 947684). This research was also supported by the Research Council of Norway through its Ce","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141101525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of chemical <i>in vitro</i> activation versus fragmentation on human ovarian tissue and follicle growth in culture.","authors":"Jie Hao, Tianyi Li, Manuel Heinzelmann, Elisabeth Moussaud-Lamodière, Filipa Lebre, Kaarel Krjutškov, Anastasios Damdimopoulos, Catarina Arnelo, Karin Pettersson, Ernesto Alfaro-Moreno, Cecilia Lindskog, Majorie van Duursen, Pauliina Damdimopoulou","doi":"10.1093/hropen/hoae028","DOIUrl":"10.1093/hropen/hoae028","url":null,"abstract":"<p><strong>Study question: </strong>What is the effect of the chemical <i>in vitro</i> activation (cIVA) protocol compared with fragmentation only (Frag, also known as mechanical IVA) on gene expression, follicle activation and growth in human ovarian tissue <i>in vitro</i>?</p><p><strong>Summary answer: </strong>Although histological assessment shows that cIVA significantly increases follicle survival and growth compared to Frag, both protocols stimulate extensive and nearly identical transcriptomic changes in cultured tissue compared to freshly collected ovarian tissue, including marked changes in energy metabolism and inflammatory responses.</p><p><strong>What is known already: </strong>Treatments based on cIVA of the phosphatase and tensin homolog (PTEN)-phosphatidylinositol 3-kinase (PI3K) pathway in ovarian tissue followed by auto-transplantation have been administered to patients with refractory premature ovarian insufficiency (POI) and resulted in live births. However, comparable effects with mere tissue fragmentation have been shown, questioning the added value of chemical stimulation that could potentially activate oncogenic responses.</p><p><strong>Study design size duration: </strong>Fifty-nine ovarian cortical biopsies were obtained from consenting women undergoing elective caesarean section (C-section). The samples were fragmented for culture studies. Half of the fragments were exposed to bpV (HOpic)+740Y-P (Frag+cIVA group) during the first 24 h of culture, while the other half were cultured with medium only (Frag group). Subsequently, both groups were cultured with medium only for an additional 6 days. Tissue and media samples were collected for histological, transcriptomic, steroid hormone, and cytokine/chemokine analyses at various time points.</p><p><strong>Participants/materials setting methods: </strong>Effects on follicles were evaluated by counting and scoring serial sections stained with hematoxylin and eosin before and after the 7-day culture. Follicle function was assessed by quantification of steroids by ultra-performance liquid chromatography tandem-mass spectrometry at different time points. Cytokines and chemokines were measured by multiplex assay. Transcriptomic effects were measured by RNA-sequencing (RNA-seq) of the tissue after the initial 24-h culture. Selected differentially expressed genes (DEGs) were validated by quantitative PCR and immunofluorescence in cultured ovarian tissue as well as in KGN cell (human ovarian granulosa-like tumor cell line) culture experiments.</p><p><strong>Main results and the role of chance: </strong>Compared to the Frag group, the Frag+cIVA group exhibited a significantly higher follicle survival rate, increased numbers of secondary follicles, and larger follicle sizes. Additionally, the tissue in the Frag+cIVA group produced less dehydroepiandrosterone compared to Frag. Cytokine measurement showed a strong inflammatory response at the start of the culture in both groups. The RNA-s","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11128059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141158919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preconception depression reduces fertility: a couple-based prospective preconception cohort","authors":"Tierong Liao, Yaya Gao, Xinliu Yang, Yanlan Tang, Baoling Wang, Qianhui Yang, Xin Gao, Ying Tang, Kunjing He, Jing Shen, Shuangshuang Bao, Guixia Pan, Peng Zhu, Fangbiao Tao, S. Shao","doi":"10.1093/hropen/hoae032","DOIUrl":"https://doi.org/10.1093/hropen/hoae032","url":null,"abstract":"\u0000 \u0000 \u0000 Is preconception depression associated with time to pregnancy (TTP) and infertility?\u0000 \u0000 \u0000 \u0000 Couples with preconception depression needed a longer time to become pregnant and exhibited an increased risk of infertility.\u0000 \u0000 \u0000 \u0000 Preconception depression in women contributes to impaired fertility in clinical populations. However, evidence from the general population—especially based on couples—is relatively scant.\u0000 \u0000 \u0000 \u0000 A couple-based prospective preconception cohort study was performed in 16 premarital examination centers between April 2019 and June 2021. The final analysis included 16,521 couples who tried to conceive for ≤6 months at enrollment. Patients with infertility were defined as those with a TTP ≥12 months and those who conceived through ART.\u0000 \u0000 \u0000 \u0000 Couples’ depression was assessed using the Patient Health Questionnaire-9 at baseline. Reproductive outcomes were obtained via telephone at 6 and 12 months after enrollment. Fertility odds ratios (FORs) and infertility risk ratios (RRs) in different preconception depression groups were analysed using the Cox proportional-hazard models and logistic regression, respectively.\u0000 \u0000 \u0000 \u0000 Of the 16,521 couples analyzed, 10,834 (65.6%) and 746 (4.5%) couples achieved pregnancy within the first 6 months and between the 6th and 12th months, respectively. The median (P25, P75) TTP was 3.0 (2.0, 6.0) months. The infertility rate was 13.01%. After adjusting for potential confounders, in the individual-specific analyses, we found that preconception depression in women was significantly related to reduced odds of fertility (FOR = 0.947, 95% CI: 0.908–0.988), and preconception depression in either men or women was associated with an increased risk of infertility (women: RR = 1.212, 95%CI: 1.076–1.366; men: RR = 1.214, 95%CI: 1.068–1.381); in the couple-based analyses, we found that—compared to couples where neither partner had depression—the couples where both partners had depression exhibited reduced fertility (adjusted FOR = 0.904, 95%CI: 0.838–0.975). The risk of infertility in the group where only the woman had depression and both partners had depression increased by 17.8% (RR = 1.178, 95%CI: 1.026–1.353) and 46.9% (RR = 1.469, 95%CI: 1.203–1.793), respectively.\u0000 \u0000 \u0000 \u0000 Reporting and recall bias were unavoidable in this large epidemiological study. Some residual confounding factors—such as the use of anti-depressants and other medications, sexual habits, and prior depressive and anxiety symptoms—remain unaddressed. We used a cut-off score of 5 to define depression, which is lower than prior studies. Finally, we assessed depression only at baseline, therefore we could not detect effects of temporal changes in depression on fertility.\u0000 \u0000 \u0000 \u0000 This couple-based study indicated that preconception depression in individuals and couples negatively impacts couples’ fertility. Early detection and intervention of depression to improve fertility should focus on both sexes.\u0000 \u0000 \u0000 \u0000 This work was supported by grants f","PeriodicalId":73264,"journal":{"name":"Human reproduction open","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140966657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}