Food frontiers最新文献

{"title":"Medulla Tetrapanacis water extract ameliorates mastitis by suppressing bacterial internalization and inflammation via MAPKs signaling in vitro and in vivo","authors":"Carsten Tsun-Ka Kwok,&nbsp;Yuanhao Hu,&nbsp;Bun Tsoi,&nbsp;Fiona Wong,&nbsp;Pak-Ting Hau,&nbsp;Emily Wan-Ting Tam,&nbsp;Daniel Kam-Wah Mok,&nbsp;Yiu-Wa Kwan,&nbsp;George Pak-Heng Leung,&nbsp;Simon Ming-Yuen Lee,&nbsp;Jing-Jing Li,&nbsp;Franklin Wang-Ngai Chow,&nbsp;Sai-Wang Seto","doi":"10.1002/fft2.476","DOIUrl":"https://doi.org/10.1002/fft2.476","url":null,"abstract":"<p><i>Medulla Tetrapanacis</i> (MT) is a commonly used herbal ingredient in soup to promote lactation and management of mastitis among lactating mothers worldwide, particularly in Asian countries. However, scientific evidence to support its usage in the management of mastitis is limited. This study aimed to investigate the effects of MT water extract and its underlying mechanisms in <i>Staphylococcus aureus</i> (SA)–induced mastitis in human mammary epithelial cells (HuMEC) and lipopolysaccharide (LPS)–induced mastitis in lactating Sprague–Dawley (SD) rats. The anti-inflammatory and antibacterial effects of MT water extract were examined in SA-infected HuMEC by ELISA and plate counting, respectively. The effects of MT water extract on blood-milk barrier and the underlying mechanism of action of the MT water extract were also investigated by transendothelial electrical resistance assay and western blot. The effects and mechanisms of MT water extract on alleviating mastitis on SD rats were evaluated using LPS-induced mastitis rat model. Our results showed that MT water extract could suppress SA-induced mastitis by reducing SA internalization and growth, protecting blood-milk barrier integrity, and attenuating release of cytokines (interleukin 6 [IL-6] and tumor necrosis factor-alpha [TNF-α]) in HuEMC. Furthermore, the antibacterial effect might be related to the increase of antimicrobial peptides transcription, and the anti-inflammatory effect is at least partly mediated by inactivation of p38 and JNK signaling pathways. Finally, our in vivo results showed that MT water extract ameliorated LPS-induced mastitis in SD rats via suppressing inflammatory cytokine release (TNF-α, IL-6, and interleukin-1 beta), myeloperoxidase, and alleviating pathohistological damage by downregulation of JNK signaling pathway.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":"6 1","pages":"500-515"},"PeriodicalIF":7.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.476","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simulation of Transport Phenomena for Microwave Freeze-Drying of Potato Slices Using Finite Element Analysis","authors":"Narathip Sujinda,&nbsp;Jaturapatr Varith","doi":"10.1002/fft2.508","DOIUrl":"https://doi.org/10.1002/fft2.508","url":null,"abstract":"<p>This research intended to investigate the transport phenomena that occur during microwave freeze-drying (MFD) of potato slices using drying kinetics and finite element analysis (FEA). The impacts of microwave power levels and potato slice thickness on drying rate constant (<i>DR</i>) and average moisture diffusion (<i>D_AVG</i>) were analyzed using MFD kinetics and were then incorporated in the simulation. It was found that the <i>DR</i> and <i>D_AVG</i> were in the range of 301.3 × 10⁻³–775.4 × 10⁻³ min⁻¹ and 1.045 × 10⁻¹⁰–3.336 × 10⁻¹⁰ m²/s, respectively. In the sublimation phase, the <i>DR</i> and <i>D_AVG</i> were higher than those in the desorption phase. The <i>DR</i> and <i>D_AVG</i> increased as the microwave power level increased but decreased as the thickness increased. The FEA of temperature and moisture distribution within the potato slices demonstrated the outward transfer of heat and mass from the center to the exterior and closely matched the experimental data with an error margin of within 5%, leading to the proposed schematic shrinkage model corresponding to the MFD simulation.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":"6 1","pages":"532-548"},"PeriodicalIF":7.4,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.508","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143120600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allicin: A natural weapon against Taxol resistance in non-small cell lung cancer through Cathepsin B inhibition and lysosomal-autophagy disruption","authors":"Xudong Gao,&nbsp;Chang Xu,&nbsp;Ramesh Kumar Santhanam,&nbsp;Yingshi Zhang,&nbsp;Qingchun Zhao","doi":"10.1002/fft2.491","DOIUrl":"https://doi.org/10.1002/fft2.491","url":null,"abstract":"<p>Drug resistance in non-small cell lung cancer (NSCLC) limits its therapeutic efficacy. Allicin may help to solve the problem of Taxol resistance in NSCLC. This study aimed to explore the potential mechanism by which allicin reverses Taxol resistance in NSCLC. The potential mechanism of allicin reversing Taxol resistance in vitro was evaluated using cytotoxicity assays, evaluation of cell autophagy and lysosomal function. In addition, the in vivo model was established to evaluate the effectiveness of allicin in reversing Taxol resistance. The results showed that allicin effectively inhibited cell proliferation, induced apoptosis, and reversed Taxol resistance. It inhibits P-gp expression, reduces drug efflux, and disrupts cell autophagy. In particular, allicin inhibited Cathepsin B (CTSB), disrupted lysosomal function, blocked autophagy flux, reduced mitochondrial membrane potential, and enhanced the sensitivity to Taxol. Allicin combined with Taxol significantly inhibited the growth of tumor nodules and reduced their number, demonstrating its potential to reverse Taxol resistance in vivo. In summary, allicin reversed Taxol resistance by inhibiting P-gp and CTSB activity and disrupting the lysosomal-autophagy pathway. These findings highlight the potential of allicin for cancer treatment and drug development.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":"6 1","pages":"549-562"},"PeriodicalIF":7.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.491","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143118541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic amelioration of obesity in high-fat diet–fed mice by Bifidobacterium longum and green banana powder through targeting multiple pathways","authors":"Yi-Yao Lu,&nbsp;Zhi Jia,&nbsp;Meng-Yue Zhang,&nbsp;Juan Cheng,&nbsp;Ze-Xu Yu,&nbsp;Ye Li,&nbsp;Yi-Xuan Zhang,&nbsp;Xin Wu","doi":"10.1002/fft2.502","DOIUrl":"https://doi.org/10.1002/fft2.502","url":null,"abstract":"<p>Long-term consumption of high-fat diet (HFD) is a major significant risk factor for various diseases, including hyperlipidemia, diabetes, cardiovascular disease, and certain cancers. Recently, there has been a focus on effective dietary methods to improve lipid absorption and accumulation in the body. Simultaneously, exploring non-pharmacological interventions to counteract the adverse consequences of obesity is crucial. Probiotics and prebiotics emerge as promising alternatives in this context, with their combined utilization yielding substantial effects surpassing those achieved by individual application alone. The aim of this study was to investigate the combined effects of <i>Bifidobacterium longum</i> (BL) SYP-B4138 and green banana powder (GBP) on HFD-induced obesity while exploring their underlying mechanisms. The findings demonstrated that the combined intervention exhibited significantly greater efficacy than BL or GBP alone, including in mitigating weight gain and organ weight among obese mice, while also improving lipid profile, glucose tolerance, and inflammation levels. Furthermore, it effectively reduced liver fat accumulation. The mechanism study revealed that the combined intervention increased the expression of gene-related lipid metabolism, such as <i>Ppar</i>α, <i>Cpt1</i>a, <i>Fxr</i>, <i>Cyp7a1</i>, and <i>Bsep</i>, while decreasing expression of the regulatory genes involved in fat synthesis, <i>Acc1</i>, <i>Srebpf1</i>, and <i>Pparγ</i>. The correlation analysis of gut microbes and metabolites suggests that the combined effect of BL and GBP can reverse the intestinal microecological imbalance, leading to changes in bile acids, glycerophospholipids, and other related metabolites. Overall, this study demonstrates that BL combined with GBP can effectively alleviate dietary obesity, highlighting its potential as a non-pharmacological intervention for weight reduction.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":"6 1","pages":"485-499"},"PeriodicalIF":7.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.502","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143118393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Terpenoids from quinoa reverse chemoresistance in breast cancer by suppressing the stemness-related lncRNA 667012","authors":"Xiaxia Fan,&nbsp;Mangmang Feng,&nbsp;Dan Guo,&nbsp;Songtao Li,&nbsp;Yuxuan An,&nbsp;Jinmiao Tian,&nbsp;Zhuoyu Li","doi":"10.1002/fft2.503","DOIUrl":"https://doi.org/10.1002/fft2.503","url":null,"abstract":"<p>Chemoresistance is a major cause of breast cancer (BC) recurrence and metastasis, and chemotherapy resistance comes from tumor stemness. Our previous study showed that quinoa bran terpenoids (QBT) exhibited significant antitumor activity. In this study, we found that QBT increased the sensitivity of drug-resistant BC cells to the chemotherapeutic drugs adriamycin (ADR) and taxol (TAX). A novel lncRNA 667012 was identified along with QBT treatment. Moreover, overexpression of lncRNA 667012 promoted drug resistance, and the reduction of 667012 restored the drug sensitivity of resistant BC cells. More importantly, the findings revealed that lncRNA 667012 promoted stemness maintenance via the stemness-related Wnt pathway. Furthermore, the result showed that QBT reduced 667012 expression and inhibited the activation of β-catenin, thereby attenuating the stemness characteristics of BC and enhancing chemosensitivity. Consistently, the data were confirmed in the xenograft mice model. These results suggest that QBT could reverse chemoresistance by inhibiting stemness. It has the potential to be developed as a novel chemotherapeutic agent or chemosensitizer for BC treatment.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":"6 1","pages":"516-531"},"PeriodicalIF":7.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.503","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143118394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactiplantibacillus plantarum YY-112 ameliorates mouse immunosuppression by enhancing B/T-cell activation and maintaining Th1/Th2 homeostasis","authors":"Ying Yang,&nbsp;Mengfan Luo,&nbsp;Wanyi Zhou,&nbsp;Wenyang Tao,&nbsp;Jingrui Li,&nbsp;Jianrong Xing,&nbsp;Quanqin Tan,&nbsp;Yuxing Guo","doi":"10.1002/fft2.477","DOIUrl":"https://doi.org/10.1002/fft2.477","url":null,"abstract":"<p>In recent years, the development of immunosuppression has become common, and probiotics are a vital immunomodulatory strategy. This study evaluated the alleviating effect of <i>Lactiplantibacillus plantarum</i> (<i>L. plantarum</i>) YY-112 on cyclophosphamide-induced immune imbalance and resolved the possible mechanism of action. <i>L. plantarum</i> YY-112 at 10⁹ CFU/mL lowered loss of body weight and alleviated splenic injury, intestinal injury, and inflammation, according to the hematoxylin and eosin staining. Biochemical results showed that 10⁹ CFU/mL <i>L. plantarum</i> YY-112 increased interleukin-10 and immunoglobulin M levels and decreased interferon-γ levels. Additionally, the immunoregulatory effect at the molecular levels of <i>L. plantarum</i> YY-112 was analyzed by transcriptome sequencing. <i>L. plantarum</i> YY-112 significantly upregulated <i>Cr2</i> and downregulated <i>C5aR1</i> to regulate the complement system; downregulated <i>S100a8</i>, <i>S100a9</i>, and <i>Mmp9</i> to inhibit neutrophil aggregation; upregulated <i>Cd19</i>, <i>Cd72</i>, <i>Cd3e</i>, <i>Cd28</i>, <i>Cd80</i>, <i>Cd8a</i>, and histocompatibility-2-related genes to regulate B- and T-cell activation; and upregulated <i>Xiap</i>, <i>Malt1</i>, <i>Ikbkb</i>, <i>Mapk11</i>, <i>Mef2c</i>, <i>Mapk12</i>, <i>Ras</i>, and <i>Myc</i> to activate immune signaling pathways. Furthermore, <i>L. plantarum</i> YY-112 improved intestinal microbial structure and reduced enrichment of <i>Lachnospiraceae_NK4A136_group</i>, <i>norank_f_Lachnospiraceae</i>, <i>Desulfovibrio</i>, <i>Colidextribacter</i>, and <i>Helicobacter</i>. In conclusion, these findings offer valuable insights into the potential mechanisms through which <i>L. plantarum</i> YY-112 regulates immune imbalance.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":"6 1","pages":"465-484"},"PeriodicalIF":7.4,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.477","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143116014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma signature metabolites of dietary fat intake characterize associations with prevalent metabolic syndrome","authors":"Xuzhi Wan,&nbsp;Hongbo Shi,&nbsp;Wei Jia,&nbsp;Li Zhu,&nbsp;Yimei Tian,&nbsp;Denghui Meng,&nbsp;Anli Wang,&nbsp;Yaoran Li,&nbsp;Xiaohui Liu,&nbsp;Haoyu Li,&nbsp;Lange Zhang,&nbsp;Pan Zhuang,&nbsp;Yu Zhang,&nbsp;Jingjing Jiao","doi":"10.1002/fft2.505","DOIUrl":"https://doi.org/10.1002/fft2.505","url":null,"abstract":"<p>The metabolic response to dietary fat intake and its relation with metabolic syndrome (MetS) remains unclear. Here, we identified dietary fat related signature metabolites and characterized their associations with MetS prevalence. We enrolled 236 participants from the Precision Nutrition and Food Safety for Dietary Prevention of Chronic Disease study in China. Nontargeted metabolomics was conducted to investigate plasma metabolome. The signature metabolites in relation to dietary fat intake were assessed using elastic net regression with a 10-fold cross-validation. We identified multi-metabolite profiles comprising of 28, 19, 23, 31, and 25 metabolites, which were robustly correlated with dietary intake of saturated fatty acids, monounsaturated fatty acids, polyunsaturated fatty acids (PUFAs), <i>n </i>− 3 PUFAs, and <i>n </i>− 6 PUFAs, respectively (<i>r </i>= .47–.54; <i>p </i>&lt; .001). After adjustment for potential risk factors, metabolic signatures of PUFAs and <i>n </i>− 3 PUFAs were inversely associated with 31% and 48% lower MetS prevalence, respectively. Dietary intake of <i>n </i>− 3 and <i>n </i>− 6 PUFAs ameliorates key metabolites involved in the glyoxylate and dicarboxylate metabolism, arginine biosynthesis, and tricarboxylic acid cycle. Our findings revealed plasma metabolic signatures characterizing dietary fat intake and supported the beneficial role of PUFAs especially <i>n </i>− 3 PUFAs in MetS prevention.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":"6 1","pages":"435-447"},"PeriodicalIF":7.4,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.505","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143115687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial volatile organic compounds supplemented with light-emitting diode lighting enhance antioxidant capacity and delay senescence in broccoli","authors":"Taishan Huang,&nbsp;Junyan Shi,&nbsp;Xiaozhen Yue,&nbsp;Shuzhi Yuan,&nbsp;Xiangbin Xu,&nbsp;Jinhua Zuo,&nbsp;Qing Wang","doi":"10.1002/fft2.500","DOIUrl":"https://doi.org/10.1002/fft2.500","url":null,"abstract":"<p>Bacterial volatile organic compounds (BVOCs) are biological volatiles that can have a positive effect on the postharvest preservation of fruits and vegetables. In the present study, fumigation of broccoli with BVOCs released by <i>Lysinibacillus fusiformis</i> supplemented with light-emitting diode (LED) white light irradiation reduced the accumulation of reactive oxygen species in broccoli during senescence, enhanced the synthesis and accumulation of antioxidant substances, and increased antioxidant enzyme activity in stored broccoli. Compared to the control group (CK), transcriptomic and metabolic analyses of the composite treatment group (LED-BVOCs) on the 4 days of storage identified 3/1, 7/0, 1/1, 20/6, and 6/1 differentially expressed genes (DEGs)/differentially abundant metabolites in metabolic pathways related to ascorbic acid, glutathione, glucosinolate, polyphenols, and methionine, respectively, and there were 4 DEGs involved in the regulation of senescence during ethylene signal transduction. It is evident that LED-BVOCs treatment can effectively regulate the metabolic processes of antioxidants in broccoli during storage. Overall, the LED-BVOCs treatment improved the antioxidant capacity and reduced ethylene production in stored broccoli, thus delaying senescence. Our study provides new insights on the ability of using BVOCs as biopreservatives to improve the antioxidant capacity and delay senescence of broccoli during storage.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":"6 1","pages":"448-464"},"PeriodicalIF":7.4,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.500","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143116000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural insights and biological activities of flavonoids: Implications for novel applications","authors":"Sheng Tang,&nbsp;Botao Wang,&nbsp;Xin Liu,&nbsp;Wenxia Xi,&nbsp;Yuanyuan Yue,&nbsp;Xiang Tan,&nbsp;Junying Bai,&nbsp;Linhua Huang","doi":"10.1002/fft2.494","DOIUrl":"https://doi.org/10.1002/fft2.494","url":null,"abstract":"<p>Flavonoids are a major class of polyphenolic compounds that occur naturally in plants and are widely distributed in various dietary products. Flavonoids exhibit prominent physicochemical properties and biological activities, thereby garnering considerable attention. Notably, the physicochemical properties and functionalities of flavonoids are structurally dependent. Variations in chemical structure lead to differences in physicochemical characteristics among different flavonoid types. Structural alterations can modify these characteristics, influencing biological activities and mechanisms of action. However, these structural variations and their biological implication have not been comprehensively elucidated. In this review, we outline the structural characteristics of flavonoids, discuss biosynthesis, in vivo digestion, and absorption, and particularly emphasize the diverse biological activities and action mechanisms associated with different flavonoid structures. By summarizing the current scientific findings, we aim to elucidate the structure–activity relationships of flavonoids and provide a theoretical basis for the development of new flavonoid applications, which will help to target the design of functional molecules and moieties in the food and pharmaceutical industries for use in drug design and development.</p><p><b>Practical Application</b>: Understanding the structural characteristics and biological activities of flavonoids can guide the development of functional food products and dietary supplements with targeted health benefits. By leveraging the knowledge of flavonoid structure–activity relationships, food, and pharmaceutical industries can innovate formulations to enhance bioavailability and efficacy, potentially leading to the creation of tailored products for specific health conditions or dietary needs. This research lays the groundwork for the design of novel flavonoid-based interventions aimed at promoting human health and well-being.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":"6 1","pages":"218-247"},"PeriodicalIF":7.4,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143115688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Choline ameliorates tris (2-chloroisopropyl) phosphate-induced hepatocellular carcinoma metastasis by inhibiting ROS/Nrf2/Keap1-mediated autophagy","authors":"Chen Wang,&nbsp;Meng-chu Li,&nbsp;Si-yu Huang,&nbsp;Wen-ge Huang,&nbsp;Tong-tong He,&nbsp;Maierhaba Wusiman,&nbsp;Hui-lian Zhu,&nbsp;Zhao-yan Liu","doi":"10.1002/fft2.489","DOIUrl":"https://doi.org/10.1002/fft2.489","url":null,"abstract":"<p>Tris (2-chloroisopropyl) phosphate (TCPP) is an emerging environmental pollutant associated with liver diseases. However, its effects on hepatocellular carcinoma (HCC) remain unknown. Choline, a necessary dietary nutrient, has previously demonstrated inhibitory effects on HCC. Therefore, elucidating the underlying mechanism of TCPP exposure on HCC development and investigating whether choline could mitigate these effects may improve the prognosis of HCC patients. In this study, we examined the tumor-promoting effects of TCPP on HCC and explored the protective effects of choline. Our findings revealed that choline treatment attenuated the tumor-promoting effects of TCPP exposure on HCC cells’ epithelial-mesenchymal transition (EMT) and lung metastasis. Further investigation showed that TCPP exposure induced ROS production via NOX4 upregulation, while choline inhibited ROS generation, thereby mitigating the effects of TCPP on EMT and metastasis in HCC cells. Mechanistic analysis demonstrated that excessive ROS inhibited levels of Keap1, leading to upregulation and nuclear translocation of Nrf2, which promoted autophagy flux and accelerated EMT and metastasis of HCC cells. However, choline treatment significantly impaired TCPP-induced autophagy by attenuating the ROS/Nrf2/Keap1 pathway. Overall, our data illustrate the adverse effects of TCPP on the malignant progression of HCC and suggest that choline may serve as a potential nutrient to counteract the tumor-promoting effects of TCPP on HCC.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":"6 1","pages":"418-434"},"PeriodicalIF":7.4,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.489","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143115686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}