Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)最新文献

{"title":"THE STUDY OF THE RELATIONSHIP BETWEEN THE ACTIVITY OF EPIPHYSIS AND GONADS IN MALE RATS IN DIFFERENT SEASONS.","authors":"V Hnatiuk,&nbsp;N Kononenko","doi":"10.15407/fz62.06.095","DOIUrl":"https://doi.org/10.15407/fz62.06.095","url":null,"abstract":"<p><p>In male rats of different ages, we studied the functional activity of the pineal gland and the gonads during different seasons by determining the levels of melatonin and testosterone in the blood serum. It was found that the highest levels of melatonin observed in male rats in summer and winter, and the lowest - in autumn. The lowest level of melatonin has been established in the group of rats aged 9 months - 127.28+5.11 pmol/l that was 22% lower than the level of melatonin in 3 months old rats (P!0,05), and 20% below the level of melatonin in rats of 15 months old (Ps0,05). The highest level determined in rats aged 3 months (corresponds to the age of man -14 years old), the lowest - in rats 20 months-(corresponds to the age 55-56 years old). At the same time, the low level of melatonin was defined in rats aged 9 months in the autumn (corresponds to the age of man 29-30 years). In the study of testosterone levels in different seasons it was found that the highest level in all age groups presents in autumn, the lowest - in winter. When comparing the levels of testosterone in different age groups, it was determined that the fall of the highest level of testosterone present in the blood of males age 9 months and 15 months, which corresponds to the age of 29-30 and 43-44 of human years. Significant difference between the levels of testosterone in the groups that correspond to the reproductive age - rats of age 3, 9 and 15 months, are present only in the autumn; spring and summer - significant differences between the levels of testosterone absent. The degree of correlation differed in rats of different ages in different seasons: the highest correlation between the levels of melatonin and testosterone was determined in rats aged 9 months in autumn. The correlation coefficients in rats aged 20 months old have been weak - from 0.05 in the spring to 0.17 in autumn that shows the changes in neurohormonal regulation which occur with age.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 6","pages":"95-101"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40438304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[DEPENDENCE OF LEVEL OF MYELOPEROXIDASE OF PLASMA OF BLOOD ON FUNCTIONAL STATE OF NEUTROPHILS AT ACUTE INFARCT OF MYOCARDIUM.]","authors":"T I Gavrilenko,&nbsp;N O Ryzhkova,&nbsp;O M Parkhomenko","doi":"10.15407/fz62.04.060","DOIUrl":"https://doi.org/10.15407/fz62.04.060","url":null,"abstract":"<p><p>The purpose of this work was to define dependence of maintenance of myeloperoxidase (MPO) in plasma of blood of patients by the acute infarct of myocardium from the state of oxygen metabolism of neutrophils, which was estimated on activity of myeloperoxidases, superoxid-anion and catalase in cells and on maintenance by peroxigens. 19 is inspected practically healthy volunteers and 56 patients with the the acute infarct of myocardium. The got results testify that maintenance of MPO in plasma of blood of patients with acute infarct of myocardium depends on the functional state of neutrophils, which, in its turn, is related to the clinical features of patients. Low maintenance is associated exceptionally with low activity of MPO of neutrophils, and also other oxygen radicals. High maintenance of MPO in plasma accompanied low or high activity of MPO of neutrophils and is pathognomonic for patients with the acute infarct of myocardium.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 4","pages":"60-65"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36286922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SPECIFIC FEATURES OF CHRONORHYTH- MOLOGIC CHANGES OF THE ION-REGU- LATING FUNCTION OF THE KIDNEYS UN- DER THE HYPOFUNCTION OF THE PINEAL GLAND.","authors":"S B Semenenko,&nbsp;S S Tkachuk,&nbsp;O V Tkachuk,&nbsp;S Y Karateeva,&nbsp;V V Antsupova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The paper investigates the specific features of chronorhythmologic changes of the ion-regulating function of the kidneys under the hypofunction of the pineal gland. For the study of hypofunction of the pineal gland, animals were maintained under constant light stimulation during 7 days. It has been established that the hypofunction epiphysis point out disturbances of the phasic structure of the ion-regulating function in relation to chronograms of the intact group of rats. In terms of physiological pinealectomy observed in- crease in the excretion of sodium ions (6,11±0,81 mmol/h) and the concentration of this cation in the blood plasma remained elevated around the clock. The obtained results indicate significant changes of integral characteristics of the rhythms of electrolyte metabolism and the important role of the pineal gland in ensuring physiological homeostasis.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 5","pages":"45-49"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36482049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental therapy of graft-versus-host disease by mesenchymal stromal cells grown on oxide nanocoatings.","authors":"A N Goltsev,&nbsp;I V Rassokha,&nbsp;T G Dubrava,&nbsp;L V Ostankova,&nbsp;M V Ostankov,&nbsp;V I Safonov,&nbsp;A V Zykova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Immune aggression to transplanted allogeneic bone marrow, i.e. the graft-versus-host disease (GVHD), could be decreased by the suppression of effector and/or activation of T- regulatory cells (Treg). This task could be solved by co-transplantaiton of allogeneic bone marrow and mesenchymal stromal cells (MSCs). This study demonstrated the elevated immune modulating activity of MSCs by their culturing in vitro on Al₂0₃ oxide nanocoatings. Introduction of the cells to the animals with GVHD resulted in an increased content of Treg in the spleen of bone marrow recipients, reduced severity of the pathology, and higher survival of animals. Thefindings could be the basis for developing the new approaches to optimize the GVHD treatment methods involving the oxide nanocoating cultured MSCs.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 5","pages":"3-11"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36482587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[CORRECTION OF HEART RATE VARIABILITY USING DIAPHRAGMATIC BREATHING MODE BIOFEEDBACK IN HEALTHY PEOPLE.]","authors":"V P Feketa,&nbsp;L A Gleba,&nbsp;O S Palamarchuk,&nbsp;Ju M Savka,&nbsp;K B Kivezhdi","doi":"10.15407/fz62.04.066","DOIUrl":"https://doi.org/10.15407/fz62.04.066","url":null,"abstract":"<p><p>The research is an attempt to explain the use of portable computer devices aiming to correct heart rate variability (HRV) of healthy young men. According to the data, used deep diaphragmatic breathing in mode of biofeedback harmonizes breathing and blood circulation which positively affects the body's adaptive potential. However, developers present the portable instruments of biofeedback primarily as a means of psychological relaxation. The aim of this study is to determine whether such devices are able to significantly affect heart rate variability and how long this effect lasts. There were 30 males aged 18 to 20 years that participated in this study. They were randomly divided into three equal groups with up to 10 people in each of them. Members of the first group were doing breathing exercises using a portable biofeedback StressEraser device, the second group used Em-Wave2, and the third one used hardware and software system MyCalmBeat. Each session lasted from 10 to 15 minutes. There were 10 sessions held daily in the morning. HRV was recorded by remote heart rate monitor Polar RS800CX and software Polar ProTrainer5. 5-minutes' stationary segments of the curve chest electrocardiogram removal were used in order to calculate the HRV indexes. It was found that 15 minutes deep breathing in mode of biofeedback significantly affects the heart rate variability of healthy young people. In particular, it increases the overall heart rate variability according to the statistical and spectral analysis. It was established that in all 3 groups of experiment participants the spectral curve low-range \"Low frequency\" (LF) suffered the major shifts. These shifts reflect the activity level of the sympathetic autonomic nervous system (ANS) as well as vascular-motor center according to the conventional interpretation. It was found that all three groups suffered a specific weight decrease of Very Low Frequency (VLF)% respectively 11.8±2.2%;(p <0,01); 6.6 ± 2.8%; (p <0,02) and 6.8 ± 2.4% (p <0,05). This means that activity redistribution between different regulatory components of heart rate occurred in favour of the peripheral ANS parts. Heart rate variability changes of this kind remained valid for at least one month after the experiment. The greatest influence on heart rate variability was observed in the group of people who used the device StressEraser.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 4","pages":"66-75"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36286923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of 4-aminopyridine-induced neuronal activity and local pO(2)in rat hippocampal slices by changing the flow rate of the superfusion medium.","authors":"V G Sydorenko,&nbsp;O S Komarov,&nbsp;B S Sushko,&nbsp;A K Romanov,&nbsp;E V Isaeva,&nbsp;D S Isaev","doi":"10.15407/fz62.04.003","DOIUrl":"https://doi.org/10.15407/fz62.04.003","url":null,"abstract":"<p><p>The brain slice preparation is the most frequently used tool for testing of pharmacological agents on the neuronal excitability. However in the absence of blood circulation in vitro, the tissue oxygenation strongly depends on the experimental conditions. It is well established that both hypoxia as well as hyperoxia can modulate the neuronal network activity. Thereby changes in tissue oxygen level during experiment may affect the final result. In the present study we investigated the effect of oxygenation on seizure susceptibility in the hippocampal slice preparation using 4-aminopyridine (4-AP) model of ictogenesis in inmature rats. We found that changing the medium perfusion rate in the range of 1-5 ml/min greatly affects the tissue oxygenation, amplitude and frequency of 4-AP-induced synchronous neuronal activity. The decrease in the flow rate as well as substitution of the oxygen in the extracellular medium with nitrogen causes a strong reduction of 4-AP-induced synchronous neuronal discharges. Our results demonstrate a significant linear correlation between the power of 4-AP-induced neuronal activity and the oxygen level in slice tissue. Also we demonstrated that the presence of medium flow is a necessary condition to support the constant level of the slice oxygenation. These data suggest that the oxygen supply of the brain slice strongly depends on experimental protocol and could modulate in vitro neuronal network excitability which should be taken into consideration when planning epilepsy-related studies.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 4","pages":"3-11"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36286969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INFLUENCE OF HYPOXIC PRECONDITIONING ON THE MECHANISMS OF BLOOD OXYGEN TRANSPORT AND OXIDATIVE DAMAGES\u0000DURING HEPATIC ISCHEMIA-REPERFUSION IN RABBITS.","authors":"M N Khodosovsky","doi":"10.15407/fz62.03.039","DOIUrl":"https://doi.org/10.15407/fz62.03.039","url":null,"abstract":"<p><p>The effect of hypoxic preconditioning (HP) on the blood\u0000oxygen-binding properties and liver oxidative damages was\u0000determine during hepatic ischemia-reperfusion (HIR) in rabbits.\u0000Animals were randomized into two experimental groups:\u00001st (HIR) - hepatic ischemia (30 min by Pringle maneuver) and\u0000reperfusion (120 min); 2nd (HP+HIR) – before HIR the rabbits\u0000were passed through hypoxic chamber at 3500 m altitude during\u00001 hr/day (3 times day after day). The parameters of blood\u0000oxygen transport (р50, рСО2, рО2, рН, HCO3\u0000-, ABE and ect.),\u0000lipid peroxidation products (conjugated dienes, Schiff bases)\u0000and blood hepatic markers (ALT, AST) were detected. It’s\u0000found that HIR leads to decline in hemoglobin oxygen affinity,\u0000activation of lipid peroxidation processes and elevation of ALT\u0000and AST activities in the 1st group. Hypoxic preconditioning\u0000(2nd group) markedly increased hemoglobin oxygen affinity,\u0000reduced the lipid peroxidation processes and ALT and AST\u0000activities in the blood during HIR. Thus, HP has a protective\u0000effect during HIR through elevation of hemoglobin oxygen\u0000affinity and declining hepatic oxidative damages.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 3","pages":"39-47"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35939520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PECULIARITIES OF THE INFLUENCE OF STREPTOZOTOCIN-INDUCED DIABETIS AND INCOMPLETE ISCHEMIAREPERFUSION OF THE BRAIN DURING APOPTOSIS OF VARIOUS NEOCORTICAL LOBES OF RATS.","authors":"T M Boychuk,&nbsp;T I Kmet","doi":"10.15407/fz62.02.072","DOIUrl":"https://doi.org/10.15407/fz62.02.072","url":null,"abstract":"<p><p>The effect of diabetes mellitus on the dynamics of neurocyte and gliacyte apoptosis intensity in the cortex of the frontal, parietal and temporal lobes of the cerebral hemispheres under conditions of ischemic-reperfusion lesion has been studied in experiments on rats. The level of apoptotic processes in the neuro- and gliacytes of the frontal cortex has been found to be unchanged after 20 minutes of carotid ischemia followed by one hour reperfusion according to the indices examined in animals with out diabetes mellitus. Apoptosis of neurocytes is activated in the cortex of the parietal lobe, and that of the neuro- and gliacytes – in the cortex of the temporal lobe. Three-month diabetes mellitus intensifies apoptosis of neurons and glial cells in the cortex of the frontal and temporal lobes, neurons in the cortex of the parietal lobe and decreases apoptosis of gliacytes in it. In early ischemic-reperfusion period the activity of apoptotic processes in the cortex of the frontal and temporal lobes does not change in animals with diabetes mellitus, but it decreases in the cortex of the parietal lobe at the expense of glial cells. On the 12th day of observation the activity of apoptotic processes in neurocytes of the cortex of the temporal lobe increases in rats without diabetes mellitus, and it decreases in the glial cells. We detected a reduced content of the protein p53 in neurons and increased density of р53+-cells. In this period of observation in rats with diabetes mellitus the activity of apoptotic processes decreases in general both in neurons and glial cells of all the lobes. The results obtained point for the availability of regional differences in the dynamics of reaction of the cerebral hemisphere lobes in response to ischemic-reperfusion injury charachterized by the intensity of apoptosis of neurons and glial cells. The results also point for modifying effect of diabetes mellitus on the indices studied.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 2","pages":"72-8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35910900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FEATURES OF LIMBIC-NEOCORTICAL AND MONOAMINERGIC CORRELATIONS IN THE DEVELOPMENT OF WITHDRAWAL\u0000SYNDROME OF INHALATIONS OF VAPORS OF ORGANIC SOLVENT «646» IN RATS.","authors":"N A Levicheva,&nbsp;A M Titkova","doi":"10.15407/fz62.03.060","DOIUrl":"https://doi.org/10.15407/fz62.03.060","url":null,"abstract":"<p><p>The aim of the work was to study the limbic-neocortical and\u0000monoaminergic features of the development of withdrawal\u0000syndrome of volatile organic compounds (VOC) inhalations\u0000in rats. The study was carried out in 30 three months old\u0000male rats with dependence on inhalations of organic solvent\u0000“646” which contains up to 50 % mix of toluene and acetone.\u0000It has been shown that the withdrawal syndrome of inhalant\u0000is characterized by increased excitability and behavioral\u0000manifestations of equivalents of convulsive reactions such as\u0000oral hyperkinesis, head shaking and changes in the frequencyamplitude\u0000spectrum of the biopotentials in structures of the\u0000brain limbic-neocortical system with the initiation in the medial\u0000olfactory region and hippocampus. At the hypothalamus level,\u0000withdrawal of VOC inhalations produces the depleting impact\u0000on the catecholaminergic structures with a stronger effect in\u0000neuronal endings with adrenaline as neurotransmitter. Withdrawal\u0000syndrome evokes a significant decrease in dopamine\u0000content by 61 %, noradrenaline by 77 % and adrenaline by 92\u0000% in the hypothalamus and increase in serotonin concentration\u0000in blood serum by 16 % in rats with initial preference to\u0000inhalations of organic solvent “646”. In rats with the absence\u0000of initial preference to inhalations of organic solvent “646”\u0000a decrease in adrenaline level in the hypothalamus by 77 %\u0000was detected.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 3","pages":"60-5"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35939522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE SECURITY AND CRITICAL LEVELS OF PHYSICAL ACTIVITY FOR TRAINED AND UNTRAINED PERSONS IN MUSCLE PERFORMANCE POWER ORIENTATION.","authors":"A A Chernozub","doi":"10.15407/fz62.02.110","DOIUrl":"https://doi.org/10.15407/fz62.02.110","url":null,"abstract":"<p><p>The paper studied the problem of finding the optimal methodology for determining the safety and critical load conditions for the body trained and untrained people in the process of training the force pool. It was found that for trained individuals who have a sufficient level of adaptation to the power load, the use of “standard” modes of operation in the course of employment by fitness power safely. However, the problematic issue is that to the untrained troops, it is almost impossible to determine the safe load parameters using conventional techniques, which are mainly used in cyclic sports. Thus, the solution to this problem lies in the fact that using the integral method for estimating the value of power loads and a computer program for determining the relative weight of the index weights, safety and critical load parameters were calculated for untrained persons. At the same time, studied the biochemical parameters of blood (testosterone, cortisol, and lactate dehydrogenase activity) fixed after exercise, compared with the state of rest, confirmed our calculations clearly enough.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 2","pages":"110-6"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35911856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}