Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)最新文献

{"title":"[INFLUENCE OF INTERMITTENT NORMOBARIC HYPOXIA ON CARDIOVASCULAR SYSTEM AND HEMODYNAMIC PARAMETERS OF CHILDREN LIVED AT RADIATION CONTAMINATED TERRITORIES.]","authors":"L M Lisukha,&nbsp;V Ya Berezovsky","doi":"10.15407/fz62.04.046","DOIUrl":"https://doi.org/10.15407/fz62.04.046","url":null,"abstract":"<p><p>The effect of intermittent normobaric hypoxia (INH) of sanogenic level on the state of the cardiovascular system in 120 children aged from 12 to 17 years was studied. Children were divided into 3 groups. The 1st control group consisted of 20 persons (practically healthy ones), the 2nd group, a group of comparison, included 50 persons, and there were 50 persons in the 3rd group - a main group. Examined patients of two studied groups received the standard therapy, and those of the 3rd group - the seances of intermittent, normobaric hypoxia additionally to standard therapy. Systolic arterial pressure was significantly decreased in the rest from 108,2±1,9 to 103,8±1,1 mm Hg in children of the main group. Comparing the heart rate, systolic arterial pressure and diastolic arterial pressure their statistically decreased values by 9,3 min(-1), 8,36 and 7,84 mm Hg were revealed at 10 minutes of orthostatic, respectively. Autonomic supplying the activity of cardiovascular system with normal type of reaction was 34 % higher than that of the comparison group. It indicates an the improvement in hemodynamics and an increase in adaptation capabilities of the autonomic nervous system.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 4","pages":"46-52"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36286468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[REDUCED RESPONSE OF NATURAL KILLER LYMPHOCYTES TO TOLL-LIKE RECEPTOR 3 STIMULATION IN CHILDREN WITH RECURRENT INFECTIONS.]","authors":"D V Osypchuk,&nbsp;V P Chernyshov,&nbsp;L I Chernysheva,&nbsp;N P Kissel,&nbsp;B V Dons'koi,&nbsp;I M Matvienko,&nbsp;V P Rodionov,&nbsp;Y Ua Makovs'ka","doi":"10.15407/fz62.04.012","DOIUrl":"https://doi.org/10.15407/fz62.04.012","url":null,"abstract":"<p><p>In this study, we examined the activation of natural killer(NK)-lymphocytes mediated by Toll-like receptor 3(TLR3), in the group of children with recurrent infections and the group of children with invasive bacterial infections. We examined level of CD69 (marker, of activation) expression on NK - lymphocytes after incubation with TLR3 ligand. There was a significant decrease in the level of an activation marker - 36,3±4,4% in the group of children with recurrent infections, compared with a control group of healthy children (56,5±4,9%) and the group of children with invasive bacterial infections (55,9±4,4%). Also, decreased was an activation potential of NK-lymphocytes - 24,9±L4,5% which was calculated as the difference between the percentage of CD69+ NK - lymphocytes after incubation and spontaneous value (without the addition of activator). There were no correlations between markers of TLR3 mediated activation of NK-cells and the age. Thus, the ability to activate NK - lymphocyte mediated by TLR3, independent of age and reduced in the group of children with recurrent infections. Reduced activation of NK-lymphocytes may contribute to increased susceptibility to viral infections and bacterial complications.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 4","pages":"12-17"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36286971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SINGLE-CHANNEL ION CURRENTS IN THE NUCLEAR ENVELOPE OF RAT CARDIOMYOCYTES.","authors":"O A Kotyk,&nbsp;A B Kotliarova,&nbsp;A O Polishchuk,&nbsp;S M Marchenko","doi":"10.15407/fz62.06.003","DOIUrl":"https://doi.org/10.15407/fz62.06.003","url":null,"abstract":"<p><p>Using the patch clamp technique in nucleus attached configuration we have found that the nuclear membrane of rat cardiomyocytes contains different types of ion channels with conductances in the range from 10 to 400 pS. In particular, we recorded inositol 1,4,5-trisphosphate receptors with conductance of 384 ± 5 pS and 209 ± 13 pS cation channels similar to LCC-channels, previously reported in neurons. In addition, we found at least two types of ion channels with significantly higher conductance than that of LCC-channels and several types of ion channels with lower conductance (10-90 pS).</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 6","pages":"3-8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40437796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ASSESSMENT OF FUNCTIONAL CHANGES TEAR PRODUCTION UNDER THE ACTION OF THE EYE DROPS ON THE BASE OF NATURAL MOLECULE OF ECTOINE AND ARTIFICIAL TEARS IN PATIENTS WITH DRY EYE SYNDROME ON THE BACKGROUND OF ENDOCRINE OPHTHALMOPATHY.","authors":"N N Veselovskaya,&nbsp;I B Zherebko","doi":"10.15407/fz62.06.118","DOIUrl":"https://doi.org/10.15407/fz62.06.118","url":null,"abstract":"<p><p>Conducted a comparative analysis of functional changes in tear production in patients with dry eye syndrome and endocrine ophthalmopathy in the conditions of the long-term acting of preservative free medications based on natural substances. A total of 30 people, aged 35 to 53 years old with clinical manifestations of DES on the background of EO were divided on two groups. In I group eye drops of ectoine and in II - artificial tears were administered. The examination included general and specific methods. The term of follow up - 30 days. It was found that long-term use of preservative free eye drops based on ectoine leads to more expressive positive changes in the condition of the anterior surface of the eye and the secretion and quality of the tear.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 6","pages":"118-121"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40526531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reparative processes in jaw bones under using of different plastic materials.","authors":"Kh R Pohranychna","doi":"10.15407/fz62.06.110","DOIUrl":"https://doi.org/10.15407/fz62.06.110","url":null,"abstract":"<p><p>The comparative experimental study of osseous tissue regeneration in the createdforaminous mandibular defects being implanted with bioceramics Kergap T-300 and osteoplastic material Osteopor, as well as justifying the possibility ofusing the developed reparative material in the maxillofacial surgery clinics based on the analysis ofhistomorphological research results for are the paper objectives. 78 nonlinearwhite male rats weighing 240-300 g withforaminous mandibular defects were used as models for studying impact on the reparative osteogenesis processes made by the bonegraft material. Reparative changes in the implantation sites were studied in real time with the help of histological drugs under the optical microscopy. The graft components were the morphometry object calculated at the percentage ratio per the graft space unit in three cuts from each section. In 27.7% cases the rats'mandibular defects repair under the natural environment conditions of osseous tissue regeneration achieved with a blood clot was complicated by purulence, the surgical wound dehiscence with pyorrhea or perimandibular abscess. In other cases regeneration caused the development of heterogeneous graft made of chondroid-fibroblastic and osteoid tissues of different organization levels. The comparative experimental morphological and histomorphometric studies of the bone regeneration involving the replacement of created jaw defects with osteoplastic material Kergap-T alone and- in the combination with the lyophilized biological placenta implant Osteopor proved that the latter speeded up the beginning of active regenerative processes promoting the early defect filling with the neogenic organotypic osseous tissue comprising 52(43-63)[46-58]% and 74(57-85)[64-79]% of the bone graft in 60 and 90 days after the surgical intervention correspondingly (being equal to 24(13-29)[20-28]% i 32(27-38)[30-34]% correspondingly in cases when Kergap-Twas applied. According to the morphometric researchfindings, in cases of Osteopor and Kergap-T application the tabular bone element ofthe graft was equal to 53 (43-60) [46-561% and 15(13-18) [14-16]% correspondingly.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 6","pages":"110-117"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40537950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE EFFECT OF GADOLINIUM ORTHOVANADATE NANOPARTICLES BY NEONATAL INDUCED REPRODUCTIVE DISEASE IN MALE RATS.","authors":"I O Belkina,&nbsp;N P Smolenko,&nbsp;V K Klochkov,&nbsp;Yu V Malukin,&nbsp;E E Chistyakova,&nbsp;N A Karpenko,&nbsp;Yu I Karachentsev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>For the purpose to develop of method for treatment of male hypofertility the efficiency of gadolinium orthovanadate nanoparticles (NP GdVO4) in rats with neonatal induced reproductive disease, has been investigated. The progeny of intact rats parents were exposed to emotional stress (Maternal separation stressn) and received an excessive amount of the blend of phytoestrogens (Ph) with mother's milk (1st group) from 3 to 22 day life. At the age of 10 months males received NP GdVO4 (0.33 mg/kg; 2nd group) or speman (158 mg/kg; 3rd group) with feed over the 70 days. Sexual behavior and fertility of males were investigated; the integrated indicator of reproductive potential (FI) was calculate and was estimated with the data of animals from group Control who were given vehicle (water). In the 1st group sexual behavior of males was characterized by accelerating sexual responses on female (double shortening of mounting and intromission latency) and a reduced ability to achieve ejaculation and to start the second round of copulations. The less females have been inseminated by these males (67% vs. 91% in Control group), but almost all became pregnant. As a result of the neonatal induction deterioration in the quality of male gametes intrauterine pregnancy loss increased more than twice, and the total number of foetus decreased (on 21%). These changes have led to a reduction of the male integrated indicator of reproductive potential (Fi) in 2.6 times compared with control animals. Using a NP GdVO4 rehabilitated male sexual behaviour indicators to Control group level, resulting in an increase in the number of fertilized females (up to 95%). In pregnant females decreased fetal losses (up to 15.2% vs. 18.1% in control, p<0,05) and increase in the number of fetuses (on 25%, p<0,05) that shows an improvement in the quality of sperm. The integrated indicator of reproductive potential (F1) increased to (8.3 ± 0.5) vs. (3.0 ± 0.3) u. in the 1st group (without treatment), that statistically higher than in groups Control and 3rd. Application of NP GdVO4 in rats with neonatal inductive pathology of the reproductive function and metabolic disorders normalizes sexual behaviour, the quality of sperm that restores male fertility and improves reproductive potential much more efficiently than the reference drug speman.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 5","pages":"76-82"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36482056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE LEVEL OF GENERAL IMMUNOGLOBULIN E (IGE) IN RATS OF DIFFERENT AGE WITH ATOPIC DERMATITIS.","authors":"A I Bereznyakova,&nbsp;O D Jemela,&nbsp;V F Cheremisina","doi":"10.15407/fz62.01.092","DOIUrl":"https://doi.org/10.15407/fz62.01.092","url":null,"abstract":"<p><p>In the article we studied the level of general immunoglobulin E in rats of different age with atopic dermatitis. On the basis of the obtained results characteristics are given of levels the general IgE in rats of different age and it’s increasing with age. In rats which had been experimentally reproduced atopic dermatitis the average value of general IgE was increased in prepubertal rats, mature and old animals significantly (р<0,001). That can have a diagnostic and predictive value of this indicator at atopic dermatitis.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 1","pages":"92-4"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35910470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OMEGA-3 POLYUNSATURATED FATTY ACIDS NORMALIZE THE FUNCTION OF MITOCHONDRIA, ACTIVITY OF ENZYMES OF PROOXIDANT-ANTIOXIDANT SYSTEM AND THE EXPRESSION OF CYTOCHROME Р450 2Е1 AFTER ISOPROTERENOLINDUCED MYOCARDIAL INJURY.","authors":"O S Panasiuk,&nbsp;A M Shysh,&nbsp;O O Moibenko","doi":"10.15407/fz62.02.064","DOIUrl":"https://doi.org/10.15407/fz62.02.064","url":null,"abstract":"<p><p>We have studied the influence of dietary ω-3 polyunsaturated fatty acids (ω-3 PUFA) on the functioning of subsarcolemmal and interfibrillar mitochondrial fractions of rat myocardium, changes in expression of cytochrome P450 (CYP2E1) and the activity of enzymes of prooxidant-antioxidant system after isoproterenol-induced myocardial injury. It has been found that in vivo administration of ω-3 PUFA (Epadol 0.1 ml/100 gr of weight for 4 weeks) significantly reduced the swelling of subsarcolemmal and interfibrillar mitochondrial fractions by 65.52% 54.84% respectively, pointing for a decrease of damage of the mitochondrial function evoked by in vivo administration of isoproterenol. In vivo administration of ω-3 PUFAs prevents a decrease in the activity of antioxidant enzymes catalase and superoxide dismutase (2.65 and 7.1- fold, respectively) after isoproterenol-induced myocardial injury. We suggest that the development of oxidative stress after isoproterenol-induced myocardial injury can be caused by a significant increase in the expression of cytochrome P450 2E1 (73.3%), and administration of ω-3 PUFAs prevents such changes.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 2","pages":"64-71"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35910899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HEART RATE REGULATION DURING ADAPTATION TO CONDITIONS IN ANTARCTICA.","authors":"V V Kalnish,&nbsp;G Yu Pyshnov,&nbsp;E V Моisеyenko,&nbsp;V V Оpanasenko,&nbsp;L M Аlekseyeva,&nbsp;L G Vysotska","doi":"10.15407/fz62.03.020","DOIUrl":"https://doi.org/10.15407/fz62.03.020","url":null,"abstract":"<p><p>The article deals with topical issues of the problem of studying\u0000the mechanisms of human adaptation to Аntarctic conditions,\u0000in particular, the characteristics of the restructuring regulation\u0000of cardiovascular system during expedition activity at\u0000the Ukrainian Antarctic Station «Academic Vernadsky». The\u0000studies were performed directly in Antarctica with the participation\u0000of winterers, who during a year were surveyed monthly\u0000by cardiointervalography and ECG. As a result of analysis of\u0000variance of heart rate in Antarctic conditions we identified the\u0000phasic restructuring of the regulation of heart rhythm of winterers.\u0000The findings extend the understanding of the peculiarities\u0000of realization of regulating the circulatory system during the\u0000change of adaptation phases. In particular, the RRNN index\u0000has a phasic structure and ranges ± 7-9 c.u. from the beginning\u0000of wintering, and in the last phase is increased to -32 c.u. The\u0000activity of the development of stress condition among winterers\u0000reflects the dynamics of changes in the rate ІNt function,\u0000which from the beginning of wintering till January ranged\u0000from 2-5 c.u. by modulo, and in the months before the return\u0000(February, March), the rate reached 46 c.u. It is established a\u0000dominant role of the time factor in influencing the quality adjustment\u0000of adaptation processes. While during the first half of\u0000the expedition heart rate variability had a balanced regulatory\u0000influence, at the end of the expedition an increased activity\u0000of the sympathetic division of the autonomic nervous system\u0000and stress regulatory mechanisms were observed. This may\u0000indicate for incomplete adaptation that requires the application\u0000of methods of correction of functional state.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 3","pages":"20-9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35939518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PROSPECTS OF C60 FULLERENE APPLICATION AS A MEAN OF PREVENTION AND CORRECTION OF ISCHEMIC-REPERFUSION INJURY\u0000IN THE SKELETAL MUSCLE TISSUE.","authors":"S Yu Zay,&nbsp;D A Zavodovskyi,&nbsp;K I Bogutska,&nbsp;D N Nozdrenko,&nbsp;Yu I Prylutskyy","doi":"10.15407/fz62.03.066","DOIUrl":"https://doi.org/10.15407/fz62.03.066","url":null,"abstract":"<p><p>lack of accurate diagnostic tests for the rapid detection of\u0000ischemic injury remains an urgent problem. In this context,\u0000a search for specific markers of ischemia as well as new\u0000therapeutic agents for prevention and treatment of ischemic\u0000injury continues. Water-soluble nano-sized C60 fullerenes, as\u0000powerful antioxidants, can act as promising means for correction\u0000of various muscle system states, in the base of which lie\u0000the destructive effects of free radicals.</p>","PeriodicalId":73031,"journal":{"name":"Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)","volume":"62 3","pages":"66-77"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35940407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}