Development & reproduction最新文献_第5页

Relationship between Condition Index Values and Expression Levels of Gene and Protein in the Adductor Muscle of Diploid and Triploid Oysters Crassostrea gigas. 二倍体和三倍体牡蛎内收肌基因和蛋白表达量与状态指数值的关系。

Development & reproduction Pub Date : 2022-12-01 DOI: 10.12717/DR.2022.26.4.165

Su-Jin Park, Youn Hee Choi

{"title":"Relationship between Condition Index Values and Expression Levels of Gene and Protein in the Adductor Muscle of Diploid and Triploid Oysters Crassostrea gigas.","authors":"Su-Jin Park, Youn Hee Choi","doi":"10.12717/DR.2022.26.4.165","DOIUrl":"https://doi.org/10.12717/DR.2022.26.4.165","url":null,"abstract":"Three proteins [myosin heavy chain (MHC), filamin-C fragment (FIL-C), and actin 2 (ACT2)] were identified in adductor muscle from diploid and triploid Pacific oysters (Crassostrea gigas) and the relationship between the condition index (CI) and mRNA expression of these genes was investigated, together with the mRNA expression of molluscan insulin-related peptide (MIP), C. gigas insulin receptor-related receptor (CIR), and insulin-like growth factor binding protein complex acid labile subunit (IGFBP-ALS). Monthly changes in the CI were similar to the changes in the tissue weight rate in both groups. ACT2 and MHC mRNA expression was statistically higher in the triploid than the diploid, while FIL-C mRNA expression was significantly higher in the diploid (p<0.05). The MIP, CIR, and IGFBP-ALS mRNA expression of the diploid oysters were all significantly higher in July than in other months (p<0.05). The MIP, CIR, and IGFBP-ALS mRNA expression in the triploid oysters was high in July, but there were no significant differences (p>0.05). Changes in the expression levels of the genes investigated in this study could be used as intrinsic indicators of the annual growth, maturity, and spawning period of cultured diploid and triploid C. gigas in Tongyeong, Korea.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"26 4","pages":"165-174"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b9/98/dr-26-4-165.PMC9925188.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Pluripotent Stem Cell-Derived Alveolar Epithelial Cells as a Tool to Assess Cytotoxicity of Particulate Matter and Cigarette Smoke Extract. 人类多能干细胞衍生的肺泡上皮细胞作为评估颗粒物质和香烟烟雾提取物细胞毒性的工具。

Development & reproduction Pub Date : 2022-12-01 DOI: 10.12717/DR.2022.26.4.155

Jung-Hyun Kim, Minje Kang, Ji-Hye Jung, Seung-Joon Lee, Seok-Ho Hong

{"title":"Human Pluripotent Stem Cell-Derived Alveolar Epithelial Cells as a Tool to Assess Cytotoxicity of Particulate Matter and Cigarette Smoke Extract.","authors":"Jung-Hyun Kim, Minje Kang, Ji-Hye Jung, Seung-Joon Lee, Seok-Ho Hong","doi":"10.12717/DR.2022.26.4.155","DOIUrl":"https://doi.org/10.12717/DR.2022.26.4.155","url":null,"abstract":"Human pluripotent stem cells (hPSCs) can give rise to a vast array of differentiated derivatives, which have gained great attention in the field of in vitro toxicity evaluation. We have previously demonstrated that hPSC-derived alveolar epithelial cells (AECs) are phenotypically and functionally similar to primary AECs and could be more biologically relevant alternatives for assessing the potential toxic materials including in fine dust and cigarette smoking. Therefore, in this study, we employed hPSC-AECs to evaluate their responses to exposure of various concentrations of diesel particulate matter (dPM), cigarette smoke extract (CSE) and nicotine for 48 hrs in terms of cell death, inflammation, and oxidative stress. We found that all of these toxic materials significantly upregulated the transcription of pro-inflammatory cytokines such as IL-1α, IL-β, IL-6, and TNF-α. Furthermore, the exposure of dPM (100 μg/mL) strongly induced upregulation of genes related with cell death, inflammation, and oxidative stress compared with other concentrations of CSE and nicotine. These results suggest that hPSC-AECs could be a robust in vitro platform to evaluate pulmotoxicity of various air pollutants and harmful chemicals.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"26 4","pages":"155-163"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/73/c7/dr-26-4-155.PMC9925186.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10825155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Che-Yong() Logic and Research Methodology. 智勇()逻辑和研究方法论。

Development & reproduction Pub Date : 2022-12-01 DOI: 10.12717/DR.2022.26.4.183

YongNam Yun

引用次数: 0

Expression of Kisspeptin in the Adult Hamster Testis Kisspeptin在成年仓鼠睾丸中的表达

Development & reproduction Pub Date : 2022-09-01 DOI: 10.12717/DR.2022.26.3.107

Jin-Soo Park, Y. Cheon, D. Choi, Sung-Ho Lee

{"title":"Expression of Kisspeptin in the Adult Hamster Testis","authors":"Jin-Soo Park, Y. Cheon, D. Choi, Sung-Ho Lee","doi":"10.12717/DR.2022.26.3.107","DOIUrl":"https://doi.org/10.12717/DR.2022.26.3.107","url":null,"abstract":"Abstract Kisspeptins, products of KISS1 gene, are ligands of the G-protein coupled receptor (GPR54), and the kisspeptin-GPR54 signaling has an important role as an upstream regulator of gonadotropin releasing hormone (GnRH) neurons. Interestingly, extrahypothalamic expressions of kisspeptin/GPR-54 in gonads have been found in primates and experimental rodents such as rats and mice. Hamsters, another potent experimental rodent, also have a kisspeptin-GPR54 system in their ovaries. The presence of testicular kisspeptin-GPR54 system, however, remains to be solved. The present study was undertaken to determine whether the kisspeptin is expressed in hamster testis. To do this, reverse transcription-polymerase chain reactions (RT-PCRs) and immunohistochemistry (IHC) were employed. After the nest PCR, two cDNA products (320 and 280 bp, respectively) were detected by 3% agarose gel electrophoresis, and sequencing analysis revealed that the 320 bp product was correctly amplified from hamster kisspeptin cDNA. Modest immunoreactive (IR) kisspeptins were detected in Leydig-interstitial cells, and the weak signals were detected in germ cells, mostly in round spermatids and residual bodies of elongated spermatids. In the present study, we found the kisspeptin expression in the testis of Syrian hamster. Further studies on the local role(s) of testicular kisspeptin are expected for a better understanding the physiology of hamster testis, including photoperiodic gonadal regression specifically occurred in hamster gonads.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"26 1","pages":"107 - 115"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45938089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Distances of Binary Pen Shell Atrina pectinata Populations 二元笔壳刺鼠种群的遗传距离

Development & reproduction Pub Date : 2022-09-01 DOI: 10.12717/DR.2022.26.3.127

Jong-Man Yoon

引用次数: 0

Transforming Stimulated Clone 22 (TSC-22) Interacts Directly with Bromodomain-Containing Protein 7 (BRD7) to Enhance the Inhibition of Extracellular Signal-Regulate Kinase (ERK) Pathway in Ovarian Cancer 转化刺激克隆22（TSC-22）与含溴代胺蛋白7（BRD7）直接相互作用增强对卵巢癌症细胞外信号调节激酶（ERK）通路的抑制作用

Development & reproduction Pub Date : 2022-09-01 DOI: 10.12717/DR.2022.26.3.117

Seung-Hoon Lee, D. Choi

引用次数: 0

Styrene Cytotoxicity in Testicular Leydig Cells In Vitro 体外睾丸间质细胞的细胞毒性研究

Development & reproduction Pub Date : 2022-09-01 DOI: 10.12717/DR.2022.26.3.99

Jin-Yong Chung, J. Park, Y. Kim, Seung-Jin Lee, Wook-Joon Yu, Jong-Min Kim

{"title":"Styrene Cytotoxicity in Testicular Leydig Cells In Vitro","authors":"Jin-Yong Chung, J. Park, Y. Kim, Seung-Jin Lee, Wook-Joon Yu, Jong-Min Kim","doi":"10.12717/DR.2022.26.3.99","DOIUrl":"https://doi.org/10.12717/DR.2022.26.3.99","url":null,"abstract":"Abstract Styrene is the precursor of polystyrene. Human exposure to styrene could occur in occupational and residential settings and via food intake. Styrene is metabolized to styrene-7,8-oxide by cytochrome P450 enzyme. In the present study, we investigated the cytotoxicity mediated by styrene and styrene-7,8-oxide in TM3 testicular Leydig cells in vitro. We first monitored the nuclear fragmentation in Leydig cells after exposure to styrene or styrene-7,8-oxide. Hoechst 33258 cell staining showed that styrene exposure in TM3 Leydig cells did not exhibit nuclear fragmentation at any concentration. In contrast, nuclear fragmentation was seen in styrene-7,8-oxide-exposed cells. These results indicate that cytotoxicity-mediated cell death in Leydig cells is more susceptible to styrene-7,8-oxide than to styrene. Following styrene treatment, procaspase-3 and XIAP protein levels did not show significant changes, and cleaved (active) forms of caspase-3 were not detected. Consistent with the western blot results, the active forms of caspase-3 and XIAP proteins were not prominently altered in the cytoplasm of cells treated with styrene. In contrast to styrene, styrene-7,8-oxide induced cell death in an apoptotic fashion, as seen in caspase-3 activation and increased the expression of XIAP proteins. Taken together, the results obtained in this study demonstrate a fundamental idea that Leydig cells are capable of protecting themselves from cytotoxicity-mediated apoptosis as a result of styrene exposure in vitro. It remains unclear whether the steroid-producing function, i.e., steroidogenesis, of Leydig cells is also unaffected by exposure to styrene. Therefore, further studies are needed to elucidate the endocrine disrupting potential of styrene in Leydig cells.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"26 1","pages":"99 - 105"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44983696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Drp1 Expression and Phosphorylation in Steroidogenic Corpus Luteum during the Estrous Cycle in Rat Ovaries. 大鼠卵巢发情周期中甾体黄体中Drp1的表达和磷酸化。

Development & reproduction Pub Date : 2022-06-01 Epub Date: 2022-06-30 DOI: 10.12717/DR.2022.26.2.71

Ji-Eun Park, Seung Gee Lee, Young Hyun Yoo, Jong-Min Kim

{"title":"Drp1 Expression and Phosphorylation in Steroidogenic Corpus Luteum during the Estrous Cycle in Rat Ovaries.","authors":"Ji-Eun Park, Seung Gee Lee, Young Hyun Yoo, Jong-Min Kim","doi":"10.12717/DR.2022.26.2.71","DOIUrl":"https://doi.org/10.12717/DR.2022.26.2.71","url":null,"abstract":"In response to luteinizing hormone (LH), a higher concentration of progesterone (P4) is produced in luteal cells of corpus luteum (CL). Mitochondria are an essential cellular organelle in steroidogenesis. The specific engagement of the concept regarding mitochondrial shaping with early stages of steroidogenesis was suggested in reproductive endocrine cells. Although the specific involvement of GTPase dynamin-related protein 1 (Drp1) with steroidogenesis has been demonstrated in luteal cells of bovine CL in vitro, its actual relationship with ovarian steroidogenesis during the estrous cycle remains unknown. In this study, while Fis1 and Opa1 protein levels did not show significant changes during the estrous cycle, Drp1, Mfn1, and Mfn2 proteins exhibited relatively lower levels at proestrus than at estrus or diestrus. 3β-HSD showed higher levels at proestrus than at estrus or diestrus. In addition, Drp1 phosphorylation (s637) was higher in proestrus than in estrus or diestrus. Immune-positive cells for Drp1, pDrp1 (s637), and 3β-HSD were all localized in the cytoplasm of luteal cells in the CL. The immune-positive cells for 3β-HSD were more frequently seen in the CL at proestrus than at estrus or diestrus. Immunoreactivity for Drp1 in luteal cells at proestrus was weaker than that at estrus or diestrus. However, pDrp1 (s637) immune-positive cells were mostly detected in luteal cells at proestrus. These results imply that steroidogenesis (P4 production) in the CL is closely related to phosphorylation of Drp1 at serine 637. Taken together, this study presents evidence that Drp1 phosphorylation at serine 637 is an important step in steroidogenesis in the CL.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"26 2","pages":"71-77"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a5/4d/dr-26-2-71.PMC9336213.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40709075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

PCR Analysis for Genetic Distances of Two Charybdis Crab Populations. 两个沙蟹居群遗传距离的PCR分析。

Development & reproduction Pub Date : 2022-06-01 Epub Date: 2022-06-30 DOI: 10.12717/DR.2022.26.2.91

Jong-Man Yoon

{"title":"PCR Analysis for Genetic Distances of Two Charybdis Crab Populations.","authors":"Jong-Man Yoon","doi":"10.12717/DR.2022.26.2.91","DOIUrl":"https://doi.org/10.12717/DR.2022.26.2.91","url":null,"abstract":"Genomic DNA (gDNA) set apart from two populations of Korean Charybdis crab (Charybdis japonica) was augmented by PCR experiments. The five oligonucleotides primers (ONT-primers) were spent to yield the number of unique loci shared to each crab population (ULSECP) and number of loci shared by the two crab populations (LSTCP). 305 fragments (FRAGs) were identified in the Charybdis crab population A (CCPA), and 344 in the Charybdis crab population B (CCPB): 44 number of ULSECP (14.43%) in the CCPA and 110 (31.98%) in the CCPB. 44 number of LSTCP, with an average of 8.8 per primer, were detected in the two crab populations. The bandsharing (BS) value between entity's no. 01 and no. 10 was the lowest (0.371) between the two CCPs. The average bandsharing (ABS) values of individuals in the CCPA (0.575±0.014) were lesser than in those originated from the CCPB (0.705±0.011) (p < 0.05). The polar hierarchical dendrogram (PHD) achieved by the five ONT-primers denotes three genetic clusters (GCs): cluster I (CHARYBCRAB 01, 04, 05, 06, and 08), cluster II (CHARYBCRAB 02, 03, 07, 09, 10, and 11) and cluster III (CHARYBCRAB 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, and 22). The shortest genetic distance (GD) displaying significant molecular difference (MD) was between individuals CHARYBCRAB no. 18 and CHARYBCRAB no. 17 (0.055).","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"26 2","pages":"91-98"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b4/11/dr-26-2-91.PMC9336212.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40599997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Zebrafish Klf11b is Required to Maintain Cell Viability by Inhibiting p53-Mediated Apoptosis. 斑马鱼需要Klf11b通过抑制p53介导的凋亡来维持细胞活力。

Development & reproduction Pub Date : 2022-06-01 Epub Date: 2022-06-30 DOI: 10.12717/DR.2022.26.2.79

Hee Jeong Kong, Jung Jin Lee, Ju-Won Kim, Julan Kim, Young-Ok Kim, Sang-Yeob Yeo

引用次数: 1