Development & reproduction最新文献_第5页

Kidney Organoid Derived from Human Pluripotent and Adult Stem Cells for Disease Modeling. 从人类多能干细胞和成体干细胞衍生的肾脏类器官用于疾病建模。

Development & reproduction Pub Date : 2023-06-01 DOI: 10.12717/DR.2023.27.2.57

Hyun Mi Kang

引用次数: 0

Gene Expression Profiles of Long-Chain Acyl-Coenzyme A Dehydrogenase, Nuclear Distribution C-Containing Protein 3, and Receptor Tyrosine Kinase Tie-1 in Swimming Larva of Sea Cucumber Apostichopus japonicus. 长链酰基辅酶A脱氢酶、核分布含c蛋白3和受体酪氨酸激酶Tie-1在刺参游动仔鱼中的基因表达谱

Development & reproduction Pub Date : 2023-06-01 DOI: 10.12717/DR.2023.27.2.91

Sehwan Kim, Seungheon Lee, Gil Jung Kim, Young Chang Sohn

{"title":"Gene Expression Profiles of Long-Chain Acyl-Coenzyme A Dehydrogenase, Nuclear Distribution C-Containing Protein 3, and Receptor Tyrosine Kinase Tie-1 in Swimming Larva of Sea Cucumber Apostichopus japonicus.","authors":"Sehwan Kim, Seungheon Lee, Gil Jung Kim, Young Chang Sohn","doi":"10.12717/DR.2023.27.2.91","DOIUrl":"https://doi.org/10.12717/DR.2023.27.2.91","url":null,"abstract":"The sea cucumber, Apostichopus japonicus, is one of the most valuable aquatic species. The color of body wall and appearance are important for the value of sea cucumbers. To examine expression pattern of long-chain acyl-coenzyme A dehydrogenase (LCAD), nuclear distribution C-containing protein 3 (NUDCD3), and receptor tyrosine kinase Tie-1 (TIE1), previously reported as differently expressed genes during the pigmentation of sea cucumber, we analyzed the temporal profiles of LCAD, NUDCD3, and TIE1 mRNAs in LED-exposed and light-shielded A. japonicus. Real-time quantitative PCR revealed that the LCAD, NUDCD3, and TIE1 mRNAs from the juveniles at 40-60 days post-fertilization (dpf) exhibited increasing patterns as compared to those of an early developmental larva (6-dpf). At 60-dpf juveniles, the LCAD and TIE1 mRNA levels of LED-exposed individuals were higher than those of light-shielded ones, whereas at 40-dpf and 50-dpf juveniles, the NUDCD3 mRNA expression was higher in the light-shielded condition (p<0.05). In the pigmented juveniles (90-dpf), the LCAD and TIE1 mRNA levels tended to show higher levels in red individuals than those in green ones, but there was a conversely higher level of NUDCD3 mRNA in green larva. In situ examination of LCAD and NUDCD3 mRNAs in light-shielded 6-dpf larva revealed that both genes are mainly expressed in the internal organs compared to the body surface. Together, these results may provide insights into the differential gene expression of LCAD, NUDCD3, and TIE1 during pigmentation process of the sea cucumber.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"27 2","pages":"91-99"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/85/ac/dr-27-2-91.PMC10390100.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9917085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Acetaminophen on Reproductive Activities in Male Golden Hamsters 对乙酰氨基酚对雄性金仓鼠生殖活动的影响

Development & reproduction Pub Date : 2023-03-31 DOI: 10.12717/dr.2023.27.1.5

Chae Yeon Lee, Hyeonji Hwang, Jin-Soo Park, Sung-Ho Lee, Chang Eun Park, Yong-Pil Cheon, Donchan Choi

{"title":"Effects of Acetaminophen on Reproductive Activities in Male Golden Hamsters","authors":"Chae Yeon Lee, Hyeonji Hwang, Jin-Soo Park, Sung-Ho Lee, Chang Eun Park, Yong-Pil Cheon, Donchan Choi","doi":"10.12717/dr.2023.27.1.5","DOIUrl":"https://doi.org/10.12717/dr.2023.27.1.5","url":null,"abstract":"Acetaminophen [Paracetamol, N-acetyl-para-aminophenol (APAP)] is a common over-the- counter analgesic agent as nonsteroidal anti-inflammatory drugs (NSAIDs). The high doses or the long-term treatment of acetaminophen via usual gavage feeding resulted in damage of testicles that presented recoverable impairment, as well as liver and kidney. The influence of acetaminophen was examined in male golden hamsters treated with acetaminophen- containing diet feeding. They were divided into 5 groups and subjected to this experiment for 4 weeks: animals housed in long photoperiod (LP) as LP control, animals housed in short photoperiod (SP) for 4 weeks as SP control (SP4), and groups of animals treated with low, middle, and high concentrations of acetaminophen (Low, Middle, High groups). Also animals housed in SP for 8 weeks were included (SP8) to contrast testicular activities, if necessary. As results, spermatozoa filled the seminiferous tubules of the testicles of animals in LP control and SP4 groups. The aspects were seen in the animals taken diets of low and middle doses of acetaminophen. The animals who fed high dose of acetaminophen showed large or small testicles. The large testicles displayed all germ cells at the steps of spermatogenesis. The small testicles presented no sperm as the animals housed in SP for 8 weeks. Thus these results indicate that acetaminophen invokes the antigonadal effects and accelerates the regressing process of the testicles in the animals compared to the animals exposed to SP.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135897784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid Polysaccharides have a Detrimental Effect on the Function of the Ovaries and Uterus in Mice through Increased Pro-Inflammatory Cytokines. 脂质多糖通过增加促炎性细胞因子对小鼠卵巢和子宫功能产生不利影响。

Development & reproduction Pub Date : 2022-12-01 DOI: 10.12717/DR.2022.26.4.135

Jihyeon Seo, Jungmin Lee, Sua Kim, Minji Lee, Hyunwon Yang

{"title":"Lipid Polysaccharides have a Detrimental Effect on the Function of the Ovaries and Uterus in Mice through Increased Pro-Inflammatory Cytokines.","authors":"Jihyeon Seo, Jungmin Lee, Sua Kim, Minji Lee, Hyunwon Yang","doi":"10.12717/DR.2022.26.4.135","DOIUrl":"https://doi.org/10.12717/DR.2022.26.4.135","url":null,"abstract":"As the number of coronavirus disease 2019 (COVID-19) vaccinations increases, various side effects are being reported, and menstrual abnormalities have been reported as a side effect in women. However, it is still unclear whether the COVID-19 vaccine has detrimental effects on the female reproductive system. Therefore, we investigated the effect of excessive immune response on reproductive function by administering Lipopolysaccharides (LPS) instead of the COVID-19 vaccine. The immune response in mice was induced by injection of LPS. Mice injected with saline 5 times were used as a control group, and mice injected with LPS 5 times were used as an experimental group. Repeated administration of LPS significantly reduced the number of corpus luteum (CL). On the other hand, the injection of LPS did not affect the development of follicles leading before the CL. The expression of the apoptosis-related genes Fas and Fas-L increased in the experimental group. In addition, the expression of the inflammation-related genes increased in the experimental group. In this study, we confirmed that LPS had detrimental effects on the uterus and ovaries in mice. These results suggest that injection of LPS can cause immune reactions within the uterus and ovaries and cause hormonal changes, which can have adverse effects such as abnormal operation or bleeding of the menstrual cycle. These results are expected to help determine the cause of decreased reproductive function, infertility, or physiological disorders caused by the COVID-19 vaccine.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"26 4","pages":"135-144"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6c/cf/dr-26-4-135.PMC9925187.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9315417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of Nonylphenol on the Structure of Adrenal Cortex in F1 Generation Rats. 壬基酚对F1代大鼠肾上腺皮质结构的影响。

Development & reproduction Pub Date : 2022-12-01 DOI: 10.12717/DR.2022.26.4.175

Hee-Su Kim, Sung-Ho Lee

{"title":"Effect of Nonylphenol on the Structure of Adrenal Cortex in F1 Generation Rats.","authors":"Hee-Su Kim, Sung-Ho Lee","doi":"10.12717/DR.2022.26.4.175","DOIUrl":"https://doi.org/10.12717/DR.2022.26.4.175","url":null,"abstract":"Previous studies, including our own, indicate that distinct morphological changes in rodent adrenal cortex could be induced by exposure of endocrine disrupting chemicals (EDC). In the present study, we conducted histological analyses of adrenocortical substructure using a nonylphenol-treated F1 rat model. The adrenal weight of NP-5000 group was significantly declined in female rats (p<0.001), while the adrenal weights of NP-treated groups were not significantly changed in male rats. The thickness of zona glomerulosa layers of female rats in NP-5000 group was significantly declined (p<0.001) but zona fasciculata layers were not changed. The zona reticularis layers of NP-treated group were significantly thinner than those of control group (NP-50, p<0.05; NP-5000, p<0.01). In male adrenal glands, there was no significant change of zona glomerulosa layers in NP-treated groups while the thickness of zona fasciculata in NP-5000 group was significantly decreased (p<0.01). Like female rats, the thickness of zona reticularis in NP-treated groups was significantly decreased (NP-50, p<0.001; NP-5000, p<0.05). Present study demonstrated that the adrenal histology could be altered by low-dose NP exposure in F1 rats, and the effect might be sexually dimorphic. Further study will be helpful for understanding possible adrenal pathophysiology induced by EDC exposure, and EDC-related sexually dimorphic phenomena in rodent adrenals.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"26 4","pages":"175-182"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a8/68/dr-26-4-175.PMC9925185.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Antibiotics on the Uterine Microbial Community of Mice. 抗生素对小鼠子宫微生物群落的影响。

Development & reproduction Pub Date : 2022-12-01 DOI: 10.12717/DR.2022.26.4.145

Sang-Gyu Kim, Dae-Wi Kim, Hoon Jang

{"title":"Effects of Antibiotics on the Uterine Microbial Community of Mice.","authors":"Sang-Gyu Kim, Dae-Wi Kim, Hoon Jang","doi":"10.12717/DR.2022.26.4.145","DOIUrl":"https://doi.org/10.12717/DR.2022.26.4.145","url":null,"abstract":"The gut microbiota is involved in the maintenance of physiological homeostasis and is now recognized as a regulator of many diseases. Although germ-free mouse models are the standard for microbiome studies, mice with antibiotic-induced sterile intestines are often chosen as a fast and inexpensive alternative. Pathophysiological changes in the gut microbiome have been demonstrated, but there are no reports so far on how such alterations affect the bacterial composition of the uterus. Here we examined changes in uterine microbiota as a result of gut microbiome disruption in an antibiotics-based sterile-uterus mouse model. Sterility was induced in 6-week-old female mice by administration of a combination of antibiotics, and amplicons of a bacteria marker gene (16S rRNA) were sequenced to decipher bacterial community structures in the uterus. At the phylum-level, Proteobacteria, Firmicutes, and Actinobacteria were found to be dominant, while Ralstonia, Escherichia, and Prauserella were the major genera. Quantitative comparisons of the microbial contents of an antibiotic-fed and a control group revealed that the treatment resulted in the reduction of bacterial population density. Although there was no significant difference in bacterial community structures between the two animal groups, β-diversity analysis showed a converged profile of uterus microbiotain the germ-free model. These findings suggest that the induction of sterility does not result in changes in the levels of specific taxa but in a reduction of individual variations in the mouse uterus microbiota, accompanied by a decrease in overall bacterial population density.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"26 4","pages":"145-153"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/99/10/dr-26-4-145.PMC9925184.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between Condition Index Values and Expression Levels of Gene and Protein in the Adductor Muscle of Diploid and Triploid Oysters Crassostrea gigas. 二倍体和三倍体牡蛎内收肌基因和蛋白表达量与状态指数值的关系。

Development & reproduction Pub Date : 2022-12-01 DOI: 10.12717/DR.2022.26.4.165

Su-Jin Park, Youn Hee Choi

{"title":"Relationship between Condition Index Values and Expression Levels of Gene and Protein in the Adductor Muscle of Diploid and Triploid Oysters Crassostrea gigas.","authors":"Su-Jin Park, Youn Hee Choi","doi":"10.12717/DR.2022.26.4.165","DOIUrl":"https://doi.org/10.12717/DR.2022.26.4.165","url":null,"abstract":"Three proteins [myosin heavy chain (MHC), filamin-C fragment (FIL-C), and actin 2 (ACT2)] were identified in adductor muscle from diploid and triploid Pacific oysters (Crassostrea gigas) and the relationship between the condition index (CI) and mRNA expression of these genes was investigated, together with the mRNA expression of molluscan insulin-related peptide (MIP), C. gigas insulin receptor-related receptor (CIR), and insulin-like growth factor binding protein complex acid labile subunit (IGFBP-ALS). Monthly changes in the CI were similar to the changes in the tissue weight rate in both groups. ACT2 and MHC mRNA expression was statistically higher in the triploid than the diploid, while FIL-C mRNA expression was significantly higher in the diploid (p<0.05). The MIP, CIR, and IGFBP-ALS mRNA expression of the diploid oysters were all significantly higher in July than in other months (p<0.05). The MIP, CIR, and IGFBP-ALS mRNA expression in the triploid oysters was high in July, but there were no significant differences (p>0.05). Changes in the expression levels of the genes investigated in this study could be used as intrinsic indicators of the annual growth, maturity, and spawning period of cultured diploid and triploid C. gigas in Tongyeong, Korea.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"26 4","pages":"165-174"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b9/98/dr-26-4-165.PMC9925188.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Pluripotent Stem Cell-Derived Alveolar Epithelial Cells as a Tool to Assess Cytotoxicity of Particulate Matter and Cigarette Smoke Extract. 人类多能干细胞衍生的肺泡上皮细胞作为评估颗粒物质和香烟烟雾提取物细胞毒性的工具。

Development & reproduction Pub Date : 2022-12-01 DOI: 10.12717/DR.2022.26.4.155

Jung-Hyun Kim, Minje Kang, Ji-Hye Jung, Seung-Joon Lee, Seok-Ho Hong

{"title":"Human Pluripotent Stem Cell-Derived Alveolar Epithelial Cells as a Tool to Assess Cytotoxicity of Particulate Matter and Cigarette Smoke Extract.","authors":"Jung-Hyun Kim, Minje Kang, Ji-Hye Jung, Seung-Joon Lee, Seok-Ho Hong","doi":"10.12717/DR.2022.26.4.155","DOIUrl":"https://doi.org/10.12717/DR.2022.26.4.155","url":null,"abstract":"Human pluripotent stem cells (hPSCs) can give rise to a vast array of differentiated derivatives, which have gained great attention in the field of in vitro toxicity evaluation. We have previously demonstrated that hPSC-derived alveolar epithelial cells (AECs) are phenotypically and functionally similar to primary AECs and could be more biologically relevant alternatives for assessing the potential toxic materials including in fine dust and cigarette smoking. Therefore, in this study, we employed hPSC-AECs to evaluate their responses to exposure of various concentrations of diesel particulate matter (dPM), cigarette smoke extract (CSE) and nicotine for 48 hrs in terms of cell death, inflammation, and oxidative stress. We found that all of these toxic materials significantly upregulated the transcription of pro-inflammatory cytokines such as IL-1α, IL-β, IL-6, and TNF-α. Furthermore, the exposure of dPM (100 μg/mL) strongly induced upregulation of genes related with cell death, inflammation, and oxidative stress compared with other concentrations of CSE and nicotine. These results suggest that hPSC-AECs could be a robust in vitro platform to evaluate pulmotoxicity of various air pollutants and harmful chemicals.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"26 4","pages":"155-163"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/73/c7/dr-26-4-155.PMC9925186.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10825155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Che-Yong() Logic and Research Methodology. 智勇()逻辑和研究方法论。

Development & reproduction Pub Date : 2022-12-01 DOI: 10.12717/DR.2022.26.4.183

YongNam Yun

引用次数: 0

Expression of Kisspeptin in the Adult Hamster Testis Kisspeptin在成年仓鼠睾丸中的表达

Development & reproduction Pub Date : 2022-09-01 DOI: 10.12717/DR.2022.26.3.107

Jin-Soo Park, Y. Cheon, D. Choi, Sung-Ho Lee

{"title":"Expression of Kisspeptin in the Adult Hamster Testis","authors":"Jin-Soo Park, Y. Cheon, D. Choi, Sung-Ho Lee","doi":"10.12717/DR.2022.26.3.107","DOIUrl":"https://doi.org/10.12717/DR.2022.26.3.107","url":null,"abstract":"Abstract Kisspeptins, products of KISS1 gene, are ligands of the G-protein coupled receptor (GPR54), and the kisspeptin-GPR54 signaling has an important role as an upstream regulator of gonadotropin releasing hormone (GnRH) neurons. Interestingly, extrahypothalamic expressions of kisspeptin/GPR-54 in gonads have been found in primates and experimental rodents such as rats and mice. Hamsters, another potent experimental rodent, also have a kisspeptin-GPR54 system in their ovaries. The presence of testicular kisspeptin-GPR54 system, however, remains to be solved. The present study was undertaken to determine whether the kisspeptin is expressed in hamster testis. To do this, reverse transcription-polymerase chain reactions (RT-PCRs) and immunohistochemistry (IHC) were employed. After the nest PCR, two cDNA products (320 and 280 bp, respectively) were detected by 3% agarose gel electrophoresis, and sequencing analysis revealed that the 320 bp product was correctly amplified from hamster kisspeptin cDNA. Modest immunoreactive (IR) kisspeptins were detected in Leydig-interstitial cells, and the weak signals were detected in germ cells, mostly in round spermatids and residual bodies of elongated spermatids. In the present study, we found the kisspeptin expression in the testis of Syrian hamster. Further studies on the local role(s) of testicular kisspeptin are expected for a better understanding the physiology of hamster testis, including photoperiodic gonadal regression specifically occurred in hamster gonads.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"26 1","pages":"107 - 115"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45938089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0