Development & reproduction最新文献_第4页

Potent Influence of Exogenous Melatonin on In Vitro Oocyte Maturation in the Longchin Goby, Chaenogobius annularis 外源性褪黑素对长颏虾虎鱼体外卵母细胞成熟的影响

Development & reproduction Pub Date : 2023-09-01 DOI: 10.12717/dr.2023.27.3.127

Dae Guen Kim, In Joon Hwang, Hea Ja Baek

引用次数: 0

Hormonal Changes in Women Undergoing Bariatric Surgery: A Comparative Study with a Control Group 接受减肥手术的女性激素变化:与对照组的比较研究

Development & reproduction Pub Date : 2023-09-01 DOI: 10.12717/dr.2023.27.3.117

Taha Anbara

{"title":"Hormonal Changes in Women Undergoing Bariatric Surgery: A Comparative Study with a Control Group","authors":"Taha Anbara","doi":"10.12717/dr.2023.27.3.117","DOIUrl":"https://doi.org/10.12717/dr.2023.27.3.117","url":null,"abstract":"Bariatric surgery is an effective treatment option for obesity, but its effects on reproductive hormones are not well understood. This study aimed to investigate the impact of bariatric surgery on estrogen and progesterone levels in women with obesity. This was a prospective study that included 87 women with obesity who underwent bariatric surgery and 87 control women with obesity who did not. Blood samples were collected at baseline and at 3-month and 6-month follow-up periods to measure reproductive hormones and Adiponectin as well as medical history and physical examination for endometriosis and polycystic ovary syndrome (PCOS). The study found that sleeve gastrectomy significantly increased follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels in the bariatric surgery group compared to the control group at 3 and 6-month follow-up. There was no significant difference in the mean levels of estrogen and progesterone between the two groups at the 3-month follow-up period. Bariatric surgery leads to significant weight loss in women with obesity, but it does not seem to have a significant impact on estrogen and progesterone levels in the short-term. Future studies with longer follow-up periods are needed to investigate the long-term effects of bariatric surgery on reproductive hormones.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"92 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135735873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Polymeric Immunoglobulin Receptor Expression in Olive Flounder (Paralichthys olivaceus) against Viral Hemorrhagic Septicemia Virus. 橄榄比目鱼抗病毒性出血性败血症病毒聚合免疫球蛋白受体的表达分析。

Development & reproduction Pub Date : 2023-06-01 DOI: 10.12717/DR.2023.27.2.67

Kyung-Hee Kim, Sulhye Park, Jong-Won Park, Minhwan Jeong, Julan Kim, Hyejin Kim, Jeong-Ho Lee, Dain Lee

引用次数: 0

Metformin Induces Lipogenesis and Apoptosis in H4IIE Hepatocellular Carcinoma Cells. 二甲双胍诱导 H4IIE 肝细胞癌细胞的脂肪生成和凋亡

Development & reproduction Pub Date : 2023-06-01 Epub Date: 2023-06-30 DOI: 10.12717/DR.2023.27.2.77

Deokbae Park, Sookyoung Lee, Hyejin Boo

{"title":"Metformin Induces Lipogenesis and Apoptosis in H4IIE Hepatocellular Carcinoma Cells.","authors":"Deokbae Park, Sookyoung Lee, Hyejin Boo","doi":"10.12717/DR.2023.27.2.77","DOIUrl":"10.12717/DR.2023.27.2.77","url":null,"abstract":"Metformin is the most widely used anti-diabetic drug that helps maintain normal blood glucose levels primarily by suppressing hepatic gluconeogenesis in type II diabetic patients. We previously found that metformin induces apoptotic death in H4IIE rat hepatocellular carcinoma cells. Despite its anti-diabetic roles, the effect of metformin on hepatic de novo lipogenesis (DNL) remains unclear. We investigated the effect of metformin on hepatic DNL and apoptotic cell death in H4IIE cells. Metformin treatment stimulated glucose consumption, lactate production, intracellular fat accumulation, and the expressions of lipogenic proteins. It also stimulated apoptosis but reduced autophagic responses. These metformin-induced changes were clearly reversed by compound C, an inhibitor of AMP-activated protein kinase (AMPK). Interestingly, metformin massively increased the production of reactive oxygen species (ROS), which was completely blocked by compound C. Metformin also stimulated the phosphorylation of p38 mitogen-activated protein kinase (p38MAPK). Finally, inhibition of p38MAPK mimicked the effects of compound C, and suppressed the metformin-induced fat accumulation and apoptosis. Taken together, metformin stimulates dysregulated glucose metabolism, intracellular fat accumulation, and apoptosis. Our findings suggest that metformin induces excessive glucose-induced DNL, oxidative stress by ROS generation, activation of AMPK and p38MAPK, suppression of autophagy, and ultimately apoptosis.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"27 2","pages":"77-89"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/57/77/dr-27-2-77.PMC10390098.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9922860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kidney Organoid Derived from Human Pluripotent and Adult Stem Cells for Disease Modeling. 从人类多能干细胞和成体干细胞衍生的肾脏类器官用于疾病建模。

Development & reproduction Pub Date : 2023-06-01 DOI: 10.12717/DR.2023.27.2.57

Hyun Mi Kang

引用次数: 0

Gene Expression Profiles of Long-Chain Acyl-Coenzyme A Dehydrogenase, Nuclear Distribution C-Containing Protein 3, and Receptor Tyrosine Kinase Tie-1 in Swimming Larva of Sea Cucumber Apostichopus japonicus. 长链酰基辅酶A脱氢酶、核分布含c蛋白3和受体酪氨酸激酶Tie-1在刺参游动仔鱼中的基因表达谱

Development & reproduction Pub Date : 2023-06-01 DOI: 10.12717/DR.2023.27.2.91

Sehwan Kim, Seungheon Lee, Gil Jung Kim, Young Chang Sohn

{"title":"Gene Expression Profiles of Long-Chain Acyl-Coenzyme A Dehydrogenase, Nuclear Distribution C-Containing Protein 3, and Receptor Tyrosine Kinase Tie-1 in Swimming Larva of Sea Cucumber Apostichopus japonicus.","authors":"Sehwan Kim, Seungheon Lee, Gil Jung Kim, Young Chang Sohn","doi":"10.12717/DR.2023.27.2.91","DOIUrl":"https://doi.org/10.12717/DR.2023.27.2.91","url":null,"abstract":"The sea cucumber, Apostichopus japonicus, is one of the most valuable aquatic species. The color of body wall and appearance are important for the value of sea cucumbers. To examine expression pattern of long-chain acyl-coenzyme A dehydrogenase (LCAD), nuclear distribution C-containing protein 3 (NUDCD3), and receptor tyrosine kinase Tie-1 (TIE1), previously reported as differently expressed genes during the pigmentation of sea cucumber, we analyzed the temporal profiles of LCAD, NUDCD3, and TIE1 mRNAs in LED-exposed and light-shielded A. japonicus. Real-time quantitative PCR revealed that the LCAD, NUDCD3, and TIE1 mRNAs from the juveniles at 40-60 days post-fertilization (dpf) exhibited increasing patterns as compared to those of an early developmental larva (6-dpf). At 60-dpf juveniles, the LCAD and TIE1 mRNA levels of LED-exposed individuals were higher than those of light-shielded ones, whereas at 40-dpf and 50-dpf juveniles, the NUDCD3 mRNA expression was higher in the light-shielded condition (p<0.05). In the pigmented juveniles (90-dpf), the LCAD and TIE1 mRNA levels tended to show higher levels in red individuals than those in green ones, but there was a conversely higher level of NUDCD3 mRNA in green larva. In situ examination of LCAD and NUDCD3 mRNAs in light-shielded 6-dpf larva revealed that both genes are mainly expressed in the internal organs compared to the body surface. Together, these results may provide insights into the differential gene expression of LCAD, NUDCD3, and TIE1 during pigmentation process of the sea cucumber.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"27 2","pages":"91-99"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/85/ac/dr-27-2-91.PMC10390100.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9917085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Acetaminophen on Reproductive Activities in Male Golden Hamsters 对乙酰氨基酚对雄性金仓鼠生殖活动的影响

Development & reproduction Pub Date : 2023-03-31 DOI: 10.12717/dr.2023.27.1.5

Chae Yeon Lee, Hyeonji Hwang, Jin-Soo Park, Sung-Ho Lee, Chang Eun Park, Yong-Pil Cheon, Donchan Choi

{"title":"Effects of Acetaminophen on Reproductive Activities in Male Golden Hamsters","authors":"Chae Yeon Lee, Hyeonji Hwang, Jin-Soo Park, Sung-Ho Lee, Chang Eun Park, Yong-Pil Cheon, Donchan Choi","doi":"10.12717/dr.2023.27.1.5","DOIUrl":"https://doi.org/10.12717/dr.2023.27.1.5","url":null,"abstract":"Acetaminophen [Paracetamol, N-acetyl-para-aminophenol (APAP)] is a common over-the- counter analgesic agent as nonsteroidal anti-inflammatory drugs (NSAIDs). The high doses or the long-term treatment of acetaminophen via usual gavage feeding resulted in damage of testicles that presented recoverable impairment, as well as liver and kidney. The influence of acetaminophen was examined in male golden hamsters treated with acetaminophen- containing diet feeding. They were divided into 5 groups and subjected to this experiment for 4 weeks: animals housed in long photoperiod (LP) as LP control, animals housed in short photoperiod (SP) for 4 weeks as SP control (SP4), and groups of animals treated with low, middle, and high concentrations of acetaminophen (Low, Middle, High groups). Also animals housed in SP for 8 weeks were included (SP8) to contrast testicular activities, if necessary. As results, spermatozoa filled the seminiferous tubules of the testicles of animals in LP control and SP4 groups. The aspects were seen in the animals taken diets of low and middle doses of acetaminophen. The animals who fed high dose of acetaminophen showed large or small testicles. The large testicles displayed all germ cells at the steps of spermatogenesis. The small testicles presented no sperm as the animals housed in SP for 8 weeks. Thus these results indicate that acetaminophen invokes the antigonadal effects and accelerates the regressing process of the testicles in the animals compared to the animals exposed to SP.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135897784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid Polysaccharides have a Detrimental Effect on the Function of the Ovaries and Uterus in Mice through Increased Pro-Inflammatory Cytokines. 脂质多糖通过增加促炎性细胞因子对小鼠卵巢和子宫功能产生不利影响。

Development & reproduction Pub Date : 2022-12-01 DOI: 10.12717/DR.2022.26.4.135

Jihyeon Seo, Jungmin Lee, Sua Kim, Minji Lee, Hyunwon Yang

{"title":"Lipid Polysaccharides have a Detrimental Effect on the Function of the Ovaries and Uterus in Mice through Increased Pro-Inflammatory Cytokines.","authors":"Jihyeon Seo, Jungmin Lee, Sua Kim, Minji Lee, Hyunwon Yang","doi":"10.12717/DR.2022.26.4.135","DOIUrl":"https://doi.org/10.12717/DR.2022.26.4.135","url":null,"abstract":"As the number of coronavirus disease 2019 (COVID-19) vaccinations increases, various side effects are being reported, and menstrual abnormalities have been reported as a side effect in women. However, it is still unclear whether the COVID-19 vaccine has detrimental effects on the female reproductive system. Therefore, we investigated the effect of excessive immune response on reproductive function by administering Lipopolysaccharides (LPS) instead of the COVID-19 vaccine. The immune response in mice was induced by injection of LPS. Mice injected with saline 5 times were used as a control group, and mice injected with LPS 5 times were used as an experimental group. Repeated administration of LPS significantly reduced the number of corpus luteum (CL). On the other hand, the injection of LPS did not affect the development of follicles leading before the CL. The expression of the apoptosis-related genes Fas and Fas-L increased in the experimental group. In addition, the expression of the inflammation-related genes increased in the experimental group. In this study, we confirmed that LPS had detrimental effects on the uterus and ovaries in mice. These results suggest that injection of LPS can cause immune reactions within the uterus and ovaries and cause hormonal changes, which can have adverse effects such as abnormal operation or bleeding of the menstrual cycle. These results are expected to help determine the cause of decreased reproductive function, infertility, or physiological disorders caused by the COVID-19 vaccine.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"26 4","pages":"135-144"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6c/cf/dr-26-4-135.PMC9925187.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9315417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of Nonylphenol on the Structure of Adrenal Cortex in F1 Generation Rats. 壬基酚对F1代大鼠肾上腺皮质结构的影响。

Development & reproduction Pub Date : 2022-12-01 DOI: 10.12717/DR.2022.26.4.175

Hee-Su Kim, Sung-Ho Lee

{"title":"Effect of Nonylphenol on the Structure of Adrenal Cortex in F1 Generation Rats.","authors":"Hee-Su Kim, Sung-Ho Lee","doi":"10.12717/DR.2022.26.4.175","DOIUrl":"https://doi.org/10.12717/DR.2022.26.4.175","url":null,"abstract":"Previous studies, including our own, indicate that distinct morphological changes in rodent adrenal cortex could be induced by exposure of endocrine disrupting chemicals (EDC). In the present study, we conducted histological analyses of adrenocortical substructure using a nonylphenol-treated F1 rat model. The adrenal weight of NP-5000 group was significantly declined in female rats (p<0.001), while the adrenal weights of NP-treated groups were not significantly changed in male rats. The thickness of zona glomerulosa layers of female rats in NP-5000 group was significantly declined (p<0.001) but zona fasciculata layers were not changed. The zona reticularis layers of NP-treated group were significantly thinner than those of control group (NP-50, p<0.05; NP-5000, p<0.01). In male adrenal glands, there was no significant change of zona glomerulosa layers in NP-treated groups while the thickness of zona fasciculata in NP-5000 group was significantly decreased (p<0.01). Like female rats, the thickness of zona reticularis in NP-treated groups was significantly decreased (NP-50, p<0.001; NP-5000, p<0.05). Present study demonstrated that the adrenal histology could be altered by low-dose NP exposure in F1 rats, and the effect might be sexually dimorphic. Further study will be helpful for understanding possible adrenal pathophysiology induced by EDC exposure, and EDC-related sexually dimorphic phenomena in rodent adrenals.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"26 4","pages":"175-182"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a8/68/dr-26-4-175.PMC9925185.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Antibiotics on the Uterine Microbial Community of Mice. 抗生素对小鼠子宫微生物群落的影响。

Development & reproduction Pub Date : 2022-12-01 DOI: 10.12717/DR.2022.26.4.145

Sang-Gyu Kim, Dae-Wi Kim, Hoon Jang

{"title":"Effects of Antibiotics on the Uterine Microbial Community of Mice.","authors":"Sang-Gyu Kim, Dae-Wi Kim, Hoon Jang","doi":"10.12717/DR.2022.26.4.145","DOIUrl":"https://doi.org/10.12717/DR.2022.26.4.145","url":null,"abstract":"The gut microbiota is involved in the maintenance of physiological homeostasis and is now recognized as a regulator of many diseases. Although germ-free mouse models are the standard for microbiome studies, mice with antibiotic-induced sterile intestines are often chosen as a fast and inexpensive alternative. Pathophysiological changes in the gut microbiome have been demonstrated, but there are no reports so far on how such alterations affect the bacterial composition of the uterus. Here we examined changes in uterine microbiota as a result of gut microbiome disruption in an antibiotics-based sterile-uterus mouse model. Sterility was induced in 6-week-old female mice by administration of a combination of antibiotics, and amplicons of a bacteria marker gene (16S rRNA) were sequenced to decipher bacterial community structures in the uterus. At the phylum-level, Proteobacteria, Firmicutes, and Actinobacteria were found to be dominant, while Ralstonia, Escherichia, and Prauserella were the major genera. Quantitative comparisons of the microbial contents of an antibiotic-fed and a control group revealed that the treatment resulted in the reduction of bacterial population density. Although there was no significant difference in bacterial community structures between the two animal groups, β-diversity analysis showed a converged profile of uterus microbiotain the germ-free model. These findings suggest that the induction of sterility does not result in changes in the levels of specific taxa but in a reduction of individual variations in the mouse uterus microbiota, accompanied by a decrease in overall bacterial population density.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"26 4","pages":"145-153"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/99/10/dr-26-4-145.PMC9925184.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0