Development & reproduction最新文献_第4页

Lin28 is Required for Single Niche Development in the Drosophila Male Gonad. Lin28是果蝇雄性性腺单龛发育的必要条件

Development & reproduction Pub Date : 2023-12-01 Epub Date: 2023-12-31 DOI: 10.12717/DR.2023.27.4.221

Perinthottathil Sreejith, Changsoo Kim

引用次数: 0

Tumor Immune Microenvironment as a New Therapeutic Target for Hepatocellular Carcinoma Development. 将肿瘤免疫微环境作为肝细胞癌发展的新治疗靶点

Development & reproduction Pub Date : 2023-12-01 Epub Date: 2023-12-31 DOI: 10.12717/DR.2023.27.4.167

Eunjeong Kim

引用次数: 0

Is Autophagy a Prerequisite for Steroidogenesis in Leydig Cells? 间质细胞自噬是甾体形成的先决条件吗?

Development & reproduction Pub Date : 2023-09-01 DOI: 10.12717/dr.2023.27.3.149

Ji-Eun Park, Yoon-Jae Kim, Jong-Min Kim

{"title":"Is Autophagy a Prerequisite for Steroidogenesis in Leydig Cells?","authors":"Ji-Eun Park, Yoon-Jae Kim, Jong-Min Kim","doi":"10.12717/dr.2023.27.3.149","DOIUrl":"https://doi.org/10.12717/dr.2023.27.3.149","url":null,"abstract":"We investigated the involvement of autophagy with steroidogenesis in testicular Leydig cells. Human chorionic gonadotropin (hCG)-stimulated T production in Leydig cells was not remarkably altered in the presence of an autophagy inhibitor 3-methyladenine (3-MA). Although pretreatment with 3-MA demonstrated a tendency to decrease hCG-induced T production, the differences were significant only at a higher time point of 24 h following hCG. Microtubule associated protein light chain 3 (LC3)-II was detectable in the control cells in all the experiments. The hCG-induced increase in steroidogenic acute regulatory protein (StAR) and cytochrome P450 side chain cleave (P450scc) protein levels were not significantly altered by 3-MA. Leydig cells isolated from immature rat testes 12 h following hCG treatment showed relatively increased levels of LC3-II protein compared to the control group. Furthermore, LC3-II levels shown in these cells reached almost the identical to those from normal adult testes. However, LC3-II protein levels were almost comparable or even slightly lower than the controls at 48 h following hCG. Expression of StAR and P450scc was upregulated at both 12 and 48 h after hCG. We also used MA-10 cells, the mouse Leydig cell line, in this experiment. When dibutyryl cyclic-AMP was treated with MA-10 cells, P4 levels were significantly increased in the cell culture medium. However, P4 levels tended to decrease in the presence of 3-MA, but the difference was not statistically significant. This was consistent with the results of the rat Leydig cell experiments. Together, we believe that although autophagy participates in steroidogenesis and enhances steroidogenic efficacy of Leydig cells, it may not be a decisive cellular process for steroidogenesis, specifically in the mature Leydig cells.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135732593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Feasible Role of Neuropilin Signaling in Pharyngeal Pouch Formation in Zebrafish 神经肽信号在斑马鱼咽袋形成中的可能作用

Development & reproduction Pub Date : 2023-09-01 DOI: 10.12717/dr.2023.27.3.137

Chong Pyo Choe

{"title":"A Feasible Role of Neuropilin Signaling in Pharyngeal Pouch Formation in Zebrafish","authors":"Chong Pyo Choe","doi":"10.12717/dr.2023.27.3.137","DOIUrl":"https://doi.org/10.12717/dr.2023.27.3.137","url":null,"abstract":"Pharyngeal pouches are an important epithelial structure controlling facial skeletal development in vertebrates. A series of pouches arise sequentially in the pharyngeal endoderm through collective cell migration followed by rearrangement of pouch-forming cells. While crucial transcription factors and signaling molecules have been identified in pouch formation, a role for Neuropilins (Nrps) in pouch development has not yet been analyzed in any vertebrates. Nrps are cell surface receptors essential for angiogenesis and axon guidance. In all vertebrates, the two Nrp family members, Nrp1 and Nrp2, are conserved in the genome, with two paralogs for Nrp1 (Nrp1a and Nrp1b) and Nrp2 (Nrp2a and Nrp2b) being identified in zebrafish. Here, I report a potential requirement of Nrp signaling in pouch development in zebrafish. nrp1a and nrp2b were expressed in the developing pouches, with sema3d, a ligand for Nrps, being expressed in the pouches. Knocking down Nrps signaling in the pharyngeal endoderm led to severe defects in pouches and facial cartilages. In addition, blocking Mitogen-activated protein kinase (MAPK) activities, a downstream effector of Nrp signaling, in the pharyngeal endoderm caused similar defects in pouches and facial skeleton to those by knocking down Nrps signaling. My results suggest that Nrp signaling acts for pouch formation through MAPK.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135735863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Annual Reproductive Cycle, Proximate Composition, Fatty Acid and Amino Acid Content of Pacific Oyster, Crassostrea gigas (Magallana gigas), in Gadeok-do, Korea 韩国加德岛长牡蛎(Magallana gigas)年繁殖周期、近似组成、脂肪酸和氨基酸含量

Development & reproduction Pub Date : 2023-09-01 DOI: 10.12717/dr.2023.27.3.101

In Kyu Cho, Bong-Seung Seo, So-Yeon Hwang, Ye-In Lee, Ji-Sung Moon, Su-Jin Park, Hee-Jung Lee, Young Baek Hur, Youn Hee Choi

{"title":"The Annual Reproductive Cycle, Proximate Composition, Fatty Acid and Amino Acid Content of Pacific Oyster, Crassostrea gigas (Magallana gigas), in Gadeok-do, Korea","authors":"In Kyu Cho, Bong-Seung Seo, So-Yeon Hwang, Ye-In Lee, Ji-Sung Moon, Su-Jin Park, Hee-Jung Lee, Young Baek Hur, Youn Hee Choi","doi":"10.12717/dr.2023.27.3.101","DOIUrl":"https://doi.org/10.12717/dr.2023.27.3.101","url":null,"abstract":"Environmental factors impact oyster growth, condition, and gonadal development, which is linked to gamete characteristics observed through histology. The reproductive cycle of bivalves is related to energy storage and utilization. Therefore, in this study, the year-round growth change and gonadal development of oysters were observed using histological analysis, and the biochemical composition changes were confirmed. The oysters used in this study are being nurtured in Gadeok-do, and 40 oysters were randomly sampled monthly from March 2021 to February 2022. Result of histological analysis of gonads, oysters were showed early development from December to February, late development from March and April, mature and ripe from May to July, spawned from August to October, and spent from November to December. Condition index values of oysters decreased in summer and autumn and increased again when entered the spent after spawning. The protein content of oysters was high in May, the maturity period, and the lipid content decreased during the spawning period. In addition, EPA and DHA, the major fatty acids of oysters, were low during the spawning period and high during the maturation period. As a result, this study suggested a close relationship between changes in oyster growth, biochemical composition, and the reproductive cycle.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135735630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ruvbl1 is Essential for Ciliary Beating during Xenopus laevis Embryogenesis Ruvbl1对非洲爪蟾胚胎发生过程中纤毛跳动至关重要

Development & reproduction Pub Date : 2023-09-01 DOI: 10.12717/dr.2023.27.3.159

Chan Young Kim, Hyun-Kyung Lee, Hongchan Lee, Hyun-Shik Lee

引用次数: 0

Potent Influence of Exogenous Melatonin on In Vitro Oocyte Maturation in the Longchin Goby, Chaenogobius annularis 外源性褪黑素对长颏虾虎鱼体外卵母细胞成熟的影响

Development & reproduction Pub Date : 2023-09-01 DOI: 10.12717/dr.2023.27.3.127

Dae Guen Kim, In Joon Hwang, Hea Ja Baek

引用次数: 0

Hormonal Changes in Women Undergoing Bariatric Surgery: A Comparative Study with a Control Group 接受减肥手术的女性激素变化:与对照组的比较研究

Development & reproduction Pub Date : 2023-09-01 DOI: 10.12717/dr.2023.27.3.117

Taha Anbara

{"title":"Hormonal Changes in Women Undergoing Bariatric Surgery: A Comparative Study with a Control Group","authors":"Taha Anbara","doi":"10.12717/dr.2023.27.3.117","DOIUrl":"https://doi.org/10.12717/dr.2023.27.3.117","url":null,"abstract":"Bariatric surgery is an effective treatment option for obesity, but its effects on reproductive hormones are not well understood. This study aimed to investigate the impact of bariatric surgery on estrogen and progesterone levels in women with obesity. This was a prospective study that included 87 women with obesity who underwent bariatric surgery and 87 control women with obesity who did not. Blood samples were collected at baseline and at 3-month and 6-month follow-up periods to measure reproductive hormones and Adiponectin as well as medical history and physical examination for endometriosis and polycystic ovary syndrome (PCOS). The study found that sleeve gastrectomy significantly increased follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels in the bariatric surgery group compared to the control group at 3 and 6-month follow-up. There was no significant difference in the mean levels of estrogen and progesterone between the two groups at the 3-month follow-up period. Bariatric surgery leads to significant weight loss in women with obesity, but it does not seem to have a significant impact on estrogen and progesterone levels in the short-term. Future studies with longer follow-up periods are needed to investigate the long-term effects of bariatric surgery on reproductive hormones.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"92 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135735873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Polymeric Immunoglobulin Receptor Expression in Olive Flounder (Paralichthys olivaceus) against Viral Hemorrhagic Septicemia Virus. 橄榄比目鱼抗病毒性出血性败血症病毒聚合免疫球蛋白受体的表达分析。

Development & reproduction Pub Date : 2023-06-01 DOI: 10.12717/DR.2023.27.2.67

Kyung-Hee Kim, Sulhye Park, Jong-Won Park, Minhwan Jeong, Julan Kim, Hyejin Kim, Jeong-Ho Lee, Dain Lee

引用次数: 0

Metformin Induces Lipogenesis and Apoptosis in H4IIE Hepatocellular Carcinoma Cells. 二甲双胍诱导 H4IIE 肝细胞癌细胞的脂肪生成和凋亡

Development & reproduction Pub Date : 2023-06-01 Epub Date: 2023-06-30 DOI: 10.12717/DR.2023.27.2.77

Deokbae Park, Sookyoung Lee, Hyejin Boo

{"title":"Metformin Induces Lipogenesis and Apoptosis in H4IIE Hepatocellular Carcinoma Cells.","authors":"Deokbae Park, Sookyoung Lee, Hyejin Boo","doi":"10.12717/DR.2023.27.2.77","DOIUrl":"10.12717/DR.2023.27.2.77","url":null,"abstract":"<p><p>Metformin is the most widely used anti-diabetic drug that helps maintain normal blood glucose levels primarily by suppressing hepatic gluconeogenesis in type II diabetic patients. We previously found that metformin induces apoptotic death in H4IIE rat hepatocellular carcinoma cells. Despite its anti-diabetic roles, the effect of metformin on hepatic de novo lipogenesis (DNL) remains unclear. We investigated the effect of metformin on hepatic DNL and apoptotic cell death in H4IIE cells. Metformin treatment stimulated glucose consumption, lactate production, intracellular fat accumulation, and the expressions of lipogenic proteins. It also stimulated apoptosis but reduced autophagic responses. These metformin-induced changes were clearly reversed by compound C, an inhibitor of AMP-activated protein kinase (AMPK). Interestingly, metformin massively increased the production of reactive oxygen species (ROS), which was completely blocked by compound C. Metformin also stimulated the phosphorylation of p38 mitogen-activated protein kinase (p38MAPK). Finally, inhibition of p38MAPK mimicked the effects of compound C, and suppressed the metformin-induced fat accumulation and apoptosis. Taken together, metformin stimulates dysregulated glucose metabolism, intracellular fat accumulation, and apoptosis. Our findings suggest that metformin induces excessive glucose-induced DNL, oxidative stress by ROS generation, activation of AMPK and p38MAPK, suppression of autophagy, and ultimately apoptosis.</p>","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"27 2","pages":"77-89"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/57/77/dr-27-2-77.PMC10390098.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9922860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0