Acupuncture in Medicine最新文献

{"title":"The effectiveness and safety of acupuncture/electroacupuncture for chemotherapy-induced peripheral neuropathy: a systematic review and meta-analysis.","authors":"Li-Xia Pei,&nbsp;Yue Yi,&nbsp;Jing Guo,&nbsp;Lu Chen,&nbsp;Jin-Yong Zhou,&nbsp;Xiao-Liang Wu,&nbsp;Jian-Hua Sun,&nbsp;Hao Chen","doi":"10.1177/09645284221076512","DOIUrl":"https://doi.org/10.1177/09645284221076512","url":null,"abstract":"<p><strong>Objective: </strong>Chemotherapy-induced peripheral neuropathy (CIPN) is a dose-limiting adverse effect of anticancer agents with virtually no effective treatment. Safe and effective therapies are needed urgently. Acupuncture shows therapeutic possibilities in this regard but needs to be further evaluated.</p><p><strong>Methods: </strong>A systematic search was conducted in seven databases from their inception to April 2020. Randomized controlled trials (RCTs) focused on acupuncture/electroacupuncture (EA) for the treatment of CIPN were included. Revman 5.3 software was used for meta-analysis if there was no significant heterogeneity. Otherwise, qualitative analysis was utilized.</p><p><strong>Results: </strong>Nine studies involving 582 patients were included in this review. Most of the studies exhibited unclear risk of bias because some details were not mentioned. As the clinical heterogeneity was significant, qualitative analysis was performed to describe nerve conduction velocity, effective rate for motor neuropathy, pain scores, quality of life and adverse events. Meta-analysis was performed on four studies to analyze the effective rate for sensory neuropathy due to inconspicuous heterogeneity. The results indicated that acupuncture may generate a better effect on sensory neuropathy than vitamin B (risk ratio = 1.60, 95% confidence interval = 1.31-1.95, I² = 0%, p < 0.00001). The efficacy of EA plus glutathione (GSH) appeared to be better than that of GSH alone in alleviating sensory neurotoxicity and in improving nerve conduction velocity. Acupuncture plus methylcobalamin showed more favorable effects than methylcobalamin alone in relieving neuralgia, restoring nerve conduction velocity and improving quality of life. In terms of pain relief and improved CIPN-specific quality of life, acupuncture plus standard care was better than standard care alone. In terms of pain relief, EA was more effective than usual care.</p><p><strong>Conclusion: </strong>Acupuncture may be effective and safe in the treatment of CIPN according to the analyzed studies. However, more studies with higher methodological quality are warranted in order to be able to draw firmer conclusions. Future rigorous RCTs will be necessary to confirm the effectiveness and safety of acupuncture for CIPN.</p>","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":"41 2","pages":"73-85"},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9317230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Billing for perioperative acupuncture: current practices and future directions.","authors":"Alexander B Stone,&nbsp;Roberta J Stack,&nbsp;Stephanie I Cheng","doi":"10.1177/09645284221146203","DOIUrl":"https://doi.org/10.1177/09645284221146203","url":null,"abstract":"Perioperative acupuncture has been shown to reduce anxiety, as well as mitigate perioperative nausea.1 The addition of intra-operative acupuncture has been shown to help with post-operative pain control in total knee and anterior cruciate ligament replacements.2,3 One barrier to increasing access to perioperative acupuncture services is the lack of clear, widely applicable billing practices and guidelines. Establishing the ability to show an additional revenue stream could increase institutional support for perioperative integrative medicine programs. In this letter, we will review current billing practices for perioperative acupuncture and explore existing economic barriers to increased access for perioperative acupuncture services. Current billing practices (outpatient acupuncture)","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":"41 2","pages":"116-118"},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9324205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-oral acupuncture treatment for chronic aspiration after esophageal cancer surgery: a case report.","authors":"SunKyung Jin,&nbsp;WooJeong Lee,&nbsp;Hae Jeong Nam,&nbsp;Kyuseok Kim","doi":"10.1177/09645284221125249","DOIUrl":"https://doi.org/10.1177/09645284221125249","url":null,"abstract":"1 Department of Clinical Korean Medicine, Graduate School of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea 2Cosoom Korean Medical Clinic, Seoul, Republic of Korea 3 Department of Ophthalmology, Otorhinolaryngology and Dermatology of Korean Medicine, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea 4 Department of Ophthalmology, Otorhinolaryngology and Dermatology of Korean Medicine, Kyung Hee University College of Korean Medicine, Kyung Hee University Medical Center, Seoul, Republic of Korea","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":"41 2","pages":"110-111"},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9325027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Manual acupuncture at ST36 attenuates rheumatoid arthritis by inhibiting M1 macrophage polarization and enhancing Treg cell populations in adjuvant-induced arthritic rats.","authors":"Nannan Yu,&nbsp;Fuming Yang,&nbsp;Xue Zhao,&nbsp;Yongming Guo,&nbsp;Yuan Xu,&nbsp;Guangchang Pang,&nbsp;Yinan Gong,&nbsp;Shenjun Wang,&nbsp;Yangyang Liu,&nbsp;Yuxin Fang,&nbsp;Kun Yu,&nbsp;Lin Yao,&nbsp;Hui Wang,&nbsp;Kuo Zhang,&nbsp;Baohu Liu,&nbsp;Zhenguo Wang,&nbsp;Yi Guo,&nbsp;Zhifang Xu","doi":"10.1177/09645284221085278","DOIUrl":"https://doi.org/10.1177/09645284221085278","url":null,"abstract":"<p><strong>Objectives: </strong>Acupuncture has been found to be effective at relieving many inflammatory pain conditions, including rheumatoid arthritis (RA). We aimed to assess the anti-inflammatory potential of manual acupuncture (MA) treatment of RA using adjuvant-induced arthritic (AIA) rats and to explore the underlying mechanisms.</p><p><strong>Methods: </strong>The anti-inflammatory and analgesic actions of MA at ST36 (<i>Zusanli</i>) in AIA rats were assessed using paw withdrawal latency and swelling, histological examination and cytokine detection by enzyme-linked immunoassay (ELISA). The cell-cell communication (CCC) network was analyzed with a multiplex immunoassay of 24 immune factors expressed in the inflamed joints, and the macrophage and Treg populations and associated cytokines regulated by MA were investigated using reverse-transcription quantitative polymerase chain reaction (RT-qPCR), ELISA and flow cytometry.</p><p><strong>Results: </strong>MA markedly decreased heat hyperalgesia and paw swelling in AIA rats. MA-treated rats also exhibited decreased levels of pro-inflammatory cytokines (tumor necrosis factor (TNF)-α, interleukin (IL)-1β) coupled with increased anti-inflammatory cytokines (IL-10, transforming growth factor (TGF)-β1) in the ankle joints at protein and mRNA levels. CCC network analysis confirmed that macrophages are of critical importance and are potential therapeutic targets in RA. Repeated treatment with MA triggered a macrophage phenotypic switch in the paws, with fewer M1 macrophages. Prominent increases in the Treg cell population and TGF-β1 in the popliteal lymph nodes demonstrated the immunomodulatory effects of MA. Furthermore, a selective TGF-β1-receptor inhibitor, SB431542, attenuated the anti-inflammatory effects of MA and MA-induced suppression of the levels of M1-released cytokines.</p><p><strong>Conclusion: </strong>These findings provide novel evidence that the anti-inflammatory and analgesic effects of MA on RA act through phenotypic modulation involving the inhibition of M1 macrophage polarization and an increase in the Treg cell population, highlighting the potential therapeutic advantages of acupuncture in controlling pain and ameliorating inflammatory conditions.</p>","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":"41 2","pages":"96-109"},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An audit of structure-based medical acupuncture by a single provider in patients with musculoskeletal pain using PROMIS scores as the outcome.","authors":"Chi-Tsai Tang,&nbsp;Michael Sookochoff,&nbsp;Lee Rhea,&nbsp;Jonathan Carrier,&nbsp;Heidi Prather,&nbsp;Ling Guan","doi":"10.1177/09645284221118589","DOIUrl":"https://doi.org/10.1177/09645284221118589","url":null,"abstract":"<p><strong>Background: </strong>To assess whether structure-based medical acupuncture (SMA) can improve Patient-Reported Outcomes Measurement Information System (PROMIS) scores in patients presenting with musculoskeletal pain.</p><p><strong>Methods: </strong>An audit was conducted of all patients presenting with musculoskeletal pain treated by a single provider with SMA in 2017. Inclusion criteria included a pre-treatment and at least one post-treatment PROMIS score. Patient demographics and previous treatments tried were recorded. Documented events other than acupuncture that were thought to interfere with PROMIS scores were recorded, and no further scores were used after these events. A maximum of nine visits after the initial visit were used. The PROMIS domains assessed included anxiety, depression, pain interference and physical function.</p><p><strong>Results: </strong>Seventy-two patients who had been treated with SMA met the inclusion criteria. Sixty-five of the patients (90%) had chronic pain. For their presenting complaint, 59 (82%) had previously sought treatment from another non-operative provider, 60 (83%) had tried physical therapy, and 20 (28%) had even had surgery. Despite this, SMA appeared to be able to significantly improve PROMIS anxiety at visits 1-3 and PROMIS depression at visit 3. After just one treatment, minimal clinically important differences (MCID) were reached in 32%-44% of patients for PROMIS anxiety, 17%-36% for PROMIS depression, 28%-29% for PROMIS physical function, and 21%-36% for PROMIS pain interference, based on low and high cut-offs of a range of quoted MCID values.</p><p><strong>Conclusion: </strong>In a difficult patient population with musculoskeletal pain, SMA is a technique that can likely be used to improve PROMIS anxiety and depression, although no firm conclusions can be drawn from this uncontrolled clinical audit. Of note, MCIDs were sometimes obtained even after just one treatment.</p>","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":"41 1","pages":"48-54"},"PeriodicalIF":2.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10536011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of acupuncture as a complementary therapy for sepsis: a systematic review and meta-analysis.","authors":"Jin Xian,&nbsp;Ling Wang,&nbsp;Changyun Zhang,&nbsp;Jian Wang,&nbsp;Yushuo Zhu,&nbsp;Huijuan Yu,&nbsp;Xin Zhang,&nbsp;Qiwen Tan","doi":"10.1177/09645284221086288","DOIUrl":"https://doi.org/10.1177/09645284221086288","url":null,"abstract":"<p><strong>Background: </strong>Sepsis is a life-threatening organ dysfunction caused by dysregulation of the host response to infection. Acupuncture is used for treatment of inflammatory diseases; however, its effectiveness and safety as a complementary therapy for sepsis has not been fully explored.</p><p><strong>Methods: </strong>Data were retrieved from eight databases. Randomized controlled trials (RCTs) that compared acupuncture plus conventional therapies versus conventional therapies alone were included. Pre-specified primary outcomes were mortality at 28 days and Acute Physiologic and Chronic Health Evaluation (APACHE) II scores.</p><p><strong>Results: </strong>A total of 17 studies with 1099 participants were included in this study. In terms of the primary outcomes, acupuncture plus routine therapy reduced mortality at 28 days (risk ratio (RR)): 0.69, 95% confidence interval (CI): 0.52 to 0.91, p < 0.001) and APACHE II scores (mean difference (MD): -2.84, 95% CI: -4.09 to -1.58, p < 0.001) at day 7 after treatment compared with routine therapy alone. In terms of secondary outcomes, acupuncture plus routine therapy reduced white blood cell counts and levels of procalcitonin (PCT), tumor necrosis factor (TNF)-α, interleukin (IL)-6 and lactic acid and intra-abdominal pressure (IAP), and improved CD3+, CD4+ and monocytes of human leukocyte antigen (HLA)-DR at day 7 after treatment compared with routine therapy alone. However, acupuncture plus routine therapy had no significant effects on levels of IL-10, C-reactive protein (CRP), CD8+ and CD4+/CD8+ ratios compared with routine therapy alone. Quality of evidence was low to very low for all parameters (GRADE).</p><p><strong>Conclusion: </strong>The available evidence showed that combination of acupuncture and routine therapy may have benefit for sepsis compared with use of routine therapy only. Due to the low degree of certainty regarding its effects, further research is required.</p><p><strong>Trial registration number: </strong>ICRD42019141491 (PROSPERO).</p>","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":"41 1","pages":"3-15"},"PeriodicalIF":2.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10537568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Health professionals' attitudes towards acupuncture/acupressure for post-operative nausea and vomiting: a survey and implications for implementation.","authors":"Zhen Zheng,&nbsp;Wanda S Stelmach,&nbsp;Jason Ma,&nbsp;Juris Briedis,&nbsp;Raphael Hau,&nbsp;Mark Tacey,&nbsp;Jeannette Atme,&nbsp;Debra Bourne,&nbsp;Julie Crabbe,&nbsp;Catherine Fletcher,&nbsp;Paul Howat,&nbsp;Jenny Layton,&nbsp;Charlie C Xue","doi":"10.1177/09645284221085282","DOIUrl":"https://doi.org/10.1177/09645284221085282","url":null,"abstract":"<p><strong>Purpose: </strong>Level 1 evidence supports the use of acupuncture/acupressure (A/A) to manage post-operative nausea and vomiting (PONV). This study aimed to survey healthcare professionals' attitudes towards A/A, influencing factors and barriers to implementing this effective non-drug intervention into peri-operative care.</p><p><strong>Methods: </strong>A validated, anonymous survey with 43 questions was emailed or distributed as a hard copy at meetings to anaesthetists, midwives, nurses, obstetricians, gynaecologists and surgeons at a public hospital in Australia. Descriptive data were presented. Influencing factors were explored using chi-square analysis. Multinomial logistical regression was used to identify the influences of confounding factors.</p><p><strong>Results: </strong>A total of 155 completed surveys were returned, reflecting a response rate of 32%. The majority of participants were female (69%), nurses/midwives (61%) and aged between 20 and 50 years old (76%). Eighty-three percent of respondents considered A/A 'clearly alternative' medicine or 'neither mainstream nor alternative'. Eighty-one percent would encourage patients to use acupressure for PONV if it was offered at the hospital. Previous personal use of A/A was the key factor influencing attitudes and openness to clinical use. The key barriers to implementation were perceived lack of evidence and lack of qualified providers and time.</p><p><strong>Conclusion: </strong>Hospital-based healthcare professionals strongly supported the evidence-based use of A/A for PONV despite considering the therapy to be non-mainstream and having limited A/A education or history of personal use, providing a positive context for an acupressure implementation study. Significant gaps in training and a desire to learn were identified.</p>","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":"41 1","pages":"16-26"},"PeriodicalIF":2.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10537567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture following deep needle insertion at BL39 and BL40 improves acute anterior cruciate ligament injury: a case report.","authors":"Cong Deng,&nbsp;Haishan Zheng,&nbsp;Xiaolin Zhuo,&nbsp;Jinxiong Lao","doi":"10.1177/09645284221125251","DOIUrl":"https://doi.org/10.1177/09645284221125251","url":null,"abstract":"Typically, due to inaccurate posture and force, the anterior cruciate ligament (ACL) often tears in acute knee injuries. Clinically, bone marrow edema (BME) and popliteus injuries often appear in patients with ACL tears but tend to be masked by symptoms of ACL injury. To diagnose BME or injuries of the ACL or popliteus, the most commonly recommended examination is magnetic resonance imaging (MRI).1–3 Reconstructive repair and physical rehabilitation are mainstream treatments. However, patients with combined ACL and popliteus injuries are at increased risk of reconstruction failure.3 Treatments aim to shorten the clinical course and relieve pain.2 However, no targeted medications are currently available for patients with coexisting traumatic BME. Recently, acupuncture has shown to be effective in the treatment of knee osteoarthritis, while there are few reports of acupuncture for acute ACL tears. Here, we report a case of a patient with an acute partial tear of the ACL combined with BME and damage to popliteus who improved after treatment with acupuncture.","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":"41 1","pages":"58-60"},"PeriodicalIF":2.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9088673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acupuncture for female sexual dysfunction: a case report.","authors":"Sen-Lin Ye,&nbsp;Yue Feng,&nbsp;Ru-Yi Zhou,&nbsp;Cai-Gui Luo","doi":"10.1177/09645284221125427","DOIUrl":"https://doi.org/10.1177/09645284221125427","url":null,"abstract":"Female sexual dysfunction (FSD) refers to the inability of women to meet their own expectations with respect to their sex lives, resulting in a lack of satisfaction. Typical symptoms include low libido or difficulty with arousal, orgasmic disorders, or painful intercourse. A meta-analysis showed that the prevalence of FSD in premenopausal women is approximately 40.9%, and the prevalence of individual sexual dysfunction is 20.6%–28.2%.1 The most effective treatment method is hormone therapy,2 but this might have detrimental side effects. Although FSD is not life-threatening, it can have a huge negative impact on quality of life. Patients with FSD have a higher prevalence of anxiety and depression than unaffected individuals.3 Accordingly, alternative treatment options with fewer potential side-effects are desirable. Acupuncture, which has minimal side-effects, is widely used in diseases of the genitourinary system. However, there is currently limited evidence to support acupuncture in the treatment of FSD. Here, we describe a case in which a patient with FSD seemed to be successfully treated by acupuncture.","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":"41 1","pages":"55-57"},"PeriodicalIF":2.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10539222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture attenuates ac4C modification of P16 mRNA in the ovarian granulosa cells of a mouse model premature ovarian failure.","authors":"Zixiang Geng,&nbsp;Peng Liu,&nbsp;Long Yuan,&nbsp;Kaiyong Zhang,&nbsp;Jiajia Lin,&nbsp;Xiaoli Nie,&nbsp;Huiru Jiang,&nbsp;Bingrong Li,&nbsp;Te Liu,&nbsp;Bimeng Zhang","doi":"10.1177/09645284221085284","DOIUrl":"https://doi.org/10.1177/09645284221085284","url":null,"abstract":"<p><strong>Background: </strong>Premature ovarian failure (POF) is a type of pathological aging, which seriously interferes with the fertility of affected women. Electroacupuncture (EA) may have a beneficial effect; however, its mechanism of action is unknown. The purpose of this study was to determine the effect of EA on ovarian function in ovarian granulosa cells (OGCs) in a cyclophosphamide (CTX)-induced mouse model of POF.</p><p><strong>Methods: </strong>Mice were divided into three groups: wild type (WT) group, CTX group and CTX + EA group. EA was administered under isoflurane anesthesia at CV4, ST36 and SP6 for 30 min every 2 days, 2-3 times per week for a total of 4 weeks. Effects of EA on ovarian weight and level of estrogen were examined. The mRNA and protein expression levels of cell cycle-associated proteins were detected and mRNA modifications were analyzed.</p><p><strong>Results: </strong>EA significantly increased ovarian weight and reduced the proportion of atretic follicles in mice with CTX-induced POF (p < 0.05). EA increased the level of estrogen in the peripheral blood of mice and inhibited the modification of total mRNA N4-acetylcytidine (ac4C). A significant increase in the expression of P16 and N-acetyltransferase 10 (NAT10) and a significant decrease in the expression of Cyclin D (CCND1) and cyclin-dependent kinase 6 (CDK6) were observed in the OGCs of POF mice (p<0.05). After EA, P16 and NAT10 expression was decreased, and CCND1 and CDK6 expression was increased. Finally, EA reduced the ac4C modification of P16 mRNA-specific sites in the OGCs of POF mice.</p><p><strong>Conclusion: </strong>This study demonstrated that EA promoted the repair of the ovarian microenvironment by inhibiting the ac4C modification of P16 mRNA to decrease its stability and expression intensity, and by altering the activity of the P16/CDK6/CCND1 axis in OGCs.</p>","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":"41 1","pages":"27-37"},"PeriodicalIF":2.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10537134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}