Electroacupuncture improves follicular development and metabolism and regulates the expression of adiponectin, AMPK and ACC in an obese rat model of polycystic ovary syndrome.

IF 2.4 3区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Acupuncture in Medicine Pub Date : 2023-06-01 DOI:10.1177/09645284221107690

Jing Zhou, Ping Yin, Qingyi Zhao, Zhihai Hu, Yi Wang, Guizhi Ma, Xinyi Wu, Lu Lu, Yin Shi

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引用次数: 0

Abstract

Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder characterized by hyperandrogenism and follicular arrest. Electroacupuncture (EA) has been shown to be effective at improving hyperandrogenism and follicular arrest in PCOS; however, its mechanism of action remains to be deciphered.

Objective: In this study, we investigated whether EA improved follicular development in an obese rat model of PCOS and regulated the expression of adiponectin, AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC).

Methods: EA was administered at CV3, CV4 and ST40. Changes in body weight, paraovarian fat, estrus cycle, ovarian morphology, levels of related hormones, and glucose and lipid metabolism were evaluated. In addition, protein and mRNA expression of adiponectin, AMPK and ACC was measured.

Results: The body weight and paraovarian fat of rats in the EA group were reduced, while estrus cyclicity and ovarian morphology improved. Levels of free fatty acids, triglycerides, total cholesterol and low-density lipoprotein cholesterol were significantly reduced in the EA group, as well as blood glucose levels. Furthermore, levels of testosterone and luteinizing hormone were reduced in the EA group, while estradiol levels were increased. Protein and mRNA expression of adiponectin, AMPKα1 and liver kinase B1 (LKB1) was found to be increased in the EA group, while protein and mRNA expression of ACC were significantly reduced.Conclusion: Our findings suggest that EA improved follicular development and metabolism and regulated expression levels of adiponectin, AMPKα1, LKB1 and ACC in our obese rat model of PCOS.

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电针改善多囊卵巢综合征肥胖大鼠模型卵泡发育和代谢，调节脂联素、AMPK和ACC的表达。

背景:多囊卵巢综合征(PCOS)是一种常见的内分泌疾病，以雄激素分泌过多和卵泡停止为特征。电针(EA)已被证明对改善多囊卵巢综合征的高雄激素症和卵泡骤停有效;然而，其作用机制仍有待研究。目的:本研究探讨EA是否能改善肥胖大鼠PCOS模型的卵泡发育，并调节脂联素、amp活化蛋白激酶(AMPK)和乙酰辅酶a羧化酶(ACC)的表达。方法:对CV3、CV4和ST40进行EA治疗。评估体重、卵巢旁脂肪、发情周期、卵巢形态、相关激素水平以及糖脂代谢的变化。测定脂联素、AMPK、ACC蛋白和mRNA的表达。结果:EA组大鼠体重减轻，卵巢旁脂肪减少，发情周期和卵巢形态改善。EA组的游离脂肪酸、甘油三酯、总胆固醇和低密度脂蛋白胆固醇水平以及血糖水平均显著降低。此外，EA组的睾酮和黄体生成素水平降低，而雌二醇水平升高。EA组脂联素、AMPKα1和肝激酶B1 (LKB1)蛋白和mRNA表达升高，ACC蛋白和mRNA表达显著降低。结论:我们的研究结果表明，EA可以改善PCOS肥胖大鼠的卵泡发育和代谢，调节脂联素、AMPKα1、LKB1和ACC的表达水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acupuncture in Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

4.70

自引率

4.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Acupuncture in Medicine aims to promote the scientific understanding of acupuncture and related treatments by publishing scientific investigations of their effectiveness and modes of action as well as articles on their use in health services and clinical practice. Acupuncture in Medicine uses the Western understanding of neurophysiology and anatomy to interpret the effects of acupuncture.