Cell insight最新文献_第5页

The role of TRPV4 in acute sleep deprivation-induced memory impairment: Mechanisms of calcium dysregulation and synaptic plasticity disruption TRPV4在急性睡眠剥夺引起的记忆障碍中的作用：钙调节失调和突触可塑性破坏的机制

Cell insight Pub Date : 2025-06-01 Epub Date: 2025-03-31 DOI: 10.1016/j.cellin.2025.100240

Meimei Guo , Feiyang Zhang , Sha Liu , Yi Zhang , Lesheng Wang , Jian Song , Wei Wei , Xiang Li

{"title":"The role of TRPV4 in acute sleep deprivation-induced memory impairment: Mechanisms of calcium dysregulation and synaptic plasticity disruption","authors":"Meimei Guo , Feiyang Zhang , Sha Liu , Yi Zhang , Lesheng Wang , Jian Song , Wei Wei , Xiang Li","doi":"10.1016/j.cellin.2025.100240","DOIUrl":"10.1016/j.cellin.2025.100240","url":null,"abstract":"<div><div>Acute sleep deprivation (ASD) impairs memory formation, but the underlying mechanisms remain unclear. In this study, we employed an ASD model combined with fear conditioning to investigate these mechanisms. mRNA sequencing revealed upregulated expression of Transient Receptor Potential Vanilloid 4 (TRPV4), a nonselective Ca<sup>2+</sup>-permeable cation channel critical for calcium signaling, in mice with ASD-induced memory impairments. Notably, TRPV4 knockdown reversed ASD-induced memory deficits. ASD was associated with increased intracellular Ca<sup>2+</sup> concentrations, reduced spine density, and decreased expression of postsynaptic density protein 95 (PSD95), a key regulator of synaptic plasticity. These findings suggest that ASD may cause Ca<sup>2+</sup> overload, leading to disrupted synaptic plasticity and impaired learning and memory. Importantly, TRPV4 knockdown significantly reduced Ca<sup>2+</sup> concentrations, mitigated synaptic plasticity impairments, and contributed to memory restoration. Together, these findings demonstrate a protective role of TRPV4 knockdown against ASD-induced memory deficits and highlight TRPV4 as a potential therapeutic target for memory impairment associated with ASD.</div></div>","PeriodicalId":72541,"journal":{"name":"Cell insight","volume":"4 3","pages":"Article 100240"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precision epitope editing: A path to advanced immunotherapies 精确表位编辑：先进免疫疗法的途径

Cell insight Pub Date : 2025-04-01 Epub Date: 2024-12-24 DOI: 10.1016/j.cellin.2024.100226

Rui-Jin Ji , Mu-Yao Wang , Ying Zhang

引用次数: 0

Cover 封面

Cell insight Pub Date : 2025-04-01 Epub Date: 2025-04-11 DOI: 10.1016/S2772-8927(25)00015-X

引用次数: 0

Inflammatory signatures of microglia in hypercortisolemia-related depression 高皮质醇相关性抑郁症中小胶质细胞的炎症特征。

Cell insight Pub Date : 2025-04-01 Epub Date: 2024-11-13 DOI: 10.1016/j.cellin.2024.100222

Yanxiang Zhao , Yingying Huang , Zhangyuzi Deng, Ying Cao, Jing Yang

引用次数: 0

Corrigendum to previous published articles 以前发表的文章的更正。

Cell insight Pub Date : 2025-04-01 Epub Date: 2025-01-11 DOI: 10.1016/j.cellin.2024.100225

引用次数: 0

The role of colonic microbiota amino acid metabolism in gut health regulation 结肠菌群氨基酸代谢在肠道健康调节中的作用

Cell insight Pub Date : 2025-04-01 Epub Date: 2025-01-10 DOI: 10.1016/j.cellin.2025.100227

Youli Chen, Jing-Yuan Fang

引用次数: 0

The promising role of nanopore sequencing in cancer diagnostics and treatment 纳米孔测序在癌症诊断和治疗中的重要作用

Cell insight Pub Date : 2025-04-01 Epub Date: 2025-01-18 DOI: 10.1016/j.cellin.2025.100229

Xinming Su , Qingyuan Lin , Bin Liu , Chuntao Zhou , Liuyi Lu , Zihao Lin , Jiahua Si , Yuemin Ding , Shiwei Duan

{"title":"The promising role of nanopore sequencing in cancer diagnostics and treatment","authors":"Xinming Su , Qingyuan Lin , Bin Liu , Chuntao Zhou , Liuyi Lu , Zihao Lin , Jiahua Si , Yuemin Ding , Shiwei Duan","doi":"10.1016/j.cellin.2025.100229","DOIUrl":"10.1016/j.cellin.2025.100229","url":null,"abstract":"<div><div>Cancer arises from genetic alterations that impact both the genome and transcriptome. The utilization of nanopore sequencing offers a powerful means of detecting these alterations due to its unique capacity for long single-molecule sequencing. In the context of DNA analysis, nanopore sequencing excels in identifying structural variations (SVs), copy number variations (CNVs), gene fusions within SVs, and mutations in specific genes, including those involving DNA modifications and DNA adducts. In the field of RNA research, nanopore sequencing proves invaluable in discerning differentially expressed transcripts, uncovering novel elements linked to transcriptional regulation, and identifying alternative splicing events and RNA modifications at the single-molecule level. Furthermore, nanopore sequencing extends its reach to detecting microorganisms, encompassing bacteria and viruses, that are intricately associated with tumorigenesis and the development of cancer. Consequently, the application prospects of nanopore sequencing in tumor diagnosis and personalized treatment are expansive, encompassing tasks such as tumor identification and classification, the tailoring of treatment strategies, and the screening of prospective patients. In essence, this technology stands poised to unearth novel mechanisms underlying tumorigenesis while providing dependable support for the diagnosis and treatment of cancer.</div></div>","PeriodicalId":72541,"journal":{"name":"Cell insight","volume":"4 2","pages":"Article 100229"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143265220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-Guided design of Cas12a variants improves detection of nucleic acids Cas12a变异体的结构导向设计提高了核酸的检测

Cell insight Pub Date : 2025-04-01 Epub Date: 2025-01-20 DOI: 10.1016/j.cellin.2025.100228

Xiaohan Tong , Tianle Li , Kun Zhang , Dongming Zhao , Ying Zhang , Hao Yin

引用次数: 0

Unveiling racial disparities in prostate cancer using an integrative genomic and transcriptomic analysis 利用综合基因组学和转录组学分析揭示前列腺癌的种族差异

Cell insight Pub Date : 2025-04-01 Epub Date: 2025-02-17 DOI: 10.1016/j.cellin.2025.100238

Abdalla Elbialy , Akshay Sood , Shang-Jui Wang , Peng Wang , Ahmed Fadiel , Anil V. Parwani , Steven Huang , Gennady Shvets , Nagireddy Putluri , Jenny Li , Xuefeng Liu

{"title":"Unveiling racial disparities in prostate cancer using an integrative genomic and transcriptomic analysis","authors":"Abdalla Elbialy , Akshay Sood , Shang-Jui Wang , Peng Wang , Ahmed Fadiel , Anil V. Parwani , Steven Huang , Gennady Shvets , Nagireddy Putluri , Jenny Li , Xuefeng Liu","doi":"10.1016/j.cellin.2025.100238","DOIUrl":"10.1016/j.cellin.2025.100238","url":null,"abstract":"<div><div>Prostate cancer exhibits significant racial disparities, with African American (AA) individuals showing ∼64% higher incidence and 2.3 times greater mortality rates compared to their Caucasian (CA) counterparts. Understanding the complex interplay of genetic, environmental, lifestyle, socioeconomic, and healthcare access factors is crucial for developing effective interventions to reduce this disproportionate burden.</div><div>This study aims to uncover the genetic and transcriptomic differences driving these disparities through a comprehensive analysis using RNA sequencing (RNA-seq) and exome sequencing of prostate cancer tissues from both Black and White patients.</div><div>Our transcriptomics analysis revealed enhanced activity in pathways linked to immune response and cellular interactions in AA prostate cancer samples, with notable regulation by histone-associated transcription factors (HIST1H1A, HIST1H1D, and HIST1H1B) suggests potential involvement of histone modification mechanisms. Additionally, pseudogenes and long non-coding RNAs (lncRNAs) among the regulated genes indicate non-coding elements' role in these disparities.</div><div>Exome sequencing identified unique variants in AA patient samples within key genes, including TP73 (tumor suppression), XYLB (metabolism), ALDH4A1 (oxidative stress), PTPRB (cellular signaling), and HLA-DRB5 (immune response). These genetic variations likely contribute to disease progression and therapy response disparities.</div><div>This study highlights the importance of considering genetic and epigenetic variations in developing tailored therapeutic approaches to improve treatment efficacy and reduce mortality rates across diverse populations.</div></div>","PeriodicalId":72541,"journal":{"name":"Cell insight","volume":"4 2","pages":"Article 100238"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The interplay between Salmonella and host: Mechanisms and strategies for bacterial survival 沙门氏菌与宿主之间的相互作用：细菌生存的机制和策略

Cell insight Pub Date : 2025-04-01 Epub Date: 2025-02-13 DOI: 10.1016/j.cellin.2025.100237

Hongyu Zhao , Xinyue Zhang , Ningning Zhang , Li Zhu , Huan Lian

{"title":"The interplay between Salmonella and host: Mechanisms and strategies for bacterial survival","authors":"Hongyu Zhao , Xinyue Zhang , Ningning Zhang , Li Zhu , Huan Lian","doi":"10.1016/j.cellin.2025.100237","DOIUrl":"10.1016/j.cellin.2025.100237","url":null,"abstract":"<div><div>Salmonella, an intracellular pathogen, infects both humans and animals, causing diverse diseases such as gastroenteritis and enteric fever. The <em>Salmonella</em> type III secretion system (T3SS), encoded within its pathogenicity islands (SPIs), is critical for bacterial virulence by directly delivering multiple effectors into eukaryotic host cells. <em>Salmonella</em> utilizes these effectors to facilitate its survival and replication within the host through modulating cytoskeletal dynamics, inflammatory responses, the biogenesis of <em>Salmonella</em>-containing vacuole (SCV), and host cell survival. Moreover, these effectors also interfere with immune responses via inhibiting innate immunity or antigen presentation. In this review, we summarize the current progress in the survival strategies employed by <em>Salmonella</em> and the molecular mechanisms underlying its interactions with the host. Understanding the interplay between <em>Salmonella</em> and host can enhance our knowledge of the bacterium's pathogenic processes and provide new insights into how it manipulates host cellular physiological activities to ensure its survival.</div></div>","PeriodicalId":72541,"journal":{"name":"Cell insight","volume":"4 2","pages":"Article 100237"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0