Cell insight最新文献_第10页

The role of TRPA1 channels in thermosensation TRPA1通道在热感觉中的作用

Cell insight Pub Date : 2022-12-01 DOI: 10.1016/j.cellin.2022.100059

Hao Zhang , Chengsan Wang , Keyi Zhang , Peter Muiruri Kamau , Anna Luo , Lifeng Tian , Ren Lai

引用次数: 3

Current advances of CRISPR-Cas technology in cell therapy CRISPR-Cas技术在细胞治疗中的最新进展

Cell insight Pub Date : 2022-12-01 DOI: 10.1016/j.cellin.2022.100067

Hou-Yuan Qiu, Rui-Jin Ji, Ying Zhang

引用次数: 7

Cover 封面

Cell insight Pub Date : 2022-12-01 DOI: 10.1016/S2772-8927(22)00066-9

引用次数: 0

Direct cardiac reprogramming: Toward the era of multi-omics analysis 直接心脏重编程:迈向多组学分析时代

Cell insight Pub Date : 2022-12-01 DOI: 10.1016/j.cellin.2022.100058

Mengxin Liu , Jie Liu , Tong Zhang , Li Wang

引用次数: 0

A selective degeneration of cholinergic neurons mediated by NRADD in an Alzheimer's disease mouse model NRADD介导的阿尔茨海默病小鼠模型胆碱能神经元选择性变性

Cell insight Pub Date : 2022-12-01 DOI: 10.1016/j.cellin.2022.100060

Lanfang Li , Bing Zhang , Xiaomei Tang , Quntao Yu , Aodi He , Youming Lu , Xinyan Li

{"title":"A selective degeneration of cholinergic neurons mediated by NRADD in an Alzheimer's disease mouse model","authors":"Lanfang Li , Bing Zhang , Xiaomei Tang , Quntao Yu , Aodi He , Youming Lu , Xinyan Li","doi":"10.1016/j.cellin.2022.100060","DOIUrl":"10.1016/j.cellin.2022.100060","url":null,"abstract":"<div><p>Cholinergic neurons in the basal forebrain constitute a major source of cholinergic inputs to the forebrain, modulate diverse functions including sensory processing, memory and attention, and are vulnerable to Alzheimer's disease (AD). Recently, we classified cholinergic neurons into two distinct subpopulations; calbindin D28K-expressing (D28K<sup>+</sup>) versus D28K-lacking (D28K<sup>−</sup>) neurons. Yet, which of these two cholinergic subpopulations are selectively degenerated in AD and the molecular mechanisms underlying this selective degeneration remain unknown. Here, we reported a discovery that D28K<sup>+</sup> neurons are selectively degenerated and this degeneration induces anxiety-like behaviors in the early stage of AD. Neuronal type specific deletion of NRADD effectively rescues D28K<sup>+</sup> neuronal degeneration, whereas genetic introduction of exogenous NRADD causes D28K<sup>−</sup> neuronal loss. This gain- and loss-of-function study reveals a subtype specific degeneration of cholinergic neurons in the disease progression of AD and hence warrants a novel molecular target for AD therapy.</p></div>","PeriodicalId":72541,"journal":{"name":"Cell insight","volume":"1 6","pages":"Article 100060"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10120297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9540883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Trim25 restricts rabies virus replication by destabilizing phosphoprotein Trim25通过破坏磷酸化蛋白的稳定来限制狂犬病毒的复制

Cell insight Pub Date : 2022-10-01 DOI: 10.1016/j.cellin.2022.100057

Yueming Yuan , An Fang , Zongmei Wang , Bin Tian , Yuan Zhang , Baokun Sui , Zhaochen Luo , Yingying Li , Ming Zhou , Huanchun Chen , Zhen F. Fu , Ling Zhao

{"title":"Trim25 restricts rabies virus replication by destabilizing phosphoprotein","authors":"Yueming Yuan , An Fang , Zongmei Wang , Bin Tian , Yuan Zhang , Baokun Sui , Zhaochen Luo , Yingying Li , Ming Zhou , Huanchun Chen , Zhen F. Fu , Ling Zhao","doi":"10.1016/j.cellin.2022.100057","DOIUrl":"10.1016/j.cellin.2022.100057","url":null,"abstract":"<div><p>Tripartite motif-containing protein 25 (Trim25) is an E3 ubiquitin ligase that activates retinoid acid-inducible gene I (RIG-I) and promotes the antiviral interferon response. Recent studies have shown that Trim25 can bind and degrade viral proteins, suggesting a different mechanism of Trim25 on its antiviral effects. In this study, Trim25 expression was upregulated in cells and mouse brains after rabies virus (RABV) infection. Moreover, expression of Trim25 limited RABV replication in cultured cells. Overexpression of Trim25 caused attenuated viral pathogenicity in a mouse model that was intramuscularly injected with RABV. Further experiments confirmed that Trim25 inhibited RABV replication via two different mechanisms: an E3 ubiquitin ligase-dependent mechanism and an E3 ubiquitin ligase-independent mechanism. Specifically, the CCD domain of Trim25 interacted with RABV phosphoprotein (RABV-P) at amino acid (AA) position at 72 and impaired the stability of RABV-P via complete autophagy. This study reveals a novel mechanism by which Trim25 restricts RABV replication by destabilizing RABV-P, which is independent of its E3 ubiquitin ligase activity.</p></div>","PeriodicalId":72541,"journal":{"name":"Cell insight","volume":"1 5","pages":"Article 100057"},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cc/ea/main.PMC10120326.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9540900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Endothelial RGS12 governs angiogenesis in inflammatory arthritis by controlling cilia formation and elongation via MYCBP2 signaling 内皮细胞RGS12通过MYCBP2信号控制纤毛的形成和伸长，从而调控炎症性关节炎的血管生成

Cell insight Pub Date : 2022-10-01 DOI: 10.1016/j.cellin.2022.100055

Gongsheng Yuan , Shu-ting Yang , Shuying Yang

{"title":"Endothelial RGS12 governs angiogenesis in inflammatory arthritis by controlling cilia formation and elongation via MYCBP2 signaling","authors":"Gongsheng Yuan , Shu-ting Yang , Shuying Yang","doi":"10.1016/j.cellin.2022.100055","DOIUrl":"10.1016/j.cellin.2022.100055","url":null,"abstract":"<div><p>Angiogenesis is the formation of new capillaries that plays an essential role in the pathogenesis of inflammatory arthritis. However, the cellular and molecular mechanisms remain unclear. Here, we provide the first evidence that regulator of G-protein signaling 12 (RGS12) promotes angiogenesis in inflammatory arthritis through governing ciliogenesis and cilia elongation in endothelial cells. The knockout of RGS12 inhibits the development of inflammatory arthritis with the reduction in clinical score, paw swelling, and angiogenesis. Mechanistically, RGS12 overexpression (OE) in endothelial cells increases cilia number and length, and thereby promotes cell migration and tube-like structure formation. The knockout of cilia marker protein Intraflagellar transport (IFT) 80 blocked the increase in cilia number and length caused by RGS12 OE. Moreover, the results from LC/MS and IP analysis showed that RGS12 is associated with cilia-related protein MYC binding protein 2 (MYCBP2), which enhances the phosphorylation of MYCBP2 to promote ciliogenesis in endothelial cells. These findings demonstrate that upregulation of RGS12 by inflammation enhances angiogenesis by promoting cilia formation and elongation via activation of MYCBP2 signaling during inflammatory arthritis pathogenesis.</p></div>","PeriodicalId":72541,"journal":{"name":"Cell insight","volume":"1 5","pages":"Article 100055"},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/74/68/main.PMC10120324.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9840602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The functions of polycomb group proteins in T cells 多梳蛋白在T细胞中的功能

Cell insight Pub Date : 2022-10-01 DOI: 10.1016/j.cellin.2022.100048

Ting Li

引用次数: 0

mRNA produced by VSW-3 RNAP has high-level translation efficiency with low inflammatory stimulation VSW-3 RNAP产生的mRNA具有高翻译效率和低炎症刺激

Cell insight Pub Date : 2022-10-01 DOI: 10.1016/j.cellin.2022.100056

Guoquan Wang , Rui Cheng , Qiubing Chen , Yuandong Xu , Bingbing Yu , Bin Zhu , Hao Yin , Heng Xia

引用次数: 5

Screening of SARS-CoV-2 antivirals through a cell-based RNA-dependent RNA polymerase (RdRp) reporter assay 基于细胞的RNA依赖性RNA聚合酶(RdRp)报告基因试验筛选SARS-CoV-2抗病毒药物

Cell insight Pub Date : 2022-08-01 DOI: 10.1016/j.cellin.2022.100046

Timsy Uppal , Kai Tuffo , Svetlana Khaiboullina , Sivani Reganti , Mark Pandori , Subhash C. Verma

{"title":"Screening of SARS-CoV-2 antivirals through a cell-based RNA-dependent RNA polymerase (RdRp) reporter assay","authors":"Timsy Uppal , Kai Tuffo , Svetlana Khaiboullina , Sivani Reganti , Mark Pandori , Subhash C. Verma","doi":"10.1016/j.cellin.2022.100046","DOIUrl":"10.1016/j.cellin.2022.100046","url":null,"abstract":"<div><p>COVID-19 (Coronavirus Disease 2019) caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome CoronaVirus-2) continues to pose an international public health threat and thus far, has resulted in greater than 6.4 million deaths worldwide. Vaccines are critical tools to limit COVID-19 spread, but antiviral drug development is an ongoing global priority due to fast-spreading COVID-19 variants that may elude vaccine efficacies. The RNA-dependent RNA polymerase (RdRp) of SARS-CoV-2 is an essential enzyme of viral replication and transcription machinery complex. Therefore, the RdRp is an attractive target for the development of effective anti-COVID-19 therapeutics. In this study, we developed a cell-based assay to determine the enzymatic activity of SARS-CoV-2 RdRp through a luciferase reporter system. The SARS-CoV-2 RdRp reporter assay was validated using known inhibitors of RdRp polymerase, remdesivir along with other anti-virals including ribavirin, penciclovir, rhoifolin, 5′CT, and dasabuvir. Dasabuvir (an FDA-approved drug) exhibited promising RdRp inhibitory activity among these inhibitors. Anti-viral activity of dasabuvir was also tested on the replication of SARS-CoV-2 through infection of Vero E6 cells. Dasabuvir inhibited the replication of SARS-CoV-2, USA-WA1/2020 as well as B.1.617.2 (delta variant) in Vero E6 cells in a dose-dependent manner with EC<sub>50</sub> values 9.47 μM and 10.48 μM, for USA-WA1/2020 and B.1.617.2 variants, respectively. Our results suggest that dasabuvir can be further evaluated as a therapeutic drug for COVID-19. Importantly, this system provides a robust, target-specific, and high-throughput screening compatible (z- and z’-factors of >0.5) platforms that will be a valuable tool for screening SARS-CoV-2 RdRp inhibitors.</p></div>","PeriodicalId":72541,"journal":{"name":"Cell insight","volume":"1 4","pages":"Article 100046"},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d8/14/main.PMC9239919.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9540492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2