Cell insight最新文献_第10页

A role for Hes1 in constraining germinal center B cell formation Hes1在抑制生发中心B细胞形成中的作用

Cell insight Pub Date : 2023-04-01 DOI: 10.1016/j.cellin.2023.100078

Xingxing Shao , Xin Liu , Hai Qi

引用次数: 0

Atlas of interactions between SARS-CoV-2 macromolecules and host proteins SARS-CoV-2大分子与宿主蛋白相互作用图谱

Cell insight Pub Date : 2023-02-01 DOI: 10.1016/j.cellin.2022.100068

Guangnan Li , Zhidong Tang , Weiliang Fan , Xi Wang , Li Huang , Yu Jia , Manli Wang , Zhihong Hu , Yu Zhou

{"title":"Atlas of interactions between SARS-CoV-2 macromolecules and host proteins","authors":"Guangnan Li , Zhidong Tang , Weiliang Fan , Xi Wang , Li Huang , Yu Jia , Manli Wang , Zhihong Hu , Yu Zhou","doi":"10.1016/j.cellin.2022.100068","DOIUrl":"10.1016/j.cellin.2022.100068","url":null,"abstract":"<div><p>The proteins and RNAs of viruses extensively interact with host proteins after infection. We collected and reanalyzed all available datasets of protein-protein and RNA-protein interactions related to SARS-CoV-2. We investigated the reproducibility of those interactions and made strict filters to identify highly confident interactions. We systematically analyzed the interaction network and identified preferred subcellular localizations of viral proteins, some of which such as ORF8 in ER and ORF7A/B in ER membrane were validated using dual fluorescence imaging. Moreover, we showed that viral proteins frequently interact with host machinery related to protein processing in ER and vesicle-associated processes. Integrating the protein- and RNA-interactomes, we found that SARS-CoV-2 RNA and its N protein closely interacted with stress granules including 40 core factors, of which we specifically validated G3BP1, IGF2BP1, and MOV10 using RIP and Co-IP assays. Combining CRISPR screening results, we further identified 86 antiviral and 62 proviral factors and associated drugs. Using network diffusion, we found additional 44 interacting proteins including two proviral factors previously validated. Furthermore, we showed that this atlas could be applied to identify the complications associated with COVID-19. All data are available in the AIMaP database (<span>https://mvip.whu.edu.cn/aimap/</span><svg><path></path></svg>) for users to easily explore the interaction map.</p></div>","PeriodicalId":72541,"journal":{"name":"Cell insight","volume":"2 1","pages":"Article 100068"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1e/e4/main.PMC9670597.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9485001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Hexokinases in cancer and other pathologies 癌症和其他疾病中的己糖激酶。

Cell insight Pub Date : 2023-02-01 DOI: 10.1016/j.cellin.2023.100077

Dong Guo , Ying Meng , Xiaoming Jiang , Zhimin Lu

引用次数: 9

Structural basis of the TCR-pHLA complex provides insights into the unconventional recognition of CDR3β in TCR cross-reactivity and alloreactivity TCR-pHLA复合物的结构基础为CDR3β在TCR交叉反应性和同种反应性中的非常规识别提供了见解。

Cell insight Pub Date : 2023-02-01 DOI: 10.1016/j.cellin.2022.100076

Dan San , Jun Lei , Yang Liu , Baowei Jing, Xiang Ye, Pengcheng Wei, Chonil Paek, Yi Yang, Jin Zhou, Peng Chen, Hongjian Wang, Yongshun Chen, Lei Yin

{"title":"Structural basis of the TCR-pHLA complex provides insights into the unconventional recognition of CDR3β in TCR cross-reactivity and alloreactivity","authors":"Dan San , Jun Lei , Yang Liu , Baowei Jing, Xiang Ye, Pengcheng Wei, Chonil Paek, Yi Yang, Jin Zhou, Peng Chen, Hongjian Wang, Yongshun Chen, Lei Yin","doi":"10.1016/j.cellin.2022.100076","DOIUrl":"10.1016/j.cellin.2022.100076","url":null,"abstract":"<div><p>Evidence shows that some class I human leucocyte antigen (HLA) alleles are related to durable HIV controls. The T18A TCR, which has the alloreactivity between HLA-B∗42:01 and HLA-B∗81:01 and the cross-reactivity with different antigen mutants, can sustain long-term HIV controls. Here the structural basis of the T18A TCR binding to the immunodominant HIV epitope TL9 (TPQDLNTML180-188) presented by HLA-B∗42:01 was determined and compared to T18A TCR binding to the TL9 presented by the allo-HLA-B∗81:01. For differences between HLA-B∗42:01 and HLA-B∗81:01, the CDR1α and CDR3α loops adopt a small rearrangement to accommodate them. For different conformations of the TL9 presented by different HLA alleles, not like the conventional recognition of CDR3s to interact with peptide antigens, CDR3β of the T18A TCR shifts to avoid the peptide antigen but intensively recognizes the HLA only, which is different with other conventional TCR structures. Featured sequence pairs of CDR3β and HLA might account for this and were additionally found in multiple other diseases indicating the popularity of the unconventional recognition pattern which would give insights into the control of diseases with epitope mutating such as HIV.</p></div>","PeriodicalId":72541,"journal":{"name":"Cell insight","volume":"2 1","pages":"Article 100076"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/58/27/main.PMC10120306.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9490704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intertwined regulation between RNA m6A modification and cancer metabolism RNA m6A修饰与癌症代谢相互交织的调控

Cell insight Pub Date : 2023-02-01 DOI: 10.1016/j.cellin.2022.100075

Jiaxu Liu , Hao Huang , Minghao Zhang , Guoliang Qing , Hudan Liu

{"title":"Intertwined regulation between RNA m6A modification and cancer metabolism","authors":"Jiaxu Liu , Hao Huang , Minghao Zhang , Guoliang Qing , Hudan Liu","doi":"10.1016/j.cellin.2022.100075","DOIUrl":"https://doi.org/10.1016/j.cellin.2022.100075","url":null,"abstract":"<div><p>RNA N6-methyladenosine (m<sup>6</sup>A) has been identified as the most common, abundant and conserved internal modification in RNA transcripts, especially within eukaryotic messenger RNAs (mRNAs). Accumulating evidence demonstrates that RNA m<sup>6</sup>A modification exploits a wide range of regulatory mechanisms to control gene expression in pathophysiological processes including cancer. Metabolic reprogramming has been widely recognized as a hallmark of cancer. Cancer cells obtain metabolic adaptation through a variety of endogenous and exogenous signaling pathways to promote cell growth and survival in the microenvironment with limited nutrient supply. Recent emerging evidence reveals reciprocal regulation between the m<sup>6</sup>A modification and disordered metabolic events in cancer cells, adding more complexity in the cellular network of metabolic rewiring. In this review, we summarize the most recent advances of how RNA methylation affects tumor metabolism and the feedback regulation of m<sup>6</sup>A modification by metabolic intermediates. We aim to highlight the important connection between RNA m<sup>6</sup>A modification and cancer metabolism, and expect that studise of RNA m<sup>6</sup>A and metabolic reprogramming will lead to greater understanding of cancer pathology.</p></div>","PeriodicalId":72541,"journal":{"name":"Cell insight","volume":"2 1","pages":"Article 100075"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49774143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cover 封面

Cell insight Pub Date : 2023-02-01 DOI: 10.1016/S2772-8927(23)00005-6

引用次数: 0

The role of TRPA1 channels in thermosensation TRPA1通道在热感觉中的作用

Cell insight Pub Date : 2022-12-01 DOI: 10.1016/j.cellin.2022.100059

Hao Zhang , Chengsan Wang , Keyi Zhang , Peter Muiruri Kamau , Anna Luo , Lifeng Tian , Ren Lai

引用次数: 3

Current advances of CRISPR-Cas technology in cell therapy CRISPR-Cas技术在细胞治疗中的最新进展

Cell insight Pub Date : 2022-12-01 DOI: 10.1016/j.cellin.2022.100067

Hou-Yuan Qiu, Rui-Jin Ji, Ying Zhang

引用次数: 7