Acta veterinaria Hungarica最新文献

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Long-term use of an implantable loop recorder for ECG recording in horses. 长期使用植入式循环记录器记录马的心电图。
IF 0.7 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2024-08-22 Print Date: 2024-09-12 DOI: 10.1556/004.2024.01075
Szilvia Kovács, Gábor Bodó, Ferenc Zs Tóth, László Néder, Zoltán Bakos
{"title":"Long-term use of an implantable loop recorder for ECG recording in horses.","authors":"Szilvia Kovács, Gábor Bodó, Ferenc Zs Tóth, László Néder, Zoltán Bakos","doi":"10.1556/004.2024.01075","DOIUrl":"10.1556/004.2024.01075","url":null,"abstract":"<p><p>Potential arrhythmias recorded by cardiac monitors using the built-in human algorithm are as follows: total number of episodes, ventricular tachycardia, asystole, bradycardia, atrial tachycardia, atrial fibrillation and the percentage of time spent in atrial tachycardia or atrial fibrillation. Long-term use of an insertable cardiac monitor (loop recorder) has been reported in one horse earlier, but its accurate role as a diagnostic tool is still unclear. The aim of the present study was to investigate the long-term applicability of the Reveal XT cardiac monitor for recording cardiac arrhythmias in adult horses. The Reveal XT cardiac monitor was implanted in 12 horses under sedation in standing position. Median duration of data recording calculated for the population was 1,169 days. The number of false positive detections of asystole and bradycardia was extremely high in all horses. For atrial fibrillation, false positive detection occurred in five and false negative detection occurred in one horse. The present study showed that the long-term use of the Reveal XT cardiac monitor is feasible, well-tolerated and the devices work reliably, without complications. The human algorithm could not be used for automatic detection of arrhythmic episodes in the study population. The device could detect atrial fibrillation in horses, but the recorded AF burden was inaccurate and the stored ECGs had to be manually interpreted. Because the human analyser algorithm of this cardiac monitor fails to identify bradyarrhythmias in horses based on our results, this instrument is not capable for accurately determining the aetiology of episodic collapse in this species.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"178-185"},"PeriodicalIF":0.7,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lernaea cf. cyprinacea infection in agile frog (Rana dalmatina) tadpoles in Hungary. 匈牙利敏捷蛙(Rana dalmatina)蝌蚪感染 Lernaea cf. cyprinacea。
IF 0.7 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2024-08-22 Print Date: 2024-09-12 DOI: 10.1556/004.2024.01046
Ádám Varga, Ferenc Zoltán Demény, András Weiperth, Árpád Ferincz, Balázs Kucska, Béla Urbányi, Tamás Müller
{"title":"Lernaea cf. cyprinacea infection in agile frog (Rana dalmatina) tadpoles in Hungary.","authors":"Ádám Varga, Ferenc Zoltán Demény, András Weiperth, Árpád Ferincz, Balázs Kucska, Béla Urbányi, Tamás Müller","doi":"10.1556/004.2024.01046","DOIUrl":"10.1556/004.2024.01046","url":null,"abstract":"<p><p>The ectoparasitic copepod Lernaea cyprinacea (anchor worm) has more than 100 host species among teleost fishes and affects cyprinids both in fish farms and natural waters. In addition, while L. cyprinacea infection in amphibians has been recorded in Asia, North and South America, there is no data available in the literature on their presence in Europe. In this study, we first reported L. cf. cyprinacea parasitising an anuran tadpole in Europe. Specimens of L. cf. cyprinacea were observed attached to a tadpole of the agile frog (Rana dalmatina), which was caught during fishing of crucian carp fingerlings (Carassius carassius) from a small fish pond in Hungary during the summer of 2012. The infected tadpole was collected from a rearing pond, where juvenile crucian carps were kept. The tadpole was inspected in the laboratory, and digital photos were taken. The parasites were found attached to the body-tail junction and to the leg of the tadpole (at Gosner stage 41). The parasite species was identified as L. cf. cyprinacea based on morphological traits.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"195-198"},"PeriodicalIF":0.7,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphometric and functional changes in the kidney of rats induced by inorganic selenium. 无机硒诱导大鼠肾脏的形态和功能变化
IF 0.7 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2024-08-13 Print Date: 2024-09-12 DOI: 10.1556/004.2024.01036
Pavle Randjelović, Niko Radulović, Nikola Stojanović, Ivan Ilić
{"title":"Morphometric and functional changes in the kidney of rats induced by inorganic selenium.","authors":"Pavle Randjelović, Niko Radulović, Nikola Stojanović, Ivan Ilić","doi":"10.1556/004.2024.01036","DOIUrl":"10.1556/004.2024.01036","url":null,"abstract":"<p><p>Selenium (Se) is an essential trace element. Depending on concentration, Se can manifest both beneficial and harmful effects on the cell and can have both oxidative and antioxidant effects. Because the therapeutic index for Se is very narrow and it can depend on the form of Se used, we aimed to investigate the potential safety and side effects of inorganic Se on kidney function and structure in rats. After 8 days of treatment, no detectable signs of microscopic changes or oxidative stress in kidney tissue were observed. We detected a moderate increase in urea and creatinine levels in the blood as a sign of a slight decrease in kidney function. The most notable changes in our study were morphometric alterations in proximal tubules and glomeruli, both of which were considerably enlarged. The short-term application of inorganic Se to rats in a supratherapeutic dose induced mild functional and ultrastructural changes in the kidneys. The dose of inorganic Se used could be considered relatively safe for short-term application in situations where Se could be beneficial to the organism, such as the usage of known nephrotoxicants or high levels of oxidative stress in the body.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"141-147"},"PeriodicalIF":0.7,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141987152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of colostrum quality using Brix refractometer in sheep. 使用 Brix 折射仪测定绵羊初乳的质量。
IF 0.7 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2024-06-27 Print Date: 2024-07-03 DOI: 10.1556/004.2024.00951
Meryem Sarica, Uğur Aydoğdu
{"title":"Determination of colostrum quality using Brix refractometer in sheep.","authors":"Meryem Sarica, Uğur Aydoğdu","doi":"10.1556/004.2024.00951","DOIUrl":"10.1556/004.2024.00951","url":null,"abstract":"<p><p>In this study, we aimed to determine the quality of colostrum in sheep by using Brix refractometer. The research included 100 sheep of Merino X Kivircik crossbred. From each, we collected 15 mL of colostrum samples in falcon tubes within the first 8 h after delivery. Mean colostral IgG level of sheep was 156.68 ± 7.23 g L-1, optical and digital Brix refractometer values (%) were determined as 27.43 ± 0.53 and 27.69 ± 0.60, respectively. Ewes carrying twin lambs produced significantly higher quality colostrum than those carrying single lambs. However, parity did not affect the colostrum quality. Optical and digital Brix values were correlated with gold standard radial immunodiffusion (RID) colostral IgG level (r = 0.70 and r = 0.64, respectively). Also, optical and digital Brix refractometers were found to be highly correlated (r = 0.98, P < 0.001). While the optimal Brix value was 22% for the 50, 60 and 70 g L-1 IgG threshold values (by means of RID as the potential good quality threshold value for ewe colostrum quality), this value was 23% for 80 g L-1. We can conclude that Brix refractometers is a valuable tool for determining ewe colostrum quality. A cut point of 22% Brix for defining good quality colostrum in ewes was most appropriate for our data.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"116-124"},"PeriodicalIF":0.7,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of retinal atrophy in mixed breed dogs using spectral domain optical coherence tomography (SD-OCT) and electroretinography. 使用光谱域光学相干断层扫描(SD-OCT)和视网膜电图评估混种犬的视网膜萎缩。
IF 0.7 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2024-06-24 Print Date: 2024-07-03 DOI: 10.1556/004.2024.00872
Ireneusz Balicki, Mateusz Szadkowski, Agnieszka Balicka, Marcin Lew, Alexandra Trbolova
{"title":"Assessment of retinal atrophy in mixed breed dogs using spectral domain optical coherence tomography (SD-OCT) and electroretinography.","authors":"Ireneusz Balicki, Mateusz Szadkowski, Agnieszka Balicka, Marcin Lew, Alexandra Trbolova","doi":"10.1556/004.2024.00872","DOIUrl":"10.1556/004.2024.00872","url":null,"abstract":"<p><p>The aim of the study was to characterize retinal atrophy (RA) with progressive retinal atrophy symptoms in mixed breed dogs using ophthalmoscopy, spectral domain optical coherence tomography (SD-OCT) and electroretinography (ERG).The study was performed on 13 mixed breed dogs affected by retinal atrophy (11 males and 2 females that were 1.5-14 years old). Depending on the advancement of RA, SD-OCT examinations identified retinal abnormalities ranging from layer disorganisation to advanced atrophy. The most advanced RA occurred ventral to the optic disc. Total retinal thickness in both eyes (mean ± SD) was lower in dogs with RA compared to controls dorsally (77.7 ± 39.5 μm vs 173.5 ± 13.3 μm), ventrally (33.4 ± 29.9 μm vs 139.5 ± 10.8 μm), nasally (65.0 ± 34.5 μm vs 163.9 ± 11.0 μm) and temporally (61.8 ± 41.7 μm vs 171.9 ± 11.1 μm) to the optic disc. In dogs with locally normal architecture of inner retina, loss of definition of outer retinal layers occurred in many regions. Dark and light-adapted ERGs were reduced in 2 dogs with RA and were unrecordable in 11 dogs. Lesions evident in SD-OCT scans of mixed breed dogs affected with retinal atrophy initially appear ventrally to the optic disc and ventro-dorsally in advanced RA. In all mixed breed dogs with retinal atrophy, clinical signs and SD-OCT results correlate with ERG findings.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"80-98"},"PeriodicalIF":0.7,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First molecular detection of Brachyspira hampsonii on pig farms in Poland. 在波兰养猪场首次分子检测到 Brachyspira hampsonii。
IF 0.7 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2024-06-19 Print Date: 2024-07-03 DOI: 10.1556/004.2024.00960
Piotr Cybulski, Katrin Strutzberg-Minder, Edyta Michalik, Radosław Kondratiuk, Artur Jabłoński
{"title":"First molecular detection of Brachyspira hampsonii on pig farms in Poland.","authors":"Piotr Cybulski, Katrin Strutzberg-Minder, Edyta Michalik, Radosław Kondratiuk, Artur Jabłoński","doi":"10.1556/004.2024.00960","DOIUrl":"10.1556/004.2024.00960","url":null,"abstract":"<p><p>Nowadays, the three strongly beta-haemolytic spirochaetes, Brachyspira hyodysenteriae, Brachyspira suanatina and Brachyspira hampsonii are thought to be causative agents of swine dysentery, an economically devastating disease of grow-finish pigs characterised by severe mucohaemorrhagic diarrhoea. B. hyodysenteriae has been reported in most leading swine-producing regions. B. suanatina and B. hampsonii have been successfully recovered from faecal samples collected in a few countries only. The present study was performed in March 2023 on faecal samples originating from nine Polish finisher farms with 6,000 to 18,000 animals in a location. Samples were obtained from 40 diarrhoeic finishers. Nucleic acid extracted from the samples was analysed using multiplex PCR for Brachyspira spp. From a total of nine sample populations examined in our study, the genetic material of B. hampsonii was identified in two. To the best of our knowledge, this is the first report on molecular detection of B. hampsonii on pig farms outside North America, Belgium and Germany. Our research highlights the need for increased focus directed on laboratory testing strategies, the lack of which may perplex swine practitioners and severely hinder a definite diagnosis.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"66-70"},"PeriodicalIF":0.7,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141426067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sero-epidemiology of bluetongue in ruminants raised on private holdings in North-Western Pakistan. 巴基斯坦西北部私人饲养的反刍动物的蓝舌病血清流行病学。
IF 0.7 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2024-06-19 Print Date: 2024-07-03 DOI: 10.1556/004.2024.01033
Muhammad Ishaq, Syed Asad Ali Shah, Midrar Ullah, Sakhawat Ali, Syed Muhammad Jamal
{"title":"Sero-epidemiology of bluetongue in ruminants raised on private holdings in North-Western Pakistan.","authors":"Muhammad Ishaq, Syed Asad Ali Shah, Midrar Ullah, Sakhawat Ali, Syed Muhammad Jamal","doi":"10.1556/004.2024.01033","DOIUrl":"10.1556/004.2024.01033","url":null,"abstract":"<p><p>This study investigated the sero-epidemiology of bluetongue in ruminants in North-Western Pakistan. A total of 3,173 serum samples were collected from small (n = 1,651) and large (n = 1,522) ruminants being reared by farmers in 14 districts. Antibodies to bluetongue virus (BTV) were detected using competitive ELISA. The overall prevalence of BTV antibodies was 65%. A significant association (P < 0.05) between the prevalence of BTV antibodies and the risk factors including sex, species, age, area, husbandry practices and breed was shown by univariate analysis. In multivariate analysis, the seroprevalence was 6.5 (95% CL = 3.7-11.4), 5.9 (95% CL = 3.8-9.4) and 2.4 (95% CL = 1.5-3.7) times higher in buffaloes, cattle and goats than sheep, respectively. The seroprevalence was 1.4 (95% CL = 1.1-1.7) times higher in local breeds than in cross/exotic breeds. The seroprevalence was 1.6 (95% CL = 1.1 to 2.3) times higher in sedentary animals than in nomadic animals. The seroprevalence was significantly associated with age. Further work is required to determine the BTV serotypes prevalent in the study area for effective control of the disease.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"133-139"},"PeriodicalIF":0.7,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141431125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Operator impact assessment on qualitative and quantitative parameters of canine platelet-rich plasma. 操作员对犬富血小板血浆定性和定量参数的影响评估。
IF 0.7 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2024-06-19 Print Date: 2024-07-03 DOI: 10.1556/004.2024.01019
S Amitha Banu, Khan Sharun, K M Manjusha, Rohit Kumar, Obli Rajendran Vinodhkumar, Ujjwal Kumar De, A M Pawde, Kuldeep Dhama
{"title":"Operator impact assessment on qualitative and quantitative parameters of canine platelet-rich plasma.","authors":"S Amitha Banu, Khan Sharun, K M Manjusha, Rohit Kumar, Obli Rajendran Vinodhkumar, Ujjwal Kumar De, A M Pawde, Kuldeep Dhama","doi":"10.1556/004.2024.01019","DOIUrl":"10.1556/004.2024.01019","url":null,"abstract":"<p><p>Platelet-rich plasma (PRP) has emerged as a cornerstone in veterinary regenerative medicine. The present study evaluated the impact of the operator on the qualitative and quantitative features of non-activated PRP derived from canine whole blood. Blood was collected in anticoagulant acid citrate dextrose from twelve healthy adult dogs and PRP was prepared according to the double-spin method. Both operators followed an identical protocol and utilized the same equipment for PRP preparation from the pooled blood samples. The resulting PRP underwent characterization, classification and coding based on minimum reporting standards. The consistency and internal reliability of different parameters were also assessed using the intraclass correlation coefficient and Cronbach's alpha values. Variables such as white blood cell (WBC) concentration, relative WBC composition and mean platelet volume (MPV) showed poor reliability, and WBC concentration and MPV also had unacceptable internal consistency. Significant differences were observed in several qualitative and quantitative parameters of the prepared PRP, highlighting the influence of the operator even when the same protocol and equipment were used. Our study has direct implications to regenerative medicine, reinforcing the urgency to set minimum requirements for reporting PRP in research studies.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"99-108"},"PeriodicalIF":0.7,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141431124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing cryopreserved ram sperm quality at -80 °C with Spirulina platensis and Salvia verbenaca extracts. 用螺旋藻和丹参提取物提高-80 °C低温保存的公羊精子质量。
IF 0.7 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2024-06-19 Print Date: 2024-07-03 DOI: 10.1556/004.2024.01042
Anass Ben Moula, Saad Salhi, Abdellatif Rahim, Mouad Chentouf, Marianne Raes, Jean Loup Bister, Bouchra El Amiri
{"title":"Enhancing cryopreserved ram sperm quality at -80 °C with Spirulina platensis and Salvia verbenaca extracts.","authors":"Anass Ben Moula, Saad Salhi, Abdellatif Rahim, Mouad Chentouf, Marianne Raes, Jean Loup Bister, Bouchra El Amiri","doi":"10.1556/004.2024.01042","DOIUrl":"10.1556/004.2024.01042","url":null,"abstract":"<p><p>This study was conducted in two steps to evaluate the influence of freezing methods and natural extracts on cryopreserved ram sperm quality. Initially, the research compared the effects of two freezing methods: liquid nitrogen (LN2) versus -80 °C, on post-thawed ram semen on total and progressive motilities and velocity parameters. Experiment I revealed no significant differences (P > 0.05) between the LN2 and -80 °C freezing methods, indicating similar effects on the analyzed parameters. Experiment II aimed to examine the influence of Spirulina platensis (SP) and Salvia verbenaca (SV) extracts added to egg yolk extender on cryopreserved sperm quality, utilizing the -80 °C freezing method. Various concentrations (1.25, 3.75, 6.25 and 8.75 µg*mL-1) of acetone (Ac-SP and Ac-SV) and hexanoic (Hex-SP), as well as methanolic (MeOH-SV) extracts, were added into the extender. A thorough assessment of post-thawed sperm quality parameters, encompassing motility, velocity parameters, viability, membrane integrity, abnormality and lipid peroxidation was conducted. The outcomes demonstrated that 1.25 and 3.75 g*mL-1 of Ac-SP and Hex-SP and 1.25 µg*mL-1 of AC-SV and MeOH-SV increased the post-thawed ram sperm quality. In conclusion, this study emphasizes the antioxidant properties of SP and SV extracts, highlighting their potential to protect cryopreserved sperm cells from oxidative stress at -80 °C.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"125-132"},"PeriodicalIF":0.7,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141431123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimetastatic effect of intratumoral Treg antagonists in mice with renal cancer. 瘤内 Treg 拮抗剂对肾癌小鼠的抗转移作用
IF 0.7 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2024-06-18 Print Date: 2024-07-03 DOI: 10.1556/004.2024.01037
Dominik Gulyás, Gábor Kovács, István Jankovics, Máté Hidvégi, Béla Dénes, Lilla Kálfalvy-Molnár, Rebeka Nagypál, Márta Lőrincz
{"title":"Antimetastatic effect of intratumoral Treg antagonists in mice with renal cancer.","authors":"Dominik Gulyás, Gábor Kovács, István Jankovics, Máté Hidvégi, Béla Dénes, Lilla Kálfalvy-Molnár, Rebeka Nagypál, Márta Lőrincz","doi":"10.1556/004.2024.01037","DOIUrl":"10.1556/004.2024.01037","url":null,"abstract":"<p><p>The interplay of regulatory T cells (Tregs) within the tumour microenvironment presents a significant challenge in anticancer immunotherapy. This study investigates the potential of Treg blockade to enhance the efficiency of effector T cells. Two distinct treatment cocktails were examined: 3p-hpRNA (5' triphosphate hairpin RNA) combined with unmethylated CpG oligonucleotide (CpG); CpG in combination with OX40 receptor-specific monoclonal antibody (anti-OX40). Treatment efficacy was assessed using a murine model of kidney adenocarcinoma.Renca cells (renal cortical cells with adenocarcinoma) were subcutaneously engrafted in 30 BALB/c mice, then animals were allocated into three treatment groups: Group 1: CpG+anti-OX40, Group 2: CpG+3p-hpRNA, Group 3: untreated control. Treatment efficacy was evaluated based on tumour growth, the occurrence of metastases and overall survival.On day 28 post-implantation, experiments had to be terminated due to tumour progression. Although comparisons of survival times and primary tumour sizes thus became inconsequential, histological examinations provided valuable insights. We observed distinct variations in primary tumour characteristics among the different groups: Groups 1 and 2 displayed demarcations, while Group 3 exhibited diffuse tumours with necrosis. Lung metastases were evident in 70% of untreated mice, whereas none were observed in either of the treated groups.Our findings instil confidence in the potential efficacy of the treatments, thereby laying a solid foundation for future investigations.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"109-115"},"PeriodicalIF":0.7,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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