{"title":"Identification of yeast in healthy and subclinical mastitis-diagnosed Anatolian buffaloes in smallholder dairy farms in Turkey.","authors":"Oguz Kagan Turedi, Esra Seker","doi":"10.1556/004.2025.01129","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to identify yeast species in healthy Anatolian buffaloes and those with subclinical mastitis on smallholder farms using the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) method and assess antifungal resistance. Milk samples from 390 udder lobes of 100 buffaloes were tested and California Mastitis Test (CMT) results showed 265 negative, 102 suspicious and 23 CMT ≥+1 lobes. A total of 76 yeast isolates (19.5%) were obtained through classical culture methods. MALDI-TOF MS identified seven genera, with the most common species being Pichia kudriavzevii (54%), Kluyveromyces marxianus (21%) and Nakaseomyces glabrata (10.5%). Antifungal resistance was determined by the disk diffusion method, showing the highest resistance to caspofungin (69.7%), followed by fluconazole (65.8%) and ketoconazole (31.6%). This study highlights the presence of diverse yeast species in buffalo milk, emphasizing the importance of understanding their role in mastitis and antifungal resistance for animal and public health. To our knowledge, this is the first study showing the identification of different yeast species isolated from Anatolian buffaloes in smallholder dairy farms in Turkey.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"104-110"},"PeriodicalIF":0.7000,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta veterinaria Hungarica","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1556/004.2025.01129","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/13 0:00:00","PubModel":"Print","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
This study aimed to identify yeast species in healthy Anatolian buffaloes and those with subclinical mastitis on smallholder farms using the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) method and assess antifungal resistance. Milk samples from 390 udder lobes of 100 buffaloes were tested and California Mastitis Test (CMT) results showed 265 negative, 102 suspicious and 23 CMT ≥+1 lobes. A total of 76 yeast isolates (19.5%) were obtained through classical culture methods. MALDI-TOF MS identified seven genera, with the most common species being Pichia kudriavzevii (54%), Kluyveromyces marxianus (21%) and Nakaseomyces glabrata (10.5%). Antifungal resistance was determined by the disk diffusion method, showing the highest resistance to caspofungin (69.7%), followed by fluconazole (65.8%) and ketoconazole (31.6%). This study highlights the presence of diverse yeast species in buffalo milk, emphasizing the importance of understanding their role in mastitis and antifungal resistance for animal and public health. To our knowledge, this is the first study showing the identification of different yeast species isolated from Anatolian buffaloes in smallholder dairy farms in Turkey.
期刊介绍:
Acta Veterinaria Hungarica publishes original research papers presenting new scientific results of international interest, and to a limited extent also review articles and clinical case reports, on veterinary physiology (physiological chemistry and metabolism), veterinary microbiology (bacteriology, virology, immunology, molecular biology), on the infectious diseases of domestic animals, on veterinary parasitology, pathology, clinical veterinary science and reproduction.