Acta veterinaria Hungarica最新文献_第10页

The impact of quercetin on a porcine intestinal epithelial cell line exposed to deoxynivalenol. 槲皮素对暴露于脱氧雪腐酚的猪肠上皮细胞系的影响。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2021-02-24 DOI: 10.1556/004.2020.00052

Judit Mercédesz Pomothy, Katrina Gatt, Ákos Jerzsele, Erzsébet Pászti Gere

{"title":"The impact of quercetin on a porcine intestinal epithelial cell line exposed to deoxynivalenol.","authors":"Judit Mercédesz Pomothy, Katrina Gatt, Ákos Jerzsele, Erzsébet Pászti Gere","doi":"10.1556/004.2020.00052","DOIUrl":"https://doi.org/10.1556/004.2020.00052","url":null,"abstract":"Quercetin (Que) is present in many vegetables and fruits as a secondary antioxidant metabolite. Deoxynivalenol (DON) produced by various Fusarium mould species can induce cytotoxicity and oxidative stress in the gastrointestinal tracts of humans and farm animals. The aim of this study was to investigate the effects of Que on DON-induced oxidative stress in a non-tumourigenic porcine IPEC-J2 cell line. Two experimental designs were used in our experiments as follows: (a) pretreatment with 20 µmol/L Que for 24 h followed by 1-h 1 µmol/L DON treatment and (b) simultaneous application of 20 µmol/L Que and 1 µmol/L DON for 1 h. Cell cytotoxicity, transepithelial electrical resistance (TER) of cell monolayers and extracellular/intracellular redox status were studied. It was found that DON significantly decreased TER and triggered oxidative stress, while Que pretreatments were beneficial in maintaining the integrity of the monolayers and alleviated oxidative stress. However, co-treatment with Que was unable to preserve the integrity and redox balance of the cells exposed to DON. These results indicate that only the 24-h preincubation of cells with 20 µmol/L Que was beneficial in compensating for the disruption caused by DON in extracellular oxidative status.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"68 4","pages":"380-386"},"PeriodicalIF":0.9,"publicationDate":"2021-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25400673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Effect of gintonin on matrix metalloproteinase-9 concentration in tears during corneal wound healing in rabbits. gintonin对兔角膜创面愈合过程中泪液中基质金属蛋白酶-9浓度的影响。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2021-02-10 DOI: 10.1556/004.2020.00062

Ha-Eun Lee, Joon-Young Kim, Soon-Wuk Jeong, Seung-Yeol Nah

{"title":"Effect of gintonin on matrix metalloproteinase-9 concentration in tears during corneal wound healing in rabbits.","authors":"Ha-Eun Lee, Joon-Young Kim, Soon-Wuk Jeong, Seung-Yeol Nah","doi":"10.1556/004.2020.00062","DOIUrl":"https://doi.org/10.1556/004.2020.00062","url":null,"abstract":"It has been shown that gintonin, isolated from Panax ginseng, can promote rapid corneal wound healing. We aimed to elucidate the underlying mechanism and investigated whether gintonin affects the concentration of the extracellular matrix remodelling factor matrix metalloproteinase-9 (MMP-9) in tears during rabbit corneal wound healing in vivo. Twelve eyes (six rabbits) were divided equally into three groups. All eyes underwent corneal de-epithelialisation. The control group received Tearin Free sodium hyaluronate 0.1%, the solcoseryl group received solcoseryl-120 concentrate, and the gintonin group received 2.5 mg gintonin in sodium hyaluronate 0.1%. All preparations were administered for 5 days and the concentration of MMP-9 was measured in tears via ELISA on days 0, 1, and 5. MMP-9 concentrations in all groups were increased at day 1 and reduced at day 5. Of note, we found a significant change over the time frame for the gintonin group (P < 0.05) but not for the control or solcoseryl groups (P > 0.05) Moreover, increased MMP-9 levels between days 0 and 1, and their reduction between days 1 and 5, were significant in the gintonin group compared to those in the other groups (P < 0.05); however, and once more, these changes were not significant between the control and solcoseryl groups (P > 0.05). In conclusion, gintonin increases the concentration of MMP-9 rapidly within a day of injury, and decreasing it thereafter.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"68 4","pages":"364-369"},"PeriodicalIF":0.9,"publicationDate":"2021-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25355631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Seroprevalence of Streptococcus equi subspecies equi in Croatia - Short communication. 克罗地亚马链球菌亚种的血清流行率-短传播。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2021-02-10 DOI: 10.1556/004.2020.00061

Zrinka Štritof, Catriona Mitchell, Nenad Turk, Josipa Habuš, Suzana Hađina, Matko Perharić, Andrew S Waller

{"title":"Seroprevalence of Streptococcus equi subspecies equi in Croatia - Short communication.","authors":"Zrinka Štritof, Catriona Mitchell, Nenad Turk, Josipa Habuš, Suzana Hađina, Matko Perharić, Andrew S Waller","doi":"10.1556/004.2020.00061","DOIUrl":"https://doi.org/10.1556/004.2020.00061","url":null,"abstract":"Clinical cases resembling strangles are regularly seen in some areas of Croatia. However, there are no data on the prevalence of infection and the clinical forms or geographic distribution of the disease. The aim of this study was to determine the seroprevalence of Streptococcus equi subspecies equi in horses resident in Croatia, in order to estimate the geographic distribution of infection. The study included 291 horse sera from the eight counties where the majority of Croatian horses are kept. Sera were tested by indirect ELISA (iELISA) for the presence of serum antibodies against S. equi protein A (SEQ_2190) and protein C (SeM). Positive horses were detected in all counties. Overall seroprevalence was 16.5 per cent (48/291), ranging from 7.1 to 29.6 per cent. A positive association was observed between the population size of the horses in the counties and the seropositivity rates: the larger the population, the higher the seropositivity. The results of this study suggest that S. equi infection is widespread in Croatia. Further investigation of the clinical manifestations, circulating strains and other characteristics of the disease in Croatia and raising awareness of the disease among horse owners are now required.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"68 4","pages":"361-363"},"PeriodicalIF":0.9,"publicationDate":"2021-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25355630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Erratum: Heart rate, heart rate variability, faecal glucocorticoid metabolites and avoidance response of dairy cows before and after changeover to an automatic milking system. 勘误:切换到自动挤奶系统前后奶牛的心率、心率变异性、粪便糖皮质激素代谢物和回避反应。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2021-02-06 DOI: 10.1556/004.2020.10000

Viktor Jurkovich, Fruzsina Luca Kézér, Ferenc Ruff, Mikolt Bakony, Margit Kulcsár, Levente Kovács

引用次数: 0

Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy. 评估两种麻醉方案对内镜下行结肠切开术的红耳滑鼠(Trachemys scripta elegans)氧化状态和DNA损伤的影响。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2021-01-27 DOI: 10.1556/004.2020.00058

Milan Došenović, Milena Radaković, Miloš Vučićević, Branislav Vejnović, Maja Vasiljević, Darko Marinković, Zoran Stanimirović

{"title":"Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy.","authors":"Milan Došenović, Milena Radaković, Miloš Vučićević, Branislav Vejnović, Maja Vasiljević, Darko Marinković, Zoran Stanimirović","doi":"10.1556/004.2020.00058","DOIUrl":"https://doi.org/10.1556/004.2020.00058","url":null,"abstract":"The aim of this study was to assess how red-eared sliders (Trachemys scripta elegans) respond to anaesthesia itself and coelioscopy. For that purpose, the turtles were anaesthetised with ketamine-medetomidine or propofol, and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and the level of malondialdehyde (MDA) were determined by spectrophotometry. The possible genotoxic effects of the anaesthetic agents were estimated by comet assay. A total of 24 turtles were included in this study. The animals were divided into four groups according to the anaesthetic protocol and according to whether endoscopy would be performed. Significantly decreased activities of CAT were found only in the propofol group and in turtles undergoing coelioscopy. Both anaesthetic protocols induced significantly increased MDA levels, while no differences were observed after the intervention. A significant increase in GST activity was detected in turtles after both anaesthetic protocols, but after coelioscopy significant changes in GST activity were found only in the propofol group. However, no differences in SOD activity and no DNA damages were detected in either group. These findings suggest that ketamine-medetomidine may be more suitable anaesthetic agents in red-eared sliders than propofol.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"68 4","pages":"337-344"},"PeriodicalIF":0.9,"publicationDate":"2021-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38790149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Biofilm-forming lactic acid bacteria of honey bee origin intended for potential probiotic use. 源自蜜蜂的生物膜形成乳酸菌，用于潜在的益生菌用途。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2021-01-25 DOI: 10.1556/004.2020.00057

Bohumil Bielik, Ladislav Molnár, Vladimír Vrabec, Romana Andrášiová, Ivana Cingel'ová Maruščáková, Radomíra Nemcová, Juraj Toporčák, Dagmar Mudroňová

{"title":"Biofilm-forming lactic acid bacteria of honey bee origin intended for potential probiotic use.","authors":"Bohumil Bielik, Ladislav Molnár, Vladimír Vrabec, Romana Andrášiová, Ivana Cingel'ová Maruščáková, Radomíra Nemcová, Juraj Toporčák, Dagmar Mudroňová","doi":"10.1556/004.2020.00057","DOIUrl":"https://doi.org/10.1556/004.2020.00057","url":null,"abstract":"Scientists around the world are focusing their interest on the use of probiotics in honey bees as an alternative method of prophylaxis against causative agents of both American and European foulbrood. In our study we tested inhibitory activity against Paenibacillus larvae and the biofilm formation activity by various lactic acid bacteria isolated from honey bee guts or fresh pollen samples in the presence of different sugars added to the cultivation media. In addition, we tested the probiotic effect of a newly selected Apilactobacillus kunkeei V18 in an in situ experiment in bee colonies. We found antibacterial activity against P. larvae in four isolates. Biofilm formation activity of varying intensity was noted in six of the seven isolates in the presence of different sugars. The strongest biofilm formation (OD570 ≥ 1) was noted in A. kunkeei V18 in the presence of fructose; moreover, this isolate strongly inhibited the growth of P. larvae under laboratory conditions. Inhibition of P. larvae and Melissococcus plutonius by A. kunkeei V18 in situ was confirmed in a pilot study.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"68 4","pages":"345-353"},"PeriodicalIF":0.9,"publicationDate":"2021-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38860611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Percutaneous aspiration-injection-reaspiration (PAIR) for the treatment of abdominal cysts: Initial report in sheep and goat. 经皮吸入-注射-呼吸(PAIR)治疗腹部囊肿:绵羊和山羊的初步报告。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2021-01-16 DOI: 10.1556/004.2020.00059

Nida Handoo, Mujeeb Ur Rehman Fazili, Mohammad Abrar Gayas, Hakim Athar, Raja Tariq

{"title":"Percutaneous aspiration-injection-reaspiration (PAIR) for the treatment of abdominal cysts: Initial report in sheep and goat.","authors":"Nida Handoo, Mujeeb Ur Rehman Fazili, Mohammad Abrar Gayas, Hakim Athar, Raja Tariq","doi":"10.1556/004.2020.00059","DOIUrl":"https://doi.org/10.1556/004.2020.00059","url":null,"abstract":"Percutaneous aspiration-injection-reaspiration (PAIR), also called sclerotherapy, is a minimally invasive, inexpensive and safe technique for the treatment of abdominal cysts in humans. A study was planned to evaluate the feasibility of this procedure in the management of abdominal cysts in sheep and goat. Adult ewes (n = 5) and one doe (n = 1) found to have abdominal cysts (one cyst/animal) on repeated survey ultrasonography (USG) were included in the study. The animals were restrained in standing position. A hypodermic needle (G-18) securely attached to a 10-mL Dispovan syringe was carefully passed under ultrasound guidance into the abdominal cyst in all these animals. Depending on the size of the cyst, 1.0-5.5 mL fluid was aspirated, and 0.5-2.0 mL of 20% hypertonic saline solution infused. The needle was thereafter kept in situ for 10 min. The maximum possible volume of the cyst content was reaspirated and the needle withdrawn. On day 7, sclerotherapy was repeated in five animals showing no appreciable reduction in cyst size by USG. USG was repeated on days 30 and 90. All the cysts except one responded to PAIR during this period. From this study it can be concluded that sclerotherapy using hypertonic saline (20%) is a minimally invasive, inexpensive, effective and safe interventional ultrasonographic technique for the treatment of abdominal cysts in sheep and goats. However, the procedure needs further evaluation after using different sclerotic agents of varying concentrations and duration of their retention in the cysts in a sufficient number of animals with cysts.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"68 4","pages":"393-398"},"PeriodicalIF":0.9,"publicationDate":"2021-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38830146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Lithium chloride outperformed oxalic acid sublimation in a preliminary experiment for Varroa mite control in pre-wintering honey bee colonies. 氯化锂对越冬前蜂群的螨虫防治效果优于草酸升华法。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2021-01-16 DOI: 10.1556/004.2020.00060

Éva Kolics, András Specziár, János Taller, Kinga Klára Mátyás, Balázs Kolics

引用次数: 10

Prevalence of feline leukaemia virus and feline immunodeficiency virus in domestic cats in Ireland. 猫白血病病毒和猫免疫缺陷病毒在爱尔兰家猫中的流行。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2021-01-16 DOI: 10.1556/004.2020.00056

Anna Szilasi, Lilla Dénes, Eszter Krikó, Caoimhe Murray, Míra Mándoki, Gyula Balka

引用次数: 5

Experimental evaluation of cortical screw placement in lag fashion into the distal phalanx in standing horses. 直立马远端指骨内皮质螺钉滞后置入的实验评价。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2021-01-15 DOI: 10.1556/004.2020.00054

Simon Izing, Dániel Béni, Szabina Molnár, Zoltán Bakos, Gábor Bodó

{"title":"Experimental evaluation of cortical screw placement in lag fashion into the distal phalanx in standing horses.","authors":"Simon Izing, Dániel Béni, Szabina Molnár, Zoltán Bakos, Gábor Bodó","doi":"10.1556/004.2020.00054","DOIUrl":"https://doi.org/10.1556/004.2020.00054","url":null,"abstract":"The objectives of this in vivo experimental study were to evaluate the feasibility of cortical screw insertion into the intact distal phalanx in standing sedated horses and to document potential postoperative complications. One cortical screw was randomly inserted in lag fashion into each distal phalanx in 9 horses. The second surgery on the contralateral limbs was performed 2-3 weeks after the first operation, when a 4.5-mm cortical screw was inserted in lag fashion into the distal phalanx of sedated horses following perineural analgesia. Following surgery, the drill hole was filled with an antibiotic-soaked swab, which was changed every 48 h. The horses were euthanised 8 weeks after the second surgery. The hooves were disarticulated and evaluated macroscopically and by computed tomography. The surgery time was 13.9 ± 4.8 min (mean ± SD). Pain scores and lameness gradually decreased after 7 days. Solar canal penetration (SCP) was detected in 10 out of the 18 distal phalanges (55.5%). In 7 out of the 10 penetrations intraoperative bleeding was obvious. No postoperative infection was observed. Screw insertion into the distal phalanx was easily and quickly accomplished in standing horses, but its advantages in horses with sagittal fractures should be investigated further. SCP had no impact on postoperative lameness.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"68 4","pages":"399-404"},"PeriodicalIF":0.9,"publicationDate":"2021-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38830149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0