Acta veterinaria Hungarica最新文献_第3页

First molecular detection of Brachyspira hampsonii on pig farms in Poland. 在波兰养猪场首次分子检测到 Brachyspira hampsonii。

IF 0.7 4区农林科学

Acta veterinaria Hungarica Pub Date : 2024-06-19 Print Date: 2024-07-03 DOI: 10.1556/004.2024.00960

Piotr Cybulski, Katrin Strutzberg-Minder, Edyta Michalik, Radosław Kondratiuk, Artur Jabłoński

{"title":"First molecular detection of Brachyspira hampsonii on pig farms in Poland.","authors":"Piotr Cybulski, Katrin Strutzberg-Minder, Edyta Michalik, Radosław Kondratiuk, Artur Jabłoński","doi":"10.1556/004.2024.00960","DOIUrl":"10.1556/004.2024.00960","url":null,"abstract":"Nowadays, the three strongly beta-haemolytic spirochaetes, Brachyspira hyodysenteriae, Brachyspira suanatina and Brachyspira hampsonii are thought to be causative agents of swine dysentery, an economically devastating disease of grow-finish pigs characterised by severe mucohaemorrhagic diarrhoea. B. hyodysenteriae has been reported in most leading swine-producing regions. B. suanatina and B. hampsonii have been successfully recovered from faecal samples collected in a few countries only. The present study was performed in March 2023 on faecal samples originating from nine Polish finisher farms with 6,000 to 18,000 animals in a location. Samples were obtained from 40 diarrhoeic finishers. Nucleic acid extracted from the samples was analysed using multiplex PCR for Brachyspira spp. From a total of nine sample populations examined in our study, the genetic material of B. hampsonii was identified in two. To the best of our knowledge, this is the first report on molecular detection of B. hampsonii on pig farms outside North America, Belgium and Germany. Our research highlights the need for increased focus directed on laboratory testing strategies, the lack of which may perplex swine practitioners and severely hinder a definite diagnosis.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"66-70"},"PeriodicalIF":0.7,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141426067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sero-epidemiology of bluetongue in ruminants raised on private holdings in North-Western Pakistan. 巴基斯坦西北部私人饲养的反刍动物的蓝舌病血清流行病学。

IF 0.7 4区农林科学

Acta veterinaria Hungarica Pub Date : 2024-06-19 Print Date: 2024-07-03 DOI: 10.1556/004.2024.01033

Muhammad Ishaq, Syed Asad Ali Shah, Midrar Ullah, Sakhawat Ali, Syed Muhammad Jamal

{"title":"Sero-epidemiology of bluetongue in ruminants raised on private holdings in North-Western Pakistan.","authors":"Muhammad Ishaq, Syed Asad Ali Shah, Midrar Ullah, Sakhawat Ali, Syed Muhammad Jamal","doi":"10.1556/004.2024.01033","DOIUrl":"10.1556/004.2024.01033","url":null,"abstract":"This study investigated the sero-epidemiology of bluetongue in ruminants in North-Western Pakistan. A total of 3,173 serum samples were collected from small (n = 1,651) and large (n = 1,522) ruminants being reared by farmers in 14 districts. Antibodies to bluetongue virus (BTV) were detected using competitive ELISA. The overall prevalence of BTV antibodies was 65%. A significant association (P < 0.05) between the prevalence of BTV antibodies and the risk factors including sex, species, age, area, husbandry practices and breed was shown by univariate analysis. In multivariate analysis, the seroprevalence was 6.5 (95% CL = 3.7-11.4), 5.9 (95% CL = 3.8-9.4) and 2.4 (95% CL = 1.5-3.7) times higher in buffaloes, cattle and goats than sheep, respectively. The seroprevalence was 1.4 (95% CL = 1.1-1.7) times higher in local breeds than in cross/exotic breeds. The seroprevalence was 1.6 (95% CL = 1.1 to 2.3) times higher in sedentary animals than in nomadic animals. The seroprevalence was significantly associated with age. Further work is required to determine the BTV serotypes prevalent in the study area for effective control of the disease.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"133-139"},"PeriodicalIF":0.7,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141431125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Operator impact assessment on qualitative and quantitative parameters of canine platelet-rich plasma. 操作员对犬富血小板血浆定性和定量参数的影响评估。

IF 0.7 4区农林科学

Acta veterinaria Hungarica Pub Date : 2024-06-19 Print Date: 2024-07-03 DOI: 10.1556/004.2024.01019

S Amitha Banu, Khan Sharun, K M Manjusha, Rohit Kumar, Obli Rajendran Vinodhkumar, Ujjwal Kumar De, A M Pawde, Kuldeep Dhama

{"title":"Operator impact assessment on qualitative and quantitative parameters of canine platelet-rich plasma.","authors":"S Amitha Banu, Khan Sharun, K M Manjusha, Rohit Kumar, Obli Rajendran Vinodhkumar, Ujjwal Kumar De, A M Pawde, Kuldeep Dhama","doi":"10.1556/004.2024.01019","DOIUrl":"10.1556/004.2024.01019","url":null,"abstract":"Platelet-rich plasma (PRP) has emerged as a cornerstone in veterinary regenerative medicine. The present study evaluated the impact of the operator on the qualitative and quantitative features of non-activated PRP derived from canine whole blood. Blood was collected in anticoagulant acid citrate dextrose from twelve healthy adult dogs and PRP was prepared according to the double-spin method. Both operators followed an identical protocol and utilized the same equipment for PRP preparation from the pooled blood samples. The resulting PRP underwent characterization, classification and coding based on minimum reporting standards. The consistency and internal reliability of different parameters were also assessed using the intraclass correlation coefficient and Cronbach's alpha values. Variables such as white blood cell (WBC) concentration, relative WBC composition and mean platelet volume (MPV) showed poor reliability, and WBC concentration and MPV also had unacceptable internal consistency. Significant differences were observed in several qualitative and quantitative parameters of the prepared PRP, highlighting the influence of the operator even when the same protocol and equipment were used. Our study has direct implications to regenerative medicine, reinforcing the urgency to set minimum requirements for reporting PRP in research studies.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"99-108"},"PeriodicalIF":0.7,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141431124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing cryopreserved ram sperm quality at -80 °C with Spirulina platensis and Salvia verbenaca extracts. 用螺旋藻和丹参提取物提高-80 °C低温保存的公羊精子质量。

IF 0.7 4区农林科学

Acta veterinaria Hungarica Pub Date : 2024-06-19 Print Date: 2024-07-03 DOI: 10.1556/004.2024.01042

Anass Ben Moula, Saad Salhi, Abdellatif Rahim, Mouad Chentouf, Marianne Raes, Jean Loup Bister, Bouchra El Amiri

{"title":"Enhancing cryopreserved ram sperm quality at -80 °C with Spirulina platensis and Salvia verbenaca extracts.","authors":"Anass Ben Moula, Saad Salhi, Abdellatif Rahim, Mouad Chentouf, Marianne Raes, Jean Loup Bister, Bouchra El Amiri","doi":"10.1556/004.2024.01042","DOIUrl":"10.1556/004.2024.01042","url":null,"abstract":"This study was conducted in two steps to evaluate the influence of freezing methods and natural extracts on cryopreserved ram sperm quality. Initially, the research compared the effects of two freezing methods: liquid nitrogen (LN2) versus -80 °C, on post-thawed ram semen on total and progressive motilities and velocity parameters. Experiment I revealed no significant differences (P > 0.05) between the LN2 and -80 °C freezing methods, indicating similar effects on the analyzed parameters. Experiment II aimed to examine the influence of Spirulina platensis (SP) and Salvia verbenaca (SV) extracts added to egg yolk extender on cryopreserved sperm quality, utilizing the -80 °C freezing method. Various concentrations (1.25, 3.75, 6.25 and 8.75 µg*mL-1) of acetone (Ac-SP and Ac-SV) and hexanoic (Hex-SP), as well as methanolic (MeOH-SV) extracts, were added into the extender. A thorough assessment of post-thawed sperm quality parameters, encompassing motility, velocity parameters, viability, membrane integrity, abnormality and lipid peroxidation was conducted. The outcomes demonstrated that 1.25 and 3.75 g*mL-1 of Ac-SP and Hex-SP and 1.25 µg*mL-1 of AC-SV and MeOH-SV increased the post-thawed ram sperm quality. In conclusion, this study emphasizes the antioxidant properties of SP and SV extracts, highlighting their potential to protect cryopreserved sperm cells from oxidative stress at -80 °C.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"125-132"},"PeriodicalIF":0.7,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141431123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimetastatic effect of intratumoral Treg antagonists in mice with renal cancer. 瘤内 Treg 拮抗剂对肾癌小鼠的抗转移作用

IF 0.7 4区农林科学

Acta veterinaria Hungarica Pub Date : 2024-06-18 Print Date: 2024-07-03 DOI: 10.1556/004.2024.01037

Dominik Gulyás, Gábor Kovács, István Jankovics, Máté Hidvégi, Béla Dénes, Lilla Kálfalvy-Molnár, Rebeka Nagypál, Márta Lőrincz

{"title":"Antimetastatic effect of intratumoral Treg antagonists in mice with renal cancer.","authors":"Dominik Gulyás, Gábor Kovács, István Jankovics, Máté Hidvégi, Béla Dénes, Lilla Kálfalvy-Molnár, Rebeka Nagypál, Márta Lőrincz","doi":"10.1556/004.2024.01037","DOIUrl":"10.1556/004.2024.01037","url":null,"abstract":"The interplay of regulatory T cells (Tregs) within the tumour microenvironment presents a significant challenge in anticancer immunotherapy. This study investigates the potential of Treg blockade to enhance the efficiency of effector T cells. Two distinct treatment cocktails were examined: 3p-hpRNA (5' triphosphate hairpin RNA) combined with unmethylated CpG oligonucleotide (CpG); CpG in combination with OX40 receptor-specific monoclonal antibody (anti-OX40). Treatment efficacy was assessed using a murine model of kidney adenocarcinoma.Renca cells (renal cortical cells with adenocarcinoma) were subcutaneously engrafted in 30 BALB/c mice, then animals were allocated into three treatment groups: Group 1: CpG+anti-OX40, Group 2: CpG+3p-hpRNA, Group 3: untreated control. Treatment efficacy was evaluated based on tumour growth, the occurrence of metastases and overall survival.On day 28 post-implantation, experiments had to be terminated due to tumour progression. Although comparisons of survival times and primary tumour sizes thus became inconsequential, histological examinations provided valuable insights. We observed distinct variations in primary tumour characteristics among the different groups: Groups 1 and 2 displayed demarcations, while Group 3 exhibited diffuse tumours with necrosis. Lung metastases were evident in 70% of untreated mice, whereas none were observed in either of the treated groups.Our findings instil confidence in the potential efficacy of the treatments, thereby laying a solid foundation for future investigations.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"109-115"},"PeriodicalIF":0.7,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thoughts on the entanglement of electromagnetism and life: A theoretical study. 关于电磁与生命纠缠的思考：理论研究。

IF 0.7 4区农林科学

Acta veterinaria Hungarica Pub Date : 2024-06-18 Print Date: 2024-07-03 DOI: 10.1556/004.2023.00976

Attila Zsarnovszky

引用次数: 0

Antimicrobial susceptibility of Pasteurella multocida strains isolated from different host species. 从不同宿主物种中分离出的多杀性巴氏杆菌菌株的抗菌药敏感性。

IF 0.7 4区农林科学

Acta veterinaria Hungarica Pub Date : 2024-06-05 Print Date: 2024-07-03 DOI: 10.1556/004.2024.01051

Krisztina Pintér, Boglárka Dóra Pollák, Hubert Gantelet, Tibor Magyar

引用次数: 0

Curcumin mitigates ochratoxin A-induced oxidative stress and alters gene expression in broiler chicken liver and kidney. 姜黄素能减轻赭曲霉毒素 A 诱导的氧化应激，并改变肉鸡肝脏和肾脏中的基因表达。

IF 0.7 4区农林科学

Acta veterinaria Hungarica Pub Date : 2024-03-26 Print Date: 2024-04-12 DOI: 10.1556/004.2024.01016

Benjámin Kövesi, Adanu Paul Worlanyo, Szabina Kulcsár, Zsolt Ancsin, Márta Erdélyi, Erika Zándoki, Miklós Mézes, Krisztián Balogh

{"title":"Curcumin mitigates ochratoxin A-induced oxidative stress and alters gene expression in broiler chicken liver and kidney.","authors":"Benjámin Kövesi, Adanu Paul Worlanyo, Szabina Kulcsár, Zsolt Ancsin, Márta Erdélyi, Erika Zándoki, Miklós Mézes, Krisztián Balogh","doi":"10.1556/004.2024.01016","DOIUrl":"10.1556/004.2024.01016","url":null,"abstract":"The study aimed to evaluate the effect of curcumin (CURC) supplementation on broiler chickens exposed to ochratoxin A (OTA), by examining biochemical parameters and the expression of glutathione redox system genes and their regulation. OTA reduced glutathione content in the liver while increasing glutathione peroxidase activity. CURC showed no significant effects. Kidney parameters remained mostly unaffected. Gene expression analysis revealed OTA-induced upregulation of KEAP1, NRF2, AHR, GPx4 and GSR genes in the liver. CURC supplementation led to the upregulation of GPx4 and AHR genes with OTA+CURC treatment, resulting in the downregulation of GPx4, KEAP1, NRF2 and AHR genes compared to OTA treatment alone. In the kidney, GPx4 was downregulated, and NRF2 and AHR were upregulated as an effect of OTA, while CURC upregulated the NRF2 gene only. OTA+CURC treatment led to the downregulation of GPx4, GSS and AHR genes compared to the control and downregulation of NRF2 and AHR genes compared to OTA. The results suggested that CURC is partly effective against OTA-induced oxidative stress and that the effect of OTA and CURC on the antioxidant response is regulated through the KEAP1-NRF2-ARE and AHR pathways.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"41-50"},"PeriodicalIF":0.7,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140304362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cumulative effect of arsenic on the number of mouse offspring and the female reproductive hormones in mice. 砷对小鼠后代数量和雌性生殖激素的累积效应。

IF 0.7 4区农林科学

Acta veterinaria Hungarica Pub Date : 2024-03-25 Print Date: 2024-04-12 DOI: 10.1556/004.2023.00965

Anita Birinji, Kristina Pogrmić-Majkić, Željko Mihaljev, Marija Marin, Dušan Lalošević

{"title":"Cumulative effect of arsenic on the number of mouse offspring and the female reproductive hormones in mice.","authors":"Anita Birinji, Kristina Pogrmić-Majkić, Željko Mihaljev, Marija Marin, Dušan Lalošević","doi":"10.1556/004.2023.00965","DOIUrl":"10.1556/004.2023.00965","url":null,"abstract":"In this study, we evaluated the cumulative effects of arsenic (III) oxide on the number of mouse offspring over three consecutive generations and monitored changes in levels of the reproductive hormones, oestradiol and progesterone in female mice during the dioestrus phase of the cycle. The control group received water from the mains. In two experimental groups, mice were given drinking water containing dissolved arsenic (III) oxide at concentrations of 10.6 mg L-1 and 106 mg L-1, respectively. These concentrations represent the values converted from a human model to an animal model (mice) thus correspond to the arsenic content of the groundwater in the southern part of the Pannonian Basin, in the province of Vojvodina, in the Banat region, in particular in the town of Zrenjanin. The average number of newborn mice in both experimental groups decreased for three consecutive generations. The total arsenic content of day-old mice did not show significant differences between the experimental groups. Arsenic (III) oxide affected the reproductive hormone levels of female mice at both concentrations.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":"33-40"},"PeriodicalIF":0.7,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140292446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro efficacy testing of ivermectin against Tritrichomonas foetus trophozoites. 伊维菌素对胎盘滋养体的体外药效测试

IF 0.7 4区农林科学

Acta veterinaria Hungarica Pub Date : 2024-02-15 Print Date: 2024-04-12 DOI: 10.1556/004.2024.01023

Estefanía Sereno-Bruno, Lumila Fuchs, Jorge Oyhenart

引用次数: 0