BMC biomedical engineering最新文献

{"title":"3D-printed microplate inserts for long term high-resolution imaging of live brain organoids.","authors":"Mariana Oksdath Mansilla, Camilo Salazar-Hernandez, Sally L Perrin, Kaitlin G Scheer, Gökhan Cildir, John Toubia, Kristyna Sedivakova, Melinda N Tea, Sakthi Lenin, Elise Ponthier, Erica C F Yeo, Vinay Tergaonkar, Santosh Poonnoose, Rebecca J Ormsby, Stuart M Pitson, Michael P Brown, Lisa M Ebert, Guillermo A Gomez","doi":"10.1186/s42490-021-00049-5","DOIUrl":"10.1186/s42490-021-00049-5","url":null,"abstract":"<p><strong>Background: </strong>Organoids are a reliable model used in the study of human brain development and under pathological conditions. However, current methods for brain organoid culture generate tissues that range from 0.5 to 2 mm of size, which need to be constantly agitated to allow proper oxygenation. The culture conditions are, therefore, not suitable for whole-brain organoid live imaging, required to study developmental processes and disease progression within physiologically relevant time frames (i.e. days, weeks, months).</p><p><strong>Results: </strong>Here we designed 3D-printed microplate inserts adaptable to standard 24 multi-well plates, which allow the growth of multiple organoids in pre-defined and fixed XYZ coordinates. This innovation facilitates high-resolution imaging of whole-cerebral organoids, allowing precise assessment of organoid growth and morphology, as well as cell tracking within the organoids, over long periods. We applied this technology to track neocortex development through neuronal progenitors in brain organoids, as well as the movement of patient-derived glioblastoma stem cells within healthy brain organoids.</p><p><strong>Conclusions: </strong>This new bioengineering platform constitutes a significant advance that permits long term detailed analysis of whole-brain organoids using multimodal inverted fluorescence microscopy.</p>","PeriodicalId":72425,"journal":{"name":"BMC biomedical engineering","volume":"3 1","pages":"6"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25550161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A carbon nanotubes based in situ multifunctional power assist system for restoring failed heart function.","authors":"Quanfu Xu, Yuli Yang, Jianwen Hou, Taizhong Chen, Yudong Fei, Qian Wang, Qing Zhou, Wei Li, Jing Ren, Yi-Gang Li","doi":"10.1186/s42490-021-00051-x","DOIUrl":"10.1186/s42490-021-00051-x","url":null,"abstract":"<p><strong>Background: </strong>End-stage heart failure is a major risk of mortality. The conductive super-aligned carbon nanotubes sheets (SA-CNTs) has been applied to restore the structure and function of injured myocardium through tissue engineering, and developed as efficient cardiac pacing electrodes. However, the interfacial interaction between SA-CNTs and the surface cells is unclear, and it remains challenge to restore the diminished contraction for a seriously damaged heart.</p><p><strong>Results: </strong>A concept of a multifunctional power assist system (MPS) capable of multipoint pacing and contraction assisting is proposed. This device is designed to work with the host heart and does not contact blood, thus avoiding long-term anticoagulation required in current therapies. Pacing electrode constructed by SA--CNTs promotes the epithelial-mesenchymal transition and directs the migration of pro-regenerative epicardial cells. Meanwhile, the power assist unit reveals an excellent frequency response to alternating voltage, with natural heart mimicked systolic/diastolic amplitudes. Moreover, this system exhibits an excellent pacing when attached to the surface of a rabbit heart, and presents nice biocompatibility in both in vitro and in vivo evaluation.</p><p><strong>Conclusions: </strong>This MPS provides a promising non-blood contact strategy to restore in situ the normal blood-pumping function of a failed heart.</p>","PeriodicalId":72425,"journal":{"name":"BMC biomedical engineering","volume":"3 1","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2021-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995575/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25521094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modified U-Net for liver cancer segmentation from computed tomography images with a new class balancing method.","authors":"Yodit Abebe Ayalew, Kinde Anlay Fante, Mohammed Aliy Mohammed","doi":"10.1186/s42490-021-00050-y","DOIUrl":"10.1186/s42490-021-00050-y","url":null,"abstract":"<p><strong>Background: </strong>Liver cancer is the sixth most common cancer worldwide. It is mostly diagnosed with a computed tomography scan. Nowadays deep learning methods have been used for the segmentation of the liver and its tumor from the computed tomography (CT) scan images. This research mainly focused on segmenting liver and tumor from the abdominal CT scan images using a deep learning method and minimizing the effort and time used for a liver cancer diagnosis. The algorithm is based on the original UNet architecture. But, here in this paper, the numbers of filters on each convolutional block were reduced and new batch normalization and a dropout layer were added after each convolutional block of the contracting path.</p><p><strong>Results: </strong>Using this algorithm a dice score of 0.96, 0.74, and 0.63 were obtained for liver segmentation, segmentation of tumors from the liver, and the segmentation of tumor from abdominal CT scan images respectively. The segmentation results of liver and tumor from the liver showed an improvement of 0.01 and 0.11 respectively from other works.</p><p><strong>Conclusion: </strong>This work proposed a liver and a tumor segmentation method using a UNet architecture as a baseline. Modification regarding the number of filters and network layers were done on the original UNet model to reduce the network complexity and improve segmentation performance. A new class balancing method is also introduced to minimize the class imbalance problem. Through these, the algorithm attained better segmentation results and showed good improvement. However, it faced difficulty in segmenting small and irregular tumors.</p>","PeriodicalId":72425,"journal":{"name":"BMC biomedical engineering","volume":"3 1","pages":"4"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25412355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Approaches in cooling of resistive coil-based low-field Magnetic Resonance Imaging (MRI) systems for application in low resource settings.","authors":"Faith Natukunda,&nbsp;Theodora M Twongyirwe,&nbsp;Steven J Schiff,&nbsp;Johnes Obungoloch","doi":"10.1186/s42490-021-00048-6","DOIUrl":"https://doi.org/10.1186/s42490-021-00048-6","url":null,"abstract":"<p><p>Magnetic Resonance Imaging (MRI), a non-invasive method for the diagnosis of diverse health conditions has experienced growing popularity over other imaging modalities like ultrasound and Computer Tomography. Initially, proof-of-concept and earlier MRI systems were based on resistive and permanent magnet technology. However, superconducting magnets have long held monopoly of the market for MRI systems with their high-field (HF) strength capability, although they present high construction, installation, and siting requirements. Such stringent prerequisites restrict their availability and use in low-middle income countries. Resistive coil-based magnet, albeit low-field (LF) in capacity, represent a plausible boost for the availability and use of MRI systems in resource constrained settings. These systems are characterized by low costs coupled with substantial image quality for diagnosis of some conditions such as hydrocephalus common is such regions. However, the nature of resistive coils causes them to heat up during operation, thus necessitating a dedicated cooling system to improve image quality and enhance system longevity. This paper explores a range of cooling methods as have been applied to resistive magnets, citing their pros and cons and areas for improvement.</p>","PeriodicalId":72425,"journal":{"name":"BMC biomedical engineering","volume":"3 1","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2021-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42490-021-00048-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25363804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantifying the variability between multiple multiplanar reconstructions of computed tomography scans.","authors":"James E Miles,&nbsp;Lene E Buelund","doi":"10.1186/s42490-021-00047-7","DOIUrl":"https://doi.org/10.1186/s42490-021-00047-7","url":null,"abstract":"<p><strong>Background: </strong>Multiplanar reconstructions of computed tomography (CT) scans can alleviate issues with bone or joint positioning during scan acquisition. The repeatability of these reconstructions is dependent on human operators applying reconstruction criteria, and therefore is subject to error, which could affect measurement reliability for angular or spatial measurements made for orthopaedic surgery. We describe a method for quantifying inter-reconstruction variability numerically and graphically using metadata from the CT header to find vectors describing reconstruction axis alignment. The approach is demonstrated using 3 sets of computed tomographic reconstructions of 24 vulpine femorotibial joints.</p><p><strong>Results: </strong>Vectors describing axis alignments permitted identification and subsequent analysis of deviations from optimal alignment between reconstruction sets. For the worked example, alignment deviations equivalent to femoral abduction/adduction were nearly twice those for extension/flexion, and simulation of the effects of these deviations on measurements closely matched published data.</p><p><strong>Conclusions: </strong>The method presented here is straightforward and permits numerical and graphical analysis of reconstruction variability. Reconstruction alignment variability should be considered before adopting new reconstruction criteria for clinical use, and evaluated whenever there is suspicion that reconstruction variability could unduly influence subsequent measurements. These evaluations may help drive improvements in reconstruction criteria. The methods described here could also be employed for comparing patient positioning between scans and between different scan modalities.</p>","PeriodicalId":72425,"journal":{"name":"BMC biomedical engineering","volume":"3 1","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42490-021-00047-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25317565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electric field stimulation for tissue engineering applications.","authors":"Christina N M Ryan,&nbsp;Meletios N Doulgkeroglou,&nbsp;Dimitrios I Zeugolis","doi":"10.1186/s42490-020-00046-0","DOIUrl":"https://doi.org/10.1186/s42490-020-00046-0","url":null,"abstract":"<p><p>Electric fields are involved in numerous physiological processes, including directional embryonic development and wound healing following injury. To study these processes in vitro and/or to harness electric field stimulation as a biophysical environmental cue for organised tissue engineering strategies various electric field stimulation systems have been developed. These systems are overall similar in design and have been shown to influence morphology, orientation, migration and phenotype of several different cell types. This review discusses different electric field stimulation setups and their effect on cell response.</p>","PeriodicalId":72425,"journal":{"name":"BMC biomedical engineering","volume":"3 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2021-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42490-020-00046-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38780755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel suprachoroidal microinvasive glaucoma implant: in vivo biocompatibility and biointegration.","authors":"Ian Grierson,&nbsp;Don Minckler,&nbsp;Marian K Rippy,&nbsp;Andrew J Marshall,&nbsp;Nathalie Collignon,&nbsp;Jessica Bianco,&nbsp;Benoit Detry,&nbsp;Murray A Johnstone","doi":"10.1186/s42490-020-00045-1","DOIUrl":"https://doi.org/10.1186/s42490-020-00045-1","url":null,"abstract":"<p><strong>Background: </strong>A major challenge for any glaucoma implant is their ability to provide long-term intraocular pressure lowering efficacy. The formation of a low-permeability fibrous capsule around the device often leads to obstructed drainage channels, which may impair the drainage function of devices. These foreign body-related limitations point to the need to develop biologically inert biomaterials to improve performance in reaching long-term intraocular pressure reduction. The aim of this study was to evaluate in vivo (in rabbits) the ocular biocompatibility and tissue integration of a novel suprachoroidal microinvasive glaucoma implant, MINIject™ (iSTAR Medical, Wavre, Belgium).</p><p><strong>Results: </strong>In two rabbit studies, no biocompatibility issue was induced by the suprachoroidal, ab-externo implantation of the MINIject™ device. Clinical evaluation throughout the 6 post-operative months between the sham and test groups were similar, suggesting most reactions were related to the ab-externo surgical technique used for rabbits, rather than the implant material itself. Histological analysis of ocular tissues at post-operative months 1, 3 and 6 revealed that the implant was well-tolerated and induced only minimal fibroplasia and thus minimal encapsulation around the implant. The microporous structure of the device became rapidly colonized by cells, mostly by macrophages through cell migration, which do not, by their nature, impede the flow of aqueous humor through the device. Time-course analysis showed that once established, pore colonization was stable over time. No fibrosis nor dense connective tissue development were observed within any implant at any time point. The presence of pore colonization may be the process by which encapsulation around the implant is minimized, thus preserving the permeability of the surrounding tissues. No degradation nor structural changes of the implant occurred during the course of both studies.</p><p><strong>Conclusions: </strong>The novel MINIject™ microinvasive glaucoma implant was well-tolerated in ocular tissues of rabbits, with observance of biointegration, and no biocompatibility issues. Minimal fibrous encapsulation and stable cellular pore colonization provided evidence of preserved drainage properties over time, suggesting that the implant may produce a long-term ability to enhance aqueous outflow.</p>","PeriodicalId":72425,"journal":{"name":"BMC biomedical engineering","volume":"2 ","pages":"10"},"PeriodicalIF":0.0,"publicationDate":"2020-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42490-020-00045-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38605838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bismuth nanoparticles obtained by a facile synthesis method exhibit antimicrobial activity against <i>Staphylococcus aureus</i> and <i>Candida albicans</i>.","authors":"Roberto Vazquez-Munoz,&nbsp;M Josefina Arellano-Jimenez,&nbsp;Jose L Lopez-Ribot","doi":"10.1186/s42490-020-00044-2","DOIUrl":"https://doi.org/10.1186/s42490-020-00044-2","url":null,"abstract":"<p><strong>Background: </strong>Bismuth compounds are known for their activity against multiple microorganisms; yet, the antibiotic properties of bismuth nanoparticles (BiNPs) remain poorly explored. The objective of this work is to further the research of BiNPs for nanomedicine-related applications. Stable Polyvinylpyrrolidone (PVP)-coated BiNPs were produced by a chemical reduction process, in less than 30 min.</p><p><strong>Results: </strong>We produced stable, small, spheroid PVP-coated BiNPs with a crystalline organization. The PVP-BiNPs showed potent antibacterial activity against the pathogenic bacterium <i>Staphylococcus aureus</i> and antifungal activity against the opportunistic pathogenic yeast <i>Candida albicans</i>, both under planktonic and biofilm growing conditions.</p><p><strong>Conclusions: </strong>Our results indicate that BiNPs represent promising antimicrobial nanomaterials, and this facile synthetic method may allow for further investigation of their activity against a variety of pathogenic microorganisms.</p>","PeriodicalId":72425,"journal":{"name":"BMC biomedical engineering","volume":"2 ","pages":"11"},"PeriodicalIF":0.0,"publicationDate":"2020-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42490-020-00044-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38605839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An open-source phase correction toolkit for transcranial focused ultrasound.","authors":"Changzhu Jin, David Moore, John Snell, Dong-Guk Paeng","doi":"10.1186/s42490-020-00043-3","DOIUrl":"10.1186/s42490-020-00043-3","url":null,"abstract":"<p><strong>Background: </strong>The phase correction on transcranial focused ultrasound is essential to regulate unwanted focal point shift caused by skull bone aberration. The aim of the current study was to design and investigate the feasibility of a ray-based phase correction toolkit for transcranial focused ultrasound.</p><p><strong>Results: </strong>The peak pressure at focal area was improved by 140.5 ± 7.0% on target I and 134.8 ± 19.1% on target II using proposed phase correction toolkit, respectively. A total computation time of 402.1 ± 24.5 milliseconds was achieved for each sonication.</p><p><strong>Conclusion: </strong>The designed ray-based phase correction software can be used as a lightweight toolkit to compensate aberrated phase within clinical environment.</p>","PeriodicalId":72425,"journal":{"name":"BMC biomedical engineering","volume":"2 ","pages":"9"},"PeriodicalIF":0.0,"publicationDate":"2020-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7427913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38358689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Workplace activity classification from shoe-based movement sensors.","authors":"Jonatan Fridolfsson,&nbsp;Daniel Arvidsson,&nbsp;Frithjof Doerks,&nbsp;Theresa J Kreidler,&nbsp;Stefan Grau","doi":"10.1186/s42490-020-00042-4","DOIUrl":"https://doi.org/10.1186/s42490-020-00042-4","url":null,"abstract":"<p><strong>Background: </strong>High occupational physical activity is associated with lower health. Shoe-based movement sensors can provide an objective measurement of occupational physical activity in a lab setting but the performance of such methods in a free-living environment have not been investigated. The aim of the current study was to investigate the feasibility and accuracy of shoe sensor-based activity classification in an industrial work setting.</p><p><strong>Results: </strong>An initial calibration part was performed with 35 subjects who performed different workplace activities in a structured lab setting while the movement was measured by a shoe-sensor. Three different machine-learning models (random forest (RF), support vector machine and k-nearest neighbour) were trained to classify activities using the collected lab data. In a second validation part, 29 industry workers were followed at work while an observer noted their activities and the movement was captured with a shoe-based movement sensor. The performance of the trained classification models were validated using the free-living workplace data. The RF classifier consistently outperformed the other models with a substantial difference in in the free-living validation. The accuracy of the initial RF classifier was 83% in the lab setting and 43% in the free-living validation. After combining activities that was difficult to discriminate the accuracy increased to 96 and 71% in the lab and free-living setting respectively. In the free-living part, 99% of the collected samples either consisted of stationary activities or walking.</p><p><strong>Conclusions: </strong>Walking and stationary activities can be classified with high accuracy from a shoe-based movement sensor in a free-living occupational setting. The distribution of activities at the workplace should be considered when validating activity classification models in a free-living setting.</p>","PeriodicalId":72425,"journal":{"name":"BMC biomedical engineering","volume":"2 ","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2020-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42490-020-00042-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38454819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}