Biomaterials and biosystems最新文献

{"title":"Optimization and evaluation of oxygen-plasma-modified, aligned, poly (Є-caprolactone) and silk fibroin nanofibrous scaffold for corneal stromal regeneration","authors":"Promita Bhattacharjee ,&nbsp;Peter W. Madden ,&nbsp;Enzo Patriarca ,&nbsp;Mark Ahearne","doi":"10.1016/j.bbiosy.2023.100083","DOIUrl":"10.1016/j.bbiosy.2023.100083","url":null,"abstract":"<div><p>The shortage of human donor corneas for transplantation necessitates the exploration of tissue engineering approaches to develop corneal substitutes. However, these substitutes must possess the necessary strength, transparency, and ability to regulate cell behaviour before they can be used in patients. In this study, we investigated the effectiveness of an oxygen plasma surface-modified poly-ε-caprolactone (PCL) combined with silk fibroin (SF) nanofibrous scaffold for corneal stromal regeneration. To fabricate the electrospun scaffolds, PCL and SF blends were used on a rotating mandrel. The optimization of the blend aimed to replicate the structural and functional properties of the human cornea, focusing on nanofibre alignment, mechanical characteristics, and <em>in vitro</em> cytocompatibility with human corneal stromal keratocytes. Surface modification of the scaffold resulted in improved transparency and enhanced cell interaction. Based on the evaluation, a composite nanofibrous scaffold with a 1:1 blend of PCL and SF was selected for a more comprehensive analysis. The biological response of keratocytes to the scaffold was assessed through cellular adhesion, proliferation, cytoskeletal organization, gene expression, and immunocytochemical staining. The scaffold facilitated the adhesion of corneal stromal cells, supporting cell proliferation, maintaining normal cytoskeletal organization, and promoting increased expression of genes associated with healthy corneal stromal keratocytes. These findings highlight the potential of a surface-modified PCL/SF blend (1:1) as a promising scaffolding material for corneal stromal regeneration. The developed scaffold not only demonstrated favourable biological interactions with corneal stromal cells but also exhibited characteristics aligned with the requirements for successful corneal tissue engineering. Further research and refinement of these constructs could lead to significant advancements in addressing the shortage of corneas for transplantation, ultimately improving the treatment outcomes for patients in need.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"12 ","pages":"Article 100083"},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9c/cc/main.PMC10507194.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41158841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal stromal cell exosomes enhance dental pulp cell functions and promote pulp-dentin regeneration","authors":"Jiajun Shi ,&nbsp;Kristeen Ye Wen Teo ,&nbsp;Shipin Zhang ,&nbsp;Ruenn Chai Lai ,&nbsp;Vinicius Rosa ,&nbsp;Huei Jinn Tong ,&nbsp;Mandeep S. Duggal ,&nbsp;Sai Kiang Lim ,&nbsp;Wei Seong Toh","doi":"10.1016/j.bbiosy.2023.100078","DOIUrl":"10.1016/j.bbiosy.2023.100078","url":null,"abstract":"<div><p>Mesenchymal stromal/stem cell (MSC) therapies are currently being explored for dental pulp regeneration. As the therapeutic effects of MSCs in tissue repair are mediated mainly through the release of extracellular vesicles (EVs) including exosomes, we investigated here the cellular processes and molecular mechanisms modulated by MSC exosomes in dental pulp regeneration. Using dental pulp cell (DPC) cultures, we demonstrated that MSC exosomes could increase DPC migration, proliferation, and odontogenic differentiation. The enhancement of these cellular processes was mediated through exosomal CD73-mediated adenosine receptor activation of AKT and ERK signaling. Consistent with these observations, MSC exosomes increased the expression of dentin matrix proteins and promoted the formation of dentin-like tissue and bridge-like structures in a rat pulp defect model. These effects were comparable to that of mineral trioxide aggregate (MTA) treatment. MSC exosomes also yielded recellularized pulp-dentin tissues in the root canal of endodontically-treated human premolars, following subcutaneous implantation in the mouse dorsum. Together, our findings suggest that MSC exosomes could exert a multi-faceted effect on DPC functions including migration, proliferation and odontogenic differentiation to promote dental pulp regeneration. This study provides the basis for development of MSC exosomes as a cell-free MSC therapeutic alternative for pulp-dentin regeneration.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"11 ","pages":"Article 100078"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/33/ef/main.PMC10239699.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9592197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical cues are not potent regulators of human dermal fibroblast trans-differentiation","authors":"Christina N.M. Ryan ,&nbsp;Eugenia Pugliese ,&nbsp;Naledi Shologu ,&nbsp;Diana Gaspar ,&nbsp;Peadar Rooney ,&nbsp;Md Nahidul Islam ,&nbsp;Alan O'Riordan ,&nbsp;Manus J. Biggs ,&nbsp;Matthew D. Griffin ,&nbsp;Dimitrios I. Zeugolis","doi":"10.1016/j.bbiosy.2023.100079","DOIUrl":"10.1016/j.bbiosy.2023.100079","url":null,"abstract":"<div><p>Due to their inherent plasticity, dermal fibroblasts hold great promise in regenerative medicine. Although biological signals have been well-established as potent regulators of dermal fibroblast function, it is still unclear whether physiochemical cues can induce dermal fibroblast trans-differentiation. Herein, we evaluated the combined effect of surface topography, substrate rigidity, collagen type I coating and macromolecular crowding in human dermal fibroblast cultures. Our data indicate that tissue culture plastic and collagen type I coating increased cell proliferation and metabolic activity. None of the assessed in vitro microenvironment modulators affected cell viability. Anisotropic surface topography induced bidirectional cell morphology, especially on more rigid (1,000 kPa and 130 kPa) substrates. Macromolecular crowding increased various collagen types, but not fibronectin, deposition. Macromolecular crowding induced globular extracellular matrix deposition, independently of the properties of the substrate. At day 14 (longest time point assessed), macromolecular crowding downregulated tenascin C (in 9 out of the 14 groups), aggrecan (in 13 out of the 14 groups), osteonectin (in 13 out of the 14 groups), and collagen type I (in all groups). Overall, our data suggest that physicochemical cues (such surface topography, substrate rigidity, collagen coating and macromolecular crowding) are not as potent as biological signals in inducing dermal fibroblast trans-differentiation.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"11 ","pages":"Article 100079"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10499661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10289871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomaterials are the key to unlock spheroid function and therapeutic potential","authors":"David H. Ramos-Rodriguez ,&nbsp;J. Kent Leach","doi":"10.1016/j.bbiosy.2023.100080","DOIUrl":"10.1016/j.bbiosy.2023.100080","url":null,"abstract":"<div><p>Spheroids are three-dimensional cell aggregates that mimic fundamental aspects of the native tissue microenvironment better than single cells, making them a promising platform for the study of tissue development and therapeutics. Spheroids have been investigated for decades as models in cancer research, yet we have only just scratched the surface of their potential clinical utility in cell-based therapies. Like many cells, spheroids commonly exhibit a loss of key attributes upon implantation, motivating the need for strategies to regulate their function <em>in situ</em>. Biomaterials offer numerous opportunities to preserve spheroid function and guide spheroid behavior by tailoring the local microenvironment.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"11 ","pages":"Article 100080"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10499638/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10290855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun decellularized extracellular matrix scaffolds promote the regeneration of injured neurons","authors":"Lena Mungenast ,&nbsp;Ronya Nieminen ,&nbsp;Carine Gaiser ,&nbsp;Ana Bela Faia-Torres ,&nbsp;Jürgen Rühe ,&nbsp;Laura Suter-Dick","doi":"10.1016/j.bbiosy.2023.100081","DOIUrl":"10.1016/j.bbiosy.2023.100081","url":null,"abstract":"<div><p>Traumatic injury to the spinal cord (SCI) causes the transection of neurons, formation of a lesion cavity, and remodeling of the microenvironment by excessive extracellular matrix (ECM) deposition and scar formation leading to a regeneration-prohibiting environment. Electrospun fiber scaffolds have been shown to simulate the ECM and increase neural alignment and neurite outgrowth contributing to a growth-permissive matrix. In this work, electrospun ECM-like fibers providing biochemical and topological cues are implemented into a scaffold to represent an oriented biomaterial suitable for the alignment and migration of neural cells in order to improve spinal cord regeneration. The successfully decellularized spinal cord ECM (dECM), with no visible cell nuclei and dsDNA content &lt; 50 ng/mg tissue, showed preserved ECM components, such as glycosaminoglycans and collagens. Serving as the biomaterial for 3D printer-assisted electrospinning, highly aligned and randomly distributed dECM fiber scaffolds (&lt; 1 µm fiber diameter) were fabricated. The scaffolds were cytocompatible and supported the viability of a human neural cell line (SH-SY5Y) for 14 days. Cells were selectively differentiated into neurons, as confirmed by immunolabeling of specific cell markers (ChAT, Tubulin ß), and followed the orientation given by the dECM scaffolds. After generating a lesion site on the cell-scaffold model, cell migration was observed and compared to reference poly-ε-caprolactone fiber scaffolds. The aligned dECM fiber scaffold promoted the fastest and most efficient lesion closure, indicating superior cell guiding capabilities of dECM-based scaffolds. The strategy of combining decellularized tissues with controlled deposition of fibers to optimize biochemical and topographical cues opens the way for clinically relevant central nervous system scaffolding solutions.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"11 ","pages":"Article 100081"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9e/8e/main.PMC10329103.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9811693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disruptive 3D in vitro models for respiratory disease investigation: A state-of-the-art approach focused on SARS-CoV-2 infection","authors":"Maria Luiza Seixas ,&nbsp;Cynthia Silva Bartolomeo ,&nbsp;Robertha Lemes ,&nbsp;Tiago Nicoliche ,&nbsp;Liria Hiromi Okuda ,&nbsp;Leonardo Martins ,&nbsp;Rodrigo Ureshino ,&nbsp;Carla Maximo Prado ,&nbsp;Tácia Tavares Aquinas Liguori ,&nbsp;Gabriel Romero Liguori ,&nbsp;Roberta Sessa Stilhano","doi":"10.1016/j.bbiosy.2023.100082","DOIUrl":"10.1016/j.bbiosy.2023.100082","url":null,"abstract":"<div><p>COVID-19, along with most respiratory diseases in the medical field, demonstrates significant ability to take its toll on global population. There is a particular difficulty in studying these conditions, which stems especially from the short supply of <em>in vitro</em> models for detailed investigation, the specific therapeutic knowledge required for disease scrutinization and the occasional need of BSL-3 [Biosafety Level 3] laboratories for research. Based on this, the process of drug development is hampered to a great extent. In the scenario of COVID-19, this difficulty is even more substantial on account of the current undefinition regarding the exact role of the ACE2 [Angiotensin-converting enzyme 2] receptor upon SARS-CoV-2 kinetics in human cells and the great level of demand in the investigation process of ACE2, which usually requires the laborious and ethically complicated usage of transgenic animal models overexpressing the receptor. Moreover, the rapid progression of the aforementioned diseases, especially COVID-19, poses a crucial necessity for adequate therapeutic solutions emergence. In this context, the work herein presented introduces a groundbreaking set of 3D models, namely spheroids and MatriWell cell culture inserts, whose remarkable ability to mimic the in vivo environment makes them highly suitable for respiratory diseases investigation, particularly SARS-CoV-2 infection. Using MatriWells, we developed an innovative platform for COVID-19 research: a pulmonary air-liquid interface [ALI] associated with endothelial (HUVEC) cells. Infection studies revealed that pulmonary (BEAS-2B) cells in the ALI reached peak viral load at 24h and endothelial cells, at 48h, demonstrating lung viral replication and subsequent hematogenous dissemination, which provides us with a unique and realistic framework for studying COVID-19. Simultaneously, the spheroids were used to address the understudied ACE2 receptor, aiming at a pronounced process of COVID-19 investigation. ACE2 expression not only increased spheroid diameter by 20% (p&lt;0.001) and volume by 60% (p≤0.0001) but also led to a remarkable 640-fold increase in intracellular viral load (p≤0.01). The previously mentioned finding supports ACE2 as a potential target for COVID-19 treatment. Lastly, we observed a higher viral load in the MatriWells compared to spheroids (150-fold, p&lt;0.0001), suggesting the MatriWells as a more appropriate approach for COVID-19 investigation. By establishing an advanced method for respiratory tract conditions research, this work paves the way toward an efficacious process of drug development, contributing to a change in the course of respiratory diseases such as COVID-19.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"11 ","pages":"Article 100082"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e9/93/main.PMC10391659.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10308365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical and in vitro biological behaviors of a Ti-Mo-Fe alloy specifically designed for stent applications","authors":"Carolina Catanio Bortolan ,&nbsp;Francesco Copes ,&nbsp;Masoud Shekargoftar ,&nbsp;Vinicius de Oliveira Fidelis Sales ,&nbsp;Carlo Paternoster ,&nbsp;Leonardo Contri Campanelli ,&nbsp;Nicolas Giguère ,&nbsp;Diego Mantovani","doi":"10.1016/j.bbiosy.2023.100076","DOIUrl":"10.1016/j.bbiosy.2023.100076","url":null,"abstract":"<div><p>There is a deep interest in developing new Ni-free Ti-based alloys to replace 316 L stainless steel and Co-Cr alloys for endovascular stent application, mainly because the release of Ni can generate toxicity and allergenicity. Interactions of Ti alloy biomaterials with bone cells and tissues have been widely investigated and reported, while interactions with vascular cells and tissues, such as endothelial cells (ECs) and smooth muscle cells (SMCs), are scarce. Therefore, this study focused on the relationship among the surface finishing features, corrosion behavior and in vitro biological performances regarding human ECs, SMCs and blood of a newly developed Ti-8Mo-2Fe (TMF) alloy, specifically designed for balloon-expandable stent applications. The alloy performances were compared to those of 316 L and pure Ti, prepared with the same surface finishing techniques, which are mechanical polishing and electropolishing. Surface properties were investigated by scanning electron microscopy (SEM), atomic force microscopy (AFM), contact angle (CA) and x-ray photoelectron spectroscopy (XPS). The corrosion behavior was assessed with potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) tests in phosphate buffered saline (PBS) solution. No significant differences were observed regarding the corrosion rate measured with PDP analyses, which was of the order of 2 × 10⁻⁴ mm/y for all the studied materials. Moreover, similarly to pure Ti, TMF exhibited an advantage over 316 L for biomedical applications, namely remarkable resistance to pitting corrosion up to high potentials. The results evidenced a good cytocompatibility and hemocompatibility, making this group of alloy a potential candidate for cardiovascular implants. In fact, both ECs and SMCs proliferated on TMF surfaces showing a 7-day viability similar to that of pure Ti. Regarding hemocompatibility, TMF did not cause hemolysis, and blood coagulation was delayed on its surface in comparison to pure Ti. When compared to 316 L, TMF showed similar hemocompatibility.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"10 ","pages":"Article 100076"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/02/8f/main.PMC10240522.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9591920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The matrix reloaded – Addressing structural integrity of the aortic wall in aneurysmal disease","authors":"Jessica E. Wagenseil","doi":"10.1016/j.bbiosy.2023.100072","DOIUrl":"10.1016/j.bbiosy.2023.100072","url":null,"abstract":"<div><p>Thoracic aortic aneurysms and dissections (TAADs) involve dilation of the aortic wall that can lead to tearing or rupture. Progressive extracellular matrix (ECM) degradation is common in TAAD, regardless of the underlying cause. TAAD treatments typically target cellular signaling pathways, rather than the ECM itself, due to the complex assembly process and long half-life of ECM proteins. Compounds that stabilize the ECM are proposed as an alternative TAAD therapy that addresses the underlying cause of aortic wall failure, namely compromised structural integrity. Compounds are discussed that revisit historical approaches to maintain and preserve structural integrity of biological tissues.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"9 ","pages":"Article 100072"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c8/34/main.PMC10036219.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9188399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory nanosystems: An emerging strategy to combat viral infections","authors":"Sajmina Khatun,&nbsp;Chandra Lekha Putta,&nbsp;Arshadul Hak,&nbsp;Aravind Kumar Rengan","doi":"10.1016/j.bbiosy.2023.100073","DOIUrl":"10.1016/j.bbiosy.2023.100073","url":null,"abstract":"<div><p>The viral infection spreads with the assistance of a host. Traditional antiviral therapies cannot provide long-term immunity against emerging and drug-resistant viral infections. Immunotherapy has evolved as an efficient approach for disease prevention and treatment, which include cancer, infections, inflammatory, and immune disorders. Immunomodulatory nanosystems can dramatically enhance therapeutic outcomes by combating many therapeutic challenges, such as poor immune stimulation and off-target adverse effects. Recently, immunomodulatory nanosystems have emerged as a potent antiviral strategy to intercept viral infections effectively. This review introduces major viral infections with their primary symptoms, route of transmission &amp; targeted organ, and different stages of the viral life cycle with respective traditional blockers. The IMNs have an exceptional capacity for precisely modulating the immune system for therapeutic applications. The nano sized immunomodulatory systems permit the immune cells to interact with infectious agents enhancing lymphatic drainage and endocytosis by the over-reactive immune cells in the infected areas. Immune cells that can be modulated upon viral infection via various immunomodulatory nanosystems have been discussed. Advancement in theranostics can yield an accurate diagnosis, adequate treatment, and real-time screening of viral infections. Nanosystem-based drug delivery can continue to thrive in diagnosing, treating, and preventing viral infections. The curative medicine for remerging and drug-resistant viruses remains challenging, though certain systems have expanded our perception and initiated a new research domain in antiviral treatments.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"9 ","pages":"Article 100073"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0e/a7/main.PMC10036237.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9188400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decellularization compromises mechanical and structural properties of the native trachea","authors":"Allison M. Greaney ,&nbsp;Abhay B. Ramachandra ,&nbsp;Yifan Yuan ,&nbsp;Arina Korneva ,&nbsp;Jay D. Humphrey ,&nbsp;Laura E. Niklason","doi":"10.1016/j.bbiosy.2023.100074","DOIUrl":"10.1016/j.bbiosy.2023.100074","url":null,"abstract":"<div><p>Tracheal replacement using tissue engineering technologies offers great potential to improve previously intractable clinical interventions, and interest in this area has increased in recent years. Many engineered airway constructs currently rely on decellularized native tracheas to serve as the scaffold for tissue repair. Yet, mechanical failure leading to airway narrowing and collapse remains a major cause of morbidity and mortality following clinical implantation of decellularized tracheal grafts. To understand better the factors contributing to mechanical failure <em>in vivo</em>, we characterized the histo-mechanical properties of tracheas following two different decellularization protocols, including one that has been used clinically. All decellularized tracheas deviated from native mechanical behavior, which may provide insights into observed <em>in vivo</em> graft failures. We further analyzed protein content by western blot and analyzed microstructure by histological staining and found that the specific method of decellularization resulted in significant differences in the depletion of proteoglycans and degradation of collagens I, II, III, and elastin. Taken together, this work demonstrates that the heterogeneous architecture and mechanical behavior of the trachea is severely compromised by decellularization. Such structural deterioration may contribute to graft failure clinically and limit the potential of decellularized native tracheas as viable long-term orthotopic airway replacements.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"9 ","pages":"Article 100074"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/77/e5/main.PMC10036236.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9188401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}