Biomaterials and biosystems最新文献

{"title":"ECM biomaterials for modeling of outflow cell biology in health and disease","authors":"Souvik Ghosh ,&nbsp;Samuel Herberg","doi":"10.1016/j.bbiosy.2024.100091","DOIUrl":"https://doi.org/10.1016/j.bbiosy.2024.100091","url":null,"abstract":"<div><p>This review highlights the importance of extracellular matrix (ECM) biomaterials in understanding the biology of human trabecular meshwork (TM) and Schlemm's canal (SC) cells under normal and simulated glaucoma-like conditions. We provide an overview of recent progress in the development and application of state-of-the-art 3D ECM biomaterials including cell-derived ECM, ECM scaffolds, Matrigel, and ECM hydrogels for studies of TM and SC cell (patho)biology. Such bioengineered platforms enable accurate and reliable modeling of tissue-like cell-cell and cell-ECM interactions. They bridge the gap between conventional 2D approaches and <em>in vivo</em>/<em>ex vivo</em> models, and have the potential to aid in the identification of the causal mechanism(s) for outflow dysfunction in ocular hypertensive glaucoma. We discuss each model's benefits and limitations, and close with an outlook on future directions.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"13 ","pages":"Article 100091"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666534424000047/pdfft?md5=261f90dfd08029e29063bff5029d8bdc&pid=1-s2.0-S2666534424000047-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140030929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tribological loading of cartilage and chondrogenic cells","authors":"Yann D Ladner,&nbsp;Martin J. Stoddart","doi":"10.1016/j.bbiosy.2024.100088","DOIUrl":"https://doi.org/10.1016/j.bbiosy.2024.100088","url":null,"abstract":"<div><p>Novel cartilage regeneration therapies often look promising <em>in-vitro</em> but fail when implanted <em>in vivo.</em> One of the possible reasons for this discrepancy is the simplified, static <em>in-vitro</em> chondrogenesis models typically used. Complex mechanical stimulation plays a key role in physiological cartilage and chondrogenic cell metabolism, including the development of cartilage structure, yet it is routinely lacking during <em>in-vitro</em> studies. Multiaxial load bioreactors are becoming more widespread and offer advantages over more simple loading devices. Within this article, we highlight some of the important findings from <em>in-vitro</em> assays and key aspects relating to tribological loading of cartilage and chondrogenic cells.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"13 ","pages":"Article 100088"},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666534424000023/pdfft?md5=922f992e1af45e86fbcd854c7a5bc649&pid=1-s2.0-S2666534424000023-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139744174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printed hybrid scaffolds do not induce adverse inflammation in mice and direct human BM-MSC chondrogenesis in vitro","authors":"Silvia A. Ferreira ,&nbsp;Francesca Tallia ,&nbsp;Agathe Heyraud ,&nbsp;Simone A. Walker ,&nbsp;Christoph Salzlechner ,&nbsp;Julian R. Jones ,&nbsp;Sara M. Rankin","doi":"10.1016/j.bbiosy.2024.100087","DOIUrl":"10.1016/j.bbiosy.2024.100087","url":null,"abstract":"<div><p>Biomaterials that can improve the healing of articular cartilage lesions are needed. To address this unmet need, we developed novel 3D printed silica/poly(tetrahydrofuran)/poly(ε-caprolactone) (SiO₂/PTHF/PCL-diCOOH) hybrid scaffolds. Our aim was to carry out essential studies to advance this medical device towards functional validation in pre-clinical trials. First, we show that the chemical composition, microarchitecture and mechanical properties of these scaffolds were not affected by sterilisation with gamma irradiation. To evaluate the systemic and local immunogenic reactivity of the sterilised 3D printed hybrid scaffolds, they were implanted subcutaneously into Balb/c mice. The scaffolds did not trigger a systemic inflammatory response over one week of implantation. The interaction between the host immune system and the implanted scaffold elicited a local physiological reaction with infiltration of mononuclear cells without any signs of a chronic inflammatory response.</p><p>Then, we investigated how these 3D printed hybrid scaffolds direct chondrogenesis <em>in vitro</em>. Human bone marrow-derived mesenchymal stem/stromal cells (hBM-MSCs) seeded within the 3D printed hybrid scaffolds were cultured under normoxic or hypoxic conditions, with or without chondrogenic supplements. Chondrogenic differentiation assessed by both gene expression and protein production analyses showed that 3D printed hybrid scaffolds support hBM-MSC chondrogenesis<em>.</em> Articular cartilage-specific extracellular matrix deposition within these scaffolds was enhanced under hypoxic conditions (1.7 or 3.7 fold increase in the median of aggrecan production in basal or chondrogenic differentiation media).</p><p>Our findings show that 3D printed SiO₂/PTHF/PCL-diCOOH hybrid scaffolds have the potential to support the regeneration of cartilage tissue.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"13 ","pages":"Article 100087"},"PeriodicalIF":0.0,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666534424000011/pdfft?md5=b41b005e94cd429e5efe1d7a4e7ffc1c&pid=1-s2.0-S2666534424000011-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139455737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printed bioabsorbable composite scaffolds of poly (lactic acid)-tricalcium phosphate-ceria with osteogenic property for bone regeneration","authors":"Samarah V. Harb ,&nbsp;Elayaraja Kolanthai ,&nbsp;Abinaya S. Pugazhendhi ,&nbsp;Cesar A.G. Beatrice ,&nbsp;Leonardo A. Pinto ,&nbsp;Craig J. Neal ,&nbsp;Eduardo H. Backes ,&nbsp;Ana C.C. Nunes ,&nbsp;Heloisa S. Selistre-de-Araújo ,&nbsp;Lidiane C. Costa ,&nbsp;Melanie J. Coathup ,&nbsp;Sudipta Seal ,&nbsp;Luiz A. Pessan","doi":"10.1016/j.bbiosy.2023.100086","DOIUrl":"https://doi.org/10.1016/j.bbiosy.2023.100086","url":null,"abstract":"<div><p>The fabrication of customized implants by additive manufacturing has allowed continued development of the personalized medicine field. Herein, a 3D-printed bioabsorbable poly (lactic acid) (PLA)- β-tricalcium phosphate (TCP) (10 wt %) composite has been modified with CeO₂ nanoparticles (CeNPs) (1, 5 and 10 wt %) for bone repair. The filaments were prepared by melt extrusion and used to print porous scaffolds. The nanocomposite scaffolds possessed precise structure with fine print resolution, a homogenous distribution of TCP and CeNP components, and mechanical properties appropriate for bone tissue engineering applications. Cell proliferation assays using osteoblast cultures confirmed the cytocompatibility of the composites. In addition, the presence of CeNPs enhanced the proliferation and differentiation of mesenchymal stem cells; thereby, increasing alkaline phosphatase (ALP) activity, calcium deposition and bone-related gene expression. Results from this study have shown that the 3D printed PLA-TCP-10%CeO₂ composite scaffold could be used as an alternative polymeric implant for bone tissue engineering applications: avoiding additional/revision surgeries and accelerating the regenerative process.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"13 ","pages":"Article 100086"},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666534423000156/pdfft?md5=9dcdfda4ed3614a4d05a8b4573afea9a&pid=1-s2.0-S2666534423000156-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138839709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rise of tissue- and species-specific 3D bioprinting based on decellularized extracellular matrix-derived bioinks and bioresins","authors":"Laura Elomaa ,&nbsp;Ahed Almalla ,&nbsp;Eriselda Keshi ,&nbsp;Karl H. Hillebrandt ,&nbsp;Igor M. Sauer ,&nbsp;Marie Weinhart","doi":"10.1016/j.bbiosy.2023.100084","DOIUrl":"https://doi.org/10.1016/j.bbiosy.2023.100084","url":null,"abstract":"<div><p>Thanks to its natural complexity and functionality, decellularized extracellular matrix (dECM) serves as an excellent foundation for creating highly cell-compatible bioinks and bioresins. This enables the bioprinted cells to thrive in an environment that closely mimics their native ECM composition and offers customizable biomechanical properties. To formulate dECM bioinks and bioresins, one must first pulverize and/or solubilize the dECM into non-crosslinked fragments, which can then be chemically modified as needed. In bioprinting, the solubilized dECM-derived material is typically deposited and/or crosslinked in a layer-by-layer fashion to build 3D hydrogel structures. Since the introduction of the first liver-derived dECM-based bioinks, a wide variety of decellularized tissue have been employed in bioprinting, including kidney, heart, cartilage, and adipose tissue among others. This review aims to summarize the critical steps involved in tissue-derived dECM bioprinting, starting from the decellularization of the ECM to the standardized formulation of bioinks and bioresins, ultimately leading to the reproducible bioprinting of tissue constructs. Notably, this discussion also covers photocrosslinkable dECM bioresins, which are particularly attractive due to their ability to provide precise spatiotemporal control over the gelation in bioprinting. Both in extrusion printing and vat photopolymerization, there is a need for more standardized protocols to fully harness the unique properties of dECM-derived materials. In addition to mammalian tissues, the most recent bioprinting approaches involve the use of microbial extracellular polymeric substances in bioprinting of bacteria. This presents similar challenges as those encountered in mammalian cell printing and represents a fascinating frontier in bioprinting technology.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"12 ","pages":"Article 100084"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666534423000132/pdfft?md5=53266b4687d1013a16fc02ee5ddb1502&pid=1-s2.0-S2666534423000132-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134655545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization and evaluation of oxygen-plasma-modified, aligned, poly (Є-caprolactone) and silk fibroin nanofibrous scaffold for corneal stromal regeneration","authors":"Promita Bhattacharjee ,&nbsp;Peter W. Madden ,&nbsp;Enzo Patriarca ,&nbsp;Mark Ahearne","doi":"10.1016/j.bbiosy.2023.100083","DOIUrl":"10.1016/j.bbiosy.2023.100083","url":null,"abstract":"<div><p>The shortage of human donor corneas for transplantation necessitates the exploration of tissue engineering approaches to develop corneal substitutes. However, these substitutes must possess the necessary strength, transparency, and ability to regulate cell behaviour before they can be used in patients. In this study, we investigated the effectiveness of an oxygen plasma surface-modified poly-ε-caprolactone (PCL) combined with silk fibroin (SF) nanofibrous scaffold for corneal stromal regeneration. To fabricate the electrospun scaffolds, PCL and SF blends were used on a rotating mandrel. The optimization of the blend aimed to replicate the structural and functional properties of the human cornea, focusing on nanofibre alignment, mechanical characteristics, and <em>in vitro</em> cytocompatibility with human corneal stromal keratocytes. Surface modification of the scaffold resulted in improved transparency and enhanced cell interaction. Based on the evaluation, a composite nanofibrous scaffold with a 1:1 blend of PCL and SF was selected for a more comprehensive analysis. The biological response of keratocytes to the scaffold was assessed through cellular adhesion, proliferation, cytoskeletal organization, gene expression, and immunocytochemical staining. The scaffold facilitated the adhesion of corneal stromal cells, supporting cell proliferation, maintaining normal cytoskeletal organization, and promoting increased expression of genes associated with healthy corneal stromal keratocytes. These findings highlight the potential of a surface-modified PCL/SF blend (1:1) as a promising scaffolding material for corneal stromal regeneration. The developed scaffold not only demonstrated favourable biological interactions with corneal stromal cells but also exhibited characteristics aligned with the requirements for successful corneal tissue engineering. Further research and refinement of these constructs could lead to significant advancements in addressing the shortage of corneas for transplantation, ultimately improving the treatment outcomes for patients in need.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"12 ","pages":"Article 100083"},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9c/cc/main.PMC10507194.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41158841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal stromal cell exosomes enhance dental pulp cell functions and promote pulp-dentin regeneration","authors":"Jiajun Shi ,&nbsp;Kristeen Ye Wen Teo ,&nbsp;Shipin Zhang ,&nbsp;Ruenn Chai Lai ,&nbsp;Vinicius Rosa ,&nbsp;Huei Jinn Tong ,&nbsp;Mandeep S. Duggal ,&nbsp;Sai Kiang Lim ,&nbsp;Wei Seong Toh","doi":"10.1016/j.bbiosy.2023.100078","DOIUrl":"10.1016/j.bbiosy.2023.100078","url":null,"abstract":"<div><p>Mesenchymal stromal/stem cell (MSC) therapies are currently being explored for dental pulp regeneration. As the therapeutic effects of MSCs in tissue repair are mediated mainly through the release of extracellular vesicles (EVs) including exosomes, we investigated here the cellular processes and molecular mechanisms modulated by MSC exosomes in dental pulp regeneration. Using dental pulp cell (DPC) cultures, we demonstrated that MSC exosomes could increase DPC migration, proliferation, and odontogenic differentiation. The enhancement of these cellular processes was mediated through exosomal CD73-mediated adenosine receptor activation of AKT and ERK signaling. Consistent with these observations, MSC exosomes increased the expression of dentin matrix proteins and promoted the formation of dentin-like tissue and bridge-like structures in a rat pulp defect model. These effects were comparable to that of mineral trioxide aggregate (MTA) treatment. MSC exosomes also yielded recellularized pulp-dentin tissues in the root canal of endodontically-treated human premolars, following subcutaneous implantation in the mouse dorsum. Together, our findings suggest that MSC exosomes could exert a multi-faceted effect on DPC functions including migration, proliferation and odontogenic differentiation to promote dental pulp regeneration. This study provides the basis for development of MSC exosomes as a cell-free MSC therapeutic alternative for pulp-dentin regeneration.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"11 ","pages":"Article 100078"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/33/ef/main.PMC10239699.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9592197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical cues are not potent regulators of human dermal fibroblast trans-differentiation","authors":"Christina N.M. Ryan ,&nbsp;Eugenia Pugliese ,&nbsp;Naledi Shologu ,&nbsp;Diana Gaspar ,&nbsp;Peadar Rooney ,&nbsp;Md Nahidul Islam ,&nbsp;Alan O'Riordan ,&nbsp;Manus J. Biggs ,&nbsp;Matthew D. Griffin ,&nbsp;Dimitrios I. Zeugolis","doi":"10.1016/j.bbiosy.2023.100079","DOIUrl":"10.1016/j.bbiosy.2023.100079","url":null,"abstract":"<div><p>Due to their inherent plasticity, dermal fibroblasts hold great promise in regenerative medicine. Although biological signals have been well-established as potent regulators of dermal fibroblast function, it is still unclear whether physiochemical cues can induce dermal fibroblast trans-differentiation. Herein, we evaluated the combined effect of surface topography, substrate rigidity, collagen type I coating and macromolecular crowding in human dermal fibroblast cultures. Our data indicate that tissue culture plastic and collagen type I coating increased cell proliferation and metabolic activity. None of the assessed in vitro microenvironment modulators affected cell viability. Anisotropic surface topography induced bidirectional cell morphology, especially on more rigid (1,000 kPa and 130 kPa) substrates. Macromolecular crowding increased various collagen types, but not fibronectin, deposition. Macromolecular crowding induced globular extracellular matrix deposition, independently of the properties of the substrate. At day 14 (longest time point assessed), macromolecular crowding downregulated tenascin C (in 9 out of the 14 groups), aggrecan (in 13 out of the 14 groups), osteonectin (in 13 out of the 14 groups), and collagen type I (in all groups). Overall, our data suggest that physicochemical cues (such surface topography, substrate rigidity, collagen coating and macromolecular crowding) are not as potent as biological signals in inducing dermal fibroblast trans-differentiation.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"11 ","pages":"Article 100079"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10499661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10289871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomaterials are the key to unlock spheroid function and therapeutic potential","authors":"David H. Ramos-Rodriguez ,&nbsp;J. Kent Leach","doi":"10.1016/j.bbiosy.2023.100080","DOIUrl":"10.1016/j.bbiosy.2023.100080","url":null,"abstract":"<div><p>Spheroids are three-dimensional cell aggregates that mimic fundamental aspects of the native tissue microenvironment better than single cells, making them a promising platform for the study of tissue development and therapeutics. Spheroids have been investigated for decades as models in cancer research, yet we have only just scratched the surface of their potential clinical utility in cell-based therapies. Like many cells, spheroids commonly exhibit a loss of key attributes upon implantation, motivating the need for strategies to regulate their function <em>in situ</em>. Biomaterials offer numerous opportunities to preserve spheroid function and guide spheroid behavior by tailoring the local microenvironment.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"11 ","pages":"Article 100080"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10499638/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10290855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun decellularized extracellular matrix scaffolds promote the regeneration of injured neurons","authors":"Lena Mungenast ,&nbsp;Ronya Nieminen ,&nbsp;Carine Gaiser ,&nbsp;Ana Bela Faia-Torres ,&nbsp;Jürgen Rühe ,&nbsp;Laura Suter-Dick","doi":"10.1016/j.bbiosy.2023.100081","DOIUrl":"10.1016/j.bbiosy.2023.100081","url":null,"abstract":"<div><p>Traumatic injury to the spinal cord (SCI) causes the transection of neurons, formation of a lesion cavity, and remodeling of the microenvironment by excessive extracellular matrix (ECM) deposition and scar formation leading to a regeneration-prohibiting environment. Electrospun fiber scaffolds have been shown to simulate the ECM and increase neural alignment and neurite outgrowth contributing to a growth-permissive matrix. In this work, electrospun ECM-like fibers providing biochemical and topological cues are implemented into a scaffold to represent an oriented biomaterial suitable for the alignment and migration of neural cells in order to improve spinal cord regeneration. The successfully decellularized spinal cord ECM (dECM), with no visible cell nuclei and dsDNA content &lt; 50 ng/mg tissue, showed preserved ECM components, such as glycosaminoglycans and collagens. Serving as the biomaterial for 3D printer-assisted electrospinning, highly aligned and randomly distributed dECM fiber scaffolds (&lt; 1 µm fiber diameter) were fabricated. The scaffolds were cytocompatible and supported the viability of a human neural cell line (SH-SY5Y) for 14 days. Cells were selectively differentiated into neurons, as confirmed by immunolabeling of specific cell markers (ChAT, Tubulin ß), and followed the orientation given by the dECM scaffolds. After generating a lesion site on the cell-scaffold model, cell migration was observed and compared to reference poly-ε-caprolactone fiber scaffolds. The aligned dECM fiber scaffold promoted the fastest and most efficient lesion closure, indicating superior cell guiding capabilities of dECM-based scaffolds. The strategy of combining decellularized tissues with controlled deposition of fibers to optimize biochemical and topographical cues opens the way for clinically relevant central nervous system scaffolding solutions.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"11 ","pages":"Article 100081"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9e/8e/main.PMC10329103.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9811693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}