Advances in biomarker sciences and technology最新文献

{"title":"Lung-brain axis-generated inflammatory biomarkers in traumatic brain injury and acute respiratory distress syndrome: Role of mechanical ventilation/stress","authors":"Nathan H. Johnson ,&nbsp;Nancy G. Casanova ,&nbsp;Susannah Patarroyo-White ,&nbsp;Jason Canizales ,&nbsp;Sara M. Camp ,&nbsp;Jon Perez Barcena ,&nbsp;Juan Pablo de Rivero Vaccari ,&nbsp;Bellal Joseph ,&nbsp;Joe G.N. Garcia","doi":"10.1016/j.abst.2025.08.002","DOIUrl":"10.1016/j.abst.2025.08.002","url":null,"abstract":"<div><h3>Rationale</h3><div>The unmet need for effective therapeutic strategies to address the bi-directional perturbation of the lung-brain axis following traumatic brain injury (TBI) or associated with Acute Lung Injury/Acute Respiratory Distress Syndrome (ALI/ARDS) is increasingly recognized. Contributing to this unmet need is the absence of reliable biomarkers that reflect the severity of lung-brain axis disruption. We assessed specific potential lung-brain axis biomarkers in TBI and ALI/ARDS subjects and explored the specific influence of exposure to mechanical ventilation.</div></div><div><h3>Methods</h3><div>Serum biomarker levels from TBI (n = 97) and ARDS subjects (n = 39) and healthy controls (n = 46) were analyzed (MesoScale Discovery ELISA) utilizing a critical illness lung-brain axis biomarker panel (CILBA) that included DAMPS (eNAMPT, S100A8), inflammatory cytokines (IL-6, IL-1β, IL-1RA, TNF-α), vascular biomarkers (PSGL-1, ANG-2), and neurotrauma biomarkers (GFAP or Glial fibrillary acidic protein, NFL or neurofilament light chain, Tau).</div></div><div><h3>Results</h3><div>TBI and ARDS subjects demonstrated significant elevations in each biomarker (compared to controls) with two exceptions: PSGL-1 was exclusively elevated in ARDS and GFAP exclusively elevated in TBI. Mechanically ventilated subjects exposed exhibited significantly DAMP, vascular and neurotrauma biomarker elevations compared to unexposed subjects. With the exception of GFAP, Ang-2, and S100A8, biomarker elevations were linked to ICU days or mortality.</div></div><div><h3>Conclusions</h3><div>These results highlight overlapping innate immunity dysregulation as a manifestation of lung-brain axis disruption in both TBI- and ARDS-exposed subjects with amplified dysregulation with mechanical ventilation. Additional longitudinal studies of well-phenotyped TBI and ARDS subjects may substantiate the prognostic value of biomarker analyses in assessing the severity of bidirectional lung-brain axis injuries.</div></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"7 ","pages":"Pages 238-247"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144841152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-driven discovery of anoikis-related biomarkers in Adult T-Cell Leukemia/Lymphoma subtypes","authors":"Mohadeseh Zarei Ghobadi,&nbsp;Elaheh Afsaneh","doi":"10.1016/j.abst.2025.06.001","DOIUrl":"10.1016/j.abst.2025.06.001","url":null,"abstract":"<div><div>Adult T-Cell Leukemia/Lymphoma (ATLL) is a malignancy that arises from T-cells infected with the human T-cell lymphotropic virus type 1 (HTLV-1). The disease is characterized by uncontrolled proliferation and reduced apoptosis of malignant T cells, which contributes to tumor progression and resistance to therapy. Anoikis is a specific form of programmed cell death triggered by the loss of cell–matrix or cell–cell adhesion, playing a critical role in preventing detached cells from surviving and forming tumors. Dysregulation of anoikis has been implicated in cancer metastasis and therapeutic resistance across various malignancies; however, its role in ATLL remains largely unexplored. To our knowledge, this is the first study to investigate anoikis-related genes in ATLL subtypes, particularly across its major subtypes: acute, chronic, and smoldering. In this study, we explored anoikis-related differentially expressed genes to identify those specifically associated with each subtype. We then applied Least Absolute Shrinkage and Selection Operator (LASSO) regression to select the most informative features. Subsequently, we employed decision trees, random forest, extreme gradient boosting, support vector machine, and logistic regression algorithms to identify classifier genes distinguishing each ATLL subtype from asymptomatic carriers. The identified biomarkers include <em>SMARCE1</em> and <em>CASP3</em> for acute, <em>TGFΒ1</em> and <em>MTA1</em> for chronic, and <em>CXCL1</em> and <em>LGALS8</em> for smoldering subtypes. These genes are involved in cell adhesion, survival signaling, and apoptosis—key processes in cellular homeostasis and oncogenesis. Our findings provide novel insights into the molecular mechanisms linking anoikis to ATLL subtypes and highlight potential therapeutic targets.</div></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"7 ","pages":"Pages 180-188"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review on the interconnection of C-reactive protein, cardiac troponins, and cytokine storm in cardiovascular manifestations of COVID-19","authors":"Poonam Sahu ,&nbsp;Trilochan Satapathy ,&nbsp;Abhisek Satapathy","doi":"10.1016/j.abst.2025.08.003","DOIUrl":"10.1016/j.abst.2025.08.003","url":null,"abstract":"<div><h3>Background</h3><div>The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been associated with substantial cardiovascular morbidity and mortality, both during acute infection and in the post-recovery phase. Identifying and understanding key biomarkers is critical for elucidating underlying mechanisms, guiding risk stratification, and informing therapeutic strategies.</div></div><div><h3>Objective</h3><div>This literature review was conducted with the aim to synthesize current evidence on the role of major cardiovascular biomarkers C-reactive protein (CRP), cardiac troponins, and pro-inflammatory cytokines in the pathophysiology, prognosis, and long-term sequelae of COVID-19.</div></div><div><h3>Methods</h3><div>The authors have gone through various databases such as <strong>-</strong> PubMed, Scopus, Web of Science, and Google Scholar. This review evaluates peer-reviewed clinical and mechanistic studies examining the association between COVID-19 between January 2020 to June 2025 with keywords and MeSH terms such as “C-Reactive Protein” OR “CRP” and “Cardiac Troponins” or “Troponin I″ or “Troponin T″ and “Cytokine Storm” and “COVID-19″ or “SARS-CoV-2″ and “Cardiovascular Complications” and cardiovascular complications, with a focus on inflammatory and myocardial injury biomarkers.</div></div><div><h3>Results</h3><div>CRP, an acute-phase reactant and sensitive indicator of systemic inflammation, is markedly elevated in severe COVID-19 and correlates with endothelial dysfunction and disease severity. Cardiac troponins, highly specific markers of myocardial injury, are significantly increased in affected patients, predicting adverse clinical outcomes and higher mortality risk. Elevated levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and other cytokines characteristic of the COVID-19–induced “cytokine storm” play a pivotal role in the pathogenesis of acute cardiac injury, myocarditis, arrhythmias, and thromboembolic events. Persistent elevation of these biomarkers in some patients after acute illness suggests ongoing myocardial stress and chronic inflammation, potentially contributing to post-acute sequelae of SARS-CoV-2 infection, including long COVID and late cardiovascular complications.</div></div><div><h3>Conclusion</h3><div>Biomarkers such as CRP, cardiac troponins, and cytokines are integral to understanding the cardiovascular impact of COVID-19, offering prognostic value for both acute and long-term outcomes. Ongoing research into targeted anti-inflammatory and cardioprotective therapies holds promise for mitigating these complications and improving patient survival and quality of life.</div></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"7 ","pages":"Pages 248-260"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time to embrace personalized medicine instead of only pursuing universality of biomarkers","authors":"Youhe Gao","doi":"10.1016/j.abst.2025.09.001","DOIUrl":"10.1016/j.abst.2025.09.001","url":null,"abstract":"","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"7 ","pages":"Pages 378-379"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Faecal microbial transplant","authors":"Onifade Isreal Ayobami, Oluwatomiwa Jubilee Sunbare-Funto, Chinedu Endurance Mbah, O. Ajibade, O. Oyawoye, A. Aborode, S. C. Ogunleye, A. Jamiu, Basit Bolarinwa, Mosope F. Abanikannda, Zainab Tiamiyu, A. R. Idowu, O. Ige, Opara Julia Kelechi, Jeremiah I. Abok, Eniola A. Lawal, Ibude Jane Aruorivwooghene, Adekunle Fatai Adeoye, Olowo Roqeebah, Emmanuel Akinloye Ojewole, R. Adesola","doi":"10.1016/j.abst.2024.02.001","DOIUrl":"https://doi.org/10.1016/j.abst.2024.02.001","url":null,"abstract":"","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"38 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139817822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miRNA99a as a Potential target in P13K/Akt1/mTOR signaling pathway in progression of OSCC","authors":"Shazia Fathima J H ,&nbsp;Selvaraj Jayaram ,&nbsp;Vishnu Priya Veeraraghavan ,&nbsp;Mohmed Isaqali Karobar","doi":"10.1016/j.abst.2024.10.003","DOIUrl":"10.1016/j.abst.2024.10.003","url":null,"abstract":"<div><h3>Background</h3><div>Oral cancer presents a significant global health challenge, driving ongoing research to enhance diagnostics and treatments. MicroRNAs, particularly miRNA99a, have emerged as key players in oral cancer's initiation, progression, and advanced development. However, their precise molecular mechanisms remain unclear. We analyze miRNA99a in modulating the PI3K/Akt1/mTOR signaling pathway within oral squamous cell carcinoma through in silico data analysis. Additionally, we examined miRNA99a levels in both oral submucous fibrosis (OSMF) and oral squamous cell carcinoma (OSCC).</div></div><div><h3>Materials and methods</h3><div>A comprehensive approach utilizing various insilico tools identified potential target genes regulated by miRNA99a and examined their interactions within the PI3K/Akt1/mTOR signaling pathway. The study involved meticulous screening, functional enrichment analyses, and network analyses to understand the regulatory networks influenced by miRNA99a. Additionally, RT-PCR was used to measure the CT levels of miR-99a in OSMF, OSCC and NM samples.</div></div><div><h3>Results</h3><div>The analysis revealed a cohort of putative target genes regulated by miRNA99a, demonstrating their involvement in crucial cellular processes linked to OSCC progression. Functional enrichment analyses highlighted the significant association of these target genes with the PI3K/Akt1/mTOR pathway, indicating their potential impact on pivotal oncogenic signaling pathways. Network analyses revealed complex regulatory networks orchestrated by miRNA99a, its action within the PI3K/Akt1/mTOR signalling pathway and influencing OSCC development. RT-PCR analysis revealed a significant downregulation of miR-99a in OSCC and OSMF samples compared to NM, with mean CT values of 39.0940 and 38.3986 respectively, versus 33.7540 in NM (p = 0.000).</div></div><div><h3>Conclusion</h3><div>miRNA99a′s potential as a crucial regulator of the PI3K/Akt1/mTOR pathway in OSCC. The identified target genes and their interactions offer a foundation for further experimental validations, presenting opportunities for discovering novel therapeutic avenues or prognostic markers in managing OSCC. Integrating multi-omics data reinforces the significance of miRNA99a-mediated regulatory mechanisms in the intricate landscape of oral cancer biology.</div></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"6 ","pages":"Pages 242-259"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inflammation-induced cellular changes: Genetic mutations, oncogene impact, and novel glycoprotein biomarkers","authors":"Xiaotong Wang ,&nbsp;Yunqiu Shen ,&nbsp;Yan Chen ,&nbsp;Shuang Yang","doi":"10.1016/j.abst.2024.06.002","DOIUrl":"https://doi.org/10.1016/j.abst.2024.06.002","url":null,"abstract":"<div><p>Persistent inflammation can trigger the development of colorectal cancer, especially in patients with inflammatory bowel disease (IBD). The precise molecular mechanisms underlying this process are not fully understood. This study investigated the molecular modifications that occur in the cellular microenvironment during inflammation-induced and colitis-associated cancers. Studies showed that genetic mutations and post-translational modifications of oncogene proteins can alter the biological functions of macrophage inflammatory proteins, complicating the intricate interactions between inflammation and cancer. The researchers also observed abnormal glycosylation patterns in cases of inflammation and colitis-associated cancers. This observation suggests that glycoproteins present in bodily fluids could potentially serve as valuable disease markers. Additionally, the researchers investigated general signaling alterations that manifest in cases of colitis-associated cancer. They proposed a provisional molecular model that suggests the involvement of endoplasmic reticulum (ER) stress during the transition from inflammation to cancer. This potential pathway is mediated through the FKBP/c-Myc/p53 signaling axis. In the context of protein glycosylation, we summarize the potential molecular mechanisms of IBD-induced carcinogenesis. This knowledge could potentially lead to the development of novel targets for the clinical treatment of colorectal cancer.</p></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"6 ","pages":"Pages 91-104"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2543106424000097/pdfft?md5=718031484392d22ce87e2421b57cf8f9&pid=1-s2.0-S2543106424000097-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141438577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Matrix metalloproteinase 7 as a diagnostic biomarker of biliary atresia: A systematic review","authors":"Pauline Louise Møllmann Lausten ,&nbsp;Vibeke Brix Christensen ,&nbsp;Hannelouise Kissow","doi":"10.1016/j.abst.2024.04.001","DOIUrl":"10.1016/j.abst.2024.04.001","url":null,"abstract":"<div><h3>Background</h3><p>Biliary atresia (BA) is a disease of the intrahepatic or extrahepatic bile ducts with an unknown etiology. It presents in neonates with jaundice, clay-colored stool, and often hepatomegaly. Early diagnosis of the disease is pivotal for long-term prognosis. If the BA is left untreated, progressive liver cirrhosis and death can occur. Persisting jaundice in infants born at term should lead to further examination of liver diseases. A range of laboratory analyses is used, but none is specific for BA. In this review, we investigate whether the level of matrix metalloproteinase 7 (MMP-7) in serum can be used as an early diagnostic biomarker for BA.</p></div><div><h3>Method</h3><p>A systematic literature search of the PubMed database revealed the two terms “matrix metalloproteinase 7” and “biliary atresia”. A total of 24 articles were identified; these articles were screened, and eight articles were found to be relevant for this literature review, each describing an independent study.</p></div><div><h3>Results</h3><p>In all eight articles, the diagnostic cut-off values for serum MMP-7 in BA patients vs. non-BA patients were determined by receiver operating characteristic (ROC) curve analysis and by determining the area under the curve (AUC). The AUC ranged from 0.96 to 0.99. All studies had a sensitivity of 95 % or above and a specificity of 83 % or above. The cut-off values were discordant and ranged from 1.43 ng/ml to 52.85 ng/ml. The calculated positive likelihood ratio (PLR) varied from 5.66 to 21.86, and the negative likelihood ratio (NLR) varied from 0.01 to 0.05 among the eight studies. Finally, the diagnostic odds ratio (DOR) varied from 168.64 to 1406.00 in seven out of the eight studies.</p></div><div><h3>Conclusion</h3><p>The serum MMP-7 concentration can be used as a diagnostic biomarker according to the eight studies investigated in this review. However, further assessments of MMP-7 in larger, multicenter, and multiethnic studies are needed to validate its potential for biomarker development and, ultimately, its standard use in clinical practice.</p></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"6 ","pages":"Pages 72-82"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2543106424000061/pdfft?md5=af95e4928e71a8f2814b306292f1fb85&pid=1-s2.0-S2543106424000061-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140771555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and applications of different types of green biosorbents for eliminations of hardness from water: A review on treatment, kinetics mechanism and future scope","authors":"Subhashish Dey,&nbsp;G.T.N. Veerendra,&nbsp;A.V. Phani Manoj,&nbsp;Siva Shanmukha Anjaneya Babu Padavala,&nbsp;A.H.L. Swaroop","doi":"10.1016/j.abst.2024.11.001","DOIUrl":"10.1016/j.abst.2024.11.001","url":null,"abstract":"<div><div>The presence of toxic materials in water solutions, mainly harmful metals and metalloids, is a significant ecological and community issue. In village areas, hardness is major groundwater toxicity. Hardness, those are alkaline in nature, can reason problems to the eyes, respiratory tract failure, and epidermis in both its liquid and gaseous types. The natural parts of hardness in humans subsequent to severe exposures are concentrations-dependent and changes depending on the duration, times of effects, and quantity absorbed by the body. Biosorption is a physiochemical processes that happens mainly in the certain biomass, following it to inactively collect and attach impurities onto its cellular configuration. It is a metabolically inactive procedure that does not need energy. The composition and kinetic equilibrium of the sorbent's cellular surface determine the number of contaminants that the sorbent can eliminate. The biosorption procedures were facilitate by the single physical, biological and chemical characteristics of each biosorbents those applied the elimination of hardness from the water. In the hardness of the water has been removed, the biosorption procedures can be made highly cost-effective by recycling and reprocessing of the biosorbents. The removals of hardness from huge amounts of water can be done by the applications of a variety of bioreactors in the biosorption.</div></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"6 ","pages":"Pages 265-299"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142652860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of black seed oil in treating rheumatoid arthritis: Insights on biochemical and genetic analyses of inflammatory signaling transducer","authors":"Mohamed G. Elharrif ,&nbsp;Omnia M. Abdelhamid ,&nbsp;Hussein A. Abdel-Maksoud ,&nbsp;Naglaa F. Alhusseini ,&nbsp;Nihal E. Amer","doi":"10.1016/j.abst.2024.09.002","DOIUrl":"10.1016/j.abst.2024.09.002","url":null,"abstract":"<div><div>Nigella sativa (Black seed) has been a longstanding component of traditional medicine. Rheumatoid arthritis, an autoimmune disorder targeting bone, cartilage, and synovial cells, leads to enduring joint damage due to pathological immune responses. This study aims to assess the effectiveness of black seed oil in treating rheumatoid arthritis using a rat model. Fifty male albino rats were randomly assigned to five groups: control, induced arthritis, induced arthritis treated with black seed oil, induced arthritis treated with hydroxychloroquine, and induced arthritis treated with a combination of black seed oil and hydroxychloroquine. Arthritis was induced in the rats using single dose of 0.1 ml of Complete Freunds Adjuvant. Biochemical and genetic analyses of signaling transducers (IFNγ, IL-4, STAT3, and IL-12) were conducted on blood samples and stifle joints. Results indicated that induced arthritis led to elevated serum AST, ALT activities, creatinine, urea, FBS, RF, Anti-CCP, and CRP, along with reduced serum TAC. Additionally, there was an upregulation of IL-12, IFNγ, and STAT3 genes, and a downregulation of IL-4 gene compared to the normal control group. Treatment with black seed oil or hydroxychloroquine individually demonstrated notable improvements in these parameters, although the combined treatment showed less marked improvement. In conclusion, black seed oil demonstrates potential therapeutic benefits in alleviating the negative impacts of rheumatoid arthritis.</div></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"6 ","pages":"Pages 217-225"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142424717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}