Advances in biomarker sciences and technology最新文献

{"title":"Faecal microbial transplant","authors":"Isreal Ayobami Onifade ,&nbsp;Oluwatomiwa Jubilee Sunbare-Funto ,&nbsp;Chinedu Endurance Mbah ,&nbsp;Oluwatosin Akinola Ajibade ,&nbsp;Olubukola Monisola Oyawoye ,&nbsp;Abdullahi Tunde Aborode ,&nbsp;Seto Charles Ogunleye ,&nbsp;Abdullahi Jamiu ,&nbsp;Basit Bolarinwa ,&nbsp;Mosope F. Abanikannda ,&nbsp;Zainab Tiamiyu ,&nbsp;Abidemi Ruth Idowu ,&nbsp;Oluwatobiloba Ige ,&nbsp;Opara Julia Kelechi ,&nbsp;Jeremiah I. Abok ,&nbsp;Eniola A. Lawal ,&nbsp;Ibude Jane Aruorivwooghene ,&nbsp;Adekunle Fatai Adeoye ,&nbsp;Olowo Roqeebah ,&nbsp;Emmanuel Akinloye Ojewole ,&nbsp;Ridwan Olamilekan Adesola","doi":"10.1016/j.abst.2024.02.001","DOIUrl":"10.1016/j.abst.2024.02.001","url":null,"abstract":"<div><p>Faecal Microbial Transplantation (FMT), have been shown to be successfully managed along with a variety of gastrointestinal disorders, including <em>Clostridioides difficile</em> infection (CDI), inflammatory bowel disease (IBD), and irritable bowel syndrome (IBS). FMT is a medical procedure that involves a donor transferring healthy gut bacteria to the recipient. The effectiveness of FMT is thought to be due to the ability of the healthy gut to restore balance to the recipient's microbiome, which is the community of microorganisms that inhabit the gastrointestinal tract. One of the key advantages of FMT is that it is a relatively simple and low-cost treatment option, compared to other therapies that may be more invasive or require long-term medication use. However, FMT is not without its limitations, including the potential for the transfer of infectious agents from the donor to the recipient and the potential for negative side effects in some patients. In this research, we discuss the current state of the evidence on FMT, including its potential benefits and risks, as well as future directions for research in this area.</p></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"6 ","pages":"Pages 20-34"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2543106424000036/pdfft?md5=2c2f7c6deba6132e414ddec7623c4461&pid=1-s2.0-S2543106424000036-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139877860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of cognitive computing in enhancing innovative healthcare solutions","authors":"Abid Haleem,&nbsp;Mohd Javaid","doi":"10.1016/j.abst.2024.08.002","DOIUrl":"10.1016/j.abst.2024.08.002","url":null,"abstract":"<div><p>Cognitive computing represents a groundbreaking development in healthcare, encompassing technological platforms that emulate the human brain's functionality. While cloud computing offers on-demand internet access to computing resources and services, cognitive computing focuses on modelling human mental processes to tackle complicated issues. Cognitive computing enhances human decision-making by integrating reasoning, machine learning, speech, natural language processing (NLP), and human-computer interaction. In the healthcare sector, it facilitates the analysis of clinical and genetic data to forecast diseases, tailor therapies, and elevate drug development. Additionally, it combines data analysis with adaptive page displays to tailor content based on the audience. Relevant papers in cognitive computing for healthcare were identified and studied. This paper aims to undertake an extensive scopic review of the pertinent literature from various sources, including articles and documents from numerous journals and conference proceedings. It delves into the need for cognitive computing in healthcare, elucidates supportive technologies, and expounds on its features within the healthcare domain. Furthermore, it identifies and discusses the substantial applications of cognitive computing in healthcare. These systems utilise computer models to replicate human cognitive processes, streamlining administrative tasks through artificial intelligence and cognitive computing. As a result, healthcare administrators can allocate more of their valuable time to patient care. Cognitive computing enhances outcomes and practitioner productivity and improves treatment decisions. The self-learning system of cognitive computing relies on real-time patient data, medical transcripts, and other pertinent information. These technologies can reduce the administrative burden on healthcare workers by automating tasks such as invoicing, claims processing, and appointment scheduling. This technology is poised to become increasingly indispensable in precision medicine.</p></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"6 ","pages":"Pages 152-165"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2543106424000139/pdfft?md5=cb7d5fbc41d9253461511cb165aed156&pid=1-s2.0-S2543106424000139-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142088999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patient-derived primary culture-based prognostic model to predict tumor recurrence in patients with glioma","authors":"Syed Sultan Beevi ,&nbsp;Manas Kumar Panigrahi ,&nbsp;Vinod Kumar Verma ,&nbsp;Jyotsana Dwivedi ,&nbsp;Sailaja Madigubba ,&nbsp;Radhika Chowdary Darapuneni ,&nbsp;Seema M. Gafurjiwala ,&nbsp;Sambit Sahu ,&nbsp;Bhaskar Rao Bollineni","doi":"10.1016/j.abst.2024.01.002","DOIUrl":"https://doi.org/10.1016/j.abst.2024.01.002","url":null,"abstract":"<div><h3>Background</h3><p>The prognosis for glioma patients remains grim despite aggressive treatment approaches. Current molecular profiles have limitations in predicting glioma recurrence, highlighting the need for new and improved prognostic biomarkers. We investigated whether the growth kinetics of patient-derived glioma cultures (PDGCs) can offer valuable prognostic insights to predict tumor recurrence. Additionally, we examined the expression of glial-mesenchymal transition (GMT) markers in PDGCs to assess their potential as additional prognostic biomarkers.</p></div><div><h3>Methods</h3><p>130 patients diagnosed with primary glioma via MRI scans were prospectively enrolled. Surgical tumor tissues were collected from all participants and used to establish patient-derived glioma cultures (PDGCs). The growth kinetics and colony-forming ability of the respective PDGCs were calculated to derive proliferation index (PI) for each patient. Progression-free survival (PFS) and overall survival (OS) served as the primary outcome measures.</p></div><div><h3>Results</h3><p>We established short-term glioma cultures in 98 clinical samples, regardless of the CNS WHO tumor grade, IDH1/2 mutation and 19/19q codeletion status and maintained active cell proliferation for at least 10–12 passages. However, we observed two distinct growth kinetic patterns among PDGCs. Based on their proliferation index (PI), we categorized patients into either high proliferation index (HPI) or low proliferation index (LPI) group. Furthermore, we noted a differential expression profile of GMT markers between HPI and LPI patients. The proliferation index (PI) exhibited a significant correlation with progression-free survival (PFS), while the expression of GMT marker vimentin was associated with overall survival (OS).</p></div><div><h3>Conclusion</h3><p>The PDGC-derived Proliferation Index (PI) can serve as a predictive tool for tumor recurrence, independent of clinical or tumor-related factors. Moreover, reduced vimentin expression is a positive indicator for glioma patients' overall survival status.</p></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"6 ","pages":"Pages 8-19"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2543106424000024/pdfft?md5=96223fcb0f1a5608cce72f2403943389&pid=1-s2.0-S2543106424000024-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139700168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"World urine project","authors":"Youhe Gao MD/PhD,&nbsp;Tatjana Crnogorac-Jurcevic MD/ PhD,&nbsp;Bogdan Budnik PhD","doi":"10.1016/j.abst.2024.10.002","DOIUrl":"10.1016/j.abst.2024.10.002","url":null,"abstract":"","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"6 ","pages":"Page 226"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142535470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of cytotoxic and antioxidant biomarkers from the aerial parts of Suaeda aegyptica","authors":"Nimra Nadeem Ahmed ,&nbsp;Salem Said Jaroof Al-Touby ,&nbsp;Yahya Bin Abdullah Alrashdi ,&nbsp;Mohammad Amzad Hossain","doi":"10.1016/j.abst.2024.01.001","DOIUrl":"https://doi.org/10.1016/j.abst.2024.01.001","url":null,"abstract":"<div><p>Chronic disease is an irregular change inside or outside of the tissues and organs that results in accumulation, local damage, and inflammation or irritation. <em>Suaeda aegyptiaca</em> (<em>S. aegyptiaca</em>) is a plant that has been used for a long time for the treatment of human diseases. Therefore, the aim of this present research study is to prepare various plant extracts and screen their antioxidant activity spectroscopically, and later on to isolate antioxidant biomarkers from the significantly highest active crude extract of the aerial parts of <em>S. aegyptiaca.</em> To attain the present objectives, different crude extracts were prepared from the aerial parts of <em>S. aegyptiaca</em> by using a maceration method. The antioxidant and cytotoxic activities of the prepared aerial crude extracts of <em>S. aegyptiaca</em> were determined by 2,2-diphenyl-1-1-picrylhydrazyl (DPPH) and brine shrimp lethality (BSL) methods, respectively. All the prepared aerial crude extracts of the particular plant at six different concentrations showed significant antioxidant activity against the DPPH. The ethyl acetate crude extract showed the highest antioxidant activity, and the lowest activity was in the butanol extract. However, all the aerial crude extracts of <em>S. aegyptiaca</em> were prepared at different concentrations did not show any activity against the BSL method. Based on the antioxidant activity results, the ethyl acetate extract was selected for the isolation of antioxidant compounds. The extract was purified using column chromatography by using different solvent ratios. A series of test tubes were collected with a volume of 3 mL and depending on the similar retention mobility (R_f) behavior, a total of twelve fractions were prepared. Similarly, the antioxidant activity of the obtained twelve fractions from column chromatography was determined by the same DPPH method. All the fractions showed significant antioxidant activity. Among the fractions from the column, fraction 6 gave the highest antioxidant activity and the lowest was fraction 1. In conclusion, all the aerial extracts showed promising activities against DPPH and the fraction with the highest antioxidant activity could be used as natural antioxidant biomarkers to prevent cell damage.</p></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"6 ","pages":"Pages 1-7"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2543106424000012/pdfft?md5=cd76366294c14dc9d8e67ff730908aea&pid=1-s2.0-S2543106424000012-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139548627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming early microbial detection: Investigating innovative biosensors for emerging infectious diseases","authors":"Godfred Yawson Scott ,&nbsp;Abdullahi Tunde Aborode ,&nbsp;Ridwan Olamilekan Adesola ,&nbsp;Emmanuel Ebuka Elebesunu ,&nbsp;Joseph Agyapong ,&nbsp;Adamu Muhammad Ibrahim ,&nbsp;ANGYIBA Serge Andigema ,&nbsp;Samuel Kwarteng ,&nbsp;Isreal Ayobami Onifade ,&nbsp;Adekunle Fatai Adeoye ,&nbsp;Babatunde Akinola Aluko ,&nbsp;Taiwo Bakare-Abidola ,&nbsp;Lateef Olawale Fatai ,&nbsp;Osasere Jude-Kelly Osayawe ,&nbsp;Modupe Oladayo ,&nbsp;Abraham Osinuga ,&nbsp;Zainab Olapade ,&nbsp;Anthony Ifeanyi Osu ,&nbsp;Peter Ofuje Obidi","doi":"10.1016/j.abst.2024.04.002","DOIUrl":"10.1016/j.abst.2024.04.002","url":null,"abstract":"<div><p>The recent global pandemic has highlighted an increase in the prevalence of communicable diseases caused by pathogens. The swift transmission of these diseases within a short timeframe presents a substantial risk to public health worldwide. The inefficiency of traditional diagnostic instruments, which need a time-consuming and complex process in the laboratory, is a significant obstacle to medical care. Currently, there is a high need for the advancement of early detection in order to rapidly diagnose infectious diseases and provide on-site results. This is crucial for prompt and early intervention to improve treatment outcomes. This also provides rapid testing and high-quality microbiological detection, comparable to laboratory standards, in a matter of minutes. Prompt diagnosis and subsequent treatment optimization aid in controlling the spread of infectious diseases. Currently, ongoing techniques and methods are used in the advancements of early detection through biosensors. This review examines the integration of early diagnostics with biosensors, specifically in relation to emerging and re-emerging infectious diseases, challenges, and the future perspective.</p></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"6 ","pages":"Pages 59-71"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2543106424000073/pdfft?md5=8d4daf6f53f265b4ce4d0091b9c4bba7&pid=1-s2.0-S2543106424000073-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140789933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the effects of polyherbal formulations against the combination of a high-fat cafeteria diet along with exercise modalities","authors":"M. Ramasamy ,&nbsp;E. Karthikeyan ,&nbsp;S. Srinivasan ,&nbsp;S. Navaneetha Krishnan","doi":"10.1016/j.abst.2024.02.002","DOIUrl":"https://doi.org/10.1016/j.abst.2024.02.002","url":null,"abstract":"<div><p>The aim of the present study was to make a polyherbal formulation of three herbs that would offset the negative effects of the high-fat cafeteria diet on rats and the beneficial effects of exercise. The ingredients for this herbal remedy included a water extract of Garcinia cambogia fruit, Trigonella foenum-graecum seed, and Glycyrrhiza glabra root in a 1:1:1 ratio. Nine groups of six male Wistar albino rats were chosen. Animals in Group I were normal and fed a standard diet; animals in Group II were given HFCD to prevent disease; animals in Group III were given HFCD plus exercise; while animals in Groups IV and V were given PHF at doses of 400 and 200 mg/kg p. o. Daily, respectively, in addition to HFCD; while animals in Groups VI and VII were exercised obese rats prevented from gaining more weight had their serum glucose, total cholesterol, triglycerides, low-density lipoprotein cholesterol, and very low-density lipoprotein cholesterol levels lowered. HDL cholesterol was raised. Further, the liver biopsy showed increased mononuclear cells; fat globules numbered less; and the enzymes and cells that are regenerating were at lower levels. The polyherbal formulation plus exercise exhibited significant anti-obesity efficacy at 400 mg/kg p. o.; the effect was equivalent to orlistat plus exercise. The research employed HFCD-induced obese rats as a model to investigate the joint effects of PHF and exercise against obesity.</p></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"6 ","pages":"Pages 35-45"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2543106424000048/pdfft?md5=e61a3ff043e0f45eb1cbdf57e209333e&pid=1-s2.0-S2543106424000048-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139975960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to editor in chief “Taurine deficiency as a driver of aging”","authors":"Animesh Acharjee","doi":"10.1016/j.abst.2023.11.001","DOIUrl":"https://doi.org/10.1016/j.abst.2023.11.001","url":null,"abstract":"","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"5 ","pages":"Page 124"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2543106423000121/pdfft?md5=4af9e578196d1b2c31c56b3ebe5ef09f&pid=1-s2.0-S2543106423000121-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134657173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic performance of point-of-care ubiquitin carboxy-terminal Hydrolase-L1 assay in distinguishing imaging abnormalities in traumatic brain injury: A TRACK-TBI cohort study","authors":"Kevin K. Wang ,&nbsp;Jennifer C. Munoz-Pareja ,&nbsp;Lauren A. Lautenslager ,&nbsp;J. Adrian Tyndall ,&nbsp;Zhihui Yang ,&nbsp;Maria R. Kerrigan ,&nbsp;Ramon Diaz-Arrastia ,&nbsp;Frederick K. Korley ,&nbsp;David Okonkwo ,&nbsp;Ava M. Puccio ,&nbsp;John K. Yue ,&nbsp;Sabrina R. Taylor ,&nbsp;Pratik Mukherjee ,&nbsp;Esther L. Yuh ,&nbsp;Nancy R. Temkin ,&nbsp;Claudia S. Robertson ,&nbsp;Xiaoying Sun ,&nbsp;Sonia Jain ,&nbsp;Amy J. Markowitz ,&nbsp;Geoffrey T. Manley ,&nbsp;Ross Zafonte","doi":"10.1016/j.abst.2023.04.002","DOIUrl":"10.1016/j.abst.2023.04.002","url":null,"abstract":"<div><p>The use of UCH-L1 detection with point-of-care (POC) assay alone has not been characterized for clinical use. This study compares the accuracies of POC UCH-L1 and Neuron-Specific Enolase (NSE) Elecsys® levels for identifying TBI patients with structural abnormalities on neuroimaging.</p><p>The <u>T</u>ransforming <u>R</u>esearch and <u>C</u>linical <u>K</u>nowledge in <u>T</u>raumatic <u>B</u>rain <u>I</u>njury (TRACK-TBI) Phase 1 Cohort, enrolled 1375 TBI patients (GCS 3–15) presenting to one of 18 US Level I trauma centers within 24 h of injury who had an admission head CT; blood samples were collected, along with 122 orthopedic and 209 healthy controls. The TBI cohort consisted of 810 CT-negative (CT-) and 549 CT-positive (CT+) subjects. Of the CT- subjects who had MRIs, 121 were MRI-positive (MRI+) and 333 were MRI-negative (MRI-). UCH-L1 POC showed best diagnostic performance for CT + versus CT-, 0–8 h post-injury with an AUC of 0·779 [0·708–0.850] when compared to the 0–25 h interval, with an AUC of 0.684 [0.655–0.712]. NSE assay has an AUC of 0.695 [0.619–0.770] for the 0–8 h interval and 0.634 [0.603–0.665] for the 0–25 h interval. During the first 8 after injury, POC UCH-L1 outperforms NSE in identifying TBI patients with structural abnormalities on neuroimaging.</p></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"5 ","pages":"Pages 38-49"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44842807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic capability of selected medicinal plants' bioactive constituents against the mutant ovarian TP53 gene; a computational approach","authors":"Kayode Yomi Raheem ,&nbsp;Fawehinmi Praise Ibukunoluwa ,&nbsp;Solomon Ayodele Olorundare ,&nbsp;Jairus Olumasai Nandwa ,&nbsp;Modinat Aina Abayomi ,&nbsp;Egbe Justine Uchechukwu ,&nbsp;Mary Adewunmi ,&nbsp;Kuyet Zichat Blessing ,&nbsp;Modupe Mercy Anthony ,&nbsp;Mary Ikeoluwa Gbadebo ,&nbsp;Falana Taiwo Daniel","doi":"10.1016/j.abst.2023.02.001","DOIUrl":"10.1016/j.abst.2023.02.001","url":null,"abstract":"<div><h3>Background</h3><p>A mutant P53 protein plays such a crucial role in ovarian cancer, and natural compounds have been known to be effective in treating cancer. The current study was conducted to discover new mutant P53 modulators in plants used for medicinal purposes. The mutant p53 3D structure was built using homology modeling, while its active binding domain was predicted using Findsitecom2.0. Docking studies were conducted with ligands derived from bioactive components of seven different plants and mutant p53 binding sites. Autodocking programs, including Discovery Studio and PyRx, were used to obtain the docking protein and its intricate visual representation. Gemcitabine and thiotepa were the reference drugs. Acute RAT toxicity and Pharmacokinetic properties were utilized in Gusar and SWISSADME, respectively, to narrow down the hit compounds to those with the highest binding affinities. Using the density functional theory (DFT) method, the electronic properties of the bioactive constituents were determined. 15 of the 50 bioactive phytochemicals displayed superior mutant p53 binding energies compared to Gemcitabine and Thioteba (−5.4 and −3.5 binding scores, respectively). Considering acute toxicity predictions and pharmacokinetics, 10-hydroxycamptothecin, irinotecan, morusin, and rubitecan were the four major compounds with low toxicity. DFT calculations uncovered regions susceptible to nucleophilic and electrophilic assaults. The study sought to identify potential drug candidates for modulating mutant P53 in ovarian cancer treatment.</p></div>","PeriodicalId":72080,"journal":{"name":"Advances in biomarker sciences and technology","volume":"5 ","pages":"Pages 8-32"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45541897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}