Acta virologica最新文献_第8页

Natural infection of purple passion fruit (Passiflora edulis f. edulis) by a novel member of the family Tymoviridae in Colombia. 哥伦比亚Tymoviridae科新成员对紫百香果(Passiflora edulis f. edulis)的自然感染。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_310

Daniela Cardona, Andrea Restrepo, Mónica Higuita, Yuliana Gallo, Mauricio Marin, Pablo Gutiérrez

{"title":"Natural infection of purple passion fruit (Passiflora edulis f. edulis) by a novel member of the family Tymoviridae in Colombia.","authors":"Daniela Cardona, Andrea Restrepo, Mónica Higuita, Yuliana Gallo, Mauricio Marin, Pablo Gutiérrez","doi":"10.4149/av_2022_310","DOIUrl":"https://doi.org/10.4149/av_2022_310","url":null,"abstract":"Purple passion fruit is one of the most important fruit exports of Colombia, but its productivity is being compromised by the emergence of several viral diseases. High-throughput sequencing (HTS) surveys of viruses in purple passion fruit fields in the province of Antioquia suggested infection by a new member of the family Tymoviridae. In this work, we characterize the complete genome sequence of this virus, tentatively named purple passionfruit leaf deformation virus (PpLDV), and evaluate its distribution in Antioquia. PpLDV was assembled at high coverage in four datasets from different regions. The 6.1 kb genome of PpLDV encodes a single polyprotein with domains characteristic of the family Tymoviridae, contains a marafibox-like promoter and the 3'-UTR can fold into a tRNA-like secondary structure with a valine anti-codon. Phylogenetic analysis of the polyprotein revealed that PpLDV is a distinct member of the family Tymoviridae, more closely related to the genus Tymovirus and the unclassified Poinsettia mosaic virus (PnMV). The presence of PpLDV was confirmed by RT-qPCR and RT-PCR in samples from commercial purple passion fruit fields, plantlets and seed sprouts collected in Antioquia using primers designed in this study. Keywords: high-throughput sequencing; Marafivirus; Passifloraceae; plant virology; RT-qPCR; Tymovirus.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 3","pages":"254-262"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40447095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated view of protein homologue groups and functional genomic development of baculoviruses: towards understanding baculoviral infection mechanism and improving baculoviral expression vector 杆状病毒蛋白同源群和功能基因组发育的综合研究:对杆状病毒感染机制的理解和杆状病毒表达载体的改进

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_405

Weiping Lin, Yanhua Shi, T. Yu, Yujiao Wang, Yonglin Tan, Punan Zhao, Guohui Wang, Dianhai Hou

引用次数: 0

Identification of dicistro-like viruses in the transcriptome data of Striga asiatica and other plants. 亚洲曲带及其他植物转录组数据中双盘状病毒的鉴定。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_205

Chaerim Shin, Dongjin Choi, Ken Shirasu, Yoonsoo Hahn

引用次数: 5

Development of RT-PCR for rapid detection of ssRNA ambi-like mycovirus in a root rot fungi (Armillaria spp.). 快速检测根腐菌(蜜环菌)ssRNA双歧样分枝病毒的RT-PCR方法的建立。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_308

Tomáš Tonka, Lucie Walterová, Vladislav Čurn

引用次数: 0

Dwarf polish wheat hosts a novel closterovirus: Revelation by transcriptome data-mining. 矮波兰小麦宿主一种新的clostervirus:通过转录组数据挖掘的启示。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_207

Venkidusamy Kavi Sidharthan, Virendra Kumar Baranwal

引用次数: 1

Evaluation of the effects of 1,25VitD3 on Th17 cells and Tregs in HTLV-1 infected cell lines. 1,25维生素d3对HTLV-1感染细胞系中Th17细胞和Tregs的影响

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_202

Qing Huang, Zhijie Ling, Huabin Wang, Xiaoming Zhong, Kaiyuan Luo

{"title":"Evaluation of the effects of 1,25VitD3 on Th17 cells and Tregs in HTLV-1 infected cell lines.","authors":"Qing Huang, Zhijie Ling, Huabin Wang, Xiaoming Zhong, Kaiyuan Luo","doi":"10.4149/av_2022_202","DOIUrl":"https://doi.org/10.4149/av_2022_202","url":null,"abstract":"Human T-lymphotropic virus type 1 (HTLV-1) causes chronic infections of human T lymphocytes. The present study aimed to evaluate the effects of 1,25VitD3 on the proportion of Tregs and Th17 cells, the expression of related transcription factors (ROR-γt and FOXP3) and cytokines (IL-10, TGF-β, IL-6, and IL-17 A) in the HTLV-1infected cell lines MT-2 and MT-4. MT-2 and MT-4 cells and control PBMCs were treated with 1,25VitD3 and percentages of Tregs and Th17 cells was determined by flow cytometry. Gene expression and cytokine levels were analyzed by real-time PCR and ELISA, respectively. Treatment with-1,25VitD3 increased the percentage of Tregs in MT-2 and MT-4 cells, while it decreased the percentage of Th17 cells among MT-2 cells. 1,25VitD3 treatment also significantly improved FOXP3 gene expression in MT-2 cells, while reducing ROR-γt-gene expression in MT-2 and MT-4 cells comparing to untreated cells. Treatment with 1,25VitD3 significantly improved IL-10 levels in MT-2 cells, as well as TGF-β levels in both cell lines culture supernatants. 1,25VitD3 treatment diminished IL-6 levels in cell culture supernatants of MT-2 and MT-4 as well as IL-17 A levels in MT-2. Here we showed, that 1,25VitD3 modulated immune responses by enhancing Tregs differentiation and functions as well as inhibiting Th17 differentiation and actions in HTLV-1 infected cell lines. This suggests that VitD3 may have therapeutic effects in HTLV-1-related diseases by suppressing adverse inflammatory responses. Keywords: Tregs; Th17 cells; HTLV-1; 1, 25VitD3.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 2","pages":"127-138"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40408801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparative analysis of HBV basic core promoter/pre-core gene mutations and viral quasispecies diversity in HIV/HBV co-infected and HBV mono-infected patients. HIV/HBV合并感染与HBV单一感染患者HBV基本核心启动子/前核心基因突变及病毒准种多样性的比较分析

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_103

Haohui Deng, Hongbo Gao, Yu Liu, Ying Xu, Juncheng Yang, Miaoxian Zhao, Huiyuan Liu, Zhanhui Wang

{"title":"Comparative analysis of HBV basic core promoter/pre-core gene mutations and viral quasispecies diversity in HIV/HBV co-infected and HBV mono-infected patients.","authors":"Haohui Deng, Hongbo Gao, Yu Liu, Ying Xu, Juncheng Yang, Miaoxian Zhao, Huiyuan Liu, Zhanhui Wang","doi":"10.4149/av_2022_103","DOIUrl":"https://doi.org/10.4149/av_2022_103","url":null,"abstract":"Human immunodeficiency virus (HIV)/hepatitis B virus (HBV) co-infection accelerates the progression of HBV-related liver diseases. HBV basic core promoter (BCP)/pre-core (preC) gene mutations may be one of the most important risk factors. In this study, a total of 230 patients were recruited, and 199 patients whose HBV BCP/preC gene were successfully amplified and sequenced, including 99 HIV/HBV co-infected and 100 HBV mono-infected patients. Next-generation sequencing was used for detection of BCP/preC mutations which were then compared in patients with different HBV genotypes and different HBeAg statuses, and 1% and 20% cutoff values were defined to evaluate the mutations. HBV quasispecies diversity was also compared in HIV/HBV co-infected and HBV mono-infected patients. Among the patients infected with HBV genotype C and HBeAg-negative status, the frequency of A1762T/G1764A double mutations was significantly lower in HIV/HBV co-infected patients than in HBV mono-infected patients (53.3% vs. 100.0%, P = 0.008) regardless of the 1% or 20% cutoff value level. However, A1762T/G1764A double mutations did not differ in the other groups (P >0.05). Viral quasispecies diversity was lower in HIV/HBV co-infected patients than in HBV mono-infected patients (P Keywords: human immunodeficiency virus, hepatitis B virus; mutations; viral quasispecies; next-generation sequencing.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 1 1","pages":"18-26"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70876895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A novel approach for estimation of anti-FMDV protective immunity generated in multiple vaccinated field animals. 一种估算多种接种动物抗口蹄疫保护性免疫的新方法。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_110

B. Jain, Anuj Tewari, M. Prasad, S. K. Kadian

{"title":"A novel approach for estimation of anti-FMDV protective immunity generated in multiple vaccinated field animals.","authors":"B. Jain, Anuj Tewari, M. Prasad, S. K. Kadian","doi":"10.4149/av_2022_110","DOIUrl":"https://doi.org/10.4149/av_2022_110","url":null,"abstract":"In this study, forty serum samples from field buffaloes vaccinated with inactivated foot-and-mouth disease (FMD) vaccine were collected. These animals were multiple vaccinated with the above vaccine during previous years. The study was conducted to determine the actual status of the protective antibodies generated after vaccination. Initially, the serum samples were tested by Liquid phase blocking ELISA (LPBE), and only samples with titer more than 1.4 in LPBE were chosen for further analysis. These samples were tested with an in-house Gold Nanoparticle-based test for detection of anti-FMDV structural protein antibodies, in which the antibodies were detected at 10-4 dilution; this was suggestive of strong antibody titer generated post-vaccination. To test the binding affinity of these antibodies with the antigen, an avidity ELISA was developed and outcomes were expressed in terms of avidity index (AI). It was found that the avidity was low in some of the animals even after multiple vaccinations. Therefore, multiple vaccinations and strong antibody titer generation may not be the actual indicator of the protective immune response generated. We conclude that avidity ELISA can be a better approach than LPBE to measure the level of protective antibodies generated post-vaccination. Keywords: avidity ELISA; foot-and-mouth disease; post-vaccination monitoring; herd immunity; PCP-FMD.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"71 1","pages":"90-94"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70877287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

RPS3-induced antiviral cytokines inhibit the proliferation of classical swine fever virus. rps3诱导的抗病毒细胞因子抑制猪瘟病毒的增殖。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_107

Di Zhao, Liang Zhang, Mengzhao Song, Yanming Zhang Mail

{"title":"RPS3-induced antiviral cytokines inhibit the proliferation of classical swine fever virus.","authors":"Di Zhao, Liang Zhang, Mengzhao Song, Yanming Zhang Mail","doi":"10.4149/av_2022_107","DOIUrl":"https://doi.org/10.4149/av_2022_107","url":null,"abstract":"Classical swine fever virus (CSFV) infection results in serious economic losses to the pig industry. This positive-sense RNA virus hijacks cell host proteins for its own replication. Although previous studies have shown that RPS3, a 40S ribosomal subunit protein, is mainly required for DNA repair, apoptosis and inflammation, the effect of RPS3 on CSFV replication remains uncertain. Thus, we investigated the potential role of RPS3 in CSFV infection in RPS3-knockdown and -overexpressing cell lines using real-time fluorescence quantitative PCR (RT-qPCR) and indirect immunofluorescence assays. Results showed that knockdown of RPS3 by lentiviruses enhanced CSFV replication, whereas overexpression of RPS3 by lentiviruses inhibited CSFV replication. These findings indicated the antiviral role of RPS3 in CSFV infection. Subsequent experiments revealed that CSFV replication was inhibited in cells cultured with the supernatants of RPS3-overexpressing cell, suggesting that the RPS3-mediated inflammatory response was involved in CSFV infection. Furthermore, enzyme-linked immunosorbent assay (ELISA) revealed that the secretion of antiviral cytokines (IL-8 and INF-β) was increased in cells with sufficient RPS3 expression but decreased in cells lacking RPS3 expression. RT-qPCR and immunofluorescence assays revealed that CSFV infection inhibited RPS3-mediated antiviral cytokine secretion. Taken together, these findings reveal that RPS3 is a novel antiviral factor that inhibits CSFV proliferation by increasing antiviral cytokine secretion. Keywords: classical swine fever virus; ribosome protein S3; IL-8; INF-β.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 1 1","pages":"55-64"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70877439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Sequencing and phylogenetic characterization of a novel RNA virus in Arma chinensis. 中国犰狳一种新型RNA病毒的测序和系统发育特征。

IF 1.7 4区医学

Acta virologica Pub Date : 2021-01-01 DOI: 10.4149/av_2021_310

Yonghao Dong, Pengjun Xu, Guangwei Ren, Changchun Feng, Dongyang Liu, Lianqiang Jiang, Fangzhao Jia, Changhua Zhang, Qinggang Gao, Yongjie Liu

引用次数: 1