Acta virologica最新文献_第7页

Development of flow cytometry-based Zika virus detection assay. 基于流式细胞术的寨卡病毒检测方法的建立。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_307

Sojan Abraham, Steven Wood

{"title":"Development of flow cytometry-based Zika virus detection assay.","authors":"Sojan Abraham, Steven Wood","doi":"10.4149/av_2022_307","DOIUrl":"https://doi.org/10.4149/av_2022_307","url":null,"abstract":"Standard assays based on ELISA and RT-PCR have been widely used to detect flaviviral infections, including the Zika virus (Zika). Despite their simple, unique, and sensitive features, RT-PCR and ELISA-based assays cannot meet the requirements of high-throughput screening of bulk samples during an outbreak. Several research groups around the world are working on the development of rapid, multiplex, and sensitive assays to overcome the limitations of standard assays used in viral detection. Recent advances in flow cytometry have led to remarkable progress in its use as a basic analysis tool in laboratories. Here, we used the advantages of flow cytometry to develop a Zika detection assay using recombinant Zika envelope (E) protein. The E protein-based flow cytometry assay was able to detect anti-Zika E antibodies from Zika-infected patients, Zika-infected mice, and mice immunized with recombinant Zika E protein. We report the development of the first flow cytometry-based diagnostic assay that can be used for Zika detection. Its rapid turnaround time and ability to detect antibodies from Zika-infected patients can be used to improve the diagnostic accuracy of Zika detection. Keywords: Flavivirus; Zika virus; E protein; NS-1 protein; flow cytometry; ELISA; RT-PCR.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 3","pages":"275-280"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40447097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The induction of virus-neutralizing antibodies in influenza A-infected mice treated with Oseltamivir phosphate: effect of dosage and scheduling. 磷酸奥司他韦在甲型流感感染小鼠体内诱导病毒中和抗体:剂量和时间安排的影响。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_312

Miriam Mikušová, Karolína Tomčíková, Katarína Briestenská, Eva Varečková

{"title":"The induction of virus-neutralizing antibodies in influenza A-infected mice treated with Oseltamivir phosphate: effect of dosage and scheduling.","authors":"Miriam Mikušová, Karolína Tomčíková, Katarína Briestenská, Eva Varečková","doi":"10.4149/av_2022_312","DOIUrl":"https://doi.org/10.4149/av_2022_312","url":null,"abstract":"Oseltamivir phosphate (OS) is currently the most frequently used influenza antiviral drug. It moderates the course of influenza virus type A (IAV) infection, however, its impact on the induction of virus-neutralizing antibodies (VNAbs) is not understood in details. Here, we examined the influence of low (10 mg/kg) or high (60 mg/kg) doses of OS on the viral titer in lungs of BALB/c mice infected with 0.5 LD50 of IAV and on the level of VNAbs. Prophylactic application of OS (6 h before the infection) delayed the increase of viral titer in lungs with a lower peak in comparison to non-treated control mice. After therapeutic OS application (44 h after the infection), maximum of virus titer did not significantly change. However, the induction of VNAbs strongly decreased, to 16.7%-18.1% of the control, after preventive application of high OS dose. A minimal decrease of VNAbs titers was observed in groups of mice treated with low dose of OS applied therapeutically. They lowered to 91.1% / 14 or to 94.1% / 21 days post infection (p.i.) of VNAbs titers of non-treated control mice. In all other groups, levels of VNAbs titers dropped to 26.5-53.7% of those of non-treated mice. It should be noted that VNAbs titers were in direct proportion to maximal virus titers in mouse lungs of corresponding groups. In summary, after OS application the clinical symptoms of the disease were milder or non-observable in all OS-treated groups, but the lowering of VNAbs titers was dependent on the OS dose and interval between drug app-lication and the start of infection. Keywords: influenza A virus; Oseltamivir; prophylactic treatment; therapeutic treatment; virus-neutralizing antibodies.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 3","pages":"281-286"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40447098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

nH115a, a novel inhibitor of the La protein: Effect on expression of multiple RNAs in hepatitis B virus-infected hepatoma cells and embryotoxicity profile. 新型La蛋白抑制剂nH115a:对乙型肝炎病毒感染的肝癌细胞中多种rna表达的影响及胚胎毒性分析

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_108

Jiaqian Pan, Jing Tang

{"title":"nH115a, a novel inhibitor of the La protein: Effect on expression of multiple RNAs in hepatitis B virus-infected hepatoma cells and embryotoxicity profile.","authors":"Jiaqian Pan, Jing Tang","doi":"10.4149/av_2022_108","DOIUrl":"https://doi.org/10.4149/av_2022_108","url":null,"abstract":"The La protein binds to RNA and protects replication of the hepatitis B virus (HBV). We recently developed the compound nH115a, an inhibitor of the La protein that has high stability and anti-HBV activity. However, the mechanism, by which this compound inhibits HBV infection and its safety to embryos, remains unclear. Our goal was to examine the molecular mechanism, by which nH115a inhibits HBV infection, and to characterize its embryotoxicity. Microarray experiments using HepG2. 2. 15 cells (established by transfecting an HBV plasmid into HepG2 hepatoma cells) and bioinformatics analyses were used to measure the effect of nH115a on the expression of lncRNAs, mRNAs, and circRNAs. The embryonic stem cell test was used to assess the embryotoxicity of nH115a. nH115a significantly altered the expression of 2402 lncRNAs, 338 mRNAs, and 559 circRNAs. Gene Ontology (GO) analysis indicated the differentially expressed transcripts functioned in interleukin-2 production, I-SMAD binding, RNA-induced silencing complex (RISC), NLRP3 inflammasome complex assembly, cytoplasmic sequestering of nuclear factor kappa-B (NF-κB), and death receptor binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated the most enriched pathways included transforming growth factor-β (TGF-β) signaling, pathways in cancer, ubiquitin mediated proteolysis, p53 signaling, antigen processing and presentation, Fc gamma R-mediated phagocytosis, and B cell receptor signaling. The results of the embryonic stem cell test indicated that nH115a exhibited weak embryotoxicity. In conclusion, immune responses, TGF-β/SMAD signaling, and cancer-related pathways may function in the nH115a-mediated inhibition of HBV replication. Keywords: hepatitis B virus; La protein; inhibitor; nH115a.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"16 1","pages":"65-76"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70877104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity analysis of grapevine rupestris stem pitting-associated virus from grapevine by colony PCR-SSCP and -RFLP. 利用菌落PCR-SSCP和-RFLP分析葡萄葡萄茎蚀相关病毒的遗传多样性。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_106

G. Hu, Hong-Juan Zhu, Zun-ping Zhang, X. Fan, F. Ren, Ya-feng Dong

{"title":"Genetic diversity analysis of grapevine rupestris stem pitting-associated virus from grapevine by colony PCR-SSCP and -RFLP.","authors":"G. Hu, Hong-Juan Zhu, Zun-ping Zhang, X. Fan, F. Ren, Ya-feng Dong","doi":"10.4149/av_2022_106","DOIUrl":"https://doi.org/10.4149/av_2022_106","url":null,"abstract":"We have developed methods for detecting the genetic diversity of grapevine rupestris stem pitting-associated virus (GRSPaV) based on restriction fragment length polymorphism (RFLP) and single stranded conformational polymorphism (SSCP) in the 905 nt 3' sequence. The amplicons were cloned from six grapevine cultivars, and colony polymerase chain reaction (colony PCR) using recombination bacteria was subsequently analyzed by RFLP and SSCP. Four haplotypes of SSCP and six haplotypes of Sac I RFLPs were defined. The two methods had a 40% discrepancy rate in showing the degree of diversity. All clones were sequenced and were used to construct a phylogenetic tree with seven previously reported GRSPaV sequences. In the tree, all the newly acquired sequences were divided into three clusters, I, II, and III, which corresponded to haplotypes I, II, and III of SSCP, respectively. Haplotype IV of SSCP was grouped into cluster II. A recombination analysis showed that haplotype IV has undergone a recombination event. Together, these results indicate that the SSCP assay is useful for the rapid identification of genetic diversity of GRSPaV. This is the first report of an analysis of the large fragment of GRSPaV by colony PCR-SSCP. Keywords: grapevine; grapevine rupestris stem pitting-associated virus (GRSPaV); RFLP; SSCP; genetic diversity analysis.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 1 1","pages":"85-89"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70877381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Induction of immunogenic response in Balb/c mice by virus-like particles designed using the influenza neuraminidase, hemagglutinin and matrix proteins 用流感神经氨酸酶、血凝素和基质蛋白设计的病毒样颗粒诱导Balb/c小鼠免疫原性反应

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_406

A. Jagadesh, P. P. Mudgal, S. N, J. Mudgal, Anup Jayaram

引用次数: 0

Curcumin inhibits the replication of rotavirus in vitro. 姜黄素在体外抑制轮状病毒复制。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_206

Mina Hassanpour, Abbas Tazarghi, Ali Teimoori, Alijan Tabaraei, Vahid Erfani-Moghadam, Ahad Yamchi, Sedigheh Akhondi, Hadi Razavi Nikoo

引用次数: 0

Common weeds as alternate hosts of Mexican variant of Papaya meleira virus in papaya orchards in México. 普通杂草在墨西哥番木瓜果园中作为番木瓜meleira病毒墨西哥变种的交替寄主。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_209

Isabel García-Cámara, Daisy Pérez-Brito, Raúl Tapia-Tussell, Anuar Magaña-Álvarez, Alberto Cortés-Velázquez, Rodolfo Martín-Mex, Oscar Alberto Moreno-Valenzuela

{"title":"Common weeds as alternate hosts of Mexican variant of Papaya meleira virus in papaya orchards in México.","authors":"Isabel García-Cámara, Daisy Pérez-Brito, Raúl Tapia-Tussell, Anuar Magaña-Álvarez, Alberto Cortés-Velázquez, Rodolfo Martín-Mex, Oscar Alberto Moreno-Valenzuela","doi":"10.4149/av_2022_209","DOIUrl":"https://doi.org/10.4149/av_2022_209","url":null,"abstract":"Presence of alternate hosts of plants is a great threat to the agriculture industry. Plants from several species growing in the papaya orchards affected by papaya sticky disease were examined for Papaya meleira virus (PMeV) infection causing this disease. The viral dsRNA was already detected in some plants from the family Poaceae or in watermelon. To identify new hosts of PMeV, we have collected 38 plant species belonging to 15 families of common weed species found in papaya-growing areas in México and used reverse-transcription PCR (RT-PCR) or quantitative real-time RT-PCR (RT-qPCR) for virus detection. We have detected the viral RNA in 11 species belonging to the families Acanthaceae, Fabaceae and Poaceae. Under experimental conditions, PMeV-Mx in Panicum hirsutum and Ruellia nudiflora inoculated weed species, showed that PMeV-Mx is able to replicate in plant cells of these species and spread in a systemic way. These results highlight the importance of weed species as potential virus reservoirs for PMeV-Mx Keywords: Papaya meleira virus; papaya sticky disease; Carica papaya; RT-PCR; TaqMan.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 2","pages":"192-194"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40408237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Quil-A, E. coli DNA and Montanide™ ISA 206 adjuvant combination on the antibody response to foot-and-mouth disease vaccine in sheep. quila、E. coli DNA和Montanide™ISA 206佐剂组合对羊口蹄疫疫苗抗体应答的评价

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_304

Can Çokçalişkan, Tunçer Türkoğlu, Beyhan Sareyyüpoğlu, Pelin Tuncer-Göktuna, Banu Bayri Özbilge, Ergün Uzunlu, Ayça Kürkçü, Eylem Aras Uzun, Veli Gülyaz

{"title":"Evaluation of Quil-A, E. coli DNA and Montanide™ ISA 206 adjuvant combination on the antibody response to foot-and-mouth disease vaccine in sheep.","authors":"Can Çokçalişkan, Tunçer Türkoğlu, Beyhan Sareyyüpoğlu, Pelin Tuncer-Göktuna, Banu Bayri Özbilge, Ergün Uzunlu, Ayça Kürkçü, Eylem Aras Uzun, Veli Gülyaz","doi":"10.4149/av_2022_304","DOIUrl":"https://doi.org/10.4149/av_2022_304","url":null,"abstract":"Vaccination is one of the basic strategies in the fight against foot-and-mouth disease (FMD) in endemic regions. Today, commercially available FMD vaccines are prepared with inactive whole virion, which has low immunogenicity. Therefore, considerable effort has been devoted to finding novel adjuvants. Although mineral oils are among the most common adjuvants, it is still difficult to provide a long-term and robust immune response. Combined adjuvant systems are currently being studied to solve the problem. Saponins and CpG-ODNs have been shown to increase the immune response to vaccines individually in various studies. In this study, the effect of different adjuvants and their combinations (Quil-A, E. coli DNA, and MontanideTM ISA 206) on total and neutralizing antibody response in sheep was investigated. According to the results, the Quil-A group induced the highest antibody level, followed by the combination of Quil-A and the E. coli DNA group. The group containing E. coli DNA also caused a higher antibody response than the group containing only MontanideTM ISA 206 for certain days of sampling. These affordable alternatives of saponin and CpG sources can be used individually to increase the potency of the FMD vaccine for mass vaccinations of sheep. Keywords: foot-and-mouth disease; vaccine; adjuvant; Quil-A; E. coli DNA; combination of adjuvants.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 3","pages":"197-205"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40424842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Down regulation of defensin genes during SARS-CoV-2 infection. SARS-CoV-2感染过程中防御素基因的下调

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_306

Mohammed M Idris, Sarena Banu, Archana B Siva, Ramakrishnan Nagaraj

{"title":"Down regulation of defensin genes during SARS-CoV-2 infection.","authors":"Mohammed M Idris, Sarena Banu, Archana B Siva, Ramakrishnan Nagaraj","doi":"10.4149/av_2022_306","DOIUrl":"https://doi.org/10.4149/av_2022_306","url":null,"abstract":"Defensins, crucial components of the innate immune system, play a vital role against infection as part of frontline immunity. Association of SARS-CoV-2 infection with defensins has not been investigated. In this study, we have investigated the expression of defensin genes in the buccal cavity from patients with COVID-19 infection along with negative control samples. Nasopharyngeal/oropharyngeal swab samples collected for screening SARS-CoV-2 infection in early 2020 from Hyderabad, India, were analyzed for the expression of major defensin genes by the quantitative real-time reverse transcription polymerase chain reaction, qRT-PCR. Forty SARS-CoV-2 infected positive and 40 negative swab samples were selected for this study. Based on the qRT-PCR analysis involving gene specific primers for defensin genes, 9 defensin genes were found to be expressed in the nasopharyngeal/oropharyngeal cavity. Four defensin genes were found to be significantly down regulated in SARS-CoV-2 infected patients in comparison with the control samples based on differential expression analysis. The significantly down regulated genes were defensin beta 4A/B, 106B, 107B, and 103A. Down regulation of human beta defensin 2, 3, 6 and 7 suggests that antiviral innate immune response provided by defensins may be compromised in SARS-CoV-2 infection resulting in progression of the disease. Correction of the down regulation process through appropriate defensin peptide-based therapy could be an attractive method of treatment. Keywords: host defense; defensins; COVID-19; gene regulation; SARS-CoV-2.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 3","pages":"249-253"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40447094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Generation and characterization of interferon-beta-resistant H1N1 influenza A virus. 干扰素- β耐药甲型H1N1流感病毒的产生和特性

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_311

Brady T Hickerson, Simone E Adams, Nicolai V Bovin, Raymond P Donnelly, Natalia A Ilyushina

{"title":"Generation and characterization of interferon-beta-resistant H1N1 influenza A virus.","authors":"Brady T Hickerson, Simone E Adams, Nicolai V Bovin, Raymond P Donnelly, Natalia A Ilyushina","doi":"10.4149/av_2022_311","DOIUrl":"https://doi.org/10.4149/av_2022_311","url":null,"abstract":"Interferons (IFNs) mediate innate antiviral activity against many types of viruses, including influenza viruses. In light of their potential use as anti-influenza agents, we examined whether resistance to these host antiviral proteins can develop. We generated IFN-β-resistant variants of the A/California/04/09 (H1N1) virus by serial passage in a human airway epithelial cell line, Calu-3, under IFN-β selective pressure. The combination of specific mutations (i.e., L373I in PB1, K154E1, D222G1, I56V2, and V122I2 in HA, and M269I in NA) correlated with decreased ability of the virus to induce expression of IFN (IFNB1, IFNL1, and IFNL2/3) and IFN-stimulated genes (IFIT1, IFIT3, OAS1, IRF7, and MX1) by target respiratory epithelial cells. In addition, the IFN-induced mutations were associated with decreased HA binding affinity to α2,6 sialyl receptors, reduced NA enzyme catalytic activity, and decreased polymerase transcription activity. Our findings demonstrate that the mutations in the influenza HA, NA, and PB1 proteins induced by IFN-b selective pressure significantly increase viral ability to productively infect and replicate in host cells. Keywords: influenza A virus; interferon-β; lung epithelial cells; interferon response.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 3","pages":"263-274"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40447096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0