Acta virologica最新文献

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Insights into the factors affecting synonymous codon usage bias in the coat protein of soil-borne sugar beet-infecting viruses 土传甜菜侵染病毒外壳蛋白同义密码子使用偏向性影响因素的研究
IF 1.7 4区 医学
Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_402
S. Farzadfar, R. Pourrahim
{"title":"Insights into the factors affecting synonymous codon usage bias in the coat protein of soil-borne sugar beet-infecting viruses","authors":"S. Farzadfar, R. Pourrahim","doi":"10.4149/av_2022_402","DOIUrl":"https://doi.org/10.4149/av_2022_402","url":null,"abstract":"","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"40 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70877080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interaction between proteins of the PPARγ and NFκB immune response pathways and rotavirus non-structural proteins. PPARγ和NFκB免疫反应通路蛋白与轮状病毒非结构蛋白的相互作用。
IF 1.7 4区 医学
Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_105
Dory Gómez, C. Guerrero
{"title":"Interaction between proteins of the PPARγ and NFκB immune response pathways and rotavirus non-structural proteins.","authors":"Dory Gómez, C. Guerrero","doi":"10.4149/av_2022_105","DOIUrl":"https://doi.org/10.4149/av_2022_105","url":null,"abstract":"Cells infected with MA104 rotavirus and/or transfected with plasmids expressing NSP proteins, were analyzed for expression of cellular proteins related to NFκB and PPARγ pathways and evaluated through the ELISA, luminescence, flow cytometry and Western blot techniques. The association between cellular and viral (NSPs) proteins was examined by ELISA, epifluorescence and confocal microscopy techniques. It was observed that NSP1 protein interacts with RXR, NSP1, and NSP3 with PPARγ, NSP2 with p-IKKα/β and NSP5 with NFκB proteins. We have found that phosphorylated PPARγ is localized in cytoplasm and transcriptional activity of PPRE is diminished. These results lead to the conclusion, that RRV activates the proinflammatory pathway, increasing the expression of NFκB and possibly by PPARγ phosphorylation, its translocation to the nucleus is impeded, thus inactivating the proinflammatory pathway. Keywords: rotavirus; PPARγ; NFκB; NSPs; RRV.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 1 1","pages":"39-54"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70877483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A gene expression signature that correlates with CD8+T cell expansion in acute Epstein-Barr virus infection. 急性eb病毒感染中与CD8+T细胞扩增相关的基因表达特征
IF 1.7 4区 医学
Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_303
Yijing Chen, Hanqing Wang, Xia Liu, Bing Luo
{"title":"A gene expression signature that correlates with CD8+T cell expansion in acute Epstein-Barr virus infection.","authors":"Yijing Chen,&nbsp;Hanqing Wang,&nbsp;Xia Liu,&nbsp;Bing Luo","doi":"10.4149/av_2022_303","DOIUrl":"https://doi.org/10.4149/av_2022_303","url":null,"abstract":"<p><p>Acute infectious mononucleosis (AIM) is associated with Epstein-Barr virus (EBV) infection. We explored molecular mechanisms regarding the expression of CD8+T cells in convalescence stage (CONV). Differentially expressed genes (DEGs) were identified by analyzing GEO expression profiles. Subsequently, Gene Set Enrichment Analysis (GSEA), Protein-Protein Interactions (PPI) network, and gene-micro RNAs networks were used to identify hub genes and associated pathways. GSEA provided evidence that the top 3 gene sets in GSEA were all related to integrins. We identified ten hub genes in the PPI network and DGIdb was applied to predict potential targets that might reverse the expression of hub genes. Our study enhances a mechanistic understanding of the CD8+T cells expansion in acute EBV infection and provides potential treatment targets for further research. Keywords: acute infectious mononucleosis; bioinformatics; CD8+T cells; differentially expressed genes; EBV.</p>","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 3","pages":"216-227"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40424847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genomic profiling of three progenies isolated from a swarm of eGFP-tagged canine distemper viruses undergoing 40 serial passages 从一群egfp标记的犬瘟热病毒中分离出的三个子代进行40次连续传代的基因组分析
IF 1.7 4区 医学
Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_408
Shuning Zhou, Ling Wang, Jiahui Lin, Ning Wang, Yongle Yu, Youming Zhang, Fuxiao Liu
{"title":"Genomic profiling of three progenies isolated from a swarm of eGFP-tagged canine distemper viruses undergoing 40 serial passages","authors":"Shuning Zhou, Ling Wang, Jiahui Lin, Ning Wang, Yongle Yu, Youming Zhang, Fuxiao Liu","doi":"10.4149/av_2022_408","DOIUrl":"https://doi.org/10.4149/av_2022_408","url":null,"abstract":"","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"1 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70877723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Simultaneous detection of velogenic Newcastle disease virus of genotype XIII 2.2 from spot-billed pelican and backyard chicken: implications to the viral maintenance and spread. 在斑嘴鹈鹕和家鸡中同时检测到基因型为XIII 2.2的新城疫病毒:对病毒维持和传播的意义。
IF 1.7 4区 医学
Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_305
Deepthi Balam, Ratnamma Doddamane, Ramani Pushpa Rayudu, Shrikrishna Isloor, Veeregowda Belamaranahally, Himaja Maddireddy, Muralidhar Metta
{"title":"Simultaneous detection of velogenic Newcastle disease virus of genotype XIII 2.2 from spot-billed pelican and backyard chicken: implications to the viral maintenance and spread.","authors":"Deepthi Balam,&nbsp;Ratnamma Doddamane,&nbsp;Ramani Pushpa Rayudu,&nbsp;Shrikrishna Isloor,&nbsp;Veeregowda Belamaranahally,&nbsp;Himaja Maddireddy,&nbsp;Muralidhar Metta","doi":"10.4149/av_2022_305","DOIUrl":"https://doi.org/10.4149/av_2022_305","url":null,"abstract":"<p><p>Despite the widespread occurence of Newcastle disease virus (NDV) in different avian species, there has been scanty reports on genetic characterization of NDV strains from wild birds in India. During 2017-18, a total of forty eight cloacal swab samples were collected from apparently healthy migratory birds (painted storks, n = 32 and spot-billed pelicans, n = 16) at the Telineelapuram bird sanctuary of Andhra Pradesh, India. NDV was isolated from a spot-billed pelican (NDV/Pelican/Telineelapuram/2018) which is genetically identical to that isolated from a naturally infected backyard chicken flock (NDV/Chicken/SKLM-1/2018). The isolates are found to be velogenic based on mean death time, intracerebral pathogenicity index and the putative fusion protein cleavage site (112R-R-R-K-R-F117). Phylogenetic analysis based on full-length fusion gene classified the isolates into genotype XIII, sub-genotype 2.2, however these isolates demonstrated multiple amino acid substitutions in the critical domains of F and HN proteins. The pelican strain (MIG-9) was tested for its pathogenic and transmission potential in three-weeks-old broiler chickens and the isolate proved to be highly virulent to chickens. To the best of our knowledge, this is the first evidence for the role of spot-billed pelicans in the maintenance of virulent NDV and its transmission to chickens in India. This study further highlights the role of wild birds in NDV transmission and the need for enhanced biosecurity in commercial poultry operations. Keywords: Newcastle disease virus; Pelecanus philippensis; chicken; transmission; pathogenicity; India.</p>","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 3","pages":"238-248"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40447093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Molecular identification and characterization of badnaviruses infecting sugarcane in Ethiopia. 侵染埃塞俄比亚甘蔗的恶性病毒的分子鉴定和鉴定。
IF 1.7 4区 医学
Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_101
Simatsidk Haregu, D. Kidanemariam, A. Abraham
{"title":"Molecular identification and characterization of badnaviruses infecting sugarcane in Ethiopia.","authors":"Simatsidk Haregu, D. Kidanemariam, A. Abraham","doi":"10.4149/av_2022_101","DOIUrl":"https://doi.org/10.4149/av_2022_101","url":null,"abstract":"Sugarcane bacilliform virus (SCBV) is an economically important virus limiting sugarcane production worldwide. Although Ethiopia is a major sugarcane producer, and virus-like symptoms are frequently observed in sugarcane fields, there is a complete lack of information as to the occurrence, distribution and molecular properties of SCBV. This study was aimed to identify and characterize SCBV isolates in Ethiopia using molecular methods. Out of 292 leaf samples collected and tested by PCR, 76 samples (26% incidence level) were found SCBV-positive. Nucleotide sequence analysis results showed that three Ethiopian isolates (SCBV-EtS3, SCBV-EtS6 and SCBV-EtC10) shared high level of nucleotide identity (99.5-100%) among themselves and with SCBV isolates from China (accession numbers MH037614 and MH037915). Another isolate, SCBV-EtC2, shared maximum identity of 78% with the other three SCBV isolates from Ethiopia and 99.8% with SCBV isolates from China (KM214357 and KM214307). Based on phylogenetic analysis, isolates from Ethiopia were segregated into two different clusters. Isolates SCBV-EtS3, SCBV-EtS6 and SCBV-EtC10 clustered with SCBV-Q group and SCBV-EtC2 with SCBV-H group. This study provides information on the occurrence of SCBV for the first time in Ethiopia and also contributes to the understanding of the genetic diversity of SCBV. Keywords: Caulimoviridae; RNase H, Saccharum spp.; Sugarcane bacilliform virus.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 1 1","pages":"3-10"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70877031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Transcriptomic analyses reveal highly conserved plant amalgavirus genomes in different species of Allium. 转录组学分析显示葱属植物中汞齐病毒基因组高度保守。
IF 1.7 4区 医学
Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_102
J. A. D. dos Santos, A. M. F. Silva, Júlio Carlos Polimeni de Mesquita, R. Blawid
{"title":"Transcriptomic analyses reveal highly conserved plant amalgavirus genomes in different species of Allium.","authors":"J. A. D. dos Santos, A. M. F. Silva, Júlio Carlos Polimeni de Mesquita, R. Blawid","doi":"10.4149/av_2022_102","DOIUrl":"https://doi.org/10.4149/av_2022_102","url":null,"abstract":"This work aims to study amalgavirus diversity in different species of allium collected around the world. Transcriptomic data of 19 Sequence Read Archive runs available at GenBank, as well as RNA-seq data generated from onion tissue from fields in Brazil were used to assemble nine allium cepa amalgavirus 1 (AcAV-1) and nine allium cepa amalgavirus 2 (AcAV-2) genomes from different species of allium worldwide. Sequence demarcation tool analyses of RdRp amino acid sequences revealed identities above 99% within each species, except for an isolate of AcAV-1 from Allium escalonicum from China. This work contributes to the understanding of the genetic diversity of amalgaviruses that infect the genus Allium. Keywords: amalgaviruses; Allium transcriptomic datasets; Allium sp.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 1 1","pages":"11-17"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70877037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Integrase deletion mutation impairs the Gag cleavage in prototype foamy virus 整合酶缺失突变影响泡沫病毒原型Gag的裂解
IF 1.7 4区 医学
Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_401
Jinsun Kim, C. Shin
{"title":"Integrase deletion mutation impairs the Gag cleavage in prototype foamy virus","authors":"Jinsun Kim, C. Shin","doi":"10.4149/av_2022_401","DOIUrl":"https://doi.org/10.4149/av_2022_401","url":null,"abstract":"","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"1 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70877403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Artemisia capillaris nucleorhabdovirus 1, a novel member of the genus Alphanucleorhabdovirus, identified in the Artemisia capillaris transcriptome. 毛蒿核habdovirus 1是毛蒿核habdovirus属的一个新成员。
IF 1.7 4区 医学
Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_204
Dongjin Choi, Chaerim Shin, Ken Shirasu, Yasunori Ichihashi, Yoonsoo Hahn
{"title":"Artemisia capillaris nucleorhabdovirus 1, a novel member of the genus Alphanucleorhabdovirus, identified in the Artemisia capillaris transcriptome.","authors":"Dongjin Choi,&nbsp;Chaerim Shin,&nbsp;Ken Shirasu,&nbsp;Yasunori Ichihashi,&nbsp;Yoonsoo Hahn","doi":"10.4149/av_2022_204","DOIUrl":"https://doi.org/10.4149/av_2022_204","url":null,"abstract":"<p><p>A novel, negative-sense, single-stranded RNA virus, Artemisia capillaris nucleorhabdovirus 1 (AcNRV1), was identified in the transcriptome data of Artemisia capillaris (commonly known as capillary wormwood) root tissue. The AcNRV1 genome contains six open reading frames encoding a nucleocapsid (N), phosphoprotein, movement protein P3, matrix protein, glycoprotein, and polymerase (L). Sequence comparison and phylogenetic analysis using L and N protein sequences revealed that AcNRV1 is a novel member of the genus Alphanucleorhabdovirus, one of the six plant-infecting rhabdovirus genera of the family Rhabdoviridae. Wheat yellow striate virus and rice yellow stunt virus were identified as the closest known rhabdoviruses of AcNRV1. The conserved regulatory sequences involved in transcription termination/polyadenylation (TTP) and transcription initiation (TI) of individual genes were identified in the AcNRV1 genome with the consensus sequence 3'-(A/U)UUAUUUUU-GGG-UUG-5' (in the negative-sense genome), whereby dashes separate the TTP, untranscribed intergenic spacer, and TI elements. The AcNRV1 genome sequence will contribute to further understanding the genome structural evolution of plant rhabdoviruses. Keywords: Artemisia capillaris nucleorhabdovirus 1; plant virus; Alphanucleorhabdovirus; Rhabdoviridae.</p>","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 2","pages":"149-156"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40408803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Molecular characterization of a ssRNA mycovirus isolated from the forest pathogenic fungus Armillaria ostoyae. 从森林致病真菌蜜环菌分离的ssRNA分枝病毒的分子特征。
IF 1.7 4区 医学
Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_309
Tomáš Tonka, Lucie Walterová, Ondřej Hejna, Vladislav Čurn
{"title":"Molecular characterization of a ssRNA mycovirus isolated from the forest pathogenic fungus Armillaria ostoyae.","authors":"Tomáš Tonka,&nbsp;Lucie Walterová,&nbsp;Ondřej Hejna,&nbsp;Vladislav Čurn","doi":"10.4149/av_2022_309","DOIUrl":"https://doi.org/10.4149/av_2022_309","url":null,"abstract":"<p><p>Letter to the editor (no abstract) Keywords.</p>","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 3","pages":"290-294"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40424845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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