Acta virologica最新文献_第5页

Genome sequence of a Papaya ringspot virus from khejri (Prosopis cineraria) transcriptome from India 来自印度番木瓜环斑病毒转录组的基因组序列

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_409

M. Prajapati, Jitender Singh, Pankaj Kumar, V. Baranwal

引用次数: 0

Natural infection of cape gooseberry (Physalis peruviana) by the potyvirus Tamarillo leaf malformation virus (TaLMV). 柽柳叶畸形病毒(TaLMV)对猕猴桃自然侵染的研究。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_211

Erika Corrales-Cabra, Andrea Restrepo Escobar, Mónica Higuita Valencia, Yuliana Gallo García, Mauricio Marín Montoya, Pablo Gutiérrez Sánchez

{"title":"Natural infection of cape gooseberry (Physalis peruviana) by the potyvirus Tamarillo leaf malformation virus (TaLMV).","authors":"Erika Corrales-Cabra, Andrea Restrepo Escobar, Mónica Higuita Valencia, Yuliana Gallo García, Mauricio Marín Montoya, Pablo Gutiérrez Sánchez","doi":"10.4149/av_2022_211","DOIUrl":"https://doi.org/10.4149/av_2022_211","url":null,"abstract":"Tamarillo leaf malformation virus (TaLMV) is a potyvirus first discovered in cape gooseberry fields in Eastern, and South-western Antioquia. This virus is responsible for a very damaging disease that has resulted in significant reductions in yields and cultivated area for this crop in Colombia. Tamarillo is frequently co-cultivated with other solanaceous plants but no evidence for cross-pathogenicity of TaLMV has been found until now. In this work, we report a natural infection of cape gooseberry (Physalis peruviana L.) by TaLMV. Infection by TaLMV was detected by RNAseq screening of cape gooseberry fields and confirmed by RT-qPCR and Sanger sequencing. The sequenced genome is 99.3% identical to previously sequenced TaLMV isolates, and evidence suggests that it can accumulate at high loads in this new reported host. RT-qPCR analysis indicates that TaLMV is already widely distributed, can naturally infect other solanaceous hosts and may become an emerging threat to the cape gooseberry agroindustry, the second most important exotic fruit export in Colombia. Keywords: high-throughput sequencing; plant virology; Potyviridae; RT-qPCR; Solanaceae.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 2","pages":"174-181"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40408234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Selective human cells in vitro transduction with porcine endogenous retrovirus (PERV). 猪内源性逆转录病毒(PERV)体外转染人细胞的选择性研究。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_201

Sabina Gałka, Ewa Nowak, Ilona Bednarek

{"title":"Selective human cells in vitro transduction with porcine endogenous retrovirus (PERV).","authors":"Sabina Gałka, Ewa Nowak, Ilona Bednarek","doi":"10.4149/av_2022_201","DOIUrl":"https://doi.org/10.4149/av_2022_201","url":null,"abstract":"Porcine xenograft transplantation raises concerns in humans about the risk of infection with porcine endogenous retroviruses (PERV) as they are an integral part of the pig genome and are therefore very difficult to exclude. In this study, for the first time, a relationship between the provirus genes sequences and released virions from pig cell line and the embedded sequence of this retrovirus in infected human cells was analyzed. PERV infection of human cells HEK-293 and HeLa and detection of PERV in pig PK-15 cells and supernatant were assessed by QPCR or RT-QPCR using primers specific for envA, envB, gag, pol genes and LTR region. Sequence analysis was performed at the DNA level and changes in the amino acid sequence were deduced in silico. Fifty nucleotide substitutions (45 in pol, 3 in gag and one each in envA and envB) were detected and most of these were heterozygous (42), which were present mainly in PK-15 cells. Our results show that sequence of the pol gene and the Pol protein is less conserved compared to the other PERV genes and PERV with some polymorphisms were not released from pig cells or/and do not infect human cells. PERV virions with a homozygous allele system were released from PK-15 cells, although their sequence replicated on the basis of the heterozygous PERV provirus sequence in PK-15. The newly discovered selective transduction of human cells with PERV will be helpful in studying the characteristics and genetic variability of the retrovirus genes to ensure safe xenotransplantation. Keywords: PERV; porcine endogenous retroviruses; infection; genetic polymorphism; xenotransplantation.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 2","pages":"110-126"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40408800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First record of mosquito-borne Sindbis virus (genotype I) in the Czech Republic. 捷克共和国首次记录蚊媒信德比斯病毒(基因型I)。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_313

Silvie Šikutová, Jan Mendel, Jakub Vojtíšek, Zdeněk Hubálek, Ivo Rudolf

引用次数: 0

RT-qPCR based determination of SARS-CoV-2 in health care workers, Cologne, Germany – A pre-delta/pre-omicron proof of principle field study 基于RT-qPCR的SARS-CoV-2在德国科隆卫生保健工作者中的检测——一项delta前/组粒前的原理现场研究证明

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_403

Gina Spölgen, J. Lüsebrink, V. Schildgen, O. Schildgen

引用次数: 0

A novel RNA virus, Thesium chinense closterovirus 1, identified by high-throughput RNA-sequencing of the parasitic plant Thesium chinense. 利用高通量RNA测序技术鉴定出一种新的中国Thesium clostervirus 1。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_302

Chaerim Shin, Dongjin Choi, Ken Shirasu, Yasunori Ichihashi, Yoonsoo Hahn

{"title":"A novel RNA virus, Thesium chinense closterovirus 1, identified by high-throughput RNA-sequencing of the parasitic plant Thesium chinense.","authors":"Chaerim Shin, Dongjin Choi, Ken Shirasu, Yasunori Ichihashi, Yoonsoo Hahn","doi":"10.4149/av_2022_302","DOIUrl":"https://doi.org/10.4149/av_2022_302","url":null,"abstract":"The genome sequence of a closterovirus (genus Closterovirus, family Closteroviridae), tentatively named Thesium chinense closterovirus 1 (TcCV1), was identified by performing high-throughput RNA-sequencing of the haustoria and root tissues of Thesium chinense, a parasitic plant. The TcCV1 genome was predicted to encode nine proteins, eight of which have orthologs in previously identified closteroviruses. The TcCV1 RNA-dependent RNA polymerase (RdRp) and heat shock protein 70 homolog (Hsp70h) showed 27.8-68.2% and 23.8-55.1% amino acid identity, respectively, to orthologous proteins of known closteroviruses. The putative +1 ribosomal frameshifting site required for producing RdRp was identified as GUUUAGC with UAG stop codon and the skipped nucleotide U. Phylogenetic trees based on RdRp and Hsp70h show that TcCV1 is a novel member of the genus Closterovirus, forming a subclade with a group of known closteroviruses, including mint virus 1 and carnation necrotic fleck virus. The genome sequence of TcCV1 may be useful for studying the genome evolution of closteroviruses. Keywords: Thesium chinense closterovirus 1; Closterovirus; Closteroviridae; Thesium chinense.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 3","pages":"206-215"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40424843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Acetylation of late expression factor 4 is crucial for the transcription and proliferation of Bombyx mori nucleopolyhedrovirus. 晚期表达因子4的乙酰化对家蚕核多角体病毒的转录和增殖至关重要。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_109

Jonas Ngowo, Xu Gao, Yajie Zhu, Fuxiang Mao, Jihai Lei, E. Obeng, Wei Yu

{"title":"Acetylation of late expression factor 4 is crucial for the transcription and proliferation of Bombyx mori nucleopolyhedrovirus.","authors":"Jonas Ngowo, Xu Gao, Yajie Zhu, Fuxiang Mao, Jihai Lei, E. Obeng, Wei Yu","doi":"10.4149/av_2022_109","DOIUrl":"https://doi.org/10.4149/av_2022_109","url":null,"abstract":"Late expression factor 4 (LEF4), RNA polymerase subunit of Bombyx mori nucleopolyhedrovirus (BmNPV), plays an enzymatic role to enhance the capping of pre-mRNA of late and very late genes. Lysine acetylation is a post-translational modification process having many important functions associated with the regulation of a gene expression. Our previous study on lysine acetylome in BmNPV infected BmN cells showed that LEF 4 was acetylated at lysine 76 (K76). However, it is still unclear whether the modification of K76 residue contributes to the modulation of viral gene transcription. To elucidate the role played by acetylation or deacetylation of LEF4 K76 in the transcription of viral genes, we constructed acetylation mimicking and deacetylation mimicking mutant virus, K76Q and K76R, respectively. We then transfected BmN cells with these mutants and analyzed the level of pre-mRNA at different times. The K76R showed a significant decrease in the mRNA transcription level of vp39 and p10 genes at 48 and 72 h post-transfection, while K76Q did not show any significant changes compared with lef4-Wt. We further detected the virus titer of lef4-Wt, K76Q [et] K76R, and it was found that K76R impaired the virus infectivity ability at 72 and 96 h, while K76Q did not affect the virus infectivity. Moreover, the yeast two hybrid technique (Y2H) showed that both mutants (K76Q [et] K76R) affected the association of LEF 4 with the P47 protein. Taken together, these results indicated that acetylation modification of K76 is important for the proper transcription of late and very late genes, and the effectiveness of viral infection. Keywords: BmNPV lef4 gene; lysine acetylation, late genes transcription; BmNPV p47 gene; infectivity.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 1 1","pages":"77-84"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70877220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suitability of different plant species for experimental agroinfection with Plum pox virus-based expression vector for potential production of edible vaccines. 以梅痘病毒为基础的表达载体对不同植物侵染试验的适宜性及可食用疫苗的潜在生产。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_111

Adam Achs, M. Glasa, P. Alaxin, Z. Šubr

引用次数: 1

Isolation and characterization of novel Spodoptera exigua nucleopolyhedrovirus strains in Turkey and their potential for use in biological control 土耳其新夜蛾核多角体病毒株的分离、鉴定及其生物防治潜力

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_407

D. Gencer, Z. Bayramoğlu, Çetin Mutlu, I. Demir

引用次数: 0

Genomic phylogenetic analyses of four major hand, foot and mouth disease-related enteroviruses. 四种主要手足口病相关肠道病毒的基因组系统发育分析。

IF 1.7 4区医学

Acta virologica Pub Date : 2022-01-01 DOI: 10.4149/av_2022_104

Yajuan Dong, Zhenzhou Wan, Shenwei Li, Jian-Hua Wang, Xia Jin, Guoying Yu, Chiyu Zhang

{"title":"Genomic phylogenetic analyses of four major hand, foot and mouth disease-related enteroviruses.","authors":"Yajuan Dong, Zhenzhou Wan, Shenwei Li, Jian-Hua Wang, Xia Jin, Guoying Yu, Chiyu Zhang","doi":"10.4149/av_2022_104","DOIUrl":"https://doi.org/10.4149/av_2022_104","url":null,"abstract":"Enteroviruses had diverged into many types, some of which cause hand, foot and mouth disease (HFMD) in children. The predominant enterovirus types associated with HFMD are EVA71, CVA16, CVA6 and CVA10. Four enterovirus types were classified into subtypes based on VP1 sequences. However, the phylogenetics of these enteroviruses is rarely concerned at the genomic level. In this study, we performed the phylogenetic analyses of the EVA71, CVA16, CVA6 and CVA10 using available full-length genomic sequences. We found that the topologies of phylogenetic trees of full-length genomic sequences and VP1 sequences were almost consistent, except few subtypes of EVA71 and CVA10. The mean genetic divergence was 15.8-27% between subtypes and less than 12% within subtypes/sub-subtypes at genomic level. Comparison of phylogenetic topologies between genomic and VP1 sequences helped us to identify two new EVA71 inter-subtype recombinants RF01_CC4 and RF02_CC4. Furthermore, EVA71 subtypes C1 and C2 and CVA10 subtype D were found to originate through inter-subtype recombination. The genomic reference sequences of these enteroviruses are provided here for subtyping. The results provide important insights into the understanding of the evolution and epidemiology of the four enteroviruses. Keywords: enterovirus; hand; foot and mouth disease; classification; genetic distance; recombination.","PeriodicalId":7205,"journal":{"name":"Acta virologica","volume":"66 1 1","pages":"27-38"},"PeriodicalIF":1.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70876985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0