以梅痘病毒为基础的表达载体对不同植物侵染试验的适宜性及可食用疫苗的潜在生产。

IF 1.1 4区医学 Q4 VIROLOGY

Acta virologica Pub Date : 2022-01-01 DOI:10.4149/av_2022_111

Adam Achs, M. Glasa, P. Alaxin, Z. Šubr

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引用次数: 1

摘要

采用农杆菌介导的方法，检测了9种草本植物对李痘病毒(PPV)的易感性。其中两种感染，即菠菜(局部感染)和油籽罂粟(全身感染)。作为对照，PPV侵染在灌浆后成功地在李树幼苗中建立，首次报道了李树属植物的土壤侵染。根据我们的研究结果，油籽罂粟可以被认为是ppv衍生表达载体生产可食用疫苗的候选宿主。关键词:agroinfiltration;病毒主机;罂粟花;菠菜。

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Suitability of different plant species for experimental agroinfection with Plum pox virus-based expression vector for potential production of edible vaccines.

Nine herbaceous plant species were tested for susceptibility to Plum pox virus (PPV) by Agrobacterium-mediated delivery of its infectious cDNA clone. Two of them became infected, namely spinach (local infection) and oilseed poppy (systemic infection). As a control, PPV infection was successfully established in plum seedlings following agroinfiltration, thus providing the first report of agroinfection in Prunus species. According to our results, oilseed poppy can be considered as a candidate host for the production of edible vaccines by a PPV-derived expression vector. Keywords: agroinfiltration; virus host; poppy; spinach.

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来源期刊

Acta virologica 医学-病毒学

CiteScore

3.10

自引率

11.80%

发文量

审稿时长

>12 weeks

期刊介绍： Acta virologica is an international journal of predominantly molecular and cellular virology. Acta virologica aims to publish papers reporting original results of fundamental and applied research mainly on human, animal and plant viruses at cellular and molecular level. As a matter of tradition, also rickettsiae are included. Areas of interest are virus structure and morphology, molecular biology of virus-cell interactions, molecular genetics of viruses, pathogenesis of viral diseases, viral immunology, vaccines, antiviral drugs and viral diagnostics.