Identification of dicistro-like viruses in the transcriptome data of Striga asiatica and other plants.

Abstract

Dicistroviruses (the family Dicistroviridae) are positive-sense single-stranded RNA viruses of the order Picornavirales, which is a rapidly growing viral group. They have been detected in a wide range of animals, predominantly in insects and crustaceans. In this study, we identified the genome sequences of 14 dicistro-like viruses in the transcriptome data from 12 plant species, including Striga asiatica dicistro-like virus 1 and 2 identified in the transcriptome data of Striga asiatica. Sequence comparison and phylogenetic analysis indicated that these 14 plant-associated dicistro-like viruses were novel members of the family Dicistroviridae, five of which are placed within the genera Aparavirus and Cripavirus, which mainly consist of viruses infecting animals, including insects. The other nine plant dicistro-like viruses formed clades with unclassified dicistroviruses. Our study implies that a wide range of plant species may serve as hosts for dicistroviruses or reservoirs for their transmission. Keywords: dicistrovirus; Dicistroviridae; plant; transcriptome; Striga asiatica.