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引用次数: 1
摘要
采用RNA测序和cDNA末端快速扩增的方法，确定了一种新型中华剑虫病毒的全基因组。该病毒具有单链RNA基因组，包含10,540个核苷酸(nt)，不包括聚(a)尾部。在意义方向上预测了两个不重叠的开放阅读框(ORF):一个位于基因组5'端(6,219 nt)的长ORF编码2,072个氨基酸(aa)的多肽，一个位于基因组3'端(3,033 nt)的短ORF编码1,010个氨基酸的多肽。系统发育分析表明，该病毒聚集在一大群目前尚未确定的小核糖核酸样病毒中，具有很高的自举值。我们将该病毒分离物命名为中华袖珍小核糖核酸样病毒1号(AcPV-1)。野生中华armma Arma chinensis AcPV-1感染率较低(5.48%，146份标本中8份呈阳性)。关键词:中华犰狳;基因组特征;系统发育分析;中华袖珍猴类病毒1型;患病率。
Sequencing and phylogenetic characterization of a novel RNA virus in Arma chinensis.
The complete genome of a novel virus from Arma chinensis was determined by RNA sequencing and rapid amplification of cDNA ends. This virus has a single-stranded RNA genome of 10,540 nucleotides (nt) excluding the poly(A) tail. Two non-overlapping open reading frames (ORFs) in the sense direction were predicted: one long ORF at the 5' end of the genome (6,219 nt) that encodes a polypeptide of 2,072 amino acids (aa), and one short ORF at the 3' end of the genome (3,033 nt) that encodes a polypeptide of 1,010 aa. Phylogenetic analysis indicated that the virus clusters within a large cluster of currently unidentified picorna-like viruses with a high bootstrap value. We named the virus isolate Arma chinensis picorna-like virus 1 (AcPV-1). The prevalence of AcPV-1 infection in samples of Arma chinensis from the wild was at a low level (5.48%, 8 positives in 146 samples). Keywords: Arma chinensis; genomic characterization; phylogenetic analysis; Arma chinensis picorna-like virus 1; prevalence.
期刊介绍：
Acta virologica is an international journal of predominantly molecular and cellular virology. Acta virologica aims to publish papers reporting original results of fundamental and applied research mainly on human, animal and plant viruses at cellular and molecular level. As a matter of tradition, also rickettsiae are included. Areas of interest are virus structure and morphology, molecular biology of virus-cell interactions, molecular genetics of viruses, pathogenesis of viral diseases, viral immunology, vaccines, antiviral drugs and viral diagnostics.
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