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Advances in biochemical engineering/biotechnology最新文献

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Photobiohydrogen Production and Strategies for H2 Yield Improvements in Cyanobacteria. 蓝藻光生物制氢及提高H2产量的策略。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2023-01-01 DOI: 10.1007/10_2023_216
Wanthanee Khetkorn, Wuttinun Raksajit, Cherdsak Maneeruttanarungroj, Peter Lindblad
{"title":"Photobiohydrogen Production and Strategies for H<sub>2</sub> Yield Improvements in Cyanobacteria.","authors":"Wanthanee Khetkorn,&nbsp;Wuttinun Raksajit,&nbsp;Cherdsak Maneeruttanarungroj,&nbsp;Peter Lindblad","doi":"10.1007/10_2023_216","DOIUrl":"https://doi.org/10.1007/10_2023_216","url":null,"abstract":"<p><p>Hydrogen gas (H<sub>2</sub>) is one of the potential future sustainable and clean energy carriers that may substitute the use of fossil resources including fuels since it has a high energy content (heating value of 141.65 MJ/kg) when compared to traditional hydrocarbon fuels [1]. Water is a primary product of combustion being a most significant advantage of H<sub>2</sub> being environmentally friendly with the capacity to reduce global greenhouse gas emissions. H<sub>2</sub> is used in various applications. It generates electricity in fuel cells, including applications in transportation, and can be applied as fuel in rocket engines [2]. Moreover, H<sub>2</sub> is an important gas and raw material in many industrial applications. However, the high cost of the H<sub>2</sub> production processes requiring the use of other energy sources is a significant disadvantage. At present, H<sub>2</sub> can be prepared in many conventional ways, such as steam reforming, electrolysis, and biohydrogen production processes. Steam reforming uses high-temperature steam to produce hydrogen gas from fossil resources including natural gas. Electrolysis is an electrolytic process to decompose water molecules into O<sub>2</sub> and H<sub>2</sub>. However, both these two methods are energy-intensive and producing hydrogen from natural gas, which is mostly methane (CH<sub>4</sub>) and in steam reforming generates CO<sub>2</sub> and pollutants as by-products. On the other hand, biological hydrogen production is more environmentally sustainable and less energy intensive than thermochemical and electrochemical processes [3], but most concepts are not yet developed to production scale.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":"183 ","pages":"253-279"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10118386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Solid-Phase Cell-Free Protein Synthesis and Its Applications in Biotechnology. 固相无细胞蛋白质合成及其在生物技术中的应用。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2023-01-01 DOI: 10.1007/10_2023_226
Mercedes Sánchez-Costa, Fernando López-Gallego
{"title":"Solid-Phase Cell-Free Protein Synthesis and Its Applications in Biotechnology.","authors":"Mercedes Sánchez-Costa,&nbsp;Fernando López-Gallego","doi":"10.1007/10_2023_226","DOIUrl":"10.1007/10_2023_226","url":null,"abstract":"<p><p>Cell-free systems for the in vitro production of proteins have revolutionized the synthetic biology field. In the last decade, this technology is gaining momentum in molecular biology, biotechnology, biomedicine and even education. Materials science has burst into the field of in vitro protein synthesis to empower the value of existing tools and expand its applications. In this sense, the combination of solid materials (normally functionalized with different biomacromolecules) together with cell-free components has made this technology more versatile and robust. In this chapter, we discuss the combination of solid materials with DNA and transcription-translation machinery to synthesize proteins within compartments, to immobilize and purify in situ the nascent protein, to transcribe and transduce DNAs immobilized on solid surfaces, and the combination of all or some of these strategies.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"21-46"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9618154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cell-Free Synthesis and Electrophysiological Analysis of Multipass Voltage-Gated Ion Channels Tethered in Microsomal Membranes. 束缚在微粒体膜中的多通道电压门控离子通道的无细胞合成和电生理分析。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2023-01-01 DOI: 10.1007/10_2023_228
Yogesh Pandey, Srujan Kumar Dondapati, Doreen Wüstenhagen, Stefan Kubick
{"title":"Cell-Free Synthesis and Electrophysiological Analysis of Multipass Voltage-Gated Ion Channels Tethered in Microsomal Membranes.","authors":"Yogesh Pandey,&nbsp;Srujan Kumar Dondapati,&nbsp;Doreen Wüstenhagen,&nbsp;Stefan Kubick","doi":"10.1007/10_2023_228","DOIUrl":"10.1007/10_2023_228","url":null,"abstract":"<p><p>Cell-free protein synthesis (CFPS) has emerged as a powerful tool for the rapid synthesis and analysis of various structurally and functionally distinct proteins. These include 'difficult-to-express' membrane proteins such as large multipass ion channel receptors. Owing to their membrane localization, eukaryotic CFPS supplemented with endoplasmic reticulum (ER)-derived microsomal vesicles has proven to be an efficient system for the synthesis of functional membrane proteins. Here we demonstrate the applicability of the eukaryotic cell-free systems based on lysates from the mammalian Chinese Hamster Ovary (CHO) and insect Spodoptera frugiperda (Sf21) cells. We demonstrate the efficiency of the systems in the de novo cell-free synthesis of the human cardiac ion channels: ether-a-go-go potassium channel (hERG) K<sub>V</sub>11.1 and the voltage-gated sodium channel hNa<sub>V</sub>1.5.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"103-120"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10484377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid and Finely-Tuned Expression for Deployable Sensing Applications. 可部署传感应用的快速微调表达式。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2023-01-01 DOI: 10.1007/10_2023_223
Alexandra T Patterson, Mark P Styczynski
{"title":"Rapid and Finely-Tuned Expression for Deployable Sensing Applications.","authors":"Alexandra T Patterson,&nbsp;Mark P Styczynski","doi":"10.1007/10_2023_223","DOIUrl":"10.1007/10_2023_223","url":null,"abstract":"<p><p>Organisms from across the tree of life have evolved highly efficient mechanisms for sensing molecules of interest using biomolecular machinery that can in turn be quite valuable for the development of biosensors. However, purification of such machinery for use in in vitro biosensors is costly, while the use of whole cells as in vivo biosensors often leads to long sensor response times and unacceptable sensitivity to the chemical makeup of the sample. Cell-free expression systems overcome these weaknesses by removing the requirements associated with maintaining living sensor cells, allowing for increased function in toxic environments and rapid sensor readout at a production cost that is often more reasonable than purification. Here, we focus on the challenge of implementing cell-free protein expression systems that meet the stringent criteria required for them to serve as the basis for field-deployable biosensors. Fine-tuning expression to meet these requirements can be achieved through careful selection of the sensing and output elements, as well as through optimization of reaction conditions via tuning of DNA/RNA concentrations, lysate preparation methods, and buffer conditions. Through careful sensor engineering, cell-free systems can continue to be successfully used for the production of tightly regulated, rapidly expressing genetic circuits for biosensors.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"141-161"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9632310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Barriers to the Use of Medicinal Mushrooms for Production of Metabolites : The Bjarmin Rushton Story. 使用药用蘑菇生产代谢产物的障碍：Bjarmin-Rushton的故事。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2023-01-01 DOI: 10.1007/10_2021_188
Bjørn Kristiansen, M Berovic
{"title":"Barriers to the Use of Medicinal Mushrooms for Production of Metabolites : The Bjarmin Rushton Story.","authors":"Bjørn Kristiansen,&nbsp;M Berovic","doi":"10.1007/10_2021_188","DOIUrl":"10.1007/10_2021_188","url":null,"abstract":"<p><p>Traditionalists are reluctant to leave the technology they and their forefathers knew. To them the new technology based on the stirred tank bioreactor is too removed from the soil the mushroom comes from. On the other hand, there are examples of applications of a bioreactor which support the change from the old to the new technology. In this chapter Bjarmin Rushton, the creator of the well-known medicinal mushroom company Wellness, gives his view of the much talked about difficulties with cultivation in stirred tanks. These problems, are they real or figment of our imagination? Those who read will find out.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":"184 ","pages":"107-123"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10641342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SiLA 2: The Next Generation Lab Automation Standard. SiLA 2：下一代实验室自动化标准。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2022-06-01 DOI: 10.1007/10_2022_204
Daniel Juchli
{"title":"SiLA 2: The Next Generation Lab Automation Standard.","authors":"Daniel Juchli","doi":"10.1007/10_2022_204","DOIUrl":"https://doi.org/10.1007/10_2022_204","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45033675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Overview on Glycosylated Lipids Produced by Bacteria and Fungi: Rhamno-, Sophoro-, Mannosylerythritol and Cellobiose Lipids. 细菌和真菌产生的糖基化脂类综述:鼠李糖、苦参糖、甘露糖赤藓糖醇和纤维素糖脂类。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2022-05-09 DOI: 10.1007/10_2021_200
S. Zibek, G. Soberón-Chávez
{"title":"Overview on Glycosylated Lipids Produced by Bacteria and Fungi: Rhamno-, Sophoro-, Mannosylerythritol and Cellobiose Lipids.","authors":"S. Zibek, G. Soberón-Chávez","doi":"10.1007/10_2021_200","DOIUrl":"https://doi.org/10.1007/10_2021_200","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41883831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Comparison of Laboratory Standards. 实验室标准比较。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2022-04-24 DOI: 10.1007/10_2022_205
Matthias Freundel
{"title":"Comparison of Laboratory Standards.","authors":"Matthias Freundel","doi":"10.1007/10_2022_205","DOIUrl":"https://doi.org/10.1007/10_2022_205","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48978988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Systems Biology on Acetogenic Bacteria for Utilizing C1 Feedstocks. 产丙酮菌利用C1原料的系统生物学研究。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2022-04-10 DOI: 10.1007/10_2021_199
Yoseb Song, Jiyun Bae, Jongoh Shin, Sangrak Jin, Seulgi Kang, H. Lee, Suhyung Cho, Byung-Kwan Cho
{"title":"Systems Biology on Acetogenic Bacteria for Utilizing C1 Feedstocks.","authors":"Yoseb Song, Jiyun Bae, Jongoh Shin, Sangrak Jin, Seulgi Kang, H. Lee, Suhyung Cho, Byung-Kwan Cho","doi":"10.1007/10_2021_199","DOIUrl":"https://doi.org/10.1007/10_2021_199","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47331238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Engineering the Reductive Glycine Pathway: A Promising Synthetic Metabolism Approach for C1-Assimilation. 工程还原甘氨酸途径:c1同化的一种有前途的合成代谢途径。
4区 工程技术
Advances in biochemical engineering/biotechnology Pub Date : 2022-04-02 DOI: 10.1007/10_2021_181
Nico J. Claassens, Ari Satanowski, Viswanada R Bysani, Beau Dronsella, E. Orsi, Vittorio Rainaldi, S. Yilmaz, Sebastian Wenk, Steffen N. Lindner
{"title":"Engineering the Reductive Glycine Pathway: A Promising Synthetic Metabolism Approach for C1-Assimilation.","authors":"Nico J. Claassens, Ari Satanowski, Viswanada R Bysani, Beau Dronsella, E. Orsi, Vittorio Rainaldi, S. Yilmaz, Sebastian Wenk, Steffen N. Lindner","doi":"10.1007/10_2021_181","DOIUrl":"https://doi.org/10.1007/10_2021_181","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42766243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
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