Advances in biochemical engineering/biotechnology最新文献

{"title":"Technical Scale in Biomolecular Crystallization.","authors":"Wenqing Tian, Wenqian Chen, Huaiyu Yang","doi":"10.1007/10_2025_301","DOIUrl":"10.1007/10_2025_301","url":null,"abstract":"<p><p>In recent years, the scalability of biomolecular crystallization processes has garnered significant attention due to its advantages in enhancing product quality and reducing manufacturing costs compared to the traditional purification methods. Unlike the crystallization process of small molecules, biomolecular crystallization lacks a comprehensive theory or guidelines, often relying on modified crystallization theories and empirical approaches. It is challenging to guide protein molecules assembling into ordered crystal lattice due to the huge molecular size and complexity of the structure, and protein crystals are more delicate and fragile than conventional small organic molecular crystals. Furthermore, proteins often exhibit limited stability within specific environmental conditions, such as varying pH and temperature ranges. Consequently, many standard methods employed for small-molecule crystallization are not applicable to biomolecular crystallization. Most of the bimolecular crystallization research are focused on tiny scale, while more research on biomolecular crystallization at various larger technical scales has emerged over time. The scaling up mechenism from the tiny scale to the large scale is critical to widely apply the biomolecular crystallization. This chapter begins by summarizing the different methods used in biomolecular crystallization. Then, biomolecular crystallization is reviewed from nL to μL scale (e.g., hanging drop or sitting drop) to mL scale, such as in small batch or microfluidic crystallizer till hundred mL or L scale, such as standard stirred crystallizer. Finally, it explores different crystallization scales with corresponding process monitoring and analytical methods for the crystallization kinetics.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"123-147"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Numerical and Analytical Methodologies.","authors":"Tomaž Urbič, Igor Plazl","doi":"10.1007/10_2026_330","DOIUrl":"10.1007/10_2026_330","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"419"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148142304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: The Human Gut Microbiota: A Dynamic Biologic Factory.","authors":"Alireza Minagar, Rabih Jabbour","doi":"10.1007/10_2024_253","DOIUrl":"10.1007/10_2024_253","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"243"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140891015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Human Gut Microbiota: A Dynamic Biologic Factory.","authors":"Alireza Minagar, Rabih Jabbour","doi":"10.1007/10_2023_243","DOIUrl":"10.1007/10_2023_243","url":null,"abstract":"<p><p>The human body constitutes a living environment for trillions of microorganisms, which establish the microbiome and, the largest population among them, reside within the gastrointestinal tract, establishing the gut microbiota. The term \"gut microbiota\" refers to a set of many microorganisms [mainly bacteria], which live symbiotically within the human host. The term \"microbiome\" means the collective genomic content of these microorganisms. The number of bacterial cells within the gut microbiota exceeds the host's cells; collectively and their genes quantitatively surpass the host's genes. Immense scientific research into the nature and function of the gut microbiota is unraveling its roles in certain human health activities such as metabolic, physiology, and immune activities and also in pathologic states and diseases. Interestingly, the microbiota, a dynamic ecosystem, inhabits a particular environment such as the human mouth or gut. Human microbiota can evolve and even adapt to the host's unique features such as eating habits, genetic makeup, underlying diseases, and even personalized habits. In the past decade, biologists and bioinformaticians have concentrated their research effort on the potential roles of the gut microbiome in the development of human diseases, particularly immune-mediated diseases and colorectal cancer, and have initiated the assessment of the impact of the gut microbiome on the host genome. In the present chapter, we focus on the biological features of gut microbiota, its physiology as a biological factory, and its impacts on the host's health and disease status.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"91-106"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139711174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Mechanism of Action of Illicium simonsii Maxim. in the Treatment of Neurodegenerative Diseases Based on Mass Spectrometry Coupling Technology and Network Pharmacology","authors":"Xiaotong Xu, Xueping Liang, Ya Liu, Yijia Liu, Zijian Huang, Liying Wang, Wenzhong Hu","doi":"10.2991/978-94-6463-829-5_24","DOIUrl":"https://doi.org/10.2991/978-94-6463-829-5_24","url":null,"abstract":"","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":"1 1","pages":"234-246"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147882854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introduction to the Use of Microbial Communities.","authors":"Elias Hakalehto","doi":"10.1007/10_2024_265","DOIUrl":"10.1007/10_2024_265","url":null,"abstract":"<p><p>Microbes are the third major group of biospheric organisms after plants and animals. They are responsible for many natural circulations, including the rotation of elements. They return organic carbon for plants' use and dissolve minerals into organic cycles. Microbes contribute to the global gas and water balances. In animal digestion, they partake in the degradation and assimilation of nutrients. Typically, they act as communities where some strains are the most active at a given time point in the prevailing conditions. But they also live in a continuous state of succession, which precludes the maintenance of changeable balances. Whether functioning in soil, in our alimentary tract, or elsewhere, the micro-organisms decisively contribute to the restoration of various balances. As the microbiological scale differs significantly from our comprehension, we must nurture our understanding of the microbiome wherever it occurs. For example, one spoonful of yoghurt contains approximately as many bacterial cells as there are humans residing on Earth. Therefore, flexibility and interaction are the most advisable modes of operation in microbial biochemistry and biotechnological applications. As microbes tend to form communities, this modus operandi is worth instigating in our process industries and production technologies. The use of microbial mixed cultures most appropriately corresponds to the natural systems [1]. As biocatalysts in human endeavours of biorefining and bioengineering, they have become the most utilizable and producible kind of microbial components. Cooperation with microbes is a prerequisite for the continuous development of sustainable industries and food and health production. The microbial communities can be used to prevent and clean up pollution. In the process design, the microbiological dynamic balances make the highest productivity, repeatability, controllability, and withstanding of entropy. Although their effects have been familiar to our societies, e.g. in the fermentation of foods, their total capacity remains to be put into service. Hopefully, this book could help turn the next page in the development.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploitation of Biodiversity in Bioeconomy: Examples, Opportunities, and Challenges.","authors":"Alexander Grünberger, Emily Schepp, Selina Lang, Kevin Edward Schulz, Daniel Baron Diaz, Arabi Sivanesapillai, Andreas Diepold, Camilla Stolle, Kersten Rabe, Roland Ulber, Dirk Holtmann","doi":"10.1007/10_2025_288","DOIUrl":"10.1007/10_2025_288","url":null,"abstract":"<p><p>While bioprocesses using Escherichia coli, Corynebacterium glutamicum, various species of Bacillus, lactic acid bacteria, Clostridia, the yeasts Saccharomyces cerevisiae and Pichia pastoris, fungi such as Aspergillus niger, and Chinese hamster ovary cells are well established, the high level of microbial diversity has not yet been exploited industrially. However, the use of alternative organisms has the potential to significantly expand the process window of bioprocesses. These extensions include the use of alternative substrates (e.g., CO₂, syngas, or methane), unusual process conditions (e.g., temperatures, media conditions), or use of microbial diversity itself (e.g., biofilm growth, alternative electron transport systems, product secretion, surface presentation of enzymes). Bringing these hidden champions into application will enable more efficient processes with environmental and ecological benefits. Some of the most promising alternative microorganisms are described and discussed in detail in this book. The aim of this introductory chapter is to provide an overview of additional organisms and cultivation strategies. Finally, it addresses future challenges to bring the organism into technical applications.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"1-25"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vibrio natriegens: Application of a Fast-Growing Halophilic Bacterium.","authors":"Maurice Hädrich, Clarissa Schulze, Josef Hoff, Bastian Blombach","doi":"10.1007/10_2024_271","DOIUrl":"10.1007/10_2024_271","url":null,"abstract":"<p><p>The fast growth accompanied with high substrate consumption rates and a versatile metabolism paved the way to exploit Vibrio natriegens as unconventional host for biotechnological applications. Meanwhile, a wealth of knowledge on the physiology, the metabolism, and the regulation in this halophilic marine bacterium has been gathered. Sophisticated genetic engineering tools and metabolic models are available and have been applied to engineer production strains and first chassis variants of V. natriegens. In this review, we update the current knowledge on the physiology and the progress in the development of synthetic biology tools and provide an overview of recent advances in metabolic engineering of this promising host. We further discuss future challenges to enhance the application range of V. natriegens.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"85-116"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium Removal from Wastewater by Microbial Transformation and Volatilization.","authors":"Tochukwu Ekwonna, Olusegun Akindeju, Brianna Amos, Zhi-Qing Lin","doi":"10.1007/10_2023_242","DOIUrl":"10.1007/10_2023_242","url":null,"abstract":"<p><p>Selenium (Se) is a naturally occurring trace element that is nutritionally essential for humans and animals, but becomes toxic at high concentrations. This laboratory study explored the role of microbes in Se removal from contaminated wastewater via biological transformation and volatilization processes. Microbes could immobilize water-soluble selenate (SeO₄^2-) and selenite (SeO₃^2-) to water-insoluble elemental Se (Se⁰) and transform Se into volatile Se compounds found in the atmosphere. Results of this laboratory study showed that Bacillus cereus, a bacterial strain isolated from wheat straw and biosolid-WTR-sand substrates showed a significant biotransformation ability of reducing selenate and selenite to elemental Se and forming volatile Se organic compounds in wastewater. Overall, microbial Se chemical reduction, methylation, and volatilization are important processes in bioremediation of Se-contaminated wastewater.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"125-136"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139711172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable Approaches in Viticulture: From Wastes and Side Streams to High-Value Products.","authors":"Niël van Wyk, Claudia Borgmeier, Alice Kleber, Esther M Gabor","doi":"10.1007/10_2025_281","DOIUrl":"10.1007/10_2025_281","url":null,"abstract":"<p><p>In the context of climate change, resource scarcity, and a growing global population, sustainable processes and the efficient use of raw materials are imperative. Developing best practices to guide the transition toward a circular economy with minimized waste is essential. Agricultural processes offer inspiration, as they traditionally emphasize the utilization of all parts of cultivated plants. Today, however, there is a pressing need to go beyond basic utilization and focus on the upcycling and valorization of agricultural side streams into high-value products. This chapter examines the wine and grape industry, which generates substantial volumes of side streams, including grape pomace, seeds, stems, and wine lees. In recent years, these material streams have attracted attention for their potential as raw materials rich in bioactive compounds - such as diverse polyphenols, dietary fibers, and organic acids - that can be applied across various sectors, including food, cosmetics, pharmaceuticals, and bioenergy. Key methodologies such as extraction, fermentation, and bioconversion are discussed as pathways to recover and enhance these compounds. Case studies of successful valorization initiatives are presented, demonstrating practical applications of side streams for high-value product creation. An example is the BMBF-funded project \"Sustainable Beverages\" focusing on the development of fermented drinks made from vine leaves that can be offered as a non-alcoholic alternative to wine. By providing an overview of the current research and technologies in wine waste valorization, this chapter serves as a valuable resource for academics, industry professionals, and policymakers seeking to advance sustainable practices within the wine sector. Ultimately, the upcycling of wine industry residues not only reduces environmental impact but also creates new revenue opportunities, driving innovation and sustainability in the industry.</p>","PeriodicalId":7198,"journal":{"name":"Advances in biochemical engineering/biotechnology","volume":" ","pages":"151-184"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}