Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology最新文献_第7页

An investigation of a collection of yellow-pigmented Pseudomonas. 黄色假单胞菌群的调查。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-06-01 DOI: 10.1111/j.1699-0463.1986.tb03034.x

P Søgaard, B G Hansen, W Frederiksen

{"title":"An investigation of a collection of yellow-pigmented Pseudomonas.","authors":"P Søgaard, B G Hansen, W Frederiksen","doi":"10.1111/j.1699-0463.1986.tb03034.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03034.x","url":null,"abstract":"Thirty-six strains of yellow-pigmented Pseudomonas from clinical as well as non-clinical material and 11 reference strains of Pseudomonas were investigated by means of conventional bacteriological methods (a total of 53 different tests). Eighteen of the 36 yellow-pigmented strains could be classified as P. paucimobilis. Apart from the presence of lipid inclusions on beta-hydroxybutyrate, hydrolysis of DNA, and Tween 80 our results showed a high degree of accordance with other investigations. Eight strains showed characteristics compatible with inclusion in the CDC VE group; one orange-yellow strain showed the characteristics of P. vesicularis, and one was a pyoverdin negative, yellow P. putida. Eight strains remained unidentified. Strains of P. paucimobilis were most often resistant to antibiotics used for P. aeruginosa infections (viz. piperacillin, cefsulodin, ceftazidime) while the strains of the CDC VE group were often susceptible. Most strains were susceptible to the aminoglycosides. The difficulties in distinguishing yellow-pigmented strains of Pseudomonas from Flavobacterium spp. or Xanthomonas spp. are discussed.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 3","pages":"145-52"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03034.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14858999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Susceptibility testing with disc diffusion for new cephalosporins: pre-diffusion revisited. 新型头孢菌素的盘扩散药敏试验:扩散前再访。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-06-01 DOI: 10.1111/j.1699-0463.1986.tb03036.x

N Frimodt-Møller, E Hvass, T Højbjerg, S Møller, I Mortensen, V F Thomsen

{"title":"Susceptibility testing with disc diffusion for new cephalosporins: pre-diffusion revisited.","authors":"N Frimodt-Møller, E Hvass, T Højbjerg, S Møller, I Mortensen, V F Thomsen","doi":"10.1111/j.1699-0463.1986.tb03036.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03036.x","url":null,"abstract":"The purpose of the present study was to investigate the effect of 20-hour pre-diffusion, i.e. placing the antibiotic-containing discs on the agar for 20 hours prior to inoculation, as compared to direct diffusion, i.e. placing the discs on the agar immediately after inoculation, for the newer 3. generation cephalosporins as represented by ceftazidime and ceftriaxone. Regression lines (zone sizes vs. inhibitory concentrations, as measured by plate-dilution) were constructed for three groups of bacteria chosen because of their differences in growth characteristics on agar: E. coli (n = 50), Enterobacter sp. (n = 35), and streptococci (n = 51). The results for both cephalosporins were: 20-hour pre-diffusion produced larger zones than direct diffusion, regression studies for 20-hour pre-diffusion as compared to direct diffusion resulted in greater variation in zone sizes, numerically lower slopes, lower residual variances and higher correlation coefficients, and regression lines were significantly different for the 3 groups of bacteria with direct diffusion but not so with 20-hour pre-diffusion. Considering the interpretation of zone sizes with disc diffusion for the cephalosporins tested, 20-hour pre-diffusion was superior to direct diffusion.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 3","pages":"159-66"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03036.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14222078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effects of carbon dioxide upon the in vitro activity of erythromycin. 二氧化碳对红霉素体外活性的影响。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-06-01 DOI: 10.1111/j.1699-0463.1986.tb03038.x

W L Dibb, A Digranes, K L Bottolfsen

{"title":"Effects of carbon dioxide upon the in vitro activity of erythromycin.","authors":"W L Dibb, A Digranes, K L Bottolfsen","doi":"10.1111/j.1699-0463.1986.tb03038.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03038.x","url":null,"abstract":"The in vitro activity of erythromycin against clinical isolates of Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Streptococcus pyogenes and Haemophilus influenzae was examined by agar dilution and agar diffusion methods. The plates were incubated in air alone or in 8% CO2 and air. The minimal inhibitory concentrations (MICs) measured in air alone were lower for most of the isolates, compared to those found in 8% CO2. The greatest differences in MIC values were found for H. influenzae; the MIC 50% was 0.5 mg/l in air and 4 mg/l in 8% CO2. Sensitivity testing by the agar diffusion method (ICS) showed considerable differences between results obtained in air and in 8% CO2; the inhibition zones were generally smaller in CO2. The most marked reduction in zone sizes after incubation in 8% CO2 was seen with the H. influenzae isolates; 15 out of 43 isolates moved from the \"sensitive\" to \"moderately sensitive\" group. Sensitivity determination of aerobic bacteria for erythromycin should be performed in air alone in the routine laboratory.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 3","pages":"173-6"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03038.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14221967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Re-investigations of selected bovine and ovine strains previously classified as Pasteurella haemolytica and description of some new taxa within the Pasteurella haemolytica-complex. 先前分类为溶血巴斯德氏菌的牛和羊菌株的重新调查和溶血巴斯德氏菌复合体中一些新分类群的描述。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-06-01 DOI: 10.1111/j.1699-0463.1986.tb03040.x

M Bisgaard, R Mutters

引用次数: 40

Production of toxin in strains previously classified as Pasteurella multocida. 以前被归类为多杀性巴氏杆菌的菌株产生毒素。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-06-01 DOI: 10.1111/j.1699-0463.1986.tb03042.x

J P Nielsen, M Bisgaard, K B Pedersen

引用次数: 23

Influence of polysaccaride capsule and ionic strength on buoyant density of group B streptococci. 多糖胶囊及离子强度对B群链球菌浮力密度的影响。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-06-01 DOI: 10.1111/j.1699-0463.1986.tb03033.x

S Håkansson, S Holm

引用次数: 11

Characterization of some previously unclassified "Pasteurella" spp. obtained from the oral cavity of dogs and cats and description of a new species tentatively classified with the family Pasteurellaceae Pohl 1981 and provisionally called taxon 16. 从狗和猫的口腔中获得的一些以前未分类的“巴斯德氏菌”的特征和一个暂定为巴斯德氏菌科Pohl 1981的新物种的描述，暂时称为16分类群。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-06-01 DOI: 10.1111/j.1699-0463.1986.tb03039.x

M Bisgaard, R Mutters

{"title":"Characterization of some previously unclassified \"Pasteurella\" spp. obtained from the oral cavity of dogs and cats and description of a new species tentatively classified with the family Pasteurellaceae Pohl 1981 and provisionally called taxon 16.","authors":"M Bisgaard, R Mutters","doi":"10.1111/j.1699-0463.1986.tb03039.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03039.x","url":null,"abstract":"The taxonomic relationship of 23 unclassified canine and feline strains of Pasteurellaceae and five strains received as Pasteurella spp. or Haemophilus influenzae-murium was investigated by phenotypic and genetic characterization. Eleven strains were classified with four recently described species of genus Pasteurella sensu stricto. Fourteen canine and feline strains formed a homogeneous group, tentatively designated taxon 16. Both phenotypic characters and mol% G + C in DNA and genome size indicate classification of taxon 16 with genus Pasteurella. DNA/DNA hybridizations, however, failed to locate taxon 16 on genus level with accepted or proposed species of the family Pasteurellaceae Pohl 1981. Two additional species obtained from rats and mice remained unclassified, in addition to a human isolate obtained from a dog-bite. The necessity of detailed phenotypic characterization within the family Pasteurellaceae Pohl 1981 needs to be stressed.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 3","pages":"177-84"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03039.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14612607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Two separate non-immune interactions between staphylococcal protein A and immunoglobulins are mediated by structures on gamma chains. 葡萄球菌蛋白A和免疫球蛋白之间的两个独立的非免疫相互作用是由γ链上的结构介导的。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-04-01 DOI: 10.1111/j.1699-0463.1986.tb03022.x

M Erntell, E B Myhre, G Kronvall

{"title":"Two separate non-immune interactions between staphylococcal protein A and immunoglobulins are mediated by structures on gamma chains.","authors":"M Erntell, E B Myhre, G Kronvall","doi":"10.1111/j.1699-0463.1986.tb03022.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03022.x","url":null,"abstract":"The present investigation was undertaken to determine whether the light or the heavy immunoglobulin chain is involved in the alternative, non-immune F(ab')2-mediated binding to staphylococcal protein A. Purified human polyclonal IgG was mildly reduced with dithiothreitol and alkylated with iodoacetamide. Intact IgG, purified light and heavy chains of polyclonal immunoglobulin G were tested in an inhibition assay for alternative non-immune F(ab')2-mediated binding to the protein A-carrying S. aureus, strain Cowan I. The IgG Fc-mediated binding to protein A was studied in parallel inhibition experiments. Heavy chains inhibited both the alternative F(ab')2- and the classical Fc-mediated binding to protein A. Isolated light chains were non-reactive. Intact IgG molecules were more potent inhibitors than isolated heavy chains tested in equimolar concentrations. Our results indicate that the alternative non-immune interaction between staphylococcal protein A and human immunoglobulins is mediated by structures expressed on the heavy immunoglobulin G chain. Thus, there are two separate protein A binding sites on gamma chains.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 2","pages":"69-73"},"PeriodicalIF":0.0,"publicationDate":"1986-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03022.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14220687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Branhamella catarrhalis: significance in pulmonary infections and bacteriological features. 卡他罕氏菌在肺部感染中的意义及细菌学特征。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-04-01 DOI: 10.1111/j.1699-0463.1986.tb03025.x

J J Christensen, O Gadeberg, B Bruun

{"title":"Branhamella catarrhalis: significance in pulmonary infections and bacteriological features.","authors":"J J Christensen, O Gadeberg, B Bruun","doi":"10.1111/j.1699-0463.1986.tb03025.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03025.x","url":null,"abstract":"A three-month survey revealed 29 patients at our hospital with symptoms of acute pulmonary infection, from whom Branhamella catarrhalis was isolated from lower respiratory tract specimens, in 18 cases in pure culture. Approximately 2% of all respiratory tract specimens examined during the period yielded growth of B. catarrhalis. All except one patient suffered from chronic pulmonary disease, notably chronic bronchitis. A phenotypic comparison was made between 55 strains of B. catarrhalis, of which 50 were recent isolates from lower respiratory tract specimens, and 23 Neisseria strains representing Neisseria meningitidis, Neisseria gonorrhoeae, Neisseria cinerea, Neisseria flavescens, Neisseria mucosa, Neisseria pharyngis, and Neisseria lactamica. The morphology of B. catarrhalis colonies is very characteristic, and when the diagnosis is suspected, testing for the ability to hydrolyze tributyrin may confirm it within hours. Ability to produce deoxyribonuclease is another property which differentiates B. catarrhalis from the Neisseria species. Otherwise, the combination of nitrate reduction and failure to produce acid from glucose, maltose, and sucrose establishes the diagnosis.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 2","pages":"89-95"},"PeriodicalIF":0.0,"publicationDate":"1986-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03025.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14846745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Comparative thermoresistance of two biological indicators for monitoring steam autoclaves. 3. Comparison performed at 121 degrees C in a hospital prevacuum steam sterilizer. 监测蒸汽高压灭菌器的两种生物指示剂的耐热性比较。3.在121℃的医院预真空蒸汽灭菌器中进行比较。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-04-01 DOI: 10.1111/j.1699-0463.1986.tb03028.x

N Skaug, T Kalager

{"title":"Comparative thermoresistance of two biological indicators for monitoring steam autoclaves. 3. Comparison performed at 121 degrees C in a hospital prevacuum steam sterilizer.","authors":"N Skaug, T Kalager","doi":"10.1111/j.1699-0463.1986.tb03028.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03028.x","url":null,"abstract":"According to Pharmacopoea Nordica, steam autoclaves should be regularly monitored by a specific Swedish preparation of Bacillus stearothermophilus spores. If another biological indicator (BI) is used for such a control, it should first be calibrated against the Swedish BI (SBI) and the two BIs should be equally thermoresistant. Attest No. 1262 BI (ABI) has previously been shown to be more thermoresistant than the SBI at 134 degrees C, saturated steam. The purpose of the present study was to compare the thermoresistance of the SBI and the ABI at 121 degrees C, saturated steam and prevacuum. Seven hundred and twenty units of each BI were heat-exposed in an Emmer 760 litre prevacuum, pressure-pulsing steam autoclave. After prevacuum with steam injection (manual or automatic preconditioning), the following incremental heat exposure times were used in triplicate (20 simultaneously tested units of each BI in each cycle) according to a randomized scheme: 5, 6 1/2, 8, 9 1/2, 11, 12 1/2, 14 and 15 min. The intra-chamber pressure and temperature were continuously monitored throughout the test and equilibration cycles. The heat-exposed BI units were cultivated and read as recommended by the manufacturers. SBI and ABI showed a survival-time of 8 min and 11 min respectively, and a kill-time between 14 min and 15 min for both BIs. Thus, the ABI had the narrower survival-kill window. Probit analysis testing of the results showed that the difference in thermoresistance, at 121 degrees C, saturated steam and prevacuum between Attest No. 1262 BI and the Swedish BI mentioned in Pharmacopea Nordica was not statistically significant.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 2","pages":"107-12"},"PeriodicalIF":0.0,"publicationDate":"1986-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03028.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14847800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2