Acta Pharmaceutica最新文献_第2页

H3 relaxin mediated neuroprotection in Alzheimer's disease pathology induced by streptozotocin in mouse models: Impact on memory improvement, autophagy and PI3K/Akt-mTOR signalling pathway. 链脲佐菌素诱导小鼠阿尔茨海默病病理中H3松弛素介导的神经保护：对记忆改善、自噬和PI3K/Akt-mTOR信号通路的影响

IF 1.8 4区医学

Acta Pharmaceutica Pub Date : 2026-03-25 Print Date: 2026-03-01 DOI: 10.2478/acph-2026-0009

Huiyu Zhao, Yuhong Sun, Shaik Althaf Hussain, Hua Gao

{"title":"H3 relaxin mediated neuroprotection in Alzheimer's disease pathology induced by streptozotocin in mouse models: Impact on memory improvement, autophagy and PI3K/Akt-mTOR signalling pathway.","authors":"Huiyu Zhao, Yuhong Sun, Shaik Althaf Hussain, Hua Gao","doi":"10.2478/acph-2026-0009","DOIUrl":"10.2478/acph-2026-0009","url":null,"abstract":"Alzheimer's disease (AD) is characterised by β-amyloid (Aβ) plaque accumulation and tau hyperphosphorylation. H3 relaxin, a neuro-peptide, is known to exert neuroprotective effects. In this study, we investigated how H3 relaxin confers neuroprotection in a streptozotocin (STZ)-induced mouse model and modulates PI3K/Akt-mTOR signalling. Mice were divided into four groups (n = 6 per group): control (saline), STZ, STZ + H3 relaxin, and STZ + donepezil. Following STZ induction, H3 relaxin (1 µg per day) was administered intracerebro ventricularly (ICV) for 14 consecutive days, whereas donepezil (2.5 mg kg-1 per day) was administered orally for the same duration. Cognitive performance was assessed using the Morris water maze (MWM) test. Aβ deposition in the cortex was evaluated through immunohistochemistry. Western blotting was conducted for tau phospho rylation, PI3K/Akt/mTOR signalling, and autophagy markers in the hippocampus. Oxidative stress and inflammation markers were measured using ELISA. H3 relaxin markedly improved memory by decreasing escape latency and duration while spending more time in the target quadrant in the MWM test. Additionally, H3 relaxin reduced Aβ plaque burden and tau phosphorylation (Ser396/404) while enhancing PI3K/Akt-mTOR signalling. Oxidative stress was attenuated, as evidenced by increased GSH and HO-1 levels and reduced MDA and H2O2 concentrations. Moreover, markers of inflammation, NF-κB and TNF-α were suppressed. Overall, H3 relaxin ameliorated cognitive deficits in STZ-induced AD mice through modulation of impaired PI3K/Akt-mTOR signalling, reduction of Aβ and tau pathology, and promotion of autophagy.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":"76 1","pages":"1-18"},"PeriodicalIF":1.8,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sedation management in mechanically ventilated intensive care unit patients: Meta-analysis review. 机械通气重症监护病房患者的镇静管理：meta分析综述。

IF 1.4 4区医学

Acta Pharmaceutica Pub Date : 2026-03-25 Print Date: 2026-03-01 DOI: 10.2478/acph-2026-0003

Fawei Yuan, Dong Liu

{"title":"Sedation management in mechanically ventilated intensive care unit patients: Meta-analysis review.","authors":"Fawei Yuan, Dong Liu","doi":"10.2478/acph-2026-0003","DOIUrl":"10.2478/acph-2026-0003","url":null,"abstract":"Sedation is crucial for managing mechanically ventilated intensive care unit (ICU) patients, but agents differ in their effects. Propofol, benzodiazepines and α 2-agonists are commonly used, yet their comparative impact remains unclear. This review searched OVID MEDLINE and Embase from January 2006 to June 2025 for randomised controlled trials in adult ICU patients. The primary outcome was duration of mechanical ventilation; secondary outcomes were ICU length of stay, delirium and mortality. Twenty-six trials (N = 4,993) were included. Dexmedetomidine significantly shortened mechanical ventilation (mean difference [MD] -0.60 days; 95 % CI -0.89 to -0.31), with larger effects versus midazolam (MD -1.25 days) and mixed comparators (MD -1.23 days), but not versus propofol (MD -0.34 days). ICU stay was also reduced (MD -0.94 days; 95 % CI -1.49 to -0.39). Delirium risk decreased (odds ratio [OR] 0.58; 95 % CI 0.38-0.87). No mortality difference was found. Dexmedetomidine is therefore associated with a modest but clinically meaningful reduction in ventilation time, ICU stay and delirium, particularly when compared with benzodiaze-pines, though benefits over propofol are less certain.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":" ","pages":"1-13"},"PeriodicalIF":1.4,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145909989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sexually dimorphic and time-dependent influence of active avoidance learning by vilazodone in C57BL/6J mice. 维拉唑酮对C57BL/6J小鼠主动回避学习的两性二态和时间依赖性影响。

IF 1.8 4区医学

Acta Pharmaceutica Pub Date : 2026-03-25 Print Date: 2026-03-01 DOI: 10.2478/acph-2026-0007

Kang Hu, Anran Zhang, Yuan Shi, Huizhen Yuan, Xiangxi Kong, Jun Ma

{"title":"Sexually dimorphic and time-dependent influence of active avoidance learning by vilazodone in C57BL/6J mice.","authors":"Kang Hu, Anran Zhang, Yuan Shi, Huizhen Yuan, Xiangxi Kong, Jun Ma","doi":"10.2478/acph-2026-0007","DOIUrl":"10.2478/acph-2026-0007","url":null,"abstract":"The effects of vilazodone (VZD) on the acquisition of active avoidance behavior were examined in C57BL/6J mice. Both female and male mice were assigned to three groups (n = 8 per group per sex): the vehicle control group (VEH), the 0.5 mg kg-1 vilazodone lower dose group (VZD0.5) and the 1 mg kg-1 vilazodone higher dose group (VZD1.0). Spontaneous locomotion and anxiety-like behaviour were assessed after drug administration intraperitoneally in an open field test (OFT). Another cohort of mice was trained in a three-day shuttle box active avoidance test (AAT) after drug administrations with the aim of evaluating the effects of VZD on the acquisition of active avoidance behaviour. In the OFT, VZD decreased freezing time in the corner area in both female and male mice, indicating reduced anxiety-like behaviours. In the AAT, the active avoidance rate was significantly improved on day 1 in female mice and day 2 in male mice, suggesting that VZD facilitated active avoidance learning with sexual dimorphism. Furthermore, the increased active avoidance rates were negatively correlated with freezing time during training. Interestingly, these group differences and correlations diminished on day 3, implying that the facilitation was restricted to early training phases. Collectively, VZD facilitates the acquisition of active avoidance behaviour in mice with distinct sexual dimorphism and temporal dynamics.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":"76 1","pages":"1-15"},"PeriodicalIF":1.8,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beneficial effect of omega-3 fatty acids supplementation on leaky gut, inflammation and oxidative stress in propionic acid-induced autism in aged rats. 补充omega-3脂肪酸对丙酸诱导的老年孤独症大鼠漏肠、炎症和氧化应激的有益影响。

IF 1.8 4区医学

Acta Pharmaceutica Pub Date : 2026-03-25 Print Date: 2026-03-01 DOI: 10.2478/acph-2026-0008

Amal Aloud, Hayat Alzahrani, Shaista Arzoo, Raeesa Mohammed, Islam A Alredah, Alaa R Khatab

{"title":"Beneficial effect of omega-3 fatty acids supplementation on leaky gut, inflammation and oxidative stress in propionic acid-induced autism in aged rats.","authors":"Amal Aloud, Hayat Alzahrani, Shaista Arzoo, Raeesa Mohammed, Islam A Alredah, Alaa R Khatab","doi":"10.2478/acph-2026-0008","DOIUrl":"10.2478/acph-2026-0008","url":null,"abstract":"This study examined the effects of omega-3 fatty acids supplementation on gut barrier integrity, systemic inflammation, neurotrans-mission and oxidative stress, in an aged rat model of propionic acid (PPA)-induced neurotoxicity. Twenty-four aged male rats were divided into four groups: control, omega-3, PPA and PPA + omega-3. Serum cytokines, tight-junction proteins (TJP1), dopamine, serotonin, short-chain fatty acids (SCFAs), oxidative stress markers, and histopathology of the brain and small intestine were evaluated. PPA exposure significantly increased tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and reduced TJP1 expression, confirming gut barrier disruption and systemic inflammation. Omega-3 fatty acids supplementation selectively reduced IL-6 but did not reverse PPA-induced TNF-α elevation or oxidative stress. CLDN2 expression increased in PPA + omega-3 rats, suggesting a compensatory but incomplete barrier response. Dopamine, serotonin, and SCFA levels showed upward trends with supplementation but were not statistically significant. Histological analysis demonstrated partial preservation of neuronal and intestinal structure in the PPA + omega-3 group. Overall, omega-3 fatty acids exerted modest anti-inflammatory effects but failed to fully restore oxidative balance or barrier integrity in aged rats, suggesting that omega-3 fatty acids may be more effective as a preventive rather than restorative intervention in ageing-related gut-brain axis disruption.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":"76 1","pages":"1-17"},"PeriodicalIF":1.8,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gastrointestinal risk profile of tigecycline, omadacycline and eravacycline: Evidence from the FDA adverse event reporting system. 替加环素、奥马达环素和依瓦环素的胃肠道风险概况：来自FDA不良事件报告系统的证据

IF 1.4 4区医学

Acta Pharmaceutica Pub Date : 2026-03-25 Print Date: 2026-03-01 DOI: 10.2478/acph-2026-0001

Zhu Wang, Guoping Gan, Haiping Yao

{"title":"Gastrointestinal risk profile of tigecycline, omadacycline and eravacycline: Evidence from the FDA adverse event reporting system.","authors":"Zhu Wang, Guoping Gan, Haiping Yao","doi":"10.2478/acph-2026-0001","DOIUrl":"10.2478/acph-2026-0001","url":null,"abstract":"This study assessed the gastrointestinal (GI) safety profiles of tigecycline, omadacycline, and eravacycline through a retrospective disproportionality analysis of reports submitted to the FDA Adverse Event Reporting System (FAERS) between the second quarter of 2005 and the first quarter of 2024. Among 3,261 adverse event reports associated with these agents, 809 (24.8 %) involved gastrointestinal disorders, with tigecycline accounting for the largest proportion (588 reports), followed by omadacycline (197) and eravacycline (24). Disproportionality analysis revealed that gastrointestinal disorders ranked among the top three system organ classes for all three drugs, with positive signals observed for tigecycline (ROR = 1.63), omadacycline (ROR = 3.04), and eravacycline (ROR = 1.79), the strongest association being with omadacycline. While most GI events were consistent with known safety information, several unexpected signals were identified, including gastrointestinal haemorrhage, melena, small-intestinal obstruction, tongue discolouration, and intestinal perforation for tigecycline, as well as lip swelling and tongue discolouration for omadacycline. The median onset times of GI events were 4, 0, and 2.5 days for tigecycline, omadacycline, and eravacycline, respectively, with nearly half of the events occurring within three days of treatment initiation. These findings reveal distinct GI safety patterns among newer tetracycline-derived antibiotics and underscore the importance of early and route-specific monitoring in clinical practice.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":" ","pages":"1-20"},"PeriodicalIF":1.4,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145779695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predictive analytical workflow for rapid structure elucidation and in silico toxicological qualification of an unidentified impurity in cefixime granules for oral suspension. 口服混悬剂头孢克肟颗粒中未知杂质的快速结构解析和硅毒性鉴定的预测分析工作流程。

IF 1.8 4区医学

Acta Pharmaceutica Pub Date : 2026-03-25 Print Date: 2026-03-01 DOI: 10.2478/acph-2026-0005

Ivana Mitrevska, Marina Chachorovska, Gjorgji Petrushevski

{"title":"Predictive analytical workflow for rapid structure elucidation and in silico toxicological qualification of an unidentified impurity in cefixime granules for oral suspension.","authors":"Ivana Mitrevska, Marina Chachorovska, Gjorgji Petrushevski","doi":"10.2478/acph-2026-0005","DOIUrl":"10.2478/acph-2026-0005","url":null,"abstract":"During in-use stability testing of cefixime granules for oral suspension, an impurity with a relative retention time of 0.19 was consistently detected and increased during storage. To ensure regulatory compliance and patient safety, the impurity was structurally identified and toxicologically qualified. A laboratory-scale formulation and commercial products were studied under refrigerated and ambient conditions. The impurity was isolated by automated fraction collection and characterised by liquid chromatography-mass spectrometry and tandem mass spectrometry. Kinetic evaluation showed pseudo-first-order formation, with faster accumulation at ambient temperature. The impurity was identified as a γ-lactone degradation product of cefixime, present as multiple stereoisomers stabilised under acidic conditions. In silico toxicological assessment using complementary platforms indicated no additional structural alerts, no mutagenic potential, and negligible acute toxicity. The impurity forms only after prolonged storage of reconstituted suspensions and is classified as an ICH M7 Class 5 impurity, requiring no further genotoxicity testing. The applied analytical-computational workflow provides an efficient approach for impurity qualification in β-lactam antibiotics.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":"76 1","pages":"1-25"},"PeriodicalIF":1.8,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pan-Cdk inhibitor ZK304709 suppresses Cdc20 expression and potentiates the anticancer activity of apcin in HeLa cervical cancer cells. Pan-Cdk抑制剂ZK304709抑制HeLa宫颈癌细胞中Cdc20的表达，增强apcin的抗癌活性。

IF 1.4 4区医学

Acta Pharmaceutica Pub Date : 2026-03-25 Print Date: 2026-03-01 DOI: 10.2478/acph-2026-0002

Xiangyang Le, Qingsong Chen, Shuyang Cao, Gaoyun Hu, Qianbin Li, Zhuo Chen

{"title":"Pan-Cdk inhibitor ZK304709 suppresses Cdc20 expression and potentiates the anticancer activity of apcin in HeLa cervical cancer cells.","authors":"Xiangyang Le, Qingsong Chen, Shuyang Cao, Gaoyun Hu, Qianbin Li, Zhuo Chen","doi":"10.2478/acph-2026-0002","DOIUrl":"10.2478/acph-2026-0002","url":null,"abstract":"Cell division cycle 20 homologue (Cdc20), a key regulator of the anaphase-promoting complex/cyclosome (APC/C), is frequently overexpressed in human cancers and represents a promising therapeutic target. However, monotherapy targeting Cdc20 has shown limited efficacy, partly due to compensatory activation of cyclin-dependent kinase 1 (Cdk1). In this study, we investigated the combinatorial potential of the pan-Cdk inhibitor ZK304709 with the Cdc20 inhibitor apcin in HeLa cervical cancer cells. Transcriptomic analysis revealed that both CDC20 and CDK1 are upregulated in cervical cancer tissues. Mechanistically, apcin treatment induced cyclin B1 accumulation and enhanced Cdk1 phosphorylation at Thr161, suggesting feedback activation. In contrast, ZK304709 reduced p-Cdk1(T161) levels and suppressed Cdc20 expression at both protein and mRNA levels. Functionally, the combination of apcin and ZK304709 synergistically inhibited cell proliferation and induced G2/M phase arrest in HeLa cells. These findings demonstrate that dual inhibition of Cdk1 and Cdc20 disrupts compensatory signalling pathways and enhances antitumour efficacy in HeLa cells, providing a rational strategy for combination therapy in cervical cancer.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":" ","pages":"1-13"},"PeriodicalIF":1.4,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145779710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative biodistribution analysis of umbilical cord mesenchymal stromal cells via different administration routes in rabbit models. 不同给药途径对兔脐带间充质间质细胞生物分布的比较分析。

IF 1.4 4区医学

Acta Pharmaceutica Pub Date : 2026-03-25 Print Date: 2026-03-01 DOI: 10.2478/acph-2026-0004

Liang Xiao, Chunlin Liu, Yuqian Wang, Fucheng Xiao, Jiahua Cai, Shoukang Qu, Xiaojuan Xu, Xiaoliang Wang, Yue Liu, Yutao Peng, Jia Liu

{"title":"Comparative biodistribution analysis of umbilical cord mesenchymal stromal cells via different administration routes in rabbit models.","authors":"Liang Xiao, Chunlin Liu, Yuqian Wang, Fucheng Xiao, Jiahua Cai, Shoukang Qu, Xiaojuan Xu, Xiaoliang Wang, Yue Liu, Yutao Peng, Jia Liu","doi":"10.2478/acph-2026-0004","DOIUrl":"10.2478/acph-2026-0004","url":null,"abstract":"Umbilical cord mesenchymal stem cells (UC-MSCs) have shown therapeutic potential in renal diseases due to their homing ability. This study compared the effects of three administration routes (intravenous, local renal injection, and interventional injection) on UC-MSC distribution in kidney tissue. Eighteen New Zealand rabbits were assigned to the three groups (n = 6 each), and DiI-labelled UC-MSCs were tracked using confocal microscopy to evaluate their distribution in the kidney, lung, and brain. Local renal injection led to high MSC concentrations at the injection site, but distribution to the contralateral kidney was minimal and comparable to that of intravenous injection. Intravenous delivery via the marginal ear vein was simple and convenient but resulted in limited renal homing (< 1 %) and no significant difference between kidneys. Interventional injection achieved the highest delivery efficiency (12.4 %) and a more uniform renal distribution. Notably, inflammatory cytokine levels (IL-6, TNF-α, IL-10) were significantly elevated in the local injection group (p < 0.05). These results indicated that the choice of administration route critically affects MSC targeting and therapeutic potential, and interventional injection may offer the most effective strategy for precise UC-MSC delivery in renal therapy.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":" ","pages":"1-17"},"PeriodicalIF":1.4,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145909976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of green analytical principles in the HPLC analysis of prednisolone derivatives: Method optimization and validation for nasal powder formulations. 绿色分析原理在强的松龙衍生物高效液相色谱分析中的应用：鼻用粉剂配方的方法优化与验证。

IF 1.8 4区医学

Acta Pharmaceutica Pub Date : 2026-01-13 Print Date: 2025-12-01 DOI: 10.2478/acph-2025-0032

Daniela Amidžić Klarić, Jelena Kovačić, Ilija Klarić, Petra Bajt, Lana Pribolšan, Erika Križanić, Laura Nižić Nodilo, Ana Mornar

{"title":"Application of green analytical principles in the HPLC analysis of prednisolone derivatives: Method optimization and validation for nasal powder formulations.","authors":"Daniela Amidžić Klarić, Jelena Kovačić, Ilija Klarić, Petra Bajt, Lana Pribolšan, Erika Križanić, Laura Nižić Nodilo, Ana Mornar","doi":"10.2478/acph-2025-0032","DOIUrl":"10.2478/acph-2025-0032","url":null,"abstract":"Glucocorticoids are a group of drugs increasingly used in modern medical practice due to their pronounced anti--inflammatory and immunosuppressive properties. In this study, prednisolone disodium phosphate and prednisolone acetate were analysed, with the aim of developing and validating an HPLC method in accordance with ICH Q2(R2) guidelines and quantifying their content in the active pharmaceutical ingredient powder and a model in--house sample. By applying the HPLC-DAD method with gradient elution, effective separation of the analytes was achieved. The method met all validation parameter requirements. The obtained results showed that the content of both analytes in the tested samples (bulk API powders and in-house prepared model formulation) was within the prescribed limits according to current pharmacopoeial standards. The proposed HPLC-DAD method was assessed for its applicability and environmental profile utilizing a range of green and blue metric tools. This comprehensive evaluation confirms that the method adheres to green analytical principles, making it suitable for sustainable pharmaceutical analysis.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":"75 4","pages":"577-595"},"PeriodicalIF":1.8,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145958233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Double-blind randomised controlled trial of an emollient cream with and without 1 % supercritical CO₂ extract of Calendula officinalis in contact dermatitis. 含和不含1%金盏花超临界CO2提取物的润肤膏治疗接触性皮炎的双盲随机对照试验。

IF 1.8 4区医学

Acta Pharmaceutica Pub Date : 2026-01-13 Print Date: 2025-12-01 DOI: 10.2478/acph-2025-0035

Mia Šušak Crnčević, Toni Durdov, Doris Rušić, Lara Ramić, Ana Ćosić, Josipa Bukić, Stela Jokić, Krunoslav Aladić

{"title":"Double-blind randomised controlled trial of an emollient cream with and without 1 % supercritical CO2 extract of Calendula officinalis in contact dermatitis.","authors":"Mia Šušak Crnčević, Toni Durdov, Doris Rušić, Lara Ramić, Ana Ćosić, Josipa Bukić, Stela Jokić, Krunoslav Aladić","doi":"10.2478/acph-2025-0035","DOIUrl":"10.2478/acph-2025-0035","url":null,"abstract":"This double-blind randomised controlled trial aimed to evaluate the efficacy of an emollient cream with and without 1 % supercritical CO2 extract of Calendula officinalis in contact dermatitis. Twenty healthy volunteers without pre-existing dermatological conditions participated in this single-centre study. Each participant's forearm was divided into three test sites: control (no treatment), placebo (base emollient), and intervention (calendula cream). The study employed random allocation of test sites using Microsoft Excel software. Both investigators and participants were blinded to the treatment assignments. The main outcomes were changes in skin hydration and transepidermal water loss (TEWL), measured with non-invasive probes (Corneometer CM 825, Tewameter TM 300), and erythema measured by Mexameter MX 18. Following irritant exposure, all sites showed increased TEWL and reduced hydration, confirming skin barrier impairment. The intervention site demonstrated significantly greater hydration compared with both the control (p = 0.017) and placebo sites (p = 0.035) on day 4, with this improvement persisting on day 8 (p = 0.043). TEWL values at the intervention site were significantly lower than control on day 3 (p = 0.022), indicating faster barrier recovery. No significant differences in erythema were observed between groups, and no adverse events occurred. Results of this study indicate that the addition of 1 % Calendula officinalis extract to an emollient cream enhanced skin hydration and accelerated recovery after irritant exposure, suggesting potential benefit in managing contact dermatitis with non-pharmacological skincare formulations.","PeriodicalId":7034,"journal":{"name":"Acta Pharmaceutica","volume":"75 4","pages":"699-708"},"PeriodicalIF":1.8,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145958255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0