Phytochemical composition, antioxidant, antiglycation, and antihyperlipidemic activity of flowering parts from five plant species before and after in vitro digestion.

IF 2.1 4区 医学 Q3 PHARMACOLOGY & PHARMACY
Valerija Vujčić Bok, Domagoj Bosiljevac, Ivana Šola, Ana Vukres, Gordana Rusak, Željan Maleš
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引用次数: 0

Abstract

This study evaluates the antihyperlipidemic (pancreatic lipase inhibition assay), antiglycation (inhibition of bovine serum albumin glycation, BSA glycation), and antioxidant activity (ABTS, DPPH and FRAP assays) of ethanolic extracts from flowering parts of five widely distributed plant species in Croatia - Crocus heuffelianus Herb. (tepals), Nicotiana tabacum L. (petals), Malva sylvestris L. (petals), Calendula officinalis L. and Helianthus annuus L. (both sterile ligulate flowers). An in vitro-simulated system of human digestion was employed to assess the bioaccessibility of the selected phenolics and the stability of the extracts' antioxidant, hypolipidemic, and antiglycation potential following each digestion phase. The concentrations of l-ascorbic acid, individual flavonoids, and phenolic acids were determined using RP-HPLC analysis. Principal component analysis revealed significant differences in the content of bioactive compounds and their biological activity among the investigated plant species. All original extracts exhibited high antioxidant capacity (> 70 %) in at least one assay, with N. tabacum and H. annuus demonstrating the strongest antioxidant capacity throughout digestion. H. annuus contained the highest levels of total identified phenolic acids, total identified phenols, and total identified compounds, while N. tabacum and C. heuffelianus exhibited the highest total flavonoid content. Among individual compounds, protocatechuic acid, quercetin, and ferulic acid significantly contributed to antioxidant activity. N. tabacum had the strongest antihyperlipidemic potential in the original extracts, as well as in the most digestion phases. Strong BSA glycation inhibition (70-100 %) was observed in all plant extracts across various digestion phases, with the exception of C. heuffelianus, which exhibited moderate inhibitory effects. These findings suggest that the analyzed flower-derived plant materials, some of which are often considered agricultural waste, can serve as sustainable and valuable resources of bioactive compounds for functional food, dietary supplements, and pharmaceutical applications.

五种植物开花部位体外消化前后的植物化学成分、抗氧化、抗糖化和抗高血脂活性
本研究评估了克罗地亚五种广泛分布的植物——番红花(Crocus heuffelianus Herb)开花部位的乙醇提取物的抗高脂血症(胰脂肪酶抑制试验)、抗糖化(抑制牛血清白蛋白糖化、BSA糖化)和抗氧化活性(ABTS、DPPH和FRAP试验)。(花被片),烟草(花瓣),金盏花(花瓣),金盏菊(金盏菊)和向日葵(金盏菊)(都是不育舌状花)。采用体外模拟人体消化系统来评估所选酚类物质的生物可及性以及提取物在每个消化阶段的抗氧化、降血脂和抗糖化电位的稳定性。采用反相高效液相色谱法测定l-抗坏血酸、黄酮类化合物和酚酸的浓度。主成分分析表明,不同植物的活性成分含量和活性存在显著差异。在至少一项试验中,所有原始提取物都显示出较高的抗氧化能力(bbb70 %),其中烟草和黄花荆芥在整个消化过程中表现出最强的抗氧化能力。黄杨的总酚酸、总酚类物质和总化合物含量最高,而烟叶和黄酮含量最高。在单个化合物中,原儿茶酸、槲皮素和阿魏酸对抗氧化活性有显著贡献。在原始提取物中,以及在大多数消化阶段,烟草具有最强的抗高脂血症潜力。除C. heuffelianus表现出中等抑制作用外,所有植物提取物在不同消化阶段均表现出较强的BSA糖基化抑制作用(70- 100%)。这些发现表明,所分析的花源性植物材料,其中一些通常被认为是农业废物,可以作为功能性食品、膳食补充剂和制药应用的生物活性化合物的可持续和宝贵资源。
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来源期刊
Acta Pharmaceutica
Acta Pharmaceutica PHARMACOLOGY & PHARMACY-
CiteScore
5.20
自引率
3.60%
发文量
20
审稿时长
>12 weeks
期刊介绍: AP is an international, multidisciplinary journal devoted to pharmaceutical and allied sciences and contains articles predominantly on core biomedical and health subjects. The aim of AP is to increase the impact of pharmaceutical research in academia, industry and laboratories. With strong emphasis on quality and originality, AP publishes reports from the discovery of a drug up to clinical practice. Topics covered are: analytics, biochemistry, biopharmaceutics, biotechnology, cell biology, cell cultures, clinical pharmacy, drug design, drug delivery, drug disposition, drug stability, gene technology, medicine (including diagnostics and therapy), medicinal chemistry, metabolism, molecular modeling, pharmacology (clinical and animal), peptide and protein chemistry, pharmacognosy, pharmacoepidemiology, pharmacoeconomics, pharmacodynamics and pharmacokinetics, protein design, radiopharmaceuticals, and toxicology.
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