Acta neurobiologiae experimentalis最新文献_第7页

Hydrogen‑rich saline promotes neuronal recovery in mice with cerebral ischemia through the AMPK/mTOR signal‑mediated autophagy pathway. 富氢盐水通过AMPK/mTOR信号介导的自噬途径促进脑缺血小鼠的神经元恢复。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2023-09-29 DOI: 10.55782/ane-2023-2437

Jing Wang, Xiang-Jian Zhang, Yuan-Yuan Du, Guang Shi, Cong-Cong Zhang, Rong Chen

{"title":"Hydrogen‑rich saline promotes neuronal recovery in mice with cerebral ischemia through the AMPK/mTOR signal‑mediated autophagy pathway.","authors":"Jing Wang, Xiang-Jian Zhang, Yuan-Yuan Du, Guang Shi, Cong-Cong Zhang, Rong Chen","doi":"10.55782/ane-2023-2437","DOIUrl":"10.55782/ane-2023-2437","url":null,"abstract":"This study explored the protective effect and mechanism of hydrogen‑rich saline (HRS) on the neurological function of mice with cerebral ischemia. Effects of HRS on neurological function in mice with cerebral ischemia were evaluated by neurological function scores. Infarct volume and histological damage were evaluated by 2,3,5‑triphenyl tetrazolium chloride staining (TTC staining). Golgi‑Cox staining was conducted to measure the morphological changes of neuronal dendrites and dendritic spines. The expression of neuronal markers was detected by immunofluorescence. Western blot was used to detect protein expression. The infarct volume of mice in the HRS‑H group decreased significantly compared to that of the distal middle cerebral artery occlusion (dMCAO) group. Mice in the HRS‑H group had a lower neurological deficit score than that in the dMCAO group. Compared to the dMCAO group, the activity of superoxide dismutase (SOD) and the level of glutathione (GSH) significantly increased in the HRS‑H group. Compared with the dMCAO group, the number of apoptotic cells in the HRS‑H group decreased. Administration of HRS was shown to be able to decrease cavitation of the brain cortex after ischemia. The spine density in the HRS‑H group increased compared to that of the dMCAO group. In the in vitro experiment, compared with the oxygen‑glucose deprivation (OGD) group, the active oxygen content in the 75% HRM group decreased, and the mitochondrial membrane potential and adenosine triphosphate (ATP) content increased. Compared with the OGD group, the ratio of P‑AMPK and the levels of LC3II/LC3I in the hydrogen‑rich medium (HRM) group was upregulated, and P‑mTOR levels and P62 levels in the HRM group were down‑regulated. HRS can enhance neuroplasticity after ischemia and promote neurological recovery in mice with cerebral ischemia, which may involve the autophagy pathway mediated by the AMPK/mTOR signaling pathway.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"83 3","pages":"317-330"},"PeriodicalIF":1.4,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49688304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of vitamin D on mood alteration in women's life: Focus on depression. 维生素D对女性生活中情绪变化的影响：关注抑郁症。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2023-09-29 DOI: 10.55782/ane-2023-2436

Isidoros Mentis

{"title":"The effects of vitamin D on mood alteration in women's life: Focus on depression.","authors":"Isidoros Mentis","doi":"10.55782/ane-2023-2436","DOIUrl":"10.55782/ane-2023-2436","url":null,"abstract":"Vitamin D (VD) is a vital liposoluble neurosteroid micronutrient, particularly crucial for women's health. International literature strongly correlates sufficient VD levels with comprehensive mental well‑being in women. This link is intricately related to neurobiological pathways and hormonal fluctuations, where low VD levels are notably associated with depression. This study comprehensively explores the neurobiological mechanisms that link VD and altered mood in women. Considering the increased susceptibility to hormonal shifts in women, our research investigates the intricate interplay between VD's neurobiology and mood regulation. Through the focused analysis of specific studies, we untangle the complex web of connections between VD and mood changes in women. Our approach takes into account the dynamic nature of hormonal changes, deepening our understanding of these mechanisms. Our study underscores VD's significant role as a neurosteroid micronutrient, especially in women's health. By examining the intricate relationships between VD's neurobiology and hormones, we propose strategies to improve mood regulation and psychological well‑being in women. In addition, we recommend targeted measures to achieve optimal VD levels, helping to manage challenges arising from hormonal fluctuations. The present review highlights the multifaceted contribution of VD to women's health, particularly in mood regulation. Through the analysis of the interplay of neurobiology, hormones and VD, our study provides avenues for enhancing women's mental and emotional well‑being through customized interventions.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"83 3","pages":"307-316"},"PeriodicalIF":1.4,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49688311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential approach to stroke aphasia and primary progressive aphasia using transcranial magnetic stimulation: A systematic review. 经颅磁刺激治疗脑卒中失语症和原发性进行性失语症的鉴别方法：一项系统综述。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2023-09-29 DOI: 10.55782/ane-2023-2433

Verónica Pérez-Martínez, Candela Zorzo, Marta Méndez

{"title":"Differential approach to stroke aphasia and primary progressive aphasia using transcranial magnetic stimulation: A systematic review.","authors":"Verónica Pérez-Martínez, Candela Zorzo, Marta Méndez","doi":"10.55782/ane-2023-2433","DOIUrl":"10.55782/ane-2023-2433","url":null,"abstract":"Language disorders can occur as a consequence of stroke or neurodegenerative disorders, among other causes. Post‑stroke aphasia (PSA) and primary progressive aphasia (PPA) are syndromes that, despite having common features, differ in the brain mechanisms that cause their symptoms. These differences in the underlying functional neuroanatomical changes may influence the way they are addressed by different non‑invasive brain stimulation techniques and, in particular, by repetitive transcranial magnetic stimulation (rTMS). The aim of this systematic review is to evaluate the efficacy of rTMS in the treatment of PSA and PPA, as well as the differences in the approach to these disorders using rTMS. To this end, a total of 36 articles were found in the Web of Science, Scopus, and PubMed. The results obtained suggest that whereas in PSA, the selection of the stimulation paradigm is based on bi‑hemispheric functional reorganisation models with a tendency towards the application of inhibitory rTMS in the contralateral right hemisphere, in PPA, the application of excitatory rTMS in functionally compromised areas seems to show promising changes. It is concluded that rTMS is a potential treatment in the therapy of both disorders, although differences in the underlying brain mechanisms differentiate the rTMS approach in each case.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"83 3","pages":"280-298"},"PeriodicalIF":1.4,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49688303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuroprotective effect of 1,25‑dihydroxyvitamin D3 against hyperoxia‑induced brain injury in premature rats. 1,25-二羟基维生素D3对早产大鼠高氧诱导的脑损伤的神经保护作用。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2023-09-29 DOI: 10.55782/ane-2023-2435

Rong Chen, Fahua Yao, Xiaodan Deng, Xiaofeng Yuan, Nian Wei, Dongfan Xiao, Benli Yu

{"title":"Neuroprotective effect of 1,25‑dihydroxyvitamin D3 against hyperoxia‑induced brain injury in premature rats.","authors":"Rong Chen, Fahua Yao, Xiaodan Deng, Xiaofeng Yuan, Nian Wei, Dongfan Xiao, Benli Yu","doi":"10.55782/ane-2023-2435","DOIUrl":"10.55782/ane-2023-2435","url":null,"abstract":"Studies have shown that vitamin D plays a crucial role in brain development, brain metabolism and neuroprotection. There is little evidence for the neuroprotective effect of 1, 25‑dihydroxyvitamin D3 (1,25‑(OH)2D3) on various brain injury models. The aim of this study was to investigate the neuroprotection effect of 1,25‑(OH)2D3 against hyperoxia‑induced brain injury in premature rats. Sprague‑Dawley rats were exposed to 95% oxygen or room air for 24 h and treated with 1,25‑(OH)2D3 or normal saline for 14 consecutive days. The histopathological changes of optic chiasma tissue were observed by hematoxylin‑eosin staining. Immunohistochemistry, qRT‑PCR, and western blot were performed to detect the expression of integrin‑β1 and yes‑associated protein (YAP) in the organization of the optic chiasm. Histopathological sections of optic chiasma showed visible optic nerve swelling, expanded nerve fiber space, uneven staining, obvious oligodendrocyte proliferation and disordered cell arrangement accompanied by inflammatory cell infiltration and exudation after 7 days and 14 days of hyperoxia exposure. The hyperoxia group treated with 1,25‑(OH)2D3 were showed improvement of brain injury with reduced inflammatory exudation, uniform nerve fiber staining and less obvious oligodendrocyte proliferation. Immunohistochemical staining, qRT‑PCR and western blot indicated that 1,25‑(OH)2D3 treatment upregulated the expression of integrin‑β1 and YAP in the hyperoxia group on day 7. However, the expression of YAP was significantly increased compared with control group and treatment with 1,25‑(OH)2D3 reduced the expression of YAP in the hyperoxic group on day 14. 1,25‑(OH)2D3 may regulate the expression of integrin‑β1 and YAP to alleviate hyperoxia‑induced brain injury in premature rats.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"83 3","pages":"299-306"},"PeriodicalIF":1.4,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49688308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of bee venom on behavior and the role of leptin in rats. 蜂毒对大鼠行为的影响及瘦素的作用。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2023-09-29 DOI: 10.55782/ane-2023-2430

Bahar Dalkiran, Burcu Acikgoz, Ilkay Aksu, Amac Kiray, Muge Kiray

{"title":"The effects of bee venom on behavior and the role of leptin in rats.","authors":"Bahar Dalkiran, Burcu Acikgoz, Ilkay Aksu, Amac Kiray, Muge Kiray","doi":"10.55782/ane-2023-2430","DOIUrl":"10.55782/ane-2023-2430","url":null,"abstract":"The aim of this study is to evaluate the dose‑dependent effect of bee venom (BV) on behavioral functions in rats and the physiological role of leptin in the prefrontal cortex, hippocampus, and amygdala tissues. Adult Sprague‑Dawley male rats were used in the experiments. The rats were divided into three groups of control, 0.1 mg/kg BV, and 0.5 mg/kg BV. The rats were injected with BV subcutaneously for 15 consecutive days. The open field test (OFT), the elevated plus maze test (EPM), and the forced swimming test (FST) were performed as behavioral assessments. Animals were sacrificed, and brain regions were removed. Leptin levels were measured in various brain regions by ELISA. In the OFT, the total distance and speed for the 0.1 mg/kg BV group increased compared to controls and the 0.5 mg/kg BV group. In the EPM, the 0.1 mg/kg BV group remained in the open arm for a significantly longer period of time compared to the other groups. In the FST, the 0.5 mg/kg BV group was more mobile than the other groups. Leptin levels in the prefrontal cortex were significantly higher in the 0.1 mg/kg BV group compared to the control and 0.5 mg/kg groups. There were no significant differences between groups in hippocampus and amygdala leptin levels. The results of the study show that BV has a positive effect on behavioral parameters. BV may have a positive effect on anxiety‑ and depression‑like behaviors by increasing leptin levels in the prefrontal cortex.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"83 3","pages":"255-261"},"PeriodicalIF":1.4,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49688310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The paraventricular nucleus of the hypothalamus - the concertmaster of autonomic control. Focus on blood pressure regulation. 下丘脑室旁核——自主神经控制的大师。关注血压调节。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2023-01-01 DOI: 10.55782/ane-2023-004

Emilia Grzęda, Kamil Ziarniak, Joanna H Sliwowska

{"title":"The paraventricular nucleus of the hypothalamus - the concertmaster of autonomic control. Focus on blood pressure regulation.","authors":"Emilia Grzęda, Kamil Ziarniak, Joanna H Sliwowska","doi":"10.55782/ane-2023-004","DOIUrl":"https://doi.org/10.55782/ane-2023-004","url":null,"abstract":"The autonomic nervous system regulates internal organs and peripheral circulation, which enables the maintenance of homeostasis in vertebrate species. One of the brain regions involved in autonomic and endocrine homeostasis regulation is the paraventricular nucleus of the hypothalamus (PVN). The PVN is a unique site at which multiple input signals can be assessed and integrated. The regulation of the autonomic system by the PVN and, especially, the sympathetic flow, depends upon the integration of inhibitory and excitatory neurotransmitter action. The excitatory neurotransmitters such as glutamate and angiotensin II, and inhibitory neurotransmitters such as γ‑aminobutyric acid and nitric oxide, play a key role in the physiological function of the PVN. Moreover, arginine-vasopressin (AVP) and oxytocin (OXT) are important in the regulation of sympathetic system activity. The PVN is also crucial for maintaining cardiovascular regulation, with its integrity being pivotal for blood pressure regulation. Studies have shown that pre‑autonomic sympathetic PVN neurons increase blood pressure and the dysfunction of these neurons is directly related to elevated sympathetic nervous system activity under hypertension. Etiology of hypertension in patients is not fully known. Thus, understanding the role of PVN in the generation of hypertension may help to treat this cardiovascular disease. This review focuses on the PVN's inhibitory and excitatory neurotransmitter interactions that regulate sympathetic system activity in physiological conditions and hypertension.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"83 1","pages":"34-44"},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9384494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Sensory‑motor performance and neurochemical effects in the cerebral cortex of brain‑derived neurotrophic factor heterozygous mice fed a high‑cholesterol diet. 饲喂高胆固醇饮食的脑源性神经营养因子杂合小鼠大脑皮层的感觉运动表现和神经化学效应。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2023-01-01 DOI: 10.55782/ane-2023-001

Hatice Keser, Ozlem Bozkurt Girit, Selcen Aydin Abidin, Mehmet Dincer Bilgin, Ahmet Alver, İsmail Abidin

{"title":"Sensory‑motor performance and neurochemical effects in the cerebral cortex of brain‑derived neurotrophic factor heterozygous mice fed a high‑cholesterol diet.","authors":"Hatice Keser, Ozlem Bozkurt Girit, Selcen Aydin Abidin, Mehmet Dincer Bilgin, Ahmet Alver, İsmail Abidin","doi":"10.55782/ane-2023-001","DOIUrl":"https://doi.org/10.55782/ane-2023-001","url":null,"abstract":"Reports suggest that a high‑cholesterol diet may induce neuroinflammation, oxidative stress, and neurodegeneration in brain tissue. Brain‑derived neurotrophic factor (BDNF) might play a role in protecting against changes induced by high cholesterol. We aimed to assess behavioral correlates and biochemical alterations in the motor and sensory cortices following a high‑cholesterol diet under normal and reduced BDNF concentrations. C57Bl/6 strain, wild‑type (WT) and BDNF heterozygous (+/‑) mice were used to reveal the effects of endogenous BDNF concentrations. We compared diet and genotype effects using four experimental groups: WT and BDNF heterozygous (+/‑) groups of mice were each fed a normal or high‑cholesterol diet for 16 weeks. The cylinder test and wire hanging test were performed to evaluate neuromuscular deficits and cortical sensory‑motor functions, respectively. In addition, neuroinflammation was assessed by tumor necrosis factor alpha and interleukin 6 levels measured in the somatosensory and motor areas. Additionally, MDA levels and SOD and CAT activity were evaluated as oxidative stress parameters. Results showed that a high‑cholesterol diet significantly impaired behavioral performance in the BDNF (+/‑) group. Diet did not change the levels of neuroinflammatory markers in any of the groups. However, MDA levels, an indicator of lipid peroxidation, were significantly higher in the high‑cholesterol‑fed BDNF (+/‑) mice. The results suggest that BDNF levels might be a critical factor in determining the extent of neuronal damage induced in the neocortex by a high‑cholesterol diet.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"83 1","pages":"1-9"},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9384496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential local field potential oscillations in the dorsal striatum and locomotor activity induced by morphine and haloperidol in mice. 吗啡和氟哌啶醇诱导小鼠背纹状体局部场电位的差异振荡和运动活动。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2023-01-01 DOI: 10.55782/ane-2023-013

Chayaporn Reakkamnuan, Dania Cheaha, Nifareeda Samerphop, Jakkrit Nukitram, Ekkasit Kumarnsit

{"title":"Differential local field potential oscillations in the dorsal striatum and locomotor activity induced by morphine and haloperidol in mice.","authors":"Chayaporn Reakkamnuan, Dania Cheaha, Nifareeda Samerphop, Jakkrit Nukitram, Ekkasit Kumarnsit","doi":"10.55782/ane-2023-013","DOIUrl":"https://doi.org/10.55782/ane-2023-013","url":null,"abstract":"Dopamine (DA) depletion in the dorsal striatum underlies symptoms of basal ganglia pathologies, including Parkinson's disease (PD). Various drug compounds are used to enhance DA levels for therapeutic purposes. Understanding neural signaling and movement patterns associated with over‑ and under‑stimulation of the DA system is essential. This study investigated striatal local field potential (LFP) oscillation and locomotor activity following treatments with morphine, a DA release enhancer, and haloperidol (HAL), a DA D2 receptor (D2R) antagonist in mice. After intracranial electrodes were placed into the dorsal striatum of male Swiss albino ICR mice, intraperitoneal injections of morphine or HAL were administered. LFP signals and spontaneous motor activity were recorded simultaneously. The results showed that morphine significantly increased locomotor speed, both low (30.3-44.9 Hz) and high (60.5-95.7 Hz) LFP gamma powers and delta (1-4 Hz)‑gamma (30.3-95.7 Hz) phase‑amplitude coupling. In contrast, HAL treatments were found to significantly decrease these parameters. Moreover, regression analyses also revealed significant positive correlations between locomotor speed and high gamma powers. Taken together, these results demonstrate opposite LFP oscillations in the dorsal striatum with low and high gamma activities, and delta‑gamma couplings in response to a DA release enhancer and D2R antagonist by morphine and HAL, respectively. These parameters reflect fluctuation of neuronal activity in the dorsal striatum that might be useful for pathological research and drug discovery for PD.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"83 2","pages":"140-153"},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9866989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sub‑chronic administration of lead alters markers of oxidative stress, acetylcholinesterase and Na+K+‑ATPase activities in rat brain. 亚慢性给药可改变大鼠脑内氧化应激标志物、乙酰胆碱酯酶和Na+K+ atp酶活性。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2023-01-01 DOI: 10.55782/ane-2023-019

Magda Helena Soratto Heitich Ferrazza, Débora Delwing-Dal Magro, Eloise Salamaia, Thales Ercole Guareschi, Luis Felipe Fernandes Erzinger, Thayná Patachini Maia, Cassiana Siebert, Tiago Marcon Dos Santos, Angela Terezinha de Souza Wyse, Gabriela Borgmann, Katherine Plautz, Daniela Delwing-de Lima

{"title":"Sub‑chronic administration of lead alters markers of oxidative stress, acetylcholinesterase and Na+K+‑ATPase activities in rat brain.","authors":"Magda Helena Soratto Heitich Ferrazza, Débora Delwing-Dal Magro, Eloise Salamaia, Thales Ercole Guareschi, Luis Felipe Fernandes Erzinger, Thayná Patachini Maia, Cassiana Siebert, Tiago Marcon Dos Santos, Angela Terezinha de Souza Wyse, Gabriela Borgmann, Katherine Plautz, Daniela Delwing-de Lima","doi":"10.55782/ane-2023-019","DOIUrl":"https://doi.org/10.55782/ane-2023-019","url":null,"abstract":"This study investigated the effects of sub‑chronic administration of lead (Pb) acetate on thiobarbituric acid reactive substances (TBA‑RS), total sulfhydryl content, protein carbonyl content, antioxidant enzymes (superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GSH‑Px]), acetylcholinesterase (AChE), and Na+K+‑ATPase in the cerebral structures of rats. Male Wistar rats aged 60 days were treated with saline (control group) or Pb (treatment group), at various doses, by gavage, once a day for 35 days. The animals were sacrificed twelve hours after the last administration, and the cerebellum, hippocampus and cerebral cortex were removed. The results showed that Pb did not alter the evaluated oxidative stress parameters. Furthermore, Pb (64 and/or 128 mg/kg) altered SOD in the cerebellum, cerebral cortex and hippocampus. Pb (128 mg/kg) altered CAT in the cerebellum and cerebral cortex and GSH‑Px in the cerebral cortex. Also, Pb (64 mg/kg and 128 mg/kg) altered GSH‑Px in the cerebellum. Moreover, Pb (128 mg/kg) increased AChE in the hippocampus and decreased Na+K+‑ATPase in the cerebellum and hippocampus. In conclusion, sub‑chronic exposure to Pb (occupational and environmental intoxication) altered antioxidant enzymes, AChE, and Na+K+‑ATPase, contributing to cerebral dysfunction.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"83 2","pages":"216-225"},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9875744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The enriched environment prevents degeneration of cerebellum Purkinje cells layer of rats. 富营养化环境可防止大鼠小脑浦肯野细胞层变性。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2023-01-01 DOI: 10.55782/ane-2023-015

Josiane Mann, Valter Malaguido Clímaco, Seigo Nagashima, Daniel Wolff Stanczyk, Melania Santer, Ilton Silva, Luiz Fernando Pereira

{"title":"The enriched environment prevents degeneration of cerebellum Purkinje cells layer of rats.","authors":"Josiane Mann, Valter Malaguido Clímaco, Seigo Nagashima, Daniel Wolff Stanczyk, Melania Santer, Ilton Silva, Luiz Fernando Pereira","doi":"10.55782/ane-2023-015","DOIUrl":"https://doi.org/10.55782/ane-2023-015","url":null,"abstract":"Neurodegeneration is characterized by loss of neurons causing changes that lead individuals to debilitating conditions; the most common of this condition is the Alzheimer's disease. It has been related that enriched environment (EE) induces experience‑dependent plasticity mechanisms, improving the performance of the animals in learning and memory tests. This study evaluated the effects of EE on histological parameters of the cerebellum in rats that received intracerebroventricular streptozotocin. In the standard environment, streptozotocin (STZ) promoted a significant increase between the gaps in the Purkinje layer of approximately 20%. On the other hand, in an enriched environment, the control result (EE) was similar to the result under streptozotocin effect (STZEE). In the standard environment (SE) group a 26% significant reduction in Purkinje cell density was observed under STZ presence. By analyzing the results of the density of Purkinje cells under the effect of streptozotocin in a standard environment (STZSE) against the density of the layer of Purkinje cells also under the effect of streptozotocin in an enriched environment (STZEE), a significant reduction of approximately 76% in density was observed of Purkinje cells in standard environment (STZSE), the mean number of Purkinje cells in enriched environments was not reduced, despite of STZ. According to the results, treatment with STZ and exposure to EE did not change the cerebellum general morphology/cytoarchitecture, hence was no significant difference in the layers thickness. These facts demonstrate that the enriched environment appears to protect the Purkinje cells layer of cerebellum from possible degeneration.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"83 2","pages":"171-178"},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9875739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0