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Is it still adequate to study the nervous system using methods of catalytic enzyme histochemistry? 用催化酶组织化学的方法来研究神经系统是否还足够?
Acta histochemica. Supplementband Pub Date : 1992-01-01
R Gossrau, W Richter
{"title":"Is it still adequate to study the nervous system using methods of catalytic enzyme histochemistry?","authors":"R Gossrau,&nbsp;W Richter","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The frequent use of methods different from enzyme histochemistry evokes the question, whether it is still useful to apply methods of catalytic enzyme histochemistry to study the nervous system. In this brief overview it is shown, that catalytic enzyme histochemistry can still contribute to a better understanding of nervous system function. This was enabled by methodological progress, i.e., the modification of already existing procedures or the development of new techniques for the visualization and measurement of enzymes in tissue sections using their catalytic properties. The methods for acetylcholinesterase, monoamine oxidase as well as certain exoglycosidases and phosphatases will be given as examples. The application of these procedures as well as of methods for proteases, oxyradical-generating oxidases and enzymes involved in the metabolism of amino acid and other transmitters will illustrate, that qualitative (localization) and quantitative (measurement) catalytic enzyme histochemistry can still contribute to a better understanding of nervous system function.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"39-49"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12523547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Preoperative computer tomography and postoperative classification of brain tumors]. [术前计算机断层扫描及术后脑肿瘤分类]。
Acta histochemica. Supplementband Pub Date : 1992-01-01
D Schreiber, H Pothe, H Assmann, J Schneider
{"title":"[Preoperative computer tomography and postoperative classification of brain tumors].","authors":"D Schreiber,&nbsp;H Pothe,&nbsp;H Assmann,&nbsp;J Schneider","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Imaging of different brain tumor types by computed tomography (CT) or contrast-enhanced CT scans is often very similar. Therefore, the exact preoperative CT diagnosis of intracranial neoplasms is difficult. Among 100 cases (88 primary brain tumors, 12 brain metastases), the preoperative classification by CT was correct in 51 and partially correct in 22 cases. A corresponding presumptive CT diagnosis was made in 22 brain neoplasms. 5 cases were misinterpreted. Examples of CT scans and histological pictures are compared and analysed according to the literature.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"185-93"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12751930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluorescent latex microspheres for retrograde tracing of neurons in mouse basal forebrain combined with immunocytochemistry: a methodical approach. 荧光乳胶微球结合免疫细胞化学逆行示踪小鼠基底前脑神经元:一种系统的方法。
Acta histochemica. Supplementband Pub Date : 1992-01-01
W Härtig, B R Paulke, G Brückner
{"title":"Fluorescent latex microspheres for retrograde tracing of neurons in mouse basal forebrain combined with immunocytochemistry: a methodical approach.","authors":"W Härtig,&nbsp;B R Paulke,&nbsp;G Brückner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The presented methodical contribution demonstrates the suitability of rhodamine-labelled latex microspheres with a defined mean diameter of around 20 nm for retrograde neuronal tracing. After small injections of about 200 nl red fluorescent tracer into visual cortex of mice afferent neurons were labelled in cortical and subcortical structures. Basal forebrain neurons containing the tracer were further characterized by the concurrent visualization of choline acetyltransferase, a marker for cholinergic neurons, and parvalbumin, a putative marker of GABAergic neurons, by immunofluorescence.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"261-5"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12752630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The histochemical formation of the myoadenylate deaminase reaction in human skeletal musculature]. [人骨骼肌组织中肌腺苷酸脱氨酶反应的组织化学形成]。
Acta histochemica. Supplementband Pub Date : 1992-01-01
V Herrmann
{"title":"[The histochemical formation of the myoadenylate deaminase reaction in human skeletal musculature].","authors":"V Herrmann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Muscle biopsies from control subjects, patients with neuromuscular diseases and premature infants and neonates were investigated for myoadenylate deaminase activity (MAD) by histochemistry. A histochemically picture of MAD-deficiency is more frequently then the clinically defined MAD-deficiency syndrome with an autosomal recessive pattern of inheritance. The high incidence of the carrier state, secondary MAD-deficiency and connections with other diseases are the causes. An individual predisposition for loading crisis of these patients in a high physical stress situation is probable.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"325-31"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12752638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Histochemical representation of acetylcholinesterase in Alzheimer's disease]. [阿尔茨海默病中乙酰胆碱酯酶的组织化学表征]。
Acta histochemica. Supplementband Pub Date : 1992-01-01
J Ulrich
{"title":"[Histochemical representation of acetylcholinesterase in Alzheimer's disease].","authors":"J Ulrich","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Senile plaques in the cerebral neocortex, entorhinal cortex and hippocampus are reactive for Acetylcholinesterase (AChE). The same types of plaques are observed, as with immunostains for beta-protein, including the very simple ones, consisting nearly exclusively of loose deposits of beta-protein. If there are many plaques, the normal network of AChE-positive axons disappears. Explanations for both, the apparent shift of AChE from the axons to the plaques on the one hand and the very early development of AChE positive deposits during the plaque development on the other hand are sought.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"11-2"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12548055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid development of giant aneurysm at the base of the brain in an 8-year-old boy with perinatal HIV infection. 一名8岁围生期HIV感染男孩脑底部快速发展的巨大动脉瘤。
Acta histochemica. Supplementband Pub Date : 1992-01-01
C Lang, G Jacobi, W Kreuz, H Hacker, G Herrmann, H G Keul, E Thomas
{"title":"Rapid development of giant aneurysm at the base of the brain in an 8-year-old boy with perinatal HIV infection.","authors":"C Lang,&nbsp;G Jacobi,&nbsp;W Kreuz,&nbsp;H Hacker,&nbsp;G Herrmann,&nbsp;H G Keul,&nbsp;E Thomas","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An 8-year-old boy with perinatal HIV infection developed a large fusiform aneurysm in the circle of Willis two years prior to death which was confirmed by radiological studies. The postmortem examinations revealed a predominantly intimal, proliferative lesion, and partial destruction of the internal elastic lamina in the involved arteries. Within the intima hyperplasia of fibroblasts and smooth muscle cells was observed. No inflammatory alterations, no granulomas and no multinucleated giant cells could be noted in the vascular walls and in the cerebral parenchyma. A small ischemic infarct was present in the left thalamus. Cerebellum, brainstem and medulla showed multiple areas of progressive multifocal leukoencephalopathy (PML). Immunohistochemistry with anti-gp41, a monoclonal antibody against HIV envelope did not exhibit any positive results. These findings implicate that the vascular lesion might be attributed to primary infection of the brain by HIV which led to a defect of elastic lamina and consecutive intimal hyperplasia. A second hypothesis could be based on the effect of extremely high dose AZT therapy avoiding inflammatory reaction after HIV infection.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"83-90"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12548060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunocytochemical characterization of cytomegalovirus (CMV) infected giant cells in perinatal acquired human immunodeficiency virus (HIV) infection. 围产期获得性人类免疫缺陷病毒(HIV)感染巨细胞病毒(CMV)的免疫细胞化学特征
Acta histochemica. Supplementband Pub Date : 1992-01-01
M Horn, W Schlote, G Herrmann, T Güngör, G Jacobi
{"title":"Immunocytochemical characterization of cytomegalovirus (CMV) infected giant cells in perinatal acquired human immunodeficiency virus (HIV) infection.","authors":"M Horn,&nbsp;W Schlote,&nbsp;G Herrmann,&nbsp;T Güngör,&nbsp;G Jacobi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In a pediatric case of necrotizing CMV myelitis after perinatal HIV infection characteristic cytomegalic cells, which could not be attached to a particular cell line by cell morphology, were studied after immunostaining with monoclonal and polyclonal antibodies raised against GFAP, S100 protein, NSE, synaptophysin, factor VIII, vimentin, macrophages, leukocytes, CMV, HSV I + II, toxoplasma, and HIV 1 gp41. Astrocytes, oligodendrocytes, neurons, ependymal and endothelial cells, macrophages, and Schwann cells stained positively with CMV antiserum. With regard to their immunological features the majority of cytomegalic cells (\"owl eye cells\") was identified as astrocytes, and in decreasing frequency, the remainder was characterized as macrophages, mesenchymal, and endothelial cells. It is concluded that CMV giant cells represent one phase of virus induced cell transformation, not only one single, but numerous cell types are exposed to after CMV infection.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"115-22"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12491033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transglutaminase activity in human brain tumors. 人类脑肿瘤中的谷氨酰胺转氨酶活性。
Acta histochemica. Supplementband Pub Date : 1992-01-01
G Röhn, R I Ernestus, R Schröder, K A Hossmann, N Klug, W Paschen
{"title":"Transglutaminase activity in human brain tumors.","authors":"G Röhn,&nbsp;R I Ernestus,&nbsp;R Schröder,&nbsp;K A Hossmann,&nbsp;N Klug,&nbsp;W Paschen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Transglutaminase (TG) activity was measured in tissue samples of 45 human brain tumors (pituitary adenomas, meningiomas and gliomas) obtained during neurosurgery. Biochemical analysis and histopathological classification were carried out in the same samples. Mean enzyme activity was highest in non-glial tumors, but due to a high variability of values no significant changes were found between the various histological groups. Thus, TG activity, although considered to play a role in neoplastic growth, does not represent a biochemical marker of malignancy in human brain tumors.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"155-8"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12523546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of astroglial cell proliferation in vitro and in vivo. 星形胶质细胞体外和体内增殖的表征。
Acta histochemica. Supplementband Pub Date : 1992-01-01
H Korr, E Siewert, U Strier, C Bertram, M Sensenbrenner
{"title":"Characterization of astroglial cell proliferation in vitro and in vivo.","authors":"H Korr,&nbsp;E Siewert,&nbsp;U Strier,&nbsp;C Bertram,&nbsp;M Sensenbrenner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>On the basis of experimental set-ups in vitro and in vivo and by making use of specific autoradiographical techniques, the following data on the proliferation of astrocytes from newborn rats in vitro and unpretreated rats and mice in vivo could be obtained: (i) The commonly employed immunohistochemical staining techniques in vitro are not applicable in tissue sections. (ii) In vivo, astrocytes show increasing durations of cell cycle (tc) as well as S phase (ts) prenatally until about birth. A similar trend can be observed in vitro. However, the absolute values for ts and tc can be substantially modified depending on the culture conditions. (iii) As regards the mode of proliferation, astrocytes in vitro grow exponentially and without transition of quiescent cells from the non-growth fraction into the growth fraction (GF). In contrast, astrocytes in vivo exhibit steady-state growth and continuous recruitment of proliferating cells from the non-GF. These differences show that there is a need for further in vivo-experiments when studying new strategies in the treatment of gliomas.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"245-50"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12751938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[An intermediate, generalized form of neuroaxonal dystrophy--light- and electron microscopic findings]. [神经轴突营养不良的中间、一般形式——光镜和电镜发现]。
Acta histochemica. Supplementband Pub Date : 1992-01-01
J Lehmann, H H Goebel
{"title":"[An intermediate, generalized form of neuroaxonal dystrophy--light- and electron microscopic findings].","authors":"J Lehmann,&nbsp;H H Goebel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This report concerns a 11-year-old girl--at the time of death--who developed normally until the age of 2 years when further psychomotoric maturation stopped and then regressed. The disease was diagnosed as neuroaxonal dystrophy (NAD) by sural nerve biopsy at the age of 7. Further course was characterized by complete loss of all motoric and sensory functions and dementia as well. Finally there was decerebration. The autopsy revealed generalized NAD associated with pallidal deposition of iron pigment to classify as generalized intermediate NAD type II according to Gilman and Barrett (1973). The main histological findings were axonal swellings and spheroids consisting ultrastructurally of membrano-tubular profiles, lamellar structures, vacuoles, glycogen granules and mitochondrial aggregates. Immunohistologically there was partial positive expression of synaptophysin and neurofilament protein in the spheroids. Firstly described electron microscopical findings in the retina include the typical axonal lesions largely in interior layers. Photoreceptors and their synaptic contacts were preserved. The present blindness is of the neuronal type. The current etiopathogenetic opinions, aspects of bioptic diagnosis and problems of classification of primary NADs are discussed.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"311-8"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12752636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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