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Combined erbB gene overexpression and decreased H-ras gene expression in human gliomas. 脑胶质瘤中erbB基因过表达和H-ras基因表达降低的联合研究。
Acta histochemica. Supplementband Pub Date : 1992-01-01
S Patt, J Cervós-Navarro
{"title":"Combined erbB gene overexpression and decreased H-ras gene expression in human gliomas.","authors":"S Patt,&nbsp;J Cervós-Navarro","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The expression of 15 oncogenes including erbB and H-ras in 18 human glial tumors -10 glioblastomas, 1 astrocytoma grade III-IV, 2 oligodendrogliomas grade III, 2 astrocytomas grade II-III, 1 astrocytoma grade II, 1 oligodendroglioma grade II and 1 oligoastrocytoma grade II--was determined by hybridizing RNA against oncogene probes using the Dot Blot technique. Compared with bovine cerebrum (control), the oncogenes abl, erbA, fms, fos, K-ras, mil, mos, myb, rel, sis, src and yes were expressed equally in both bovine cerebrum and the gliomas. However, the expression of erbB was increased 2-9-fold in all except one glioma, and the expression of H-ras was decreased by the factor 0.3-0.7 in 15 tumors. No obvious correlation was found between tumor histology and changed expression of erbB and/or H-ras or between the grade of malignancy and the expression of any oncogene tested. A connection between erbB and H-ras has been shown by several studies. Our results confirm the relationship between H-ras and erbB. However, the meaning of the H-ras decrease in combination with the erbB elevation has to be clarified.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"131-8"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12752773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transglutaminase activity in human brain tumors. 人类脑肿瘤中的谷氨酰胺转氨酶活性。
Acta histochemica. Supplementband Pub Date : 1992-01-01
G Röhn, R I Ernestus, R Schröder, K A Hossmann, N Klug, W Paschen
{"title":"Transglutaminase activity in human brain tumors.","authors":"G Röhn,&nbsp;R I Ernestus,&nbsp;R Schröder,&nbsp;K A Hossmann,&nbsp;N Klug,&nbsp;W Paschen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Transglutaminase (TG) activity was measured in tissue samples of 45 human brain tumors (pituitary adenomas, meningiomas and gliomas) obtained during neurosurgery. Biochemical analysis and histopathological classification were carried out in the same samples. Mean enzyme activity was highest in non-glial tumors, but due to a high variability of values no significant changes were found between the various histological groups. Thus, TG activity, although considered to play a role in neoplastic growth, does not represent a biochemical marker of malignancy in human brain tumors.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"155-8"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12523546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of astroglial cell proliferation in vitro and in vivo. 星形胶质细胞体外和体内增殖的表征。
Acta histochemica. Supplementband Pub Date : 1992-01-01
H Korr, E Siewert, U Strier, C Bertram, M Sensenbrenner
{"title":"Characterization of astroglial cell proliferation in vitro and in vivo.","authors":"H Korr,&nbsp;E Siewert,&nbsp;U Strier,&nbsp;C Bertram,&nbsp;M Sensenbrenner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>On the basis of experimental set-ups in vitro and in vivo and by making use of specific autoradiographical techniques, the following data on the proliferation of astrocytes from newborn rats in vitro and unpretreated rats and mice in vivo could be obtained: (i) The commonly employed immunohistochemical staining techniques in vitro are not applicable in tissue sections. (ii) In vivo, astrocytes show increasing durations of cell cycle (tc) as well as S phase (ts) prenatally until about birth. A similar trend can be observed in vitro. However, the absolute values for ts and tc can be substantially modified depending on the culture conditions. (iii) As regards the mode of proliferation, astrocytes in vitro grow exponentially and without transition of quiescent cells from the non-growth fraction into the growth fraction (GF). In contrast, astrocytes in vivo exhibit steady-state growth and continuous recruitment of proliferating cells from the non-GF. These differences show that there is a need for further in vivo-experiments when studying new strategies in the treatment of gliomas.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"245-50"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12751938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Qualitative studies of Fahr disease]. [Fahr病的定性研究]。
Acta histochemica. Supplementband Pub Date : 1992-01-01
J Schmitt, K Dietzmann, P von Bossányi
{"title":"[Qualitative studies of Fahr disease].","authors":"J Schmitt,&nbsp;K Dietzmann,&nbsp;P von Bossányi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We investigated brain tissue from 7 deceased patients with Fahr's disease using X-ray-analysis for the determination of compositional differences between calcareous particles in different areas of brain or between the left and right sides in a brain and differences in composition of concrements in different brains. We found, in all areas of one brain no difference in elementary composition. Differences were found between different brains, two brains showed a high silicon content.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"319-24"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12752637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Current aspects in diagnosis of Parkinson disease]. 【帕金森病的诊断现状】。
Acta histochemica. Supplementband Pub Date : 1992-01-01
R van der Valk, J Jörg
{"title":"[Current aspects in diagnosis of Parkinson disease].","authors":"R van der Valk,&nbsp;J Jörg","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The causes of symptomatic parkinsonism are enumerated and discussed including drug-induced, vascular, toxic, postencephalitic and posttraumatic parkinsonism. The environmental hypothesis and the concept of oxidative stress in the pathogenesis of Parkinson's disease are illustrated. The clinical diagnosis, the differential diagnosis and the possible diagnostic errors originating from the cardinal symptoms akinesia, rigor und tremor in the early stages of the disease are delineated. At last the contributions of EEG, CCT, evoked potentials, MRI, PET und the apomorphine test to the diagnosis especially early diagnosis are evaluated.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"51-8"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12751715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The distribution of intermediate filaments in intracranial vessels in humans]. [人类颅内血管中中间纤维的分布]。
Acta histochemica. Supplementband Pub Date : 1992-01-01
W Roggendorf, B Künzig
{"title":"[The distribution of intermediate filaments in intracranial vessels in humans].","authors":"W Roggendorf,&nbsp;B Künzig","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The distribution of intermediate filaments GFAP, Vimentin and Desmin was investigated in normal intracerebral and meningeal vessels in human material. Vimentin is detectable in endothelial cells of all cranial vessels. Moreover Vimentin is expressed in all vascular smooth muscle cells of the brain with the exception of arterioles in the white matter and in the pons. Desmin is only detectable in the tunica media of larger intracerebral arterioles or arteries, larger meningeal veins contain single Desmin positive smooth muscle cells. Immuno-electronmicroscopically Vimentin could be shown in endothelial and smooth muscle cells of larger venous and arteriolar vessels. We conclude from our immunohistologic investigation that there is an uneven distribution of Vimentin and Desmin in brain vessels which might reflect some ultrastructural peculiarities of brain arteries.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"99-106"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12752418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunohistochemical reactivity of neuropeptides in plastic-embedded semithin sections of the myenteric plexus in infantile hypertrophic pylorus stenosis. 婴儿肥厚性幽门狭窄肌肠丛塑料包埋半薄切片中神经肽的免疫组化反应性。
Acta histochemica. Supplementband Pub Date : 1992-01-01
J M Schröder, R Dieler, H Skopnik, G Steinau
{"title":"Immunohistochemical reactivity of neuropeptides in plastic-embedded semithin sections of the myenteric plexus in infantile hypertrophic pylorus stenosis.","authors":"J M Schröder,&nbsp;R Dieler,&nbsp;H Skopnik,&nbsp;G Steinau","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pyloric biopsies obtained at pyloromyotomy from 46 infants were studied by light and electron microscopy and compared to 8 autopsy control cases without any evidence of infantile hypertrophic pyloric stenosis (IHPS). A positive family history of this disorder was recorded in 8 cases (2 girls and 6 boys). The most frequent changes in the myenteric plexus comprised axonal alterations. In glial cells, cytoplasmic vacuolisation or an increase of intermediate filaments occurred. In ganglion cells, vacuolisation of perikaryal cytoplasm or dense bodies were observed. No obvious differences were seen between sporadic and hereditary cases. In addition to these fine structural alterations, immunohistochemistry in 6 IHPS cases and 4 controls revealed differences in the distribution of substance P, bombesin, calcitonin gene related peptide and enkephalin-like immunoreactivity within the myenteric plexus between IHPS and control cases. The immunoreactivity, however, was unevenly distributed from case to case and even within individual cases. The reduction of immunoreactivity corresponded, at least in part, to an increase of neurofilaments or abnormal organelles within axons. Increased immunoreactivity was apparently related to focal accumulation of dense cored vesicles noted in the preceding study. It is suggested that these and other changes reported interfere with the normal gastrointestinal reflex mechanisms leading to intestinal obstruction.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"341-4"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12751713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of the AgNOR technique to neurooncology. AgNOR技术在神经肿瘤学中的应用。
Acta histochemica. Supplementband Pub Date : 1992-01-01
K H Plate, J Rüschoff, H D Mennel
{"title":"Application of the AgNOR technique to neurooncology.","authors":"K H Plate,&nbsp;J Rüschoff,&nbsp;H D Mennel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The silver staining of interphase nucleolar organizer regions (NORs) is a recently developed method to measure cell proliferation in tissue specimens. The major silver staining protein is nucleolin, a 92 kd nucleolar protein, which probably controls rDNA transcription. Nucleolin itself is under control of p34cdc2 kinase, which is a subunit of M phase kinase. The specific silver staining of NORs measures ribosomal gene activity and is therefore useful in measuring cell proliferation via nucleolar biosynthetic activity. Although human tumors of different malignancy grades usually can be distinguished by their AgNOR number, there is considerable overlap between different grades which significantly hampers their use in individual cases. For routine application of the AgNOR technique in histopathology we propose a standardised staining protocol with use of internal control cells and the use of image analysis for AgNOR enumeration.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"171-8"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12751928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[AgNOR in neurooncology]. [神经肿瘤学中的AgNOR]。
Acta histochemica. Supplementband Pub Date : 1992-01-01
H Martin, J Gottschalk, P Hufnagl, M Beil
{"title":"[AgNOR in neurooncology].","authors":"H Martin,&nbsp;J Gottschalk,&nbsp;P Hufnagl,&nbsp;M Beil","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>By means of a standardized silver staining technique, nucleolar organizer region-associated proteins (AgNORs) have been demonstrated in paraffin sections of 41 astrocytomas and glioblastomas and 19 meningiomas. Computer-assisted image analysis was used to measure AgNORs together with other nuclear features. Significant differences in number and configuration of AgNORs have been found between non-anaplastic astrocytomas and anaplastic gliomata. In meningiomas, tumours with signs of increased proliferative activity and usual non-anaplastic ones could be discriminated using AgNOR data.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"179-84"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12751929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lysosomal proteinases as putative diagnostic tools in human neuropathology: Alzheimer disease (AD) and schizophrenia. 溶酶体蛋白酶作为假定的诊断工具在人类神经病理学:阿尔茨海默病(AD)和精神分裂症。
Acta histochemica. Supplementband Pub Date : 1992-01-01
H G Bernstein, H Kirschke, B Wiederanders, R M Khudoerkov, W Hinz, A Rinne
{"title":"Lysosomal proteinases as putative diagnostic tools in human neuropathology: Alzheimer disease (AD) and schizophrenia.","authors":"H G Bernstein,&nbsp;H Kirschke,&nbsp;B Wiederanders,&nbsp;R M Khudoerkov,&nbsp;W Hinz,&nbsp;A Rinne","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The cathepsin B, D and L were studied by immunohistochemical techniques in the human postmortem brain. The enzyme were primarily localized in neurons. Makroglial cells were seldom immunostained. It is shown that cathepsins B and D frequently occur in neuritic plaques of Alzheimer victims, thereby raising the question, whether or not cathepsin immunohistochemistry is a useful tool in the diagnosis of this disease. Furthermore, we identified certain glial cells to be immunoreactive for cathepsins in schizophrenics.</p>","PeriodicalId":7002,"journal":{"name":"Acta histochemica. Supplementband","volume":"42 ","pages":"19-24"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12751931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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