用催化酶组织化学的方法来研究神经系统是否还足够?

Acta histochemica. Supplementband Pub Date : 1992-01-01
R Gossrau, W Richter
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引用次数: 0

摘要

不同于酶组织化学方法的频繁使用引发了一个问题,催化酶组织化学方法在神经系统研究中是否仍然有用?在这个简短的概述显示,催化酶组织化学仍然可以有助于更好地理解神经系统的功能。这得益于方法学的进步,即对现有程序的修改或利用组织切片中酶的催化特性进行可视化和测量的新技术的开发。举例说明了乙酰胆碱酯酶、单胺氧化酶以及某些外糖苷酶和磷酸酶的测定方法。这些方法的应用以及对蛋白酶、产生氧化自由基的氧化酶和参与氨基酸和其他递质的代谢的酶的方法将说明,定性(定位)和定量(测量)催化酶组织化学仍然有助于更好地理解神经系统的功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Is it still adequate to study the nervous system using methods of catalytic enzyme histochemistry?

The frequent use of methods different from enzyme histochemistry evokes the question, whether it is still useful to apply methods of catalytic enzyme histochemistry to study the nervous system. In this brief overview it is shown, that catalytic enzyme histochemistry can still contribute to a better understanding of nervous system function. This was enabled by methodological progress, i.e., the modification of already existing procedures or the development of new techniques for the visualization and measurement of enzymes in tissue sections using their catalytic properties. The methods for acetylcholinesterase, monoamine oxidase as well as certain exoglycosidases and phosphatases will be given as examples. The application of these procedures as well as of methods for proteases, oxyradical-generating oxidases and enzymes involved in the metabolism of amino acid and other transmitters will illustrate, that qualitative (localization) and quantitative (measurement) catalytic enzyme histochemistry can still contribute to a better understanding of nervous system function.

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