Acta Cytologica最新文献

筛选
英文 中文
Overcoming Pitfalls in Breast Fine-Needle Aspiration Cytology: A Practical Review. 克服乳腺 FNAC 的误区:实用综述。
IF 1.6 4区 医学
Acta Cytologica Pub Date : 2024-01-01 Epub Date: 2024-06-11 DOI: 10.1159/000539692
Daniel Gomes Pinto, Fernando C Schmitt
{"title":"Overcoming Pitfalls in Breast Fine-Needle Aspiration Cytology: A Practical Review.","authors":"Daniel Gomes Pinto, Fernando C Schmitt","doi":"10.1159/000539692","DOIUrl":"10.1159/000539692","url":null,"abstract":"<p><strong>Background: </strong>Fine-needle aspiration cytology (FNAC) is a cornerstone technique for the initial assessment of breast lesions, offering a rapid and minimally invasive option for cytological evaluation. While FNACs can forego the need for core needle biopsies (CNBs), variations in technique, subjective interpretation, and intrinsic limitations present diagnostic challenges. The International Academy of Cytology (IAC) established the Yokohama system and is developing the WHO Reporting System for Breast Cytopathology jointly with IARC, to standardize diagnostic criteria, aiming to enhance diagnostic precision and consistency. Due to the preference for CNBs, expertise in breast FNAC is low in the developed world.</p><p><strong>Summary: </strong>This review assesses common pitfalls in breast cytopathology. These common and uncommon entities may easily lead to false-negative or false-positive diagnoses, due to morphological overlap or misleading clinical and radiological contexts. For instance, pauci-cellular lesions, such as lobular carcinomas, often lead to false-negative diagnoses, whereas complex sclerosing lesions, fibroadenomas, and papillary lesions may show concerning features, resulting in a false positive. The same is true for some benign inflammatory pathologies, such as steatonecrosis, and uncommon lesions, such as collagenous spherulosis. Ductal carcinoma in situ can lead to both false-negative and false-positive diagnoses, and high-grade lesions are impossible to tell apart from invasive carcinomas. These are discussed in detail. Procedural and preanalytical conditions, and the role of ancillary testing, are also briefly addressed.</p><p><strong>Key messages: </strong>Breast FNAB is a powerful diagnostic technique, fast and minimally invasive. Even in contexts which lack expertise, this technique can be successfully adopted with a cautious approach and as long as pitfalls are kept in mind, benefiting patients and healthcare systems.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"206-218"},"PeriodicalIF":1.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficacy of Fine-Needle Aspiration Cytology in Diagnosing Secretory Carcinoma of Salivary Gland: A Systematic Review and Meta-Analysis. 细针穿刺细胞学诊断唾液腺分泌性癌的有效性:系统回顾与荟萃分析。
IF 1.6 4区 医学
Acta Cytologica Pub Date : 2024-01-01 Epub Date: 2024-01-16 DOI: 10.1159/000536249
Pooja Sharma Kala, Mamta Gupta, Naveen Thapliyal
{"title":"Efficacy of Fine-Needle Aspiration Cytology in Diagnosing Secretory Carcinoma of Salivary Gland: A Systematic Review and Meta-Analysis.","authors":"Pooja Sharma Kala, Mamta Gupta, Naveen Thapliyal","doi":"10.1159/000536249","DOIUrl":"10.1159/000536249","url":null,"abstract":"<p><strong>Introduction: </strong>The diagnosis of salivary gland secretory carcinoma (SC) in fine-needle aspiration specimens is challenging because its low-grade nature makes it difficult to differentiate it from various benign or malignant salivary gland neoplasms. Currently, the gold standard is demonstration of ETV6-NTRK3 fusion gene. However, the decision for ordering this costly molecular testing can be facilitated by the correct recognition of its cytomorphological features. The aim of the review was to determine the accuracy of fine-needle aspiration cytology (FNAC) in diagnosis of salivary gland SC. The secondary objective was to recognize varied cytomorphological patterns, characteristic features of SC and differentiate it from other neoplasms.</p><p><strong>Methods: </strong>PubMed/MEDLINE, Science Direct, Embase, Cochrane review, and PROSPERO databases were searched for studies having the following key search terms: (\"secretory carcinoma of salivary gland\" OR \"mammary analogue secretory carcinoma of salivary gland\") AND (\"Cytology\" OR \"Cytological features\" OR \"aspirate\" OR \"cytodiagnosis\") published in the time frame of 2010 to June 2023. Studies reporting cytological features of the salivary gland tumors which were confirmed/diagnosed as SC on molecular investigation, were included in the systematic review. Finally, seventeen studies reporting a total of 45 cases were included in the metanalysis.</p><p><strong>Results: </strong>The sensitivity of the FNAC in diagnosing SC in salivary gland is 27.7% (95% CI: 16.6-42.5%). The LR+ (positive likelihood ratio) was 0.654 (0.344-1.245), LR- (negative likelihood ratio) was 1.023 (0.538-1.946), and diagnostic odds ratio was 0.421 (0.129-1.374). The molecular testing and/or immunohistochemistry performed on cell block increased the diagnostic accuracy.</p><p><strong>Conclusion: </strong>Recognition of subtle cytomorphological patterns, i.e., papillary formation, clusters, and singly dispersed cells along with presence of fine intracytoplasmic vacuolations were the characteristic findings in majority of cases, confirmed with diagnostic molecular profiling. This may be helpful in identification of this rare entity with limited published literature and help in increasing diagnostic accuracy.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"83-106"},"PeriodicalIF":1.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139477805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pitfalls in Salivary Gland Cytology. 唾液腺细胞学的陷阱。
IF 1.6 4区 医学
Acta Cytologica Pub Date : 2024-01-01 Epub Date: 2024-02-28 DOI: 10.1159/000538069
Carla Saoud, Gabrielle E Bailey, Ashleigh Graham, Lorena Marcano Bonilla, Sandra Ixchel Sanchez, Zahra Maleki
{"title":"Pitfalls in Salivary Gland Cytology.","authors":"Carla Saoud, Gabrielle E Bailey, Ashleigh Graham, Lorena Marcano Bonilla, Sandra Ixchel Sanchez, Zahra Maleki","doi":"10.1159/000538069","DOIUrl":"10.1159/000538069","url":null,"abstract":"<p><strong>Background: </strong>Salivary gland lesions possess diagnostic challenges on fine-needle aspiration (FNA) material. They are relatively uncommon, yet present with a wide spectrum of cytomorphology. Herein, we review common salivary gland neoplasms, their cytomorphologic features, their diagnostic pitfalls, and ancillary studies helpful in achieving an accurate diagnosis.</p><p><strong>Summary: </strong>There are many cytomorphologic overlaps between benign and malignant salivary gland entities. Moreover, metaplasia, cystic changes, and degenerative changes are common findings adding to diagnostic dilemmas. These complicating factors contribute to a minute risk of malignancy in salivary gland lesions that are interpreted as benign on FNA. In rare cases, even malignant salivary gland neoplasms are misinterpreted as benign on aspirated material due to the many cytomorphologic overlaps. For example, benign and malignant neoplasms containing stroma such as myoepithelioma and adenoid cystic carcinoma may be misinterpreted as pleomorphic adenoma. Moreover, diagnosis of salivary gland neoplasms with basal cell features can be confusing on FNA materials; for example, basal cell adenoma can be misinterpreted as adenoid cystic carcinoma. Mucoepidermoid carcinomas have many different appearances on aspirated material due to variable amounts of mucin, degree of nuclear atypia, cellular content, and squamous metaplasia. Acinic cell carcinoma exhibits large cells with abundant cytoplasm on FNA, which can be mistaken for oncocytic cells in oncocytoma or Warthin tumor. Salivary duct carcinoma shows distinct features of malignancy and thus can be mistaken for secondary tumors involving the salivary glands or other malignant salivary gland tumors. The presence of tumor-associated lymphocytes is another underlying cause of misdiagnosis, especially when considering the differential diagnosis of an intraparotid lymph node. Ancillary studies such as immunohistochemistry and molecular studies are gaining more attention to be utilized on FNA cases. PLAG1 immunostaining, CD117, DOG1, mammaglobin, and androgen receptor (AR) are examples of commonly used immunostains in diagnosis of salivary gland lesions. MYB gene fusion, rearrangements of the MAML2 gene, and ERBB2/HER2 are examples of molecular alterations useful in diagnosis of salivary gland neoplasms. In conclusion, the aim of salivary gland cytology is to differentiate benign entities from the malignant ones and to prevent unnecessary aggressive treatments.</p><p><strong>Key messages: </strong>The diagnostic pitfalls are enormous in salivary gland cytology. Familiarity with cytomorphology of different entities and their cytomorphologic overlaps, and application of ancillary studies improves the diagnostic yield, patient management and prevents unnecessary aggressive procedures.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"194-205"},"PeriodicalIF":1.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reproducibility of the Paris System in Urine Cytology by Third-Year Pathology Residents. 病理科三年级住院医师尿液细胞学中巴黎系统的再现性。
IF 1.6 4区 医学
Acta Cytologica Pub Date : 2024-01-01 Epub Date: 2024-07-24 DOI: 10.1159/000540533
Gamze Kavas, Asena Demiröz, Sinem Eser Polat Unal, Ahmet Cahit Calışkan, Gözde Ilhan, Betül Celik
{"title":"Reproducibility of the Paris System in Urine Cytology by Third-Year Pathology Residents.","authors":"Gamze Kavas, Asena Demiröz, Sinem Eser Polat Unal, Ahmet Cahit Calışkan, Gözde Ilhan, Betül Celik","doi":"10.1159/000540533","DOIUrl":"10.1159/000540533","url":null,"abstract":"<p><strong>Introduction: </strong>Standardized basic morphology and the algorithmic approach make the Paris System (TPS) for Reporting Urinary Cytology understandable and applicable. This study examined how well the TPS categories are understood by pathology residents and how well these criteria are enabling them reaching accurate diagnosis.</p><p><strong>Materials/methods: </strong>A hundred consecutive cases representing all categories were selected. Authors reevaluated slides using TPS regardless of their original diagnosis. In the next step, the TPS was explained to four residents and trained them by five optimal urine cytology samples from each category. Then they were asked to diagnose the selected slides according to the TPS. The diagnoses were compared to authors. The agreement was assessed using kappa. Discordant diagnoses were classified as high and low impact based on potential on clinical practice.</p><p><strong>Results: </strong>The sensitivity of authors was 62.8%, and residents' were 24-31.8%. The specificity of authors was 98.8%, and residents' were 82.3-92.8%. Reproducibility of TPS was 40-46%. Kappa values were below 0.40 except for one resident. The highest rate of concordance was for negative for high-grade urothelial carcinoma (NHGUC): authors assigned 38 NHGUC (35 biopsy-proven benign cases). Twenty to twenty-six of them were assigned as NHGUC by residents. While authors assigned 42 cases as suspicious for high-grade urothelial carcinoma (SHGUC) or high-grade urothelial carcinoma (HGUC) (35 biopsy-proven malignant cases), residents assigned 22-29 of them. Discordant diagnosis with high clinical implication was 56-63%.</p><p><strong>Conclusion: </strong>Diagnostic accuracy rates of junior pathology residents using the TPS were unsatisfactory. The best agreement was observed in NHGUC and HGUC categories. Combining HGUC and SHGUC doubled the sensitivity of residents.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"465-471"},"PeriodicalIF":1.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diagnostic Accuracy of Papanicolaou Society of Cytopathology System for Reporting Respiratory Cytology: A Systematic Review and Meta-Analysis. 帕氏细胞病理学协会系统报告呼吸道细胞学的诊断准确性:系统回顾与元分析》。
IF 1.6 4区 医学
Acta Cytologica Pub Date : 2024-01-01 Epub Date: 2024-08-27 DOI: 10.1159/000541139
Sana Ahuja, Marzieh Fattahi-Darghlou, Sufian Zaheer, Rhea Ahuja
{"title":"Diagnostic Accuracy of Papanicolaou Society of Cytopathology System for Reporting Respiratory Cytology: A Systematic Review and Meta-Analysis.","authors":"Sana Ahuja, Marzieh Fattahi-Darghlou, Sufian Zaheer, Rhea Ahuja","doi":"10.1159/000541139","DOIUrl":"10.1159/000541139","url":null,"abstract":"<p><strong>Introduction: </strong>This study conducts the first meta-analysis to evaluate the diagnostic accuracy and the aggregated risk of malignancy associated with each category of the Papanicolaou Society of Cytopathology (PSC) system for reporting respiratory cytology.</p><p><strong>Methods: </strong>A systematic search was conducted in PubMed, Scopus, and Web of Science using the keywords \"(Lung, Respiratory specimens) AND (Papanicolaou Society of Cytopathology System).\" Articles were assessed for risk of bias using the QUADAS-2 tool. After excluding inadequate samples, sensitivity and specificity for various cut-off points. Summary receiver operating characteristic curves and diagnostic odds ratios were pooled to assess diagnostic accuracy.</p><p><strong>Results: </strong>Five studies, totaling 3,489 cases, were included. Sensitivity and specificity for the \"Atypical and higher risk categories\" considered positive were 60% (95% CI, 51-68%) and 87% (95% CI, 81-92%), respectively. For the \"Suspicious for malignancy and higher risk categories\" considered positive, sensitivity and specificity were 49% (95% CI, 40-58%) and 95% (95% CI, 92-97%), respectively. Sensitivity and specificity for the \"Malignant\" category considered positive for malignancy were 42% (95% CI, 33-52%) and 97% (95% CI, 92-99%), respectively. The pooled area under the curve ranged from 68 to 75% for each cut-off.</p><p><strong>Conclusion: </strong>This meta-analysis underscores the PSC system's accuracy in reporting respiratory cytology. It highlights the diagnostic importance of the \"Suspicious\" and \"Malignant\" categories in identifying malignancy, and the utility of the \"Atypical\" category for initial screening. These findings support the PSC system's role in enhancing diagnostic accuracy and clinical decision-making in respiratory cytology.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"450-464"},"PeriodicalIF":1.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142078780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Message from the International Academy of Cytology. 来自国际细胞学学会的信息。
IF 1.6 4区 医学
Acta Cytologica Pub Date : 2024-01-01 Epub Date: 2024-10-25 DOI: 10.1159/000542042
{"title":"Message from the International Academy of Cytology.","authors":"","doi":"10.1159/000542042","DOIUrl":"10.1159/000542042","url":null,"abstract":"","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":"68 5","pages":"494"},"PeriodicalIF":1.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Novel Validated Real-World Dataset for the Diagnosis of Multiclass Serous Effusion Cytology according to the International System and Ground-Truth Validation Data. 根据 TIS 和地面实况验证数据诊断多类浆液性渗出细胞学的新型验证真实世界数据集。
IF 1.6 4区 医学
Acta Cytologica Pub Date : 2024-01-01 Epub Date: 2024-03-24 DOI: 10.1159/000538465
Esraa Abd-Almoniem, Nadia Abd-Alsabour, Samar Elsheikh, Rasha R Mostafa, Yasmine Fathy Elesawy
{"title":"A Novel Validated Real-World Dataset for the Diagnosis of Multiclass Serous Effusion Cytology according to the International System and Ground-Truth Validation Data.","authors":"Esraa Abd-Almoniem, Nadia Abd-Alsabour, Samar Elsheikh, Rasha R Mostafa, Yasmine Fathy Elesawy","doi":"10.1159/000538465","DOIUrl":"10.1159/000538465","url":null,"abstract":"<p><strong>Introduction: </strong>The application of artificial intelligence (AI) algorithms in serous fluid cytology is lacking due to the deficiency in standardized publicly available datasets. Here, we develop a novel public serous effusion cytology dataset. Furthermore, we apply AI algorithms on it to test its diagnostic utility and safety in clinical practice.</p><p><strong>Methods: </strong>The work is divided into three phases. Phase 1 entails building the dataset based on the multitiered evidence-based classification system proposed by the International System (TIS) of serous fluid cytology along with ground-truth tissue diagnosis for malignancy. To ensure reliable results of future AI research on this dataset, we carefully consider all the steps of the preparation and staining from a real-world cytopathology perspective. In phase 2, we pay special consideration to the image acquisition pipeline to ensure image integrity. Then we utilize the power of transfer learning using the convolutional layers of the VGG16 deep learning model for feature extraction. Finally, in phase 3, we apply the random forest classifier on the constructed dataset.</p><p><strong>Results: </strong>The dataset comprises 3,731 images distributed among the four TIS diagnostic categories. The model achieves 74% accuracy in this multiclass classification problem. Using a one-versus-all classifier, the fallout rate for images that are misclassified as negative for malignancy despite being a higher risk diagnosis is 0.13. Most of these misclassified images (77%) belong to the atypia of undetermined significance category in concordance with real-life statistics.</p><p><strong>Conclusion: </strong>This is the first and largest publicly available serous fluid cytology dataset based on a standardized diagnostic system. It is also the first dataset to include various types of effusions and pericardial fluid specimens. In addition, it is the first dataset to include the diagnostically challenging atypical categories. AI algorithms applied on this novel dataset show reliable results that can be incorporated into actual clinical practice with minimal risk of missing a diagnosis of malignancy. This work provides a foundation for researchers to develop and test further AI algorithms for the diagnosis of serous effusions.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"160-170"},"PeriodicalIF":1.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140206160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of Pap Smear Cervical Cytology in the Diagnosis of Extrauterine Malignancies: Largest Study of 104 Cases from Tertiary Care Cancer Centre in India. 巴氏涂片宫颈细胞学在宫外恶性肿瘤诊断中的作用:印度三级癌症中心 104 例病例的最大规模研究。
IF 1.6 4区 医学
Acta Cytologica Pub Date : 2024-01-01 Epub Date: 2024-06-28 DOI: 10.1159/000539508
Arshi Tandon, Kanwalpreet Kaur, Majal Shah, Priti Trivedi
{"title":"Role of Pap Smear Cervical Cytology in the Diagnosis of Extrauterine Malignancies: Largest Study of 104 Cases from Tertiary Care Cancer Centre in India.","authors":"Arshi Tandon, Kanwalpreet Kaur, Majal Shah, Priti Trivedi","doi":"10.1159/000539508","DOIUrl":"10.1159/000539508","url":null,"abstract":"<p><strong>Introduction: </strong>Extrauterine malignancies in cervical samples are rarely seen. It is important to differentiate these cells from those of primary uterine malignancies to determine appropriate line of further investigations and management. Literature on these lesions is limited largely restricted to case reports. The aim of the present study was to study the spectrum and cytomorphological features of extrauterine malignancies in cervical Pap smears.</p><p><strong>Materials and methods: </strong>It is a retrospective and descriptive study conducted in Department of Cytopathology from January 2019 to July 2023. All cases of extrauterine malignancies with available cytology material were included in this study. All cases of primary uterine malignancies, i.e., uterine corpus or cervix confirmed by clinical, radiological, and histopathological examination were excluded.</p><p><strong>Results: </strong>104 out of 11,674 cytology Pap smears were those of extrauterine malignancy. Diagnosis of extrauterine malignancy was given in 47.1% cases, 30.9% were reported as positive for malignancy without giving the possibility of an extrauterine origin, and 22.0% were reported as atypical glandular cells only. In 56 cases where Pap smear was the first investigation which led to the diagnosis. Most common extrauterine malignancy was adenocarcinoma principally from ovarian, colorectal, and vaginal origin. Other epithelial malignancies noted were urothelial carcinoma and invasive breast carcinoma. Among non-epithelial malignancies, we reported vaginal mucosal melanoma, cutaneous melanoma, acute leukaemia, and anaplastic large cell lymphoma.</p><p><strong>Conclusion: </strong>High index of suspicion, presence of squamous and glandular elements with no atypical features, and occasional clusters of cells with marked atypia and usually no necrosis in the background are helpful cytomorphological clues to raise suspicion for extrauterine malignancy. Correlation with serology, radiology, and immunocytochemistry can help in reaching final diagnosis.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"319-326"},"PeriodicalIF":1.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141475718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Tumor Fraction Estimated by Rapid On-Site Evaluation Is Useful for Assessing the Suitability of Biopsy Specimens for Multiplex Genetic Testing. 通过现场快速评估估算出的肿瘤比例可用于评估活检样本是否适合进行多重基因检测。
IF 1.6 4区 医学
Acta Cytologica Pub Date : 2024-01-01 Epub Date: 2024-07-24 DOI: 10.1159/000540451
Toshitaka Uehara, Daisuke Kawashima, Naoya Kanatani, Erika Hiraguchi, Kento Yoshida, Yosuke Masumoto, Yusuke Kuboyama, Yuka Hiraki, Emi Inumaru, Yoshihiro Ohishi
{"title":"The Tumor Fraction Estimated by Rapid On-Site Evaluation Is Useful for Assessing the Suitability of Biopsy Specimens for Multiplex Genetic Testing.","authors":"Toshitaka Uehara, Daisuke Kawashima, Naoya Kanatani, Erika Hiraguchi, Kento Yoshida, Yosuke Masumoto, Yusuke Kuboyama, Yuka Hiraki, Emi Inumaru, Yoshihiro Ohishi","doi":"10.1159/000540451","DOIUrl":"10.1159/000540451","url":null,"abstract":"<p><strong>Introduction: </strong>Multiplex genetic testing (MGT) has become the mainstream method for genetic mutation testing in the field of lung cancer treatment, but the suitability criteria for MGT biopsy specimens are stringent. Although rapid on-site evaluation (ROSE) is considered a useful method for obtaining the suitable biopsy specimens for MGT, no direct comparisons of ROSE and MGT are available. In this study, we first evaluated the accuracy of MGT and ROSE in our hospital. Then, we explored the potential utility of the cytological findings of ROSE for indicating the adequacy of biopsy specimens for MGT.</p><p><strong>Methods: </strong>These analyses were performed retrospectively using the data of 74 patients with lung cancer who underwent ROSE at our hospital in 2020-2022.</p><p><strong>Results: </strong>Regarding the accuracy of MGT, the success rate was 97.9% and the frequency of epidermal growth factor receptor mutation in adenocarcinoma cases was 34.6%. The results of ROSE were then compared with histological diagnoses. The sensitivity and positive predictive value were 95.9% and 100.0%, respectively. To analyze the utility of the ROSE results for determining the adequacy of biopsy specimens for MGT, we determined the tumor fraction in the ROSE preparations (ROSE-T%) and the tumor fraction (B-T%)/tumor cell number (B-TN) in the biopsy specimens. When the threshold of the ROSE-T% was set at 80%, there were statistically significant biases of the B-T% ≥20%/B-TN ≥300 cases between the ROSE-T% ≥80% and <80% groups.</p><p><strong>Conclusion: </strong>This is the first report to suggest the utility of ROSE-T% in assessing the suitability of biopsy specimens for MGT. This predictive ability may add further value to ROSE and help reduce the time required for diagnostic testing, and thereby the patient burden.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"442-449"},"PeriodicalIF":1.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Does the Diagnostic Performance of the Pathologist on the Indeterminate Categories of the Bethesda System for Reporting Thyroid Cytopathology Vary between Pediatric and Adult Patients? 病理学家对贝塞斯达甲状腺细胞病理报告系统中不确定类别的诊断结果在儿童患者和成人患者之间是否存在差异?
IF 1.6 4区 医学
Acta Cytologica Pub Date : 2024-01-01 Epub Date: 2024-10-14 DOI: 10.1159/000541996
Sevgen Önder, Olcay Kurtulan, Cenk Sokmensuer, Gaye Guler, Diclehan Orhan
{"title":"Does the Diagnostic Performance of the Pathologist on the Indeterminate Categories of the Bethesda System for Reporting Thyroid Cytopathology Vary between Pediatric and Adult Patients?","authors":"Sevgen Önder, Olcay Kurtulan, Cenk Sokmensuer, Gaye Guler, Diclehan Orhan","doi":"10.1159/000541996","DOIUrl":"10.1159/000541996","url":null,"abstract":"<p><strong>Introduction: </strong>The objectives of this study were to investigate the variation in the use of indeterminate categories of The Bethesda System for reporting thyroid cytopathology across age groups, particularly focusing on atypia of undetermined significance (AUS) category, and discern the potential role of a bias in pathologist's interpretation when dealing with pediatric patients.</p><p><strong>Materials and methods: </strong>To highlight a more refined diagnostic pattern, thyroid fine-needle aspirations reported by a single pathologist over a span of 10 years enrolled to the study. A total of 8,827 cases from patients aged between 2 and 89 were categorized for each decades of ages. For the AUS category, AUS to malignant (AUS:M) ratio was calculated, and variations in distinct age groups and across the years were noted.</p><p><strong>Results: </strong>The rate of indeterminate categories was 19% for the pediatric cases and 11% for the adults. When compared to adults, AUS diagnosis was more common in pediatric patients (14% vs. 9%), with rates of malignancy (ROMs) 54% and 45%, respectively. The AUS:M ratio during the study period ranged between 0.5 and 2.3 (mean 1.4). AUS:M ratio was highest in children below the age 11. Among AUS subtypes, AUS-nuclear predominated in both age groups, and papillary thyroid carcinoma emerged as the most common malignancy after resection. Follicular neoplasia category was higher in pediatric patients than in adults (4% vs. 1%), with similar ROMs (29% vs. 32%).</p><p><strong>Conclusion: </strong>Indeterminate cytology diagnosis and subsequent ROM is higher in pediatric patients, which might be attributable to a slightly higher use of AUS diagnosis in the youngest children.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"506-515"},"PeriodicalIF":1.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信