Acta Cytologica最新文献

{"title":"Comparative Diagnostic Utility of Squash, Scrape and Imprint cytology in Intraoperative Diagnosis of Ovarian Tumours.","authors":"Gajendra Kumar Yadav, Ravi H Phulware, Ashok Singh, Arvind Kumar, Prashant Durgapal, Nilotpal Chowdhury, Shalinee Rao, Shalini Rajaram, Sanjeev Kishore, Rajlaxmi Mundhara","doi":"10.1159/000545110","DOIUrl":"https://doi.org/10.1159/000545110","url":null,"abstract":"<p><strong>Introduction: </strong>Intraoperative cytology in ovarian tumors involves collecting cell samples from the ovarian sample sent during surgery and quickly examining them for diagnostic information. Frozen section provides rapid diagnosis to guide intraoperative patient management. The indications of frozen section are identification of tissue, evaluation of margins and identification of lymph nodes metastasis.</p><p><strong>Aim: </strong>To evaluate comparative diagnostic utility of squash smear, scrape smear and imprint cytology with frozen section in intraoperative ovarian tumour.</p><p><strong>Materials and methods: </strong>Intraoperative tissue from clinico-radiologically suspected ovarian. tumour for frozen section taken and processed in Department of Pathology and Laboratory Medicine. Squash smear, scrape smear and imprint smear were made. Three stains (Rapid MGG, Rapid Pap and Rapid H &amp; E) with expected TAT of <15 minutes were done. Intraoperative cytological smear (squash, scrape and imprint smear) were correlated with frozen section &amp; Histopathology slide. Final assessment of intraoperative cytological smears for diagnostic accuracy was done using statistical study.</p><p><strong>Results: </strong>Sensitivity, Specificity, and Diagnostic accuracy for frozen and cytology were: Sensitivity of frozen section, squash cytology and scrape cytology was 91.67% in all three, whereas sensitivity of imprint was 87.5%. Specificity of frozen section, imprint cytology, squash cytology and scrape cytology was 96.77%, 93.55%, 90.32% and 90.32% respectively and accuracy was 94.55%, 90.91%, 90.91% and 90.91% respectively.</p><p><strong>Conclusion: </strong>Imprint, squash, and scrape cytology has similar sensitivity and specificity compared to frozen section in identifying the nature of lesion and can be an alternative to frozen section in resource stricken setting.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-19"},"PeriodicalIF":1.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Nucleic Acid Quality in Unstained Cytology Specimens for Cancer Genomic Testing.","authors":"Hiroya Niimi, Takafumi Onishi, Shoma Nomura, Aya Kumazaki, Yuto Masaki, Hirokazu Odashima, Yukihiko Osawa, Manabu Hattori","doi":"10.1159/000545423","DOIUrl":"https://doi.org/10.1159/000545423","url":null,"abstract":"<p><strong>Introduction: </strong>The use of cytological specimens in cancer genome medicine has garnered considerable attention, but the long-term quality of nucleic acids from unstained specimens remains unclear. This study aimed to evaluate the quality of nucleic acids extracted from unstained specimens fixed with 95% ethanol or spray fixation over varying durations.</p><p><strong>Methods: </strong>Two lung cancer cell lines were prepared using the auto smear method and fixed with 95% ethanol, and spray-fixed specimens were stored for 30 min, 1 day, 3 days, 1 week, 2 weeks, 1 month, 3 months, and 6 months. DNA was extracted using a DNA extraction kit, and quality was assessed using agarose gel electrophoresis and PCR.</p><p><strong>Results: </strong>Nucleic acids extracted from unstained specimens showed no fragmentation after six months of fixation and were amplifiable by PCR, regardless of the fixation method.</p><p><strong>Conclusion: </strong>Nucleic acids extracted from unstained specimens preserved high quality over six months, suggesting that such specimens are suitable for genetic testing. This finding has significant implications for the long-term storage and clinical application of cytological specimens in cancer genome medicine.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-12"},"PeriodicalIF":1.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Warthin's tumour diagnostic outcome in the Milan System era and cytomorphological pitfalls.","authors":"Henri Lagerstam, David Kalfert, Ivana Kholová","doi":"10.1159/000544973","DOIUrl":"https://doi.org/10.1159/000544973","url":null,"abstract":"<p><strong>Background: </strong>Warthin´s tumor (WT) is the second most common salivary gland neoplasm. With classic cytomorphological features of WT, the diagnostic accuracy is over 95%. WT is usually categorized as benign neoplasm according to the Milan System for Reporting Salivary Gland Cytopathology (MSRSGC).</p><p><strong>Methods: </strong>Database search at the Department of Pathology, Fimlab Laboratories, Tampere, Finland revealed 146 WTs during a 10-year period (January 1st, 2013-December 31st, 2022). Diagnostic accuracy was calculated for the entire study period, and the study period divided in half to pre-MSRSGC years (2013-2017) and MSRSGC years (2018-2022). In addition, a separate cytomorphology analysis of false-negative cases that were classified according to the MSRSGC, was performed.</p><p><strong>Results: </strong>Diagnostic accuracy was 96.4%, sensitivity was 68.5%, and specificity was 99.8%. Sensitivities and specificities were almost equal during the pre-MSRSGC years and the MSRSGC years. The number of true-positive cases was 113. Fifty-five cases (52 false-negative and three false-positive cases) were not accurately diagnosed. Risk of malignancy and risk of neoplasm were 0.0% and 98.3% of cases that were cytologically diagnosed as WT. Cytomorphological analysis showed that lack of papillae, the presence of small groups, and cystic degeneration led to false diagnoses. In addition, necrosis and diffuse hypercellularity increased the suspicion of malignancy and led to classification of fine-needle aspirations as salivary gland neoplasm of uncertain malignant potential.</p><p><strong>Conclusion: </strong>The MSRSGC is useful in WT diagnostics, and it improves communication between cytopathologists and clinicians. In this study, the most useful cytomorphological features that led to accurate WT diagnoses was papillary architecture in cell block specimens and the most significant pitfall was necrosis followed by diffuse hypercellularity.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-19"},"PeriodicalIF":1.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Message from the International Academy of Cytology.","authors":"","doi":"10.1159/000543694","DOIUrl":"https://doi.org/10.1159/000543694","url":null,"abstract":"","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":"69 1","pages":"105-106"},"PeriodicalIF":1.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Message from the International Academy of Cytology.","authors":"","doi":"10.1159/000543460","DOIUrl":"https://doi.org/10.1159/000543460","url":null,"abstract":"","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":"69 1","pages":"104"},"PeriodicalIF":1.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPV Primary Screening and Collaboration in Quality Assurance Work between laboratories.","authors":"Henrik Edvardsson, Joakim Dillner","doi":"10.1159/000544988","DOIUrl":"https://doi.org/10.1159/000544988","url":null,"abstract":"<p><strong>Background: </strong>Cervical screening is changing to the use of Human Papillomavirus (HPV) testing as the primary screening test. It is essential that the well-established and critically important systems for quality assurance based on laboratory audit of seemingly negative samples taken before HSIL and cervical cancer are maintained. They provide a means of verifying if the actual screening is effective for the intended purpose. Together with international proficiency panels, audits provide a simple and unambiguous way to evaluate if the screening is adequate. Detailed knowledge how these systems work and how they are dependent on genotyping of HPV, biobanking and screening registries are vital to cytologists and pathologists involved in quality assurance work and follow-up of cervical lesions and cervical cancer. Interpretation and communication of outcome and results are equally important for a successful quality assurance work and should ideally be done together with expertise in HPV.</p><p><strong>Summary: </strong>The internationally defined procedures for laboratory audit, similar to those used for cytology, require sensitivities before HSIL of >95% and before invasive cervical cancer of >90%. If also results on blinded proficiency panels and international criteria for analytic sensitivity, specificity and reproducibility are achieved, the HPV screening test can be said to be adequate.</p><p><strong>Key messages: </strong>Performance of HPV screening tests in a cervical screening program includes similar laboratory audits as hitherto used for cytology. Similarly, technical proficiency of a laboratory is established using blinded proficiency panels with defined contents of virus. Detailed knowledge of quality assurance work is necessary for cytologists and pathologists. Communication of outcome and results depend on collaboration between laboratories.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-11"},"PeriodicalIF":1.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect on DNA stability of a novel pellet storing method for liquid-based cytology specimens fixed using an alcohol-based preservation solution: Studies using EGFR mutation detection on a lung adenocarcinoma cell line.","authors":"Yukiko Matsuo, Tsutomu Yoshida, Kazuya Yamashita, Yukitosh Satoh","doi":"10.1159/000545062","DOIUrl":"https://doi.org/10.1159/000545062","url":null,"abstract":"<p><strong>Introduction: </strong>Liquid-based cytology (LBC) specimens of lung cancer are increasingly being used for genetic analyses. Preservation conditions of specimens until DNA extraction are important because they can affect DNA quality. We investigated whether a novel method of storing residual LBC specimens as pellets using an alcohol-based preservation solution would improve DNA stability.</p><p><strong>Methods: </strong>Lung adenocarcinoma cell line cells fixed in PreservCyt® solution were either stored using the conventional method (suspended in PreservCyt®; Susp group) or washed in phosphate-buffered saline and stored as cell pellet (novel method; cell pellet [CP] group). We analyzed the DNA quality of the cells after storage at ambient temperatures for 7, 14, and 28 days and compared DNA stability in dry cell pellets (d-CP) versus wet cell pellets (CP) after 7 days of storage. DNA stability was evaluated based on epidermal growth factor receptor mutation detection efficiency using the Cycleave PCR method.</p><p><strong>Results: </strong>The dsDNA yield and DNA integrity number (DIN) in the CP group were significantly higher than those in the Susp group at all time points. However, the UV absorbance of DNA from the CP group was lower than that from the Susp group. Mutation detection analysis indicated that DNA from the CP group had significantly lower Ct values than that from the Susp group on days 14. The DIN of DNA from the d-CP group was comparable to that from the CP group; however, the dsDNA yield in the d-CP group was reduced to less than half.</p><p><strong>Conclusion: </strong>The storage of LBC specimens as cell pellets after fixation in alcohol-based preservation solutions offers improved DNA stability and is a promising strategy for genetic analysis.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-19"},"PeriodicalIF":1.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Accuracy and Risk of Malignancy in Salivary Lesions Reclassified Using the Revised Milan System: A Single Institution Retrospective Analysis.","authors":"Amrutha Aravind, Neha Kawatra Madan, Meetu Agrawal, Charanjeet Ahluwalia, Sana Ahuja","doi":"10.1159/000545111","DOIUrl":"https://doi.org/10.1159/000545111","url":null,"abstract":"<p><strong>Background: </strong>Salivary gland tumors represent 3% of head and neck neoplasms. Fine needle aspiration cytology (FNAC) is widely used for preoperative diagnosis, but its accuracy varies due to the diverse morphology of these tumors. The revised 2023 Milan System for Reporting Salivary Gland Cytopathology (MSRSGC) provides updated risk of malignancy (ROM) estimates and guidelines for standardized reporting. This study reclassifies salivary gland FNAs using the revised MSRSGC and evaluates the ROM and diagnostic accuracy of each category.</p><p><strong>Materials and methods: </strong>We retrospectively analyzed 184 FNAC samples of salivary gland lesions collected from January 2023 to June 2024. Each case was reclassified according to the 2023 Milan System. Histopathological follow-up was available for 46 cases. ROM was calculated for each category, and sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and diagnostic accuracy were computed for two diagnostic groups: Group A (suspicious for malignancy and malignant) and Group B (malignant only).</p><p><strong>Results: </strong>Of the 184 cases, 47.3% were benign and 14.1% were malignant. The ROM for non-diagnostic, non-neoplastic, atypia of undetermined significance (AUS), benign, SUMP, suspicious for malignancy, and malignant categories were 33%, 0%, 50%, 7.1%, 0%, 66.6%, and 100%, respectively. Group B had higher specificity and PPV, while Group A showed improved sensitivity and diagnostic accuracy.</p><p><strong>Conclusion: </strong>The revised Milan System improves diagnostic accuracy in salivary gland FNAs. However, variations in ROM highlight the need for further studies to refine diagnostic challenges in certain categories.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-12"},"PeriodicalIF":1.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Criteria of Bile Cytology: A Multicenter Comparative Study of Conventional and Liquid-Based Cytology in Japan.","authors":"Yoshiki Naito, Akihiko Kawahara, Ryo Makino, Takako Tokumitsu, Daisuke Nagayama, Eiji Sadashima, Kaori Nakamura, Ryoji Nishizaki, Tadasuke Nagatomo, Yukina Fujino, Chie Hayakawa, Keishi Mizuguchi, Tatsuya Mori, Kenichi Hirabayashi, Takuma Tajiri","doi":"10.1159/000544997","DOIUrl":"https://doi.org/10.1159/000544997","url":null,"abstract":"<p><strong>Introduction: </strong>The use of liquid-based cytology (LBC) in non-gynecological fields has progressively increased. However, studies focusing on the application of LBC in bile cytology are limited, and its efficacy remains uncertain. In this study, we assessed the potential of LBC in bile cytology by evaluating the interobserver agreement.</p><p><strong>Methods: </strong>Bile cytology specimens were collected between 2015 and 2022 by using endoscopic retrograde cholangiopancreatography. Eleven cytotechnologists participated in the evaluation. Digital images of bile cytology specimens prepared using conventional smear (CS), ThinPrep LBC (TP-LBC), and SurePath LBC (SP-LBC) methods (20 cases per preparation method) were assessed for interobserver agreement on 21 diagnostic criteria. The diagnostic accuracy was evaluated using 50 bile cytology cases per preparation method.</p><p><strong>Results: </strong>High interobserver agreement (exact kappa > 0.6) was observed for features, including the loss of nuclear polarity, irregular nuclear spacing, nuclear size variation, and increased nuclear-to-cytoplasmic ratio across the CS, TP-LBC, and SP-LBC methods. Malignant cells in TP-LBC appeared smaller, rounder, and formed flat aggregates compared with those in CS. In contrast, the malignant cells in SP-LBC formed three-dimensional clusters that overlapped and exhibited higher cellularity than those in CS. The sensitivity of bile cytology was 76.4%, 84.8%, and 93.2% for CS, TP-LBC, and SP-LBC, respectively.</p><p><strong>Conclusion: </strong>The observers consistently recognized malignant cell features in bile cytology, irrespective of the preparation method. Understanding common features and method-specific cellular morphology is crucial for enhancing diagnostic accuracy. Our findings suggest that LBC methods can be effectively applied to bile cytology, potentially offering improved diagnostic accuracy compared with conventional methods.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-18"},"PeriodicalIF":1.6,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytological Diagnosis by Fine-Needle Aspiration or Core Biopsy with Touch Preparation for Renal Cystic or Solid Lesions: A Single-Center Clinicopathological Analysis.","authors":"Jiayan Liu, Changwei Yang, Xiaohui Wu, Li Yang, Hong Xu","doi":"10.1159/000543822","DOIUrl":"10.1159/000543822","url":null,"abstract":"<p><strong>Introduction: </strong>A retrospective study analyzed real-life data from a single-center cohort of renal fine-needle aspiration (FNA) and core biopsy (CB) with touch preparation (TP) procedures over an 18-year period and aimed to provide a comprehensive overview of the evaluation of cytological diagnostic performance, challenges, and accuracy concerning renal cystic, solid, and mixed cystic-solid lesions.</p><p><strong>Methods: </strong>All percutaneous ultrasound-guided FNA and CT-guided CB with TP performed at our institution from 2006 to 2024 were identified.</p><p><strong>Results: </strong>A total of 89 renal cytology procedures were performed during the study period. Sixty-two (69.7%) lesions displayed cystic radiological features, 20 (22.5%) lesions presented solid mass, and only 7 (7.8%) lesions exhibited mixed cystic-solid radiological features. Of the procedures performed, seventy-five (84.3%) were ultrasound-guided FNA biopsies, while 14 (15.7%) were CT-guided CB with TP. Of the 62 renal cystic lesions, 54 (87.1%) were simple renal cysts. Twelve (60%) in 20 solid lesions were malignant, mainly involving clear cell renal cell carcinoma (RCC), urothelial carcinoma, and collecting duct carcinoma. Cytological diagnoses of renal mixed cystic-solid lesions mainly involved tuberculosis and clear cell RCC. However, only 22 cases had corresponding histopathological specimens available for comparison. The concordance rate between cytological diagnoses and surgical pathology specimens for cystic, solid, and mixed cystic-solid renal lesions was 100%, 92.3%, and 80%, respectively.</p><p><strong>Conclusion: </strong>In our series, FNA or CB with TP demonstrates significant diagnostic utility in the evaluation of renal lesions. The diagnostic accuracy of FNA cytology for renal lesions has been enhanced through the application of immunocytochemical staining on cell blocks.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-11"},"PeriodicalIF":1.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}