Acta Cytologica最新文献

{"title":"Effect on DNA stability of a novel pellet storing method for liquid-based cytology specimens fixed using an alcohol-based preservation solution: Studies using EGFR mutation detection on a lung adenocarcinoma cell line.","authors":"Yukiko Matsuo, Tsutomu Yoshida, Kazuya Yamashita, Yukitosh Satoh","doi":"10.1159/000545062","DOIUrl":"https://doi.org/10.1159/000545062","url":null,"abstract":"<p><strong>Introduction: </strong>Liquid-based cytology (LBC) specimens of lung cancer are increasingly being used for genetic analyses. Preservation conditions of specimens until DNA extraction are important because they can affect DNA quality. We investigated whether a novel method of storing residual LBC specimens as pellets using an alcohol-based preservation solution would improve DNA stability.</p><p><strong>Methods: </strong>Lung adenocarcinoma cell line cells fixed in PreservCyt® solution were either stored using the conventional method (suspended in PreservCyt®; Susp group) or washed in phosphate-buffered saline and stored as cell pellet (novel method; cell pellet [CP] group). We analyzed the DNA quality of the cells after storage at ambient temperatures for 7, 14, and 28 days and compared DNA stability in dry cell pellets (d-CP) versus wet cell pellets (CP) after 7 days of storage. DNA stability was evaluated based on epidermal growth factor receptor mutation detection efficiency using the Cycleave PCR method.</p><p><strong>Results: </strong>The dsDNA yield and DNA integrity number (DIN) in the CP group were significantly higher than those in the Susp group at all time points. However, the UV absorbance of DNA from the CP group was lower than that from the Susp group. Mutation detection analysis indicated that DNA from the CP group had significantly lower Ct values than that from the Susp group on days 14. The DIN of DNA from the d-CP group was comparable to that from the CP group; however, the dsDNA yield in the d-CP group was reduced to less than half.</p><p><strong>Conclusion: </strong>The storage of LBC specimens as cell pellets after fixation in alcohol-based preservation solutions offers improved DNA stability and is a promising strategy for genetic analysis.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-19"},"PeriodicalIF":1.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Accuracy and Risk of Malignancy in Salivary Lesions Reclassified Using the Revised Milan System: A Single Institution Retrospective Analysis.","authors":"Amrutha Aravind, Neha Kawatra Madan, Meetu Agrawal, Charanjeet Ahluwalia, Sana Ahuja","doi":"10.1159/000545111","DOIUrl":"https://doi.org/10.1159/000545111","url":null,"abstract":"<p><strong>Background: </strong>Salivary gland tumors represent 3% of head and neck neoplasms. Fine needle aspiration cytology (FNAC) is widely used for preoperative diagnosis, but its accuracy varies due to the diverse morphology of these tumors. The revised 2023 Milan System for Reporting Salivary Gland Cytopathology (MSRSGC) provides updated risk of malignancy (ROM) estimates and guidelines for standardized reporting. This study reclassifies salivary gland FNAs using the revised MSRSGC and evaluates the ROM and diagnostic accuracy of each category.</p><p><strong>Materials and methods: </strong>We retrospectively analyzed 184 FNAC samples of salivary gland lesions collected from January 2023 to June 2024. Each case was reclassified according to the 2023 Milan System. Histopathological follow-up was available for 46 cases. ROM was calculated for each category, and sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and diagnostic accuracy were computed for two diagnostic groups: Group A (suspicious for malignancy and malignant) and Group B (malignant only).</p><p><strong>Results: </strong>Of the 184 cases, 47.3% were benign and 14.1% were malignant. The ROM for non-diagnostic, non-neoplastic, atypia of undetermined significance (AUS), benign, SUMP, suspicious for malignancy, and malignant categories were 33%, 0%, 50%, 7.1%, 0%, 66.6%, and 100%, respectively. Group B had higher specificity and PPV, while Group A showed improved sensitivity and diagnostic accuracy.</p><p><strong>Conclusion: </strong>The revised Milan System improves diagnostic accuracy in salivary gland FNAs. However, variations in ROM highlight the need for further studies to refine diagnostic challenges in certain categories.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-12"},"PeriodicalIF":1.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Criteria of Bile Cytology: A Multicenter Comparative Study of Conventional and Liquid-Based Cytology in Japan.","authors":"Yoshiki Naito, Akihiko Kawahara, Ryo Makino, Takako Tokumitsu, Daisuke Nagayama, Eiji Sadashima, Kaori Nakamura, Ryoji Nishizaki, Tadasuke Nagatomo, Yukina Fujino, Chie Hayakawa, Keishi Mizuguchi, Tatsuya Mori, Kenichi Hirabayashi, Takuma Tajiri","doi":"10.1159/000544997","DOIUrl":"https://doi.org/10.1159/000544997","url":null,"abstract":"<p><strong>Introduction: </strong>The use of liquid-based cytology (LBC) in non-gynecological fields has progressively increased. However, studies focusing on the application of LBC in bile cytology are limited, and its efficacy remains uncertain. In this study, we assessed the potential of LBC in bile cytology by evaluating the interobserver agreement.</p><p><strong>Methods: </strong>Bile cytology specimens were collected between 2015 and 2022 by using endoscopic retrograde cholangiopancreatography. Eleven cytotechnologists participated in the evaluation. Digital images of bile cytology specimens prepared using conventional smear (CS), ThinPrep LBC (TP-LBC), and SurePath LBC (SP-LBC) methods (20 cases per preparation method) were assessed for interobserver agreement on 21 diagnostic criteria. The diagnostic accuracy was evaluated using 50 bile cytology cases per preparation method.</p><p><strong>Results: </strong>High interobserver agreement (exact kappa > 0.6) was observed for features, including the loss of nuclear polarity, irregular nuclear spacing, nuclear size variation, and increased nuclear-to-cytoplasmic ratio across the CS, TP-LBC, and SP-LBC methods. Malignant cells in TP-LBC appeared smaller, rounder, and formed flat aggregates compared with those in CS. In contrast, the malignant cells in SP-LBC formed three-dimensional clusters that overlapped and exhibited higher cellularity than those in CS. The sensitivity of bile cytology was 76.4%, 84.8%, and 93.2% for CS, TP-LBC, and SP-LBC, respectively.</p><p><strong>Conclusion: </strong>The observers consistently recognized malignant cell features in bile cytology, irrespective of the preparation method. Understanding common features and method-specific cellular morphology is crucial for enhancing diagnostic accuracy. Our findings suggest that LBC methods can be effectively applied to bile cytology, potentially offering improved diagnostic accuracy compared with conventional methods.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-18"},"PeriodicalIF":1.6,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Thyroid Fine-Needle Aspirates Using 2023 Bethesda System.","authors":"Niti Sureka, Charanjeet Ahluwalia, Sana Ahuja, Neha Kawatra Madan, Meetu Agrawal, Sunil Ranga","doi":"10.1159/000544807","DOIUrl":"10.1159/000544807","url":null,"abstract":"<p><strong>Introduction: </strong>Thyroid nodules are common, with about 5% being malignant, necessitating accurate evaluation to avoid unnecessary surgeries. Fine-needle aspiration cytology (FNAC) is a crucial diagnostic tool, and the 2023 Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) refines FNAC's diagnostic accuracy and risk stratification for thyroid lesions.</p><p><strong>Materials and methods: </strong>This retrospective study reclassified thyroid FNAs performed at a tertiary care centre from January 2023 to June 2024 using the 2023 TBSRTC and compared with available histopathological diagnoses. FNAC slides were reviewed and reclassified according to the 2023 TBSRTC categories by two cytopathologists. Statistical analysis included sensitivity, specificity, positive predictive value, negative predictive value, and overall diagnostic accuracy.</p><p><strong>Results: </strong>The study included 387 patients (female-to-male ratio of 4:1), with 8% non-diagnostic, 61.5% benign, 9% AUS, 7.5% FN, 8% SFM, and 6% malignant cases. Histopathological correlation was available for 190 cases, revealing 126 benign and 64 malignant lesions. Considering NIFTP as benign on histopathology, the ROM for benign, FN, and SFM categories was 10%, 38%, and 69.2%, respectively. The highest sensitivity (88.2%) was achieved when AUS, FN, SFM, and malignant categories were considered positive for malignancy. The highest diagnostic accuracy (85.4%) was observed when SFM and malignant categories were considered positive.</p><p><strong>Conclusion: </strong>FNAC, guided by the 2023 TBSRTC, is a reliable diagnostic tool for thyroid lesions, offering high sensitivity and specificity.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-11"},"PeriodicalIF":1.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study Designs in Cytological Research: Impact in Evidence-Based Medicine.","authors":"Kari Syrjänen","doi":"10.1159/000544851","DOIUrl":"10.1159/000544851","url":null,"abstract":"","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-7"},"PeriodicalIF":1.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytological Diagnosis by Fine-Needle Aspiration or Core Biopsy with Touch Preparation for Renal Cystic or Solid Lesions: A Single-Center Clinicopathological Analysis.","authors":"Jiayan Liu, Changwei Yang, Xiaohui Wu, Li Yang, Hong Xu","doi":"10.1159/000543822","DOIUrl":"10.1159/000543822","url":null,"abstract":"<p><strong>Introduction: </strong>A retrospective study analyzed real-life data from a single-center cohort of renal fine-needle aspiration (FNA) and core biopsy (CB) with touch preparation (TP) procedures over an 18-year period and aimed to provide a comprehensive overview of the evaluation of cytological diagnostic performance, challenges, and accuracy concerning renal cystic, solid, and mixed cystic-solid lesions.</p><p><strong>Methods: </strong>All percutaneous ultrasound-guided FNA and CT-guided CB with TP performed at our institution from 2006 to 2024 were identified.</p><p><strong>Results: </strong>A total of 89 renal cytology procedures were performed during the study period. Sixty-two (69.7%) lesions displayed cystic radiological features, 20 (22.5%) lesions presented solid mass, and only 7 (7.8%) lesions exhibited mixed cystic-solid radiological features. Of the procedures performed, seventy-five (84.3%) were ultrasound-guided FNA biopsies, while 14 (15.7%) were CT-guided CB with TP. Of the 62 renal cystic lesions, 54 (87.1%) were simple renal cysts. Twelve (60%) in 20 solid lesions were malignant, mainly involving clear cell renal cell carcinoma (RCC), urothelial carcinoma, and collecting duct carcinoma. Cytological diagnoses of renal mixed cystic-solid lesions mainly involved tuberculosis and clear cell RCC. However, only 22 cases had corresponding histopathological specimens available for comparison. The concordance rate between cytological diagnoses and surgical pathology specimens for cystic, solid, and mixed cystic-solid renal lesions was 100%, 92.3%, and 80%, respectively.</p><p><strong>Conclusion: </strong>In our series, FNA or CB with TP demonstrates significant diagnostic utility in the evaluation of renal lesions. The diagnostic accuracy of FNA cytology for renal lesions has been enhanced through the application of immunocytochemical staining on cell blocks.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-11"},"PeriodicalIF":1.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid On-Site Evaluation with Pancreatic Fine-Needle Biopsies: Successes and Challenges.","authors":"T Leif Helland, M Lisa Zhang, Martha B Pitman, Vanda F Torous","doi":"10.1159/000544737","DOIUrl":"10.1159/000544737","url":null,"abstract":"<p><strong>Background: </strong>Endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) using larger, next-generation cutting needles is a minimally invasive method for the diagnosis of pancreatic lesions. Rapid on-site evaluation (ROSE) is employed to render preliminary diagnoses, ensure specimen adequacy, and triage tissue for ancillary testing and can be performed on FNB cores. Given the difficulty of pancreatic cytology and the novelty of ROSE with these larger cutting needles, this study was performed to evaluate discrepancies between ROSE and the final diagnosis to uncover challenging diagnostic areas.</p><p><strong>Methods: </strong>Final reports from pancreatic FNBs with ROSE between January 2019 and December 2021 were reviewed, and the ROSE and final diagnoses were compared. Cases were categorized into nondiagnostic (ND), negative for malignancy (NEG), atypical, neoplastic (NEO), suspicious for malignancy (SFM), and positive for malignant cells (POS). A major discrepancy was defined as an ND/NEG versus NEO/SFM/POS interpretation.</p><p><strong>Results: </strong>A total of 454 cases were identified. The ROSE versus final diagnosis breakdown was as follows: ND/NEG 18.7% versus 16.3%, atypical 6.4% versus 5.1%, NEO 10.8% versus 11.9%, SFM 4.4% versus 2.0%, and POS 59.7% versus 64.8%. The concordance rate was high at 96.9% with only 14 (3.1%) major discrepancies, which included 6 due to interpretive error, 3 due to sampling error, and 5 due to a combination of both. While the majority of lesions in the cohort were conventional ductal adenocarcinomas (76%), there was an over-representation of non-ductal tumors constituting major discrepancies (6/14; 42.9%).</p><p><strong>Conclusions: </strong>ROSE using pancreatic EUS-FNB is possible and provides an accurate interpretation in most cases. Diagnostic challenges remain with non-ductal tumors.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-13"},"PeriodicalIF":1.6,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oncocytic Tumors in the Thyroid: A Tri-Focal Review - Integrated Cytopathological, Pathological, and Molecular Perspectives.","authors":"Maria A Gubbiotti, Sule Canberk, Zubair W Baloch","doi":"10.1159/000544739","DOIUrl":"10.1159/000544739","url":null,"abstract":"<p><strong>Background: </strong>The thyroid gland is a treasure trove of pathology ranging from the benign to the overtly malignant. Both neoplastic and nonneoplastic thyroid lesions can exhibit oncocytic change. Here we present an overview of cytologic and histopathologic findings encountered in these oncocytic neoplasms with a focus on the molecular aspects that drive their tumorigenesis.</p><p><strong>Summary: </strong>Oncocytic change is unique to a subset of thyroid lesions ranging from nonneoplastic nodular hyperplasia to high-grade malignancy. It can also be encountered in non-follicular-derived neoplasms as well as in the adjacent parathyroid glands. At the genetic level, these lesions demonstrate a different genetic signature from classic follicular-derived lesions, often involving alterations of mitochondrial genes.</p><p><strong>Key messages: </strong>Oncocytic change can be seen in nonneoplastic and neoplastic thyroid pathology. Rarely, oncocytic change can be seen in medullary thyroid carcinoma and certain subtypes of papillary thyroid carcinoma as well as the parathyroid gland. Oncocytic neoplasms of the thyroid harbor molecular alterations often involving mitochondrial genes, which is distinct from other thyroid neoplasia.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-17"},"PeriodicalIF":1.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utility of p16INK4a Staining on Cell Blocks Prepared from Residual Liquid-Based Cervicovaginal Material.","authors":"Shabana Andleeb Ansari, Anshu","doi":"10.1159/000544071","DOIUrl":"10.1159/000544071","url":null,"abstract":"<p><strong>Introduction: </strong>Cervical cancer screening using Pap smears is affected by false-negative results. Liquid-based cytology (LBC) offers the technical advantage of preparing cell blocks from residual fluid to conduct ancillary tests on them. The p16INK4a gene product has been shown to be strongly overexpressed in dysplastic cervical epithelia and serves as surrogate marker for high-risk human papilloma virus infection.</p><p><strong>Materials and methods: </strong>Microwave-processed cell blocks were prepared from residual material in vials after ThinPrep slide preparation, stained with hematoxylin and eosin and p16INK4a. Nuclear staining with or without cytoplasmic staining on p16 slides was considered positive. Four parameters were evaluated: percentage of positive cells, intensity of staining, number of positively stained cells in close contact, and full-thickness epithelial staining. We compared sensitivity and specificity of ThinPrep smears and p16-stained cell blocks in diagnosing invasive malignancy.</p><p><strong>Results: </strong>The intensity and percentage of p16-positive cells was found to increase with increasing grade of cervical abnormality. We found good concordance between ThinPrep smear and cell block diagnoses in cases which were negative for intraepithelial lesion or malignancy (97.6%), in low-grade squamous intraepithelial lesions (90%), high-grade squamous intraepithelial lesions (100%), and squamous cell carcinomas (93.5%). Of 16 discrepant cases, 9 were reported unsatisfactory on ThinPrep smears due to abundant necrosis or scant cellularity. All these turned out to have malignancies on follow-up and review of histology. The sensitivity of ThinPrep and p16-stained cell blocks in diagnosing invasive malignancy were 70.2% and 85.1%, respectively, while the specificity of both was 100%.</p><p><strong>Conclusions: </strong>Cell blocks prepared from residual fluid in LBC vials have the potential to reduce the rates of inadequacy and are feasible in routine practice. While the cost of p16 on cell blocks may be too prohibitive for use in routine cervical screening programs, if used judiciously in combination with clinical suspicion, a lot of valuable material which is usually discarded in the residual LBC vials can prove to be crucial in arriving at the correct diagnosis.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-11"},"PeriodicalIF":1.6,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applicability of Artificial Intelligence Analysis in Oral Cytopathology: A Pilot Study.","authors":"Igor Cavalcante Guedes, Ana Laura Ferrares Espinosa, Tatiana Wannmacher Lepper, Maikel Maciel Rönnau, Natália Batista Daroit, Manuel M Oliveira, Pantelis Varvaki Rados","doi":"10.1159/000543852","DOIUrl":"10.1159/000543852","url":null,"abstract":"<p><strong>Introduction: </strong>Oral cancer, especially oral squamous cell carcinoma (OSCC), is a global health challenge due to factors such as late detection and high mortality rates. Early detection is essential through monitoring by healthcare professionals. Cytopathology is a cellular analysis model for evaluating cellular damage preceding the clinical appearance of OSCC, but it requires training and has diagnostic limitations, due to its subjective aspect. Artificial intelligence (AI) shows potential to enhance the interpretation of cytological images, reducing working time and subjectivity.</p><p><strong>Objective: </strong>The aim of the study was to compare the effectiveness of human analyses versus AI system assessment of oral cell smears stained by the Papanicolaou technique.</p><p><strong>Methodology: </strong>The study comprised 57 patients in Porto Alegre - RS divided into four groups: control group (CG), exposed group (EG), oral potentially malignant disorders group (OPMDG), and OSCC group (OSCCG). Cytopathological smears were collected from the border of the tongue of CG and EG and from the lesional area in OSCCG and OPMDG. The Papanicolaou technique was performed according to standard protocol, with morphological analysis. Images were analyzed by two human examiners as well as by an AI system (Papanicolaou Slide Image Examiner [PSIE]).</p><p><strong>Results: </strong>Concordance between human and PSIE was good. The proportion of cytological findings between human and PSIE was similar, and the analysis time of PSIE was 16.6 times shorter than that of human researchers.</p><p><strong>Conclusion: </strong>The use of AI for OSCC screening is promising and demonstrated to be a suitable tool for routine use mainly with the advance of IA-human concordance analysis and serving as a tool to accelerate the analytical process.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-8"},"PeriodicalIF":1.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}