Acta Cytologica最新文献

{"title":"Comprehensive Study of Thyroid Fine Needle Aspiration in Pediatric and Young Adults.","authors":"Erika F Rodriguez, Precious Ann V Fortes, Victoria Lee, Jeffrey D Goldstein, Neda A Moatamed","doi":"10.1159/000546006","DOIUrl":"https://doi.org/10.1159/000546006","url":null,"abstract":"<p><strong>Introduction: </strong>Thyroid nodules are uncommon in the pediatric population, with a 1-1.7% prevalence. The Bethesda System of Reporting Thyroid Cytopathology (TBSTC) is a well-established thyroid fine needle aspiration (FNA) reporting system. While the TBSTC guides therapy for both adult and pediatric patients, the reported risk of malignancy (ROM) is variable in the literature. The aim of this study is to compare the ROM in pediatric age of <15 with. ≥15 years old.</p><p><strong>Material and methods: </strong>We searched for patients aged 21 or younger who underwent FNA of thyroid nodules from 2016-2021. Data included patient demographics, nodule size, FNA results, molecular results, and surgical pathology follow-up. Patients were divided into two cohorts: 0-14 (<15) and 15-21 (≥15) years old.</p><p><strong>Results: </strong>145 nodules from 102 patients (26 cases in <15 and 94 in patients ≥ 15) were analyzed. Diagnoses and ROM were: non-diagnostic (n=3); benign (108, ROM 50%), atypia of unknown significance (n=13, ROM 67%), follicular neoplasm (n= 6, ROM 33%), suspicious for malignancy (n=1, ROM 100%), malignant (n=14 ROM 100%). No significant differences (p values ≥ 0.2) between the age groups were noted. Based on surgical follow-up results, the overall malignancy rate was 8% and 19% for <15 and ≥15 years old groups, respectively.</p><p><strong>Conclusion: </strong>The ROM for thyroid nodules in the pediatric population is higher than in adults. There appears to be a trend towards a higher overall malignancy rate in patients aged 15-21 compared to those under 15, though this difference is not statistically significant. Further studies with larger patient numbers are required to determine if the ROM differs significantly between these age groups.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-16"},"PeriodicalIF":1.6,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oncocytic Tumors in the Kidney: A Tri-Focal Review - Integrated Pathological, Cytopathological, and Molecular Perspectives, Part 2.","authors":"Laura Lanteri, Elena Luppi, Alessia Cimadamore, Gladell P Paner, José A Jiménez Heffernan, Giuseppe Gasparre","doi":"10.1159/000545946","DOIUrl":"10.1159/000545946","url":null,"abstract":"<p><strong>Background: </strong>This second of two parts review is devoted to the practical aspects of fine needle aspiration biopsy diagnosis of renal oncocytoma and the interesting biology underlying the morphologic transformation of oncocytes.</p><p><strong>Summary: </strong>In the first section, we describe the most useful cytologic variables for the recognition of oncocytoma since its first cytologic description 44 years ago. The usefulness of the recently introduced cytologic diagnostic category of \"low-risk oncocytic neoplasm\" is discussed, as well as the known problems of differential diagnosis. The second section deals with the molecular aspects of oncocytes, with special emphasis on correlating it with the peculiar morphology of oncocytic tumors and their less aggressive behavior. First, why does this accumulation of abnormal mitochondria occur, and second, what are the consequences? Regarding oxidative phosphorylation, oncocytes show a dysfunctional respiratory complex that makes them unable to respond adequately to the hypoxia so typical of the neoplastic environment.</p><p><strong>Key messages: </strong>The low-risk oncocytic neoplasm category is so relevant that they may limit the possibility of an accurate diagnosis in small specimens, such as FNA and core biopsies. However, this must be compatible with the possibility of making a useful diagnosis for the therapeutic management of the patient. Further, we discuss the genes and molecules responsible for mitochondrial dysfunction, and, finally, the molecular differences between sporadic oncocytomas and those associated with a hereditary context.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-12"},"PeriodicalIF":1.6,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Performance of the Milan System for Reporting Salivary Gland Cytopathology and a Proposed Algorithm for Fine-Needle Aspiration Cytology of Salivary Gland Lesions.","authors":"Norihide Mochizuki, Hirotaka Fujita, Takuma Tajiri, Masataka Ueda, Makiko Kurata, Chie Inomoto, Tomoko Sugiyama, Daisuke Maki, Shuichi Shiraishi, Tomohisa Machida, Hitoshi Ito, Yohei Masugi, Naoya Nakamura","doi":"10.1159/000546005","DOIUrl":"https://doi.org/10.1159/000546005","url":null,"abstract":"<p><strong>Introduction: </strong>We evaluated concordance between Milan System for Reporting Salivary Gland Cytopathology (MSRSGC)-based categorization of salivary gland masses/lesions screened by fine-needle aspiration cytology (FNAC) and final histopathologic diagnoses, aiming to identify factors predictive of concordance, with the goal of appropriate case management.</p><p><strong>Methods: </strong>The study was retrospective and involved 101 cases of salivary mass/lesion examined by FNAC. We compared MSRSGC categories against the final histopathologic classes (non-neoplasm, benign neoplasm, or malignant neoplasm) and calculated diagnostic concordance in each class. Concordance was defined as: MSRSGC categorization of a lesion as a category Ⅱ lesion and a histopathologic classification as a non-neoplasm; MSRSGC categorization of a lesion as a category Ⅳ-A lesion and a histopathologic classification as a benign neoplasm; or MSRSGC categorization of a lesion as a category Ⅴ or Ⅵ lesion and a histopathologic classification as a malignant neoplasm. We then compared clinicopathologic factors between concordant and discordant cases.</p><p><strong>Results: </strong>Diagnostic concordance for non-neoplasms, benign neoplasms, malignant neoplasms, and total cases was 81.8% (9/11), 81.7% (58/71), 66.6% (8/12), and 79.8% (75/94), respectively, with no significant between-class difference. We found the shortest distance from the body surface to the salivary lesion differed significantly between the concordant group and the discordant group (5.35 mm vs. 7.30 mm), and the optimal cut-off was determined to be 8.00 mm (P < 0.01).</p><p><strong>Conclusion: </strong>Based on the distance of either ＜８mm or ≧8mm from the body surface to the mass/lesion, we believe our proposed FNAC algorithm of treatment strategies is a reliable guide for otolaryngologists on evaluating salivary gland lesions.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-20"},"PeriodicalIF":1.6,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oncocytic Tumors in the Kidney: A Tri-Focal Review - Integrated Pathological, Cytopathological, and Molecular Perspectives. Part 1.","authors":"Alessia Cimadamore, Carmine Franzese, José A Jiménez Heffernan, Rodolfo Montironi, Jung Woo Kwon, Giuseppe Gasparre, Gladell P Paner","doi":"10.1159/000545812","DOIUrl":"https://doi.org/10.1159/000545812","url":null,"abstract":"<p><p>We review the pathological, cytopathological and molecular features centered on renal oncocytoma and its differential diagnosis. The recent expansion of entities under the category of renal tumors with oncocytic or eosinophilic cytoplasm has important implications on how cytologic diagnosis is clinically considered. In this first of two parts, we discussed the pathological spectrum of oncocytic or eosinophilic tumors of the kidney that includes oncocytoma; chromophobe renal cell carcinoma (ChRCC) - including its eosinophilic variant (eosinophilic ChRCC); hybrid oncocytic/chromophobe tumors, either sporadic or syndromic; oncocytic papillary RCC, acquired cystic disease (ACD)-associated RCC; succinate dehydrogenase (SDH)-deficient RCC; and eosinophilic solid and cystic (ESC) RCC. We describe the histomorphological and immunohistochemical features of these tumors, including the newly accepted entities, and to focus on the molecular alterations reported. A practical approach for differential diagnosis and broader correlation to available cytologic findings are provided, with more in-depth cytologic descriptions for oncocytoma and eosinophilic ChRCC included in part two of this review. Most of the oncocytic tumors have an indolent behavior, although few aggressive cases have been reported in patients with ESC RCC, eosinophilic vacuolated tumor (EVT), and SDH-deficient RCC. In this era where surveillance management for low grade oncocytic renal tumors is considered, precise diagnosis is important as it will have an impact on their subsequent management. Further, accurate diagnosis is important especially in renal tumors associated with hereditary neoplasms for monitoring and genetic counseling for their family members.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-22"},"PeriodicalIF":1.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Decision-Making and Risk of Malignancy when Parotid Gland Fine Needle Aspiration Cytology Indicates a Non-Neoplastic or Non-Diagnostic Finding.","authors":"Mira Naukkarinen, Katri Aro, Jetta Kelppe, Minna Sirviö, Antti Mäkitie, Jussi Tarkkanen, Timo Atula","doi":"10.1159/000545145","DOIUrl":"10.1159/000545145","url":null,"abstract":"<p><strong>Introduction: </strong>Non-neoplastic and non-diagnostic cytological findings present a diagnostic and therapeutic challenge in head and neck oncology. Both groups still harbor a risk of malignancy (ROM). Of note, ROM values have been counted from surgically confirmed lesions only. The purpose of this study was to evaluate the clinical course of patients with non-neoplastic or non-diagnostic fine needle aspiration cytology (FNAC) findings from a parotid gland lesion.</p><p><strong>Methods: </strong>This retrospective cohort study comprises all 184 consecutive patients who visited the Department of Otorhinolaryngology - Head and Neck Surgery, Helsinki University Hospital (HUS, Helsinki, Finland) and whose first parotid gland FNAC result during 2016-2018 was non-neoplastic or non-diagnostic. The cytology reports were obtained from the HUS Pathological Archives (Q-Pati). Demographics, physical examination findings, and cytopathological and treatment data were reviewed. Two patient groups were formed according to their clinical management: those who had surgery and those who were only followed up. If the parotid gland was operated on, FNAC was compared with histology. If the patient was followed up without surgical treatment, the follow-up data included a review of the patient records supplemented with a questionnaire.</p><p><strong>Results: </strong>Altogether, there were 186 parotid lesions. Seventy-six (40.9%) tumors in 75 patients were operated on, and 110 (59.1%) were only followed up. Of all parotid gland lesions, 12 (6.5%) turned out to be malignant, and all of them were in the surgically treated group. When only followed up clinically, with repeated needle sampling or imaging during the minimum 4-year follow-up period, none of the other lesions turned out to be malignant.</p><p><strong>Conclusion: </strong>The ROM for non-diagnostic and non-neoplastic FNAC samples is lower when all FNAC samples, including also those from nonsurgically treated patients, are included in comparison with the series that includes only surgically treated patients with histopathological confirmation. Our results suggest that this patient group can be followed up conservatively in the absence of abnormal symptoms or radiological findings.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-11"},"PeriodicalIF":1.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oncocytic Adrenal Tumors: A Tri-Focal Review with Integrated Cytopathological, Pathological, and Molecular Perspectives.","authors":"Vincenzo Condello, Massimo Bongiovanni, C Christofer Juhlin","doi":"10.1159/000545715","DOIUrl":"10.1159/000545715","url":null,"abstract":"<p><strong>Background: </strong>Oncocytic lesions of the adrenal gland pose several diagnostic challenges as they can be associated with both functional and non-functional adrenal disorders and may be either benign or malignant.</p><p><strong>Summary: </strong>Oncocytic tumors are predominantly (>90%) composed of oncocytic cells, characterized by bulky, eosinophilic cytoplasm due to an abundance of mitochondria. Notably, the conventional histopathological criteria for diagnosing adrenal cortical carcinoma (ACC), such as the Weiss criteria, are not recommended for oncocytic tumors, and separate classification algorithms have been proposed for this entity. In addition to their unique cytopathology and histopathology, oncocytic adrenal cortical neoplasms share many driver gene alterations with conventional adrenal tumors, albeit at lower frequencies. However, these tumors also exhibit some distinct genetic changes, particularly deletions of mitochondrial DNA, which are consistent with patterns seen in oncocytic lesions of other endocrine organs. Interestingly, the presence of oncocytic features may correlate with prognosis in ACCs, making this morphological distinction clinically significant. Some studies suggest that oncocytic features could be linked to either a more favorable or unfavorable outcome, depending on other molecular markers. This highlights the importance of accurate diagnostic work-up for these lesions and underscores the critical role of endocrine pathologists in their management. While cytology is not part of the routine work-up for primary adrenal tumors, fine-needle aspiration cytology may still be useful in distinguishing primary adrenal tumors from metastases.</p><p><strong>Key messages: </strong>This review examines the histological and molecular characteristics of oncocytic adrenal cortical lesions, highlighting their clinically relevant differences from conventional adrenal tumors. It clarifies the limited role of cytology in diagnosing primary adrenal tumors while recognizing its usefulness in distinguishing adrenal metastases. Finally, it underscores the need for a tailored diagnostic approach to effectively manage this complex entity.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-11"},"PeriodicalIF":1.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oncocytic Tumors in the Familial and Syndromic Contexts: A Tri-Focal Review - Integrated Cytopathological, Pathological, and Molecular Perspectives.","authors":"Maria Cristina Riascos, Vania Nosé","doi":"10.1159/000545321","DOIUrl":"10.1159/000545321","url":null,"abstract":"<p><strong>Background: </strong>Familial neoplastic syndromes are distinguished by the presence of specific neoplasms which serve as critical indicators for their suspicion and diagnosis. Among these, only a limited subset includes tumors with distinctive oncocytic features, highlights the necessity for pathologists and clinicians to pursue further investigation in affected patients and their families.</p><p><strong>Summary: </strong>Advances in genetic research and diagnostic pathology have highlighted the germline predispositions underlying these tumors, which manifest across multiple organ systems, including thyroid, parathyroid, renal, and adrenal glands. This review examines the clinical, pathological, and molecular features of oncocytic neoplasms in the context of hereditary syndromes such as Carney complex, Li-Fraumeni syndrome, DICER1 syndrome, Birt-Hogg-Dubé syndrome, hyperparathyroidism-jaw tumor syndrome, hereditary leiomyomatosis and renal cell carcinoma syndrome, tuberous sclerosis syndrome, Beckwith-Wiedemann syndrome, and SDH-deficient hereditary paraganglioma/pheochromocytoma syndrome. It emphasizes the importance of recognizing syndromic associations through histopathological clues, genetic testing, and family history to facilitate accurate diagnosis and tailored management.</p><p><strong>Key message: </strong>By integrating clinical insights with molecular data, this paper sheds light on the mechanisms driving oncocytic transformation and underscores the role of pathologists in identifying hereditary cancer syndromes.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-14"},"PeriodicalIF":1.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving diagnostic accuracy of indeterminate thyroid fine needle aspiration using results of ultrasound examination, cyclin D1 immunostaining and molecular testing.","authors":"Shurong He, Jingxin Zhang, Mengge Wang, Feiliang Wang, Kan Gao, Rongming Chen, Songtao Hu, Jing Di, Dongge Liu, Mulan Jin","doi":"10.1159/000545319","DOIUrl":"https://doi.org/10.1159/000545319","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;Fine-needle aspiration (FNA) is the standard procedure for diagnosing thyroid nodules, however indeterminate cytological results present considerable challenges in clinical management. This study aimed to evaluate cyclin D1 immunocytochemistry and Chinese thyroid imaging reporting and data system (C-TIRADS) score, individually and in combination with a simple gene test for cytological diagnosis of Bethesda category Ⅲ‑Ⅴ.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A consecutive cohort of 177 thyroid FNA specimens with indeterminate diagnoses and available histopathologic follow‑up data were collected. The samples were evaluated by cyclin D1 immunocytochemistry and molecular testing for the BRAFV600E mutation or for a small panel of markers (BRAF, N‑RAS, H‑RAS, K‑RAS and TERT). Two experienced sonographers independently reviewed original sonographic images of each nodule and classified them using the C-TIRADS to reach a consensus score. We evaluated the optimal cut‑off points for cyclin D1 and C-TIRADS scores in diagnosing malignancy using receiver operating characteristic (ROC) curves and the area under the ROC curve (AUC). The specificity, sensitivity, positive predictive value (PPV) and negative predictive value (NPV) of all tests were evaluated with the crosstabs. Logistic regression analysis and ROC curve analysis were used to evaluate the diagnostic accuracy of the three tests individually and combination.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;We enrolled 169 patients (177 thyroid nodules), including 140 malignances, 15 low-risk neoplasms, and 22 benign lesions. All 177 specimens were tested for BRAF V600E, while only 21 specimens used the 5-gene detection protocol. With cut-off values set at 10% for cyclin D1 immunocytochemistry and at a C-TIRADS score of 3, along with defining a positive diagnosis as the presence of a mutation in genetic testing, the PPVs for diagnosing thyroid malignancy using the three tests were 97.8%, 90.3%, and 98% respectively; however, the NPVs were 50%, 24.2%, and 26.3 %. The sensitivities were 87.7%, 83.9%, and 63.9%, and the specificities were 86.4%, 36.4% and 90.9%. Regarding AUC, cyclin D1 alone demonstrated greater diagnostic accuracy (0.921, 95%CI=0.857-0.985, P=0.000) compared with the other two methods, which had AUC values of 0.775 (95%CI=0.703-0.846, P=0.000) and 0.587 (95%CI=0.443-0.731, P=0.235). The combination of two or three tests yielded higher accuracy (0.929, 95%CI=0.873-0.985, P=0.000; 0.925, 95%CI=0.860-0.989, P=0.000; 0.937, 95%CI=0.889-0.985, P=0.000) compared with cyclin D1 immunocytochemistry alone (0.921, 95%CI=0.857-0.985, P=0.000).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Adding cyclin D1 immunocytochemistry or a simple gene panel test, alone or in combination, significantly enhances the diagnostic value of the C-TIRADS score in thyroid nodules with indeterminate cytology. Laboratories should integrate these tests to effectively manage uncertain thyroid nodules and reduce diagnos","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-23"},"PeriodicalIF":1.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oncocytic Tumors in the Salivary Gland: Cytopathological, Pathological, and Molecular Features.","authors":"Alena Skálová, Martina Bradová, Arnaud Da Cruz Paula, William C Faquin","doi":"10.1159/000544802","DOIUrl":"10.1159/000544802","url":null,"abstract":"<p><strong>Background: </strong>Primary oncocytic salivary gland tumors and oncocytic subtypes of traditionally non-oncocytic salivary gland neoplasms are occasionally encountered in fine needle aspiration specimens, biopsies, and resections. Oncocytes are cells, either non-neoplastic or neoplastic, containing increased numbers of mitochondria resulting in cells with abundant eosinophilic cytoplasm and a low N/C ratio.</p><p><strong>Summary: </strong>A broad range of salivary gland tumors can be oncocytic including oncocytoma, Warthin tumor, mucoepidermoid carcinoma, salivary duct carcinoma, and others, especially those tumors where the oncocytic pattern represents a subtype of neoplasm; the oncocytic pattern can create a diagnostic challenge due to marked similarities in the oncocytic pattern of cells.</p><p><strong>Key messages: </strong>While their microscopic cytologic and histologic features may be similar, these tumors differ intrinsically at the molecular level. Ancillary studies such as immunologic (e.g., androgen receptor for salivary duct carcinoma) and molecular analysis, e.g., FISH for detecting the MAML2 or PLAG1/HMGA2 gene alterations in mucoepidermoid carcinoma and pleomorphic adenoma, respectively, can be used to classify these oncocytic tumors in difficult cases.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-12"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12060841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital Pathology in Real-Life Conditions for Daily Cytopathological Diagnosis: A Feasibility Study.","authors":"Ranya Soufan, Alicia Larive, Irène Villa, Anne Auperin, Jean-Yves Scoazec, Voichita Suciu","doi":"10.1159/000545391","DOIUrl":"10.1159/000545391","url":null,"abstract":"<p><strong>Introduction: </strong>Nowadays, there is an increasing adoption of digital pathology for diagnostic purposes.</p><p><strong>Aim: </strong>Herein we study the feasibility of cytopathological diagnosis by whole-slide imaging (WSI) in daily practice.</p><p><strong>Methods: </strong>One hundred and ten consecutive non-gynecologic cytopathology cases, originally diagnosed under light microscopy (LM) by two pathologists, were scanned at ×40. Then, cases were diagnosed on WSI, by the same pathologist who originally rendered the conventional diagnosis. The pathologists were blinded to the diagnosis made by LM, but they had access to the same clinical information. The washout period was at least 3 months. WSI diagnoses were compared to the original LM diagnoses, and cases were considered concordant if the two types of diagnosis were identical.</p><p><strong>Results: </strong>LM and the WSI diagnoses were concordant in 87.3% [95% CI: 79.6; 92.9] of cases. Intra-observer agreement was lowest for thyroid fine-needle aspiration cytology (only 1 case out of 5). After the exclusion of thyroid cases, the concordance rate between WSI and LM was 90.5% [95% CI: 83.2%; 95.3%].</p><p><strong>Conclusion: </strong>Primary cytological diagnosis can be done using our digital system.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-8"},"PeriodicalIF":1.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}