Acta Histochemica Et Cytochemica最新文献_第5页

Role of Cancer Stem-like Cells in the Process of Invasion and Mesenchymal Transformation by a Reconstituted Triple-negative Breast Cancer Cell Population Resistant to p53-induced Apoptosis. 肿瘤干细胞样细胞在抗p53诱导凋亡重组三阴性乳腺癌细胞群侵袭和间充质转化过程中的作用

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-10-28 Epub Date: 2022-10-25 DOI: 10.1267/ahc.22-00076

Sana Inoue, Miki Imanishi, Ai Kanzaki, Atsumi Fujimoto, Marina Maeyama, Ayaka Okamoto, Hiroka Matsuda, Kiyotsugu Yoshikawa, Rei Takahashi

{"title":"Role of Cancer Stem-like Cells in the Process of Invasion and Mesenchymal Transformation by a Reconstituted Triple-negative Breast Cancer Cell Population Resistant to p53-induced Apoptosis.","authors":"Sana Inoue, Miki Imanishi, Ai Kanzaki, Atsumi Fujimoto, Marina Maeyama, Ayaka Okamoto, Hiroka Matsuda, Kiyotsugu Yoshikawa, Rei Takahashi","doi":"10.1267/ahc.22-00076","DOIUrl":"https://doi.org/10.1267/ahc.22-00076","url":null,"abstract":"We investigated the role of cancer stem cells (CSCs) in a population of triple-negative breast cancer (TNBC) cells that are resistant to apoptosis. A human breast cancer cell population capable of inducing p53 expression with doxycycline (Dox) was created and used as an untreated control (UT). After the addition of Dox to UT for 5 days, the cell population reconstituted with cells showing resistance to apoptosis was named RE. Fluorescence-activated cell sorting (FACS) and immunostaining revealed that after the addition of Dox, the ratio of cells in the S and G2/M phases decreased in UT as apoptosis proceeded, but did not markedly change in apoptosis-resistant RE. CSC-like cells in RE exhibited a cell morphology with a larger ratio of the major/minor axis than UT. FACS showed that RE had a higher proportion of CSC-like cells and contained more CD44+CD24- mesenchymal CSCs than ALDH1A3+ epithelial-like CSCs. In a Matrigel invasion assay, UT was more likely to form a three-dimensional cell population, whereas RE exhibited a planar population, higher migration ability, and the up-regulated expression of epithelial-mesenchymal transition-related genes. These results provide insights into the mechanisms by which TNBC cells acquire treatment resistance at the time of recurrence.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2f/c2/ahc-055-169.PMC9631983.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40697213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Optimized Mouse-on-mouse Immunohistochemical Detection of Mouse ESR2 Proteins with PPZ0506 Monoclonal Antibody. PPZ0506单克隆抗体优化小鼠ESR2蛋白的小鼠对小鼠免疫组化检测

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-10-28 Epub Date: 2022-10-25 DOI: 10.1267/ahc.22-00043

Mina Ozawa, Yujiro Hattori, Shimpei Higo, Mai Otsuka, Keisuke Matsumoto, Hitoshi Ozawa, Hirotaka Ishii

{"title":"Optimized Mouse-on-mouse Immunohistochemical Detection of Mouse ESR2 Proteins with PPZ0506 Monoclonal Antibody.","authors":"Mina Ozawa, Yujiro Hattori, Shimpei Higo, Mai Otsuka, Keisuke Matsumoto, Hitoshi Ozawa, Hirotaka Ishii","doi":"10.1267/ahc.22-00043","DOIUrl":"https://doi.org/10.1267/ahc.22-00043","url":null,"abstract":"Despite the physiological significance of ESR2, a lack of well-validated detection systems for ESR2 proteins has hindered progress in ESR2 research. Thus, recent identification of a specific anti-human ESR2 monoclonal antibody (PPZ0506) and its specific cross-reactivity against mouse and rat ESR2 proteins heightened momenta toward development of appropriate immunohistochemical detection systems for rodent ESR2 proteins. Building upon our previous optimization of ESR2 immunohistochemical detection in rats using PPZ0506, in this study, we further aimed to optimize mouse-on-mouse immunohistochemical detection using PPZ0506. Our assessment of several staining conditions using paraffin-embedded ovary sections revealed that intense heat-induced antigen retrieval, appropriate blocking, and appropriate antibody dilutions were necessary for optimization of mouse-on-mouse immunohistochemistry. Subsequently, we applied the optimized immunostaining method to determine expression profiles of mouse ESR2 proteins in peripheral tissues and brain subregions. Our analyses revealed more localized distribution of mouse ESR2 proteins than previously assumed. Moreover, comparison of these results with those obtained in humans and rats using PPZ0506 revealed interspecies differences in ESR2 expression. We expect that our optimized methodology for immunohistochemical staining of mouse ESR2 proteins will help researchers to solve multiple lines of controversial evidence concerning ESR2 expression.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1e/6b/ahc-055-159.PMC9631985.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40698206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

An Advanced Detection System for In Situ Hybridization Using a Fluorescence Resonance Energy Transfer-based Molecular Beacon Probe. 基于荧光共振能量转移分子信标探针的原位杂交先进检测系统。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-10-28 DOI: 10.1267/ahc.22-00075

Narantsog Choijookhuu, Yasuaki Shibata, Takumi Ishizuka, Yan Xu, Takehiko Koji, Yoshitaka Hishikawa

{"title":"An Advanced Detection System for In Situ Hybridization Using a Fluorescence Resonance Energy Transfer-based Molecular Beacon Probe.","authors":"Narantsog Choijookhuu, Yasuaki Shibata, Takumi Ishizuka, Yan Xu, Takehiko Koji, Yoshitaka Hishikawa","doi":"10.1267/ahc.22-00075","DOIUrl":"https://doi.org/10.1267/ahc.22-00075","url":null,"abstract":"In situ hybridization (ISH) is a powerful method for detecting specific RNAs at the cellular level. Although conventional ISH using hapten-labeled probes are useful for detecting multiple RNAs, the detection procedures are still complex and required longer time. Therefore, we introduced a new application of fluorescence resonance energy transfer (FRET)-based molecular beacon (MB) probes for ISH. MCF-7 cells and C57BL/6J mouse uterus were used for ISH. MB probes for ERα mRNA and 28S rRNA were labeled with Cy3/BHQ-2 and 6-FAM/DABCYL, and conventional probes were labeled with digoxigenin. Fluorescence measurements revealed that of more-rapid hybridization kinetics compared to conventional probes. In MCF-7 cells, 28S rRNA was detected in nucleolus and cytoplasm of all cells, whereas ERα mRNA was detected in some nucleolus. In the uterus, 28S rRNA was clearly detected using complementary MB probe, but there were no signals in control slides. Moreover, 28S rRNA was detected in all cells, whereas ERα mRNA was detected mainly in the epithelium. Fluorescence intensity of 28S rRNA was decreased significantly in 1 or 2 base-mismatched sequences, that indicates highly specific detection of target RNAs. In conclusion, the FRET-based MB probes are very useful for ISH, providing rapid hybridization, high sensitivity and specificity.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ae/9a/ahc-055-119.PMC9631986.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9108416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Enzyme-labeled Antigen Method: Factors Influencing the Deterioration of Antigen-binding Activity of Specific Antibodies during Formalin Fixation and Paraffin Embedding. 酶标抗原法:影响福尔马林固定和石蜡包埋过程中特异性抗体抗原结合活性下降的因素。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-10-28 Epub Date: 2022-10-25 DOI: 10.1267/ahc.22-00023

Yasuyoshi Mizutani, Kazuya Shiogama, Ken-Ichi Inada, Toshiyuki Takeuchi, Atsuko Niimi, Motoshi Suzuki, Yutaka Tsutsumi

{"title":"Enzyme-labeled Antigen Method: Factors Influencing the Deterioration of Antigen-binding Activity of Specific Antibodies during Formalin Fixation and Paraffin Embedding.","authors":"Yasuyoshi Mizutani, Kazuya Shiogama, Ken-Ichi Inada, Toshiyuki Takeuchi, Atsuko Niimi, Motoshi Suzuki, Yutaka Tsutsumi","doi":"10.1267/ahc.22-00023","DOIUrl":"https://doi.org/10.1267/ahc.22-00023","url":null,"abstract":"The enzyme-labeled antigen method is an immunohistochemical technique detecting plasma cells producing specific antibodies in tissue sections. The probe is an antigen labeled with an enzyme or biotin. This immunohistochemical technique is appliable to frozen sections of paraformaldehyde (PFA)-fixed tissues, but it has been difficult to apply it to formalin-fixed, paraffin-embedded (FFPE) sections. In the current study, factors inactivating the antibody reactivity during the process of preparing FFPE sections were investigated. Lymph nodes of rats immunized with horseradish peroxidase (HRP) or a mixture of keyhole limpet hemocyanin/ovalbumin/bovine serum albumin were employed as experimental models. Plasma cells producing specific antibodies, visualized with HRP (as an antigen with enzymatic activity) or biotinylated proteins in 4% PFA-fixed frozen sections, significantly decreased in unbuffered 10% formalin-fixed frozen sections. The positive cells were further decreased by paraffin embedding following formalin fixation. In paraffin-embedded sections fixed in precipitating fixatives such as ethanol and acetone and those prepared with the AMeX method, the antigen-binding reactivity of antibodies was preserved. Fixation in periodate-lysine-paraformaldehyde and Zamboni solution also kept the antigen-binding reactivity in paraffin to some extent. In conclusion, formalin fixation and paraffin embedding were major causes inactivating antibodies. Precipitating fixatives could retain the antigen-binding reactivity of antibodies in paraffin-embedded sections.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/03/3b/ahc-055-129.PMC9631987.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40698207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effect of Ebselen on Ischemia-reperfusion Injury in Epigastric Skin Flaps in Rats. 艾布selen对大鼠上腹部皮瓣缺血再灌注损伤的保护作用。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-10-28 Epub Date: 2022-10-25 DOI: 10.1267/ahc.22-00062

Takahiko Kuroki, Susumu Takekoshi, Kanae Kitatani, Chikara Kato, Muneo Miyasaka, Tadashi Akamatsu

{"title":"Protective Effect of Ebselen on Ischemia-reperfusion Injury in Epigastric Skin Flaps in Rats.","authors":"Takahiko Kuroki, Susumu Takekoshi, Kanae Kitatani, Chikara Kato, Muneo Miyasaka, Tadashi Akamatsu","doi":"10.1267/ahc.22-00062","DOIUrl":"https://doi.org/10.1267/ahc.22-00062","url":null,"abstract":"The purpose of this study was to determine the role of oxidized diacylglycerol (DAG) and the molecular mechanism underlying ischemia-reperfusion (I/R) injury in rat skin flaps. The protective effect of ebselen on the viability of rat skin flaps with I/R injury was investigated. Flaps were designed and raised in the left inguinal region. Then, a microvascular clamp was applied to the vascular pedicle and reperfused after 6 hr. After 7 days of I/R (I/R group), the skin flap survival area ratio was significantly reduced compared to the normal skin. The administration of ebselen significantly improved the ratio compared to the I/R group. The flap survival area ratio of the I/R + ebselen group was significantly improved compared to the I/R + vehicle group. In the I/R + ebselen group, the oxidized DAG content and intensity of phosphorylated PKCα and PKCδ were significantly lower compared to the I/R + vehicle group. Furthermore, the inflammatory response was suppressed in the I/R + ebselen group compared to the I/R + vehicle group. These results indicate that ebselen is useful as a preventive and therapeutic agent for skin flap necrosis caused by I/R, because of reduction and elimination of oxidized DAG.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ce/c2/ahc-055-149.PMC9631984.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40697214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphologic Analysis of M2 Macrophage in Glioblastoma: Involvement of Macrophage Extracellular Traps (METs). 胶质母细胞瘤中 M2 巨噬细胞的形态学分析：巨噬细胞胞外陷阱 (MET) 的参与。

IF 1.6 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-08-27 Epub Date: 2022-08-10 DOI: 10.1267/ahc.22-00018

Ayano Michiba, Kazuya Shiogama, Tetsuya Tsukamoto, Masaya Hirayama, Seiji Yamada, Masato Abe

{"title":"Morphologic Analysis of M2 Macrophage in Glioblastoma: Involvement of Macrophage Extracellular Traps (METs).","authors":"Ayano Michiba, Kazuya Shiogama, Tetsuya Tsukamoto, Masaya Hirayama, Seiji Yamada, Masato Abe","doi":"10.1267/ahc.22-00018","DOIUrl":"10.1267/ahc.22-00018","url":null,"abstract":"Macrophages are classified into two phenotypes, M1 and M2, based on their roles. M2 macrophages suppress inflammation and increase in proportion to the malignancy of brain tumors. Recently, macrophage extracellular traps (METs), which change into a network, have been reported as a unique form of macrophage cell death. In this study, immunohistochemical analysis of macrophages in METs in human glioblastoma was performed. To distinguish between M1 and M2 macrophages, multiple immunostainings with Iba1 combined with CD163 or CD204 were performed. M2 macrophages were present in small amounts in normal and borderline areas but showed an increasing trend as they shifted to tumor areas, and most of them were the activated- or phagocytic-type. We also successfully detected METs coexisting with fibrin and lactoferrin near the border between the tumor and necrotic area. M2 macrophages not only suppressed inflammation but also were involved in the formation of METs. This study found that M2 macrophages play various roles in unstable situations.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/19/5c/ahc-055-111.PMC9427541.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40349780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EPLINβ Is Involved in the Assembly of Cadherin-catenin Complexes in Osteoblasts and Affects Bone Formation. EPLINβ参与成骨细胞钙粘蛋白-连环蛋白复合物的组装并影响骨形成

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-06-29 Epub Date: 2022-06-25 DOI: 10.1267/ahc.22-00027

Shihoko Miyazaki, Taro Funamoto, Tomohisa Sekimoto, Syuji Kurogi, Tomomi Ohta, Takuya Nagai, Takuya Tajima, Mai Imasaka, Kumiko Yoshinobu, Kimi Araki, Masatake Araki, Narantsog Choijookhuu, Yoshitaka Hishikawa, Etsuo Chosa

{"title":"EPLINβ Is Involved in the Assembly of Cadherin-catenin Complexes in Osteoblasts and Affects Bone Formation.","authors":"Shihoko Miyazaki, Taro Funamoto, Tomohisa Sekimoto, Syuji Kurogi, Tomomi Ohta, Takuya Nagai, Takuya Tajima, Mai Imasaka, Kumiko Yoshinobu, Kimi Araki, Masatake Araki, Narantsog Choijookhuu, Yoshitaka Hishikawa, Etsuo Chosa","doi":"10.1267/ahc.22-00027","DOIUrl":"https://doi.org/10.1267/ahc.22-00027","url":null,"abstract":"Epithelial protein lost in neoplasm (EPLIN) is an actin-associated cytoskeletal protein that plays an important role in epithelial cell adhesion. EPLIN has two isoforms: EPLINα and EPLINβ. In this study, we investigated the role of EPLINβ in osteoblasts using EPLINβ-deficient (EPLINβGT/GT ) mice. The skeletal phenotype of EPLINβGT/GT mice is indistinguishable from the wildtype (WT), but bone properties and strength were significantly decreased compared with WT littermates. Histomorphological analysis revealed altered organization of bone spicules and osteoblast cell arrangement, and decreased alkaline phosphatase activity in EPLINβGT/GT mouse bones. Transmission electron microscopy revealed wider intercellular spaces between osteoblasts in EPLINβGT/GT mice, suggesting aberrant cell adhesion. In EPLINβGT/GT osteoblasts, α- and β-catenins and F-actin were observed at the cell membrane, but OB-cadherin was localized at the perinuclear region, indicating that cadherin-catenin complexes were not formed. EPLINβ knockdown in MC3T3-e1 osteoblast cells showed similar results as in calvaria cell cultures. Bone formation markers, such as RUNX2, Osterix, ALP, and Col1a1 mRNA were reduced in EPLINβ knockdown cells, suggesting an important role for EPLINβ in osteoblast formation. In conclusion, we propose that EPLINβ is involved in the assembly of cadherin-catenin complexes in osteoblasts and affects bone formation.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e0/ca/ahc_055-99.PMC9253499.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40587490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Current Status of Whole Slide Image (WSI) Standardization in Japan. 日本全幻灯片图像(WSI)标准化现状。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-06-29 Epub Date: 2022-06-25 DOI: 10.1267/ahc.22-00009

Ichiro Mori

引用次数: 1

A Histochemical Analysis of Neurofibrillary Tangles in Olfactory Epithelium, a Study Based on an Autopsy Case of Juvenile Alzheimer's Disease. 嗅觉上皮中神经原纤维缠结的组织化学分析，基于一例青少年阿尔茨海默病的尸检研究。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-06-29 Epub Date: 2022-06-25 DOI: 10.1267/ahc.22-00048

Shino Shimizu, Ichiro Tojima, Keigo Nakamura, Hideaki Kouzaki, Takeshi Kanesaka, Norihiro Ogawa, Yoshio Hashizume, Hiroyasu Akatsu, Akira Hori, Ikuo Tooyama, Takeshi Shimizu

{"title":"A Histochemical Analysis of Neurofibrillary Tangles in Olfactory Epithelium, a Study Based on an Autopsy Case of Juvenile Alzheimer's Disease.","authors":"Shino Shimizu, Ichiro Tojima, Keigo Nakamura, Hideaki Kouzaki, Takeshi Kanesaka, Norihiro Ogawa, Yoshio Hashizume, Hiroyasu Akatsu, Akira Hori, Ikuo Tooyama, Takeshi Shimizu","doi":"10.1267/ahc.22-00048","DOIUrl":"https://doi.org/10.1267/ahc.22-00048","url":null,"abstract":"The pathological changes of Alzheimer's disease (AD) begin 10-20 years before clinical onset, and it is therefore desirable to identify effective methods for early diagnosis. The nasal mucosa is a target tissue for measuring AD-related biomarkers because the olfactory nerve is the only cranial nerve that is exposed to the external environment. We describe an autopsy case of rapidly advanced juvenile AD (JAD), focusing on the olfactory system. The formation of senile plaques, neurofibrillary tangles (NFTs), and neuropil threads was examined in the temporal cortex, hippocampus, olfactory bulb, and olfactory and respiratory epithelia in the bilateral olfactory clefts. Neurodegenerative changes in the olfactory and respiratory epithelia and the pathological deposition of amyloid β42 (Aβ42) and phosphorylated tau were also examined. As a result, senile plaques, NFTs, and neuropil threads were found in the temporal cortex, hippocampus, and olfactory bulb. NFTs were also found in the olfactory epithelium. Degenerated olfactory cells and their axons stained positive for phosphorylated tau. Supporting cells in the degenerated olfactory epithelium stained positive for Aβ42. In conclusion, pathological biomarkers of AD were expressed in the degenerated olfactory epithelium of this JAD patient. This observation suggests that nasal samples may be useful for the diagnosis of AD.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ea/d7/ahc_055-93.PMC9253500.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40587491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Pulmonary Neuroendocrine Cells and Small Cell Lung Carcinoma: Immunohistochemical Study Focusing on Mechanisms of Neuroendocrine Differentiation. 肺神经内分泌细胞与小细胞肺癌:神经内分泌分化机制的免疫组化研究。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2022-06-29 Epub Date: 2022-05-24 DOI: 10.1267/ahc.22-00031

Takaaki Ito, Shinji Kudoh, Kosuke Fujino, Mune Sanada, Yuki Tenjin, Haruki Saito, Yuko Nakaishi-Fukuchi, Hiroki Kameyama, Takaya Ichimura, Naoko Udaka, Noritaka Kudo, Akira Matsuo, Younosuke Sato

{"title":"Pulmonary Neuroendocrine Cells and Small Cell Lung Carcinoma: Immunohistochemical Study Focusing on Mechanisms of Neuroendocrine Differentiation.","authors":"Takaaki Ito, Shinji Kudoh, Kosuke Fujino, Mune Sanada, Yuki Tenjin, Haruki Saito, Yuko Nakaishi-Fukuchi, Hiroki Kameyama, Takaya Ichimura, Naoko Udaka, Noritaka Kudo, Akira Matsuo, Younosuke Sato","doi":"10.1267/ahc.22-00031","DOIUrl":"https://doi.org/10.1267/ahc.22-00031","url":null,"abstract":"Neuroendocrine (NE) differentiation has been histochemically detected in normal and cancer tissues and cells. Immunohistochemical analyses have provided a more detailed understanding of NE biology and pathology. Pulmonary NE cells are a rare lung epithelial type, and small cell carcinoma of the lung (SCLC) is a high-grade NE tumor. Pulmonary NE and SCLC cells share common mechanisms for NE differentiation. Neural or NE cell lineage-specific transcription factors, such as achaete-scute homologue 1 (Ascl1) and insulinoma-associated protein 1 (INSM1), are crucial for the development of pulmonary NE cells, and NE differentiation is influenced by the balance between Ascl1 and the suppressive neural transcription factor, hairy-enhancer of split 1, a representative target molecule of the Notch signaling pathway. In this review, we discuss the importance of Ascl1 and INSM1 in identifying pulmonary NE and SCLC cells and introduce Ascl1-related molecules detected by comparative RNA-sequence analyses. The molecular classification of SCLC based on the expression of lineage-specific transcription or co-transcription factors, including ASCL1, NEUROD1, POU2F3, and YAP1, was recently proposed. We attempted to characterize these 4 SCLC subtypes using integrated immunohistochemical studies, which will provide insights into the molecular characteristics of these subtypes and clarify the inter- and intratumor heterogeneities of SCLC.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ee/bd/ahc_055-75.PMC9253501.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40587494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2