Acta Histochemica Et Cytochemica最新文献_第3页

Erratum: Histomorphological Changes in a Rat Model of Polycystic Ovary Syndrome and the Contribution of Stevia Leaf Extract in Modulating the Ovarian Fibrosis, VEGF, and TGF-β Immunoexpressions: Comparison with Metformin [Acta Histochem. Cytochem. 55, 9-23 (2022)]. 勘误：多囊卵巢综合征大鼠模型的组织形态学变化及甜叶菊叶提取物对调节卵巢纤维化、血管内皮生长因子和 TGF-β 免疫表达的贡献：55, 9-23 (2022)].

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2023-12-28 DOI: 10.1267/ahc.21-00081E

引用次数: 0

A Single Administration of Progesterone during the Neonatal Period Shows No Structural Changes in Male Reproductive Tracts in Mice 在新生儿期单次注射孕酮不会改变小鼠雄性生殖道的结构

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2023-12-23 DOI: 10.1267/ahc.23-00052

Takuya Omotehara, Hiroki Nakata, Kenta Nagahori, Miyuki Kuramasu, Koichiro Ichimura, Masahiro Itoh

{"title":"A Single Administration of Progesterone during the Neonatal Period Shows No Structural Changes in Male Reproductive Tracts in Mice","authors":"Takuya Omotehara, Hiroki Nakata, Kenta Nagahori, Miyuki Kuramasu, Koichiro Ichimura, Masahiro Itoh","doi":"10.1267/ahc.23-00052","DOIUrl":"https://doi.org/10.1267/ahc.23-00052","url":null,"abstract":"The concentration of female-dominant steroid hormones, such as progesterone and estrogen, drops after birth in neonates. We have reported that neonatal estrogen treatment results in inflammation in the epididymis after puberty in male mice. Our recent study discovered that progesterone receptor was specifically expressed in efferent ducts just before birth in male mice. Therefore, this study aimed to reveal the impact of neonatal progesterone administration on the efferent ducts after puberty. Progesterone was subcutaneously administered to neonatal mice on their birthday in three groups: high-dose (200 mg/kg), low-dose (8 mg/kg), and control (cottonseed oil). Their testis and epididymis were collected at 12 weeks old. Semi-serial paraffin sections of these tissues were prepared and evaluated through PAS-hematoxylin staining. Efferent ducts were reconstructed into a three-dimensional structure, and their length and volume were analyzed. Spermatogenesis in the testis and epithelium of the tracts appeared normal, even in individuals administered with progesterone. There were no significant differences in the length and volume of the efferent ducts among the three groups. This study suggests that progesterone treatment in neonatal mice does not cause any structural changes in the male reproductive tracts at puberty, unlike the neonatal estrogen treatment.\u0000","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"22 5 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139027136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Localization of Sites of Osimertinib Action in Rat Intestine, Skin, and Lung by Immunohistochemistry 用免疫组化方法确定奥希替尼在大鼠肠道、皮肤和肺部的作用部位

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2023-12-23 DOI: 10.1267/ahc.23-00055

Yuta Yamamoto, Tetsuya Saita, Hiroto Kataoka, Rintaro Sogawa, Sakiko Kimura, Shinya Kimura, Chisato Shimanoe, Masashi Shin

引用次数: 0

Distribution of Contractile Structures in a Mouse and Human Lymph Node Associated with Lymph Flow 小鼠和人类淋巴结中与淋巴流动相关的收缩结构分布

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2023-12-23 DOI: 10.1267/ahc.23-00054

Yasuhisa Ishi, Takuya Omotehara, Xi Wu, Miyuki Kuramasu, Shinichi Kawata, Masahiro Itoh

{"title":"Distribution of Contractile Structures in a Mouse and Human Lymph Node Associated with Lymph Flow","authors":"Yasuhisa Ishi, Takuya Omotehara, Xi Wu, Miyuki Kuramasu, Shinichi Kawata, Masahiro Itoh","doi":"10.1267/ahc.23-00054","DOIUrl":"https://doi.org/10.1267/ahc.23-00054","url":null,"abstract":"Lymph nodes have contractile structures, but their distribution in a lymph node has been less considered in terms of facilitation of lymph flow. Axillary, inguinal, and mesenteric lymph nodes were collected from mice and human cadavers, and their sections were immunostained for alpha-smooth muscle actin (αSMA) and high molecular weight caldesmon (H-caldesmon). The αSMA-positive cells were localized in the capsule beneath the ceiling epithelium on the afferent side in both mice and humans. We found an additional layer of the αSMA-positive cells in the human lymph node, surrounding the inner layer perpendicularly. H-caldesmon was expressed only in these cells of the outer layer. In some human lymph nodes highly containing fat tissue in the medulla, the capsule disappeared on the efferent side, resulting in a disrupted sinusoidal lymph pathway. These findings suggest that human lymph nodes have additional smooth muscles in the outer region of the capsule to facilitate lymph flow. The αSMA-positive cells in the outer and inner layers of human lymph nodes probably have different functions in contraction. The presence of lipomatosis in a human lymph node will reduce its contribution to the lymph flow.\u0000","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"31 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139027329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visualization of Amyloid Oligomers in the Brain of Patients with Alzheimer’s Disease 阿尔茨海默病患者大脑中淀粉样蛋白寡聚体的可视化研究

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2023-12-20 DOI: 10.1267/ahc.23-00058

Ikuo Tooyama, Tomoko Kato, Hiroyasu Taguchi, Yusuke Kageyama, Kazuhiro Irie, Yukie Hirahara, Daijiro Yanagisawa

引用次数: 0

Cyclin D3 Colocalizes with Myogenin and p21 in Skeletal Muscle Satellite Cells during Early-Stage Functional Overload 早期功能超负荷时骨骼肌卫星细胞中的细胞周期蛋白 D3 与肌原蛋白和 p21 共定位

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2023-12-20 DOI: 10.1267/ahc.23-00041

Minenori Ishido

{"title":"Cyclin D3 Colocalizes with Myogenin and p21 in Skeletal Muscle Satellite Cells during Early-Stage Functional Overload","authors":"Minenori Ishido","doi":"10.1267/ahc.23-00041","DOIUrl":"https://doi.org/10.1267/ahc.23-00041","url":null,"abstract":"Myogenic cell differentiation is modulated by multiple regulatory factors, such as myogenin, p21, and cyclin D3 during myogenesis in vitro. It is also recognized that myogenin and p21 play important roles in regulating muscle satellite cell (SC) differentiation during overload-induced muscle hypertrophy in vivo. However, the expression patterns and functional role of cyclin D3 in the progress of muscle hypertrophy remain unclear. Thus, the present study investigated cyclin D3 expression in skeletal muscles during early-stage functional overload. Plantaris muscles were exposed to functional overload due to ablation of the gastrocnemius and soleus muscles. As a result, cyclin D3 expression was detected in the nuclei of SCs but not in myonuclei on day 1 after surgery. Cyclin D3 expression, after functional overload, gradually increased, reaching a maximum on day 7 along with myogenin expression. Moreover, in response to the functional overload, cyclin D3 was expressed simultaneously with myogenin and p21 in SC nuclei. Therefore, the present study suggests that cyclin D3 with myogenin and p21 may interactively regulate SC differentiation during early-stage functional overload.\u0000","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"10 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138745379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Insulinoma-Associated Protein 1 Expression in Pituitary Neuroendocrine Tumors 垂体神经内分泌肿瘤中胰岛素瘤相关蛋白 1 的表达分析

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2023-12-20 DOI: 10.1267/ahc.23-00033

Yu Hirokawa, Chie Inomoto, Kenichi Oyama, Shigeyuki Tahara, Robert Y. Osamura, Takayuki Shiomi, Akira Matsuno

{"title":"Analysis of Insulinoma-Associated Protein 1 Expression in Pituitary Neuroendocrine Tumors","authors":"Yu Hirokawa, Chie Inomoto, Kenichi Oyama, Shigeyuki Tahara, Robert Y. Osamura, Takayuki Shiomi, Akira Matsuno","doi":"10.1267/ahc.23-00033","DOIUrl":"https://doi.org/10.1267/ahc.23-00033","url":null,"abstract":"Insulinoma-associated protein 1 (INSM1) is a representative diagnostic marker of neuroendocrine neoplasms (NENs); however, it has not yet been used to diagnose pituitary neuroendocrine tumors (PitNETs), according to the 2022 World Health Organization (WHO) classification of pituitary tumors. This study aimed to examine the expression of INSM1 using immunohistochemistry, in the various cell lineages of PitNET classified by hormone secretion and transcription factor expression. INSM1 expression in PitNETs (different subtypes) and normal pituitary tissues was immunohistochemically assessed. The results were interpreted as scores of 0 (negative), 1 (focally positive), or 2 (frankly positive), depending on the proportion of cell staining. Twenty-eight of 35 PitNET cases (80%) showed INSM1 positivity in their nuclei. The staining in each histological subtype of PitNETs was as follows: somatotroph tumors, score 0 = 3/5, score 1 = 1/5, score 2 = 1/5; lactotroph tumors, score 0 = 2/5, score 1 = 1/5, score 2 = 2/5; thyrotroph tumors, score 2 = 5/5; corticotroph tumors: score 1 = 1/9, score 2 = 8/9; gonadotroph tumors, score 0 = 2/10, score 1 = 0/10, score 2 = 8/10; and unclassifiable tumor, score 1 = 1/1. INSM1 expression in most PitNETs was obtained, similar to that in the normal pituitary gland; thus, INSM1 may maintain the characteristics of anterior pituitary cells and pituitary tumors.\u0000","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"9 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138820871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical Analyses of Mammalian Target of Rapamycin (mTOR) Expression in Pituitary Neuroendocrine Tumors (PitNETs): mTOR as a Therapeutic Target for Functional PitNETs 垂体神经内分泌瘤 (PitNET) 中雷帕霉素哺乳动物靶标 (mTOR) 表达的免疫组化分析：mTOR 作为功能性 PitNET 的治疗靶标

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2023-12-20 DOI: 10.1267/ahc.23-00039

Ichiro Nakazato, Takayuki Shiomi, Kenichi Oyama, Akira Matsuno, Chie Inomoto, R. Yoshiyuki Osamura

{"title":"Immunohistochemical Analyses of Mammalian Target of Rapamycin (mTOR) Expression in Pituitary Neuroendocrine Tumors (PitNETs): mTOR as a Therapeutic Target for Functional PitNETs","authors":"Ichiro Nakazato, Takayuki Shiomi, Kenichi Oyama, Akira Matsuno, Chie Inomoto, R. Yoshiyuki Osamura","doi":"10.1267/ahc.23-00039","DOIUrl":"https://doi.org/10.1267/ahc.23-00039","url":null,"abstract":"Current therapeutic modalities for pituitary neuroendocrine tumors (PitNETs) include medication, surgery, and radiotherapy. Some patients have tumors that are refractory to current modalities. Therefore, novel treatment options are needed for patients with intractable diseases. Consequently, we examined the pathological data of PitNETs to study medical therapies. We retrospectively studied 120 patients with histologically diagnosed PitNETs. We used the data for the histopathological examination of hormones, such as growth hormone (GH), prolactin (PRL), adrenocorticotropic hormone, thyroid stimulating hormone, luteinizing hormone, follicle-stimulating hormone, and α-subunit, together with the immunohistochemical studies of the phospho-mammalian target of rapamycin (mTOR), cytokeratin (CAM5.2), somatostatin receptor (SSTR) type 2 and 5, Pit-1 (POU1F1/GHF-1), steroidogenic factor-1 (SF-1), and Tpit. GH-, PRL-, and SSTR5-immunopositive PitNETs had significantly higher percentage of mTOR-positivity, compared with GH-, PRL-, and SSTR5-immunonegative Pit NETs. Our results show that activation of the AKT/phosphatidylinositol-3-kinase pathway, including mTOR activation, might be related the development of PitNETs, especially GH- and PRL-producing PitNETs. Thus, mTOR is a potential target for treating functional PitNETs.\u0000","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"62 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138745697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Concentrations of Nucleotides Prevent Capillary Regression during Hindlimb Unloading by Inhibiting Oxidative Stress and Enhancing Mitochondrial Metabolism of Soleus Muscles in Rats 高浓度核苷酸通过抑制氧化应激和促进大鼠腓肠肌线粒体代谢防止后肢卸力时毛细血管退缩

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2023-12-20 DOI: 10.1267/ahc.23-00029

Ryosuke Nakanishi, Nagisa Hashimoto, Miho Takuwa, Jihao Xing, Mikiko Uemura, Badur un Nisa, Masayuki Tanaka, Takumi Hirabayashi, Minoru Tanaka, Hidemi Fujino

{"title":"High Concentrations of Nucleotides Prevent Capillary Regression during Hindlimb Unloading by Inhibiting Oxidative Stress and Enhancing Mitochondrial Metabolism of Soleus Muscles in Rats","authors":"Ryosuke Nakanishi, Nagisa Hashimoto, Miho Takuwa, Jihao Xing, Mikiko Uemura, Badur un Nisa, Masayuki Tanaka, Takumi Hirabayashi, Minoru Tanaka, Hidemi Fujino","doi":"10.1267/ahc.23-00029","DOIUrl":"https://doi.org/10.1267/ahc.23-00029","url":null,"abstract":"Prolonged inactivity in skeletal muscles decreases muscle capillary development because of an imbalance between pro- and antiangiogenic signals, mitochondrial metabolism disorders, and increased oxidative stress. Nucleotides have been shown to exert a dose-dependent effect on disuse-induced muscle atrophy. However, the dose-dependent effect on capillary regression in disused muscles remains unclear. Therefore, this study investigated the dose-dependent effect of nucleotides on capillary regression due to disuse. For this purpose, Wistar rats were divided into five groups as follows: control rats fed nucleotide-free diets (CON), hindlimb-unloaded rats fed nucleotide-free diets (HU), and hindlimb-unloaded rats fed 1.0%, 2.5%, and 5.0% nucleotide diets, (HU + 1.0% NT), (HU + 2.5% NT), and (HU + 5.0% NT), respectively. Unloading increased reactive oxygen species (ROS) production and decreased mitochondrial enzyme activity, thereby decreasing the number of muscle capillaries. In contrast, 5.0% nucleotide-containing diet prevented increases in ROS production and reductions in the expression levels of NAMPT, PGC-1α, and CPT-1b proteins. Moreover, 5.0% nucleotide-containing diet prevented mitochondrial enzyme activity (such as citrate synthase and beta-hydroxy acyl-CoA dehydrogenase activity) via NAMPT or following PGC-1α upregulation, thereby preventing capillary regression. Therefore, 5.0% nucleotide-containing diet is likely to prevent capillary regression by decreasing oxidative stress and increasing mitochondrial metabolism.\u0000","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"29 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138820698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beneficial Effect of Erythropoietin on Ameliorating Propionic Acid-Induced Autistic-Like Features in Young Rats. 促红细胞生成素对改善丙酸诱导的幼年大鼠自闭症样特征的有益作用。

IF 2.4 4区生物学

Acta Histochemica Et Cytochemica Pub Date : 2023-10-31 Epub Date: 2023-10-04 DOI: 10.1267/ahc.23-00027

Sara A Hosny, Alshaymaa M Abdelmenem, Taha Azouz, Samaa S Kamar, Asmaa M ShamsEldeen, Asmaa A El-Shafei

{"title":"Beneficial Effect of Erythropoietin on Ameliorating Propionic Acid-Induced Autistic-Like Features in Young Rats.","authors":"Sara A Hosny, Alshaymaa M Abdelmenem, Taha Azouz, Samaa S Kamar, Asmaa M ShamsEldeen, Asmaa A El-Shafei","doi":"10.1267/ahc.23-00027","DOIUrl":"10.1267/ahc.23-00027","url":null,"abstract":"Autism is a neurodevelopmental disorder that impairs communication and social interaction. This study investigated the possible beneficial effects of erythropoietin (EPO) on experimental autistic-like behaviors induced by propionic acid (PPA). Twenty-four rats were distributed into three groups: (i) control; (ii) PPA_Gp: daily injected subcutaneously with PPA for five consecutive days; PPA+EPO-Gp: injected with PPA, then received intraperitoneal injection of EPO once daily for two weeks. Behavioral changes in the rats were assessed. Specimens from the cerebellar hemispheres were subjected to histological and ultrastructure examination, immunohistochemistry for glial fibrillary acidic protein (GFAP) and calbindin-D28K, and biochemical analysis for glutathione peroxidase (GSH-Px), malondialdehyde (MDA), gamma amino-butyric acid (GABA), and serotonin. PPA-Gp showed significant behavioral impairment, with a significant depletion in GSH-px, GABA, and serotonin and a significant increase in MDA. Histological examination revealed reduced Purkinje cell count with ultrastructural degeneration, irregularly arranged nerve fibers in the molecular layer, astrogliosis, and significantly decreased calbindin-immunostaining compared to the control. EPO protected cerebellar structure, increased Purkinje cell count, improved neuronal morphology, reduced PPA-induced autistic-like features, alleviated neuronal oxidative stress, increased intercellular antioxidant levels, and suppressed inflammation. EPO provided significant protection against PPA-induced autistic features in rats, with structural preservation of Purkinje cells.","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"56 5","pages":"77-86"},"PeriodicalIF":2.4,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10644041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134648098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0