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Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry最新文献

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Production of active human carbonic anhydrase II in E. coli. 大肠杆菌中活性人碳酸酐酶II的产生。
Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry Pub Date : 1988-05-01 DOI: 10.3891/acta.chem.scand.42b-0314
C Forsman, G Behravan, A Osterman, B H Jonsson
{"title":"Production of active human carbonic anhydrase II in E. coli.","authors":"C Forsman,&nbsp;G Behravan,&nbsp;A Osterman,&nbsp;B H Jonsson","doi":"10.3891/acta.chem.scand.42b-0314","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.42b-0314","url":null,"abstract":"<p><p>cDNA encoding human carbonic anhydrase II has been isolated and its nucleotide sequence determined. Expression of the isolated carbonic anhydrase gene in Escherichia coli from a plasmid containing the tac promoter yielded an active enzyme at a level of about 1% of total protein.</p>","PeriodicalId":6886,"journal":{"name":"Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry","volume":"42 5","pages":"314-8"},"PeriodicalIF":0.0,"publicationDate":"1988-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13987831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 72
Regulation of the level of dolichyl phosphate in human hepatomas. 人肝癌中磷酸多辛基水平的调控。
Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry Pub Date : 1988-04-01 DOI: 10.3891/acta.chem.scand.42b-0247
I Eggens
{"title":"Regulation of the level of dolichyl phosphate in human hepatomas.","authors":"I Eggens","doi":"10.3891/acta.chem.scand.42b-0247","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.42b-0247","url":null,"abstract":"","PeriodicalId":6886,"journal":{"name":"Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry","volume":"42 4","pages":"247-9"},"PeriodicalIF":0.0,"publicationDate":"1988-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13979322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Purification of rabbit skeletal muscle troponin C. 兔骨骼肌肌钙蛋白C的纯化。
Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry Pub Date : 1988-04-01 DOI: 10.3891/acta.chem.scand.42b-0211
E Thulin, H J Vogel
{"title":"Purification of rabbit skeletal muscle troponin C.","authors":"E Thulin,&nbsp;H J Vogel","doi":"10.3891/acta.chem.scand.42b-0211","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.42b-0211","url":null,"abstract":"<p><p>Troponin C binds to phenyl-Sepharose in the presence of Ca2+ and can be eluted with EDTA. This property was used as an essential step in the purification of this protein from rabbit skeletal muscle. Troponin C was extracted with 6M urea from extensively washed ground muscle. The protein was bound to and eluted from DEAE-Sephadex, fractionated by size on Sephadex G75, and in a final step purified from UV-absorbing non-protein impurities on phenyl-Sepharose. The total yield of electrophoretically pure protein was 60 mg per 100 g of muscle, which is considerably higher than that previously obtained.</p>","PeriodicalId":6886,"journal":{"name":"Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry","volume":"42 4","pages":"211-5"},"PeriodicalIF":0.0,"publicationDate":"1988-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14533412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
The terminal reactions in polyisoprenoid biosynthesis. 聚类异戊二烯生物合成中的末端反应。
Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry Pub Date : 1988-03-01 DOI: 10.3891/acta.chem.scand.42b-0202
J Ericsson, T Ekström, T Chojnacki, G Dallner
{"title":"The terminal reactions in polyisoprenoid biosynthesis.","authors":"J Ericsson,&nbsp;T Ekström,&nbsp;T Chojnacki,&nbsp;G Dallner","doi":"10.3891/acta.chem.scand.42b-0202","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.42b-0202","url":null,"abstract":"","PeriodicalId":6886,"journal":{"name":"Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry","volume":"42 3","pages":"202-5"},"PeriodicalIF":0.0,"publicationDate":"1988-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14526206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Inhibition of the mitochondrial F1-ATPase by rose bengal mediated photooxidation. Interaction of the Fe2+ chelate of bathophenanthroline with the sensitizer. 玫瑰红介导的光氧化对线粒体f1 - atp酶的抑制作用。二邻菲罗啉的Fe2+螯合物与增敏剂的相互作用。
Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry Pub Date : 1988-03-01 DOI: 10.3891/acta.chem.scand.42b-0175
E Glaser, E Cadenas, S Andell, L Ernster
{"title":"Inhibition of the mitochondrial F1-ATPase by rose bengal mediated photooxidation. Interaction of the Fe2+ chelate of bathophenanthroline with the sensitizer.","authors":"E Glaser,&nbsp;E Cadenas,&nbsp;S Andell,&nbsp;L Ernster","doi":"10.3891/acta.chem.scand.42b-0175","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.42b-0175","url":null,"abstract":"<p><p>Rose Bengal mediated photooxidation of mitochondrial F1-ATPase and its beta-subunit resulted in inactivation and loss of about 50 and 60% of their histidine residues, respectively. The beta-subunit was not cleaved upon photooxidation. Photooxidation of histidine probably results in changes in the conformational stability of F1-ATPase leading to its inactivation. The participation of singlet molecular oxygen during the photooxidation process is suggested by the selective loss of histidine residues, while other amino acids, also sensitive to singlet oxygen attack, were not affected. Photochemical damage of F1-ATPase was prevented by various phenanthroline compounds, the order of efficiency being bathophenanthroline-Fe chelate greater than bathophenanthroline greater than orthophenanthroline-Fe chelate greater than bathophenanthroline-sulfonate-Fe chelate. The prevention by bathophenanthroline-Fe chelate of photochemical damage is interpreted on the basis of its interaction with the photosensitizer, Rose Bengal, probably implying a chemical reaction which decreases the actual concentration of the sensitizer and, thereby, the extent of photoinactivation.</p>","PeriodicalId":6886,"journal":{"name":"Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry","volume":"42 3","pages":"175-82"},"PeriodicalIF":0.0,"publicationDate":"1988-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14036344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Cell cycle regulation of deoxyribonucleoside kinase activities in cultured mouse fibroblasts. 培养小鼠成纤维细胞中脱氧核糖核苷激酶活性的细胞周期调控。
Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry Pub Date : 1988-02-01 DOI: 10.3891/acta.chem.scand.42b-0122
A Habteyesus, S Eriksson
{"title":"Cell cycle regulation of deoxyribonucleoside kinase activities in cultured mouse fibroblasts.","authors":"A Habteyesus,&nbsp;S Eriksson","doi":"10.3891/acta.chem.scand.42b-0122","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.42b-0122","url":null,"abstract":"","PeriodicalId":6886,"journal":{"name":"Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry","volume":"42 2","pages":"122-4"},"PeriodicalIF":0.0,"publicationDate":"1988-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13975266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
7-Methylguanine nucleotides and their structural analogues; protolytic equilibria, complexing with magnesium (II) ion and kinetics for alkaline opening of the imidazole ring. 7-甲基鸟嘌呤核苷酸及其结构类似物;原解平衡、与镁离子络合及咪唑环碱性开环动力学。
Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry Pub Date : 1988-02-01 DOI: 10.3891/acta.chem.scand.42b-0086
E Darzynkiewicz, I Labadi, D Haber, K Burger, H Lönnberg
{"title":"7-Methylguanine nucleotides and their structural analogues; protolytic equilibria, complexing with magnesium (II) ion and kinetics for alkaline opening of the imidazole ring.","authors":"E Darzynkiewicz,&nbsp;I Labadi,&nbsp;D Haber,&nbsp;K Burger,&nbsp;H Lönnberg","doi":"10.3891/acta.chem.scand.42b-0086","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.42b-0086","url":null,"abstract":"<p><p>First-order rate constants for the alkaline opening of the imidazole ring of several 7-methylguanine nucleotides and their structural analogues were determined. The results obtained suggested that intramolecular interaction between the negatively charged 5'-phosphate group and the positively charged imidazole ring markedly retard the attack of hydroxide ion on the C8 atom of the 7-methylguanine ring. In contrast, hardly any influence on the acidities of the interacting base and phosphate moieties was detectable. No effect on the complexing of the phosphate group with magnesium(II) ion could be detected.</p>","PeriodicalId":6886,"journal":{"name":"Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry","volume":"42 2","pages":"86-92"},"PeriodicalIF":0.0,"publicationDate":"1988-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14503700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Studies on fatty acid elongation and desaturation in rat liver phospholipids in vivo. 大鼠肝脏磷脂体内脂肪酸延伸和去饱和的研究。
Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry Pub Date : 1988-02-01 DOI: 10.3891/acta.chem.scand.42b-0130
A Jakobsson, J Ericsson, G Dallner
{"title":"Studies on fatty acid elongation and desaturation in rat liver phospholipids in vivo.","authors":"A Jakobsson,&nbsp;J Ericsson,&nbsp;G Dallner","doi":"10.3891/acta.chem.scand.42b-0130","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.42b-0130","url":null,"abstract":"","PeriodicalId":6886,"journal":{"name":"Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry","volume":"42 2","pages":"130-2"},"PeriodicalIF":0.0,"publicationDate":"1988-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14503699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Quantitative analysis of emepronium bromide by demethylation and selected ion monitoring GC/MS. 去甲基化和选择离子监测气相色谱/质谱法定量分析溴化安咪铵。
Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry Pub Date : 1988-01-01 DOI: 10.3891/acta.chem.scand.42b-0059
L Kenne, B Norén, S Strömberg
{"title":"Quantitative analysis of emepronium bromide by demethylation and selected ion monitoring GC/MS.","authors":"L Kenne,&nbsp;B Norén,&nbsp;S Strömberg","doi":"10.3891/acta.chem.scand.42b-0059","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.42b-0059","url":null,"abstract":"","PeriodicalId":6886,"journal":{"name":"Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry","volume":"42 1","pages":"59-61"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14490034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Endogenous synthesis of taurine and GABA in rat ocular tissues. 大鼠眼组织中牛磺酸和GABA的内源性合成。
Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry Pub Date : 1988-01-01 DOI: 10.3891/acta.chem.scand.42b-0039
A A Heinämäki
{"title":"Endogenous synthesis of taurine and GABA in rat ocular tissues.","authors":"A A Heinämäki","doi":"10.3891/acta.chem.scand.42b-0039","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.42b-0039","url":null,"abstract":"<p><p>The endogenous production of taurine and gamma-aminobutyric acid (GABA) in rat ocular tissues was investigated. The activities of taurine-producing enzyme, cysteine sulfinic acid decarboxylase (CSAD), and GABA-synthesizing enzyme, glutamic acid decarboxylase (GAD), were observed in the retina, lens, iris-ciliary body and cornea. The highest specific activity of CSAD was in the cornea and that of GAD in the retina. The discrepancy between CSAD activity and taurine content within the ocular tissues indicates that intra- or extraocular transport processes may regulate the concentration of taurine in the rat eye. The GAD activity and the content of GABA were distributed in parallel within the rat ocular tissues. The quantitative results suggest that the GAD/GABA system has functional significance only in the retina of the rat eye.</p>","PeriodicalId":6886,"journal":{"name":"Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry","volume":"42 1","pages":"39-42"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14490031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
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