{"title":"大肠杆菌中活性人碳酸酐酶II的产生。","authors":"C Forsman, G Behravan, A Osterman, B H Jonsson","doi":"10.3891/acta.chem.scand.42b-0314","DOIUrl":null,"url":null,"abstract":"<p><p>cDNA encoding human carbonic anhydrase II has been isolated and its nucleotide sequence determined. Expression of the isolated carbonic anhydrase gene in Escherichia coli from a plasmid containing the tac promoter yielded an active enzyme at a level of about 1% of total protein.</p>","PeriodicalId":6886,"journal":{"name":"Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry","volume":"42 5","pages":"314-8"},"PeriodicalIF":0.0000,"publicationDate":"1988-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"72","resultStr":"{\"title\":\"Production of active human carbonic anhydrase II in E. coli.\",\"authors\":\"C Forsman, G Behravan, A Osterman, B H Jonsson\",\"doi\":\"10.3891/acta.chem.scand.42b-0314\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>cDNA encoding human carbonic anhydrase II has been isolated and its nucleotide sequence determined. Expression of the isolated carbonic anhydrase gene in Escherichia coli from a plasmid containing the tac promoter yielded an active enzyme at a level of about 1% of total protein.</p>\",\"PeriodicalId\":6886,\"journal\":{\"name\":\"Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry\",\"volume\":\"42 5\",\"pages\":\"314-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"72\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3891/acta.chem.scand.42b-0314\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3891/acta.chem.scand.42b-0314","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Production of active human carbonic anhydrase II in E. coli.
cDNA encoding human carbonic anhydrase II has been isolated and its nucleotide sequence determined. Expression of the isolated carbonic anhydrase gene in Escherichia coli from a plasmid containing the tac promoter yielded an active enzyme at a level of about 1% of total protein.
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