中华临床医师杂志(电子版)最新文献

{"title":"Prevention of tumor recurrence with Sorafenib after liver transplantation in rat with liver cancer","authors":"Yang Zhenlin, Zou Weiwei","doi":"10.3877/279","DOIUrl":"https://doi.org/10.3877/279","url":null,"abstract":"Objective To investigate the prevention and treatment of tumor recurrence with Sorafenib after orthotopic liver transplantation(OLT)for primary carcinoma of liver in rats.Methods Portal vein injection of Walker-256 cancer cells to establish models of metastasis and recurrence of liver after liver transplantation in rats.The rats were randomly divided into 4 groups(n=16):Control group(group A);Sorafenib low-dose group(group B);Sorafenib medium-dose group(group C);Sorafenib high-dose group(group D);moreover,a normal group(group E,n=8).Eight rats of each group were chosen 20 days after operation to have observation of pathological changes in liver,which were made with hematoxylin-eosin sectioning(HE);vascular endothelial growth factor(VEGF)and microvessel density(MVD)level of liver and lung tissues were detected by immunohistochemistry;bivariate correlation analysis of VEGF and MVD of liver were used to identify the relationship between them;survival time of remaining 8 rats from control group and experimental groups of Sorafenib were observed.Results There were no differences in tumor metastasis and recurrence among control group and Sorafenib experimental groups,for it could be found metastasis and recurrence in rat liver of each group,no cancer metastasis or recurrence found in lung of each group,however,Sorafenib experimental groups had signifcantly higher ratio of cancer cell apoptosis,lower ratio of necrosis of liver cells compared with control group;VEGF-positive ratio and MVD counts of liver in each experimental group were smaller than that of control group(P0.01);VEGF and MVD in liver were highly correlated(P0.01),correlation coefficient rp=0.84;there was no difference of VEGF and MVD in lung tissue of each group(P0.05);survival rate of medium-dose group and high-dose group were higher(P0.05).Conclusions Sorafenib could inhibit synthesis of VEGF in tumor tissue,which could inhibit angiogenesis of tumor,then tumor growth,as well as invasion to normal liver cells were inhibited,and liver function damage was slighter,survival time were prolonged.As a result,there was a positive therapeutic effect of application of Sorafenib after orthotopic liver transplantation(OLT)for primary carcinoma of liver in rats.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"5 1","pages":"335-342"},"PeriodicalIF":0.0,"publicationDate":"2011-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70277968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive value of clinical and echocardiographic parameters on cardiac resynchronization therapy","authors":"Gongao Xue, Su Yangang, Pan Wen-zhi, C. Jie, Pan Cuizhen, Wang Wei, B. Jin, Qin Shengmei, G. Jun-bo, Shu Xian-hong","doi":"10.3877/283","DOIUrl":"https://doi.org/10.3877/283","url":null,"abstract":"Objective　To assess the value of echocardiographic parameters， N-terminal pro-brain natriuretic peptide (NT-proBNP)， and the duration of QRS wave (QRSd) in predicting the response to cardiac resynchronization therapy (CRT).Methods　Eighty patients accepted CRT because of refractory heart failure were enrolled. Echocardiography was performed to acquire two-dimensional echocardiography parameters and to evaluate inter-and intra-ventricular dyssynchrony before and after 6 months of CRT. The concentration of NT-proBNP was detected and QRSd was measured before and after 7 days of CRT implantation. At least 15% reduction in LVESV at the 6-month after CRT was defined as responders. Results　The standard deviation of time to peak myocardial systolic velocity of all 12 LV segments(Ts-12SD) is relatively valuable in predicting CRT responders (AUC＝0.703， P ＝0.019； Ts-12SD≥34.6 ms has the relatively high sensitivity of 61.8% and specificity of 70.6%)， other synchrony parameters could not predict the response to CRT(NS). The level of NT-proBNP before CRT implantation could predict the response to CRT (AUC＝0.75，P＝0.005). The QRSd could not predict the response to CRT(NS).Conclusions　Only Ts-12SD of echocardiographic parameters which were usually used could predict the response to CRT. The level of NT-proBNP before CRT implantation is valuable in predicting the response to CRT. The QRSd could not predict the response to CRT.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"36 1","pages":"368-372"},"PeriodicalIF":0.0,"publicationDate":"2011-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70277985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of histopathological changes in testicular biopsy of men with azospermia in Zhejiang and Shandong province","authors":"Chen Hongmei, Huang Cai-er, Zong Chuan-zhen, S. Yan, Cao Rui-xue, Xia Chengqing","doi":"10.3877/292","DOIUrl":"https://doi.org/10.3877/292","url":null,"abstract":"Objective To investigate and compare the pathological causes of azospermia in Shandong and Zhejiang provinces and that in different range of ages in China.Methods Testicular biopsies of 95 men with azospermia from Zhejiang province and 202 from Shandong province were reevaluated retrospectively.Results Of the 297 cases,28.61% were classified as obstructive azospermia,8.41% were hypospermatogenesis,32.65% were spermatocytic arrest,20.53% were sertoli cell only,9.76% were tubular fibrosis.The rates of obstructive azospermia in Zhejiang and Shandong province were 36.84% and 24.75% respectively,and the difference reached statistic significant(P=0.04).The rates of obstructive azospermia in men below the age of 30 and men in age of 30 and above were 24.52% and 33.82% respectively,and the rates of spermatocytic arrest of the two age groups were 38.82% and 21.17% respectively,both the differences reached statistic significant(P=0.02,P=0.01).One hundred and thirty-three cases with bilateral testicular biopsy showed similar morphological changes in both testes.Conclusions The constitutions of pathological causes of azospermia in different parts of China and in different age groups were different from each other.Histopathology changes in both testes were similar to each other in men with azospermia.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"5 1","pages":"423-426"},"PeriodicalIF":0.0,"publicationDate":"2011-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70278097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and clinical significances of TGF-β1 and MMP-2 in papillary thyroid carcinoma with multiple foci","authors":"Yuan Zong-huai, Cheng Xi-ping, Zhang Ai-li, Wang Gang-ping","doi":"10.3877/301","DOIUrl":"https://doi.org/10.3877/301","url":null,"abstract":"Objective　To study the expression and clinical significance of TGF-β1 and MMP-2 in mulitifocal papillary thyroid carcinoma (PTC).Methods　Immunohistochemical S-P method was used to investigate the expression of TGF-β1 and MMP-2 in 47 cases of mulitifocal PTC and adjacent tissues.The expression of TGF-β1 and MMP-2 and the risk factors were analyzed between solitary PTC and multifocal PTC group.Results　There were significant difference between mulitifocal thyroid cancer tissues,single focal PTCs and the adjacent tissue (P<0.05).Overexpressions of TGF-β1 and MMP-2 in mulitifocal PTCs were related to the degree of the infiltration,lymph node metastasis and tumor recurrence(P<0.05).The expression of TGF-β1 was correlated positively with MMP-2 (r＝0.920,P＝0.000).There was significant difference in expressions of TGF-β1 and MMP-2 between mulitifocal and single focal PTCs in lymph node metastasis and tumor recurrence groups (P<0.05).Conclusions　The high expressions of TGF-β1 and MMP-2 in mulitifocal PTCs relate to the development,invasion,metastasis and recurrence.TGF-β1 and MMP-2 might be considered as biologic behavior indicators of mulitifocal PTC.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"5 1","pages":"691-695"},"PeriodicalIF":0.0,"publicationDate":"2011-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70278134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perioperation outcome of hypertension and renal function after renal arterial revascularization for atherosclerotic renal artery stenosis","authors":"Z. You, Chen Bin, Heng-xi Yu, Jian‐xin Li, Yongquan Gu, Yi-xia Qi, Luo Tao, G. Lianrui, Yang Sheng-jia, Z. Jian","doi":"10.3877/800","DOIUrl":"https://doi.org/10.3877/800","url":null,"abstract":"Objective To explore the effect of the surgical treatment for preoperative period outcome of hypertension and renal function in atherosclerotic renal artery stenosis(ARAS) patients.Methods 80 patients with 108 stenotic renal arteries in total between August 2006 and May 2010 were retrospectively analyzed.The sex ratio(M/F) was 1.8∶1 and average age was (64.8±11.1) years old.The renal artery stenosis was confirmed by renal artery angiography.Fifty-two patients suffered unilateral ARAS and 28 patients suffered bilateral ARAS,including 20 mild-mid range stenotic renal arteries,82 severe ones and 6 occlusive ones.Renal artery stenting treatment were performed in 67 patients,angioplasty treatment in 3 and 3 underwent traditional surgical operation.The other 7 patients were treated by medication.Results All the surgical treatments were performed successfully with 38.4% cases renal function improvement and 73.9% cases improved for hypertension.The post-operative blood pressures were improved with a statistical significance(P=0.000).Meanwhile,there were no significant differences for blood creatinine,blood urea nitrogen,glomerular filtration rate (GFR) before and after the operation.A significant difference was found between bilateral and unilateral ARAS patients for their GRFs improvement(P=0.038).Conclusions The surgical treatment can effectively help to relieve the conditions of hypertension from ARAS.For the reservation of renal functions,the bilateral ARAS patients undergoing oneside treatment seemed to benefit more from the operation than unilateral ARAS patients.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"5 1","pages":"7196-7201"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70279561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of NMO-IgG or anti-AQP4 antibodies:a specifity challenge","authors":"Sun Qiao-song, Liu Jun-xiu, Feng Yan-qing, Fang Yi-min, G. Ning, L. Rong, Chen Xi, H. Fan","doi":"10.3877/195","DOIUrl":"https://doi.org/10.3877/195","url":null,"abstract":"Objective Antibody against aquaporin-4 ( AQP4) is a validated specific biomarker for the neuromyelitis optica ( NMO) spectrum disorders. However,the specifity of NMO-IgG/anti-AQP4 has not been systemically tested in CNS infectious diseases. This study was undertaken to investigate whether anti-AQP4 antibody is present in patients with tuberculous meningitis ( TBM) and to compare different assays for assessing the seroprevalence of anti-AQP4 antibodies in patients with TBM and NMO spectrum disorders. Methods We set up the conventional NMO-IgG assay,based on indirect immunofluorescent ( IIF) reactivity with mouse brain cryosections,and an AQP4-transfected cell-based assay ( CBA). IIF we characterized further by dual immunostaining,and the antibody titers were also analyzed. Both assays were used in parallel to test seropositivity of masked serum samples from patients with neuromyelitis optica ( NMO,24) ,longitudinally extensive transverse myelitis ( LETM,22) ,multiple sclerosis ( MS,30) ,pulmonary tuberculosis ( PTB,30 ) ,tuberculosis meningitis ( TBM,46 ) and healthy controls ( HC,20 ). Results Serum NMO-IgG/anti-AQP4 antibody was not exclusively restricted to patients with NMO spectrum disorders. The antibody was detected in the majority of serum samples from TBM patients,and titers ofanti-AQP4 IgG in TBM patients were higher than that in those with confirmed NMO and LTEM. Using the IIF assay,seropositivity rates for NMO-IgG were 76. 1% ( 35 /46) for TBM patients,62. 5% ( 15 /24) for NMO patients,and 59. 1% ( 13 /22) for LETM. Using cell-based assays we found antibodies to AQP4 in 91. 3% ( 42 /46) of TBM patients,91. 7% ( 22 /24) for NMO patients,and 86. 4% ( 19 /22) for LETM. PTB,MS,and HC all had low rates of anti-AQP4 IgG seropositivity in these assays. Conclusions The data we present suggests that aquaporin-4 autoimmunity may reflect a CNS mycobacteria-initiated immune response. This findings challenge the classic view that NMO-IgG is a disease-specific antibody,and suggests NMO-IgG/anti-AQP4 may be a marker of destructive demyelination.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"4 1","pages":"2147-2152"},"PeriodicalIF":0.0,"publicationDate":"2010-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70277749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenic analysis and treatment of biliary tract complication following orthotopic liver transplantation","authors":"Z. Ke, Jiang-jin Li, M. Yi, Wang Feng-shui, Lu Yan, He Rong, Ding Zhen-hao, M. Yu","doi":"10.3877/186","DOIUrl":"https://doi.org/10.3877/186","url":null,"abstract":"Objective To investigate the pathogenesis of biliary tract complication following orthotopic liver transplantation ( OLT) and explore the methods of countermeasures. Methods Clinical data of 74 cases of biliary tract complication following OLT at our institute from 2004 to 2009 were retrospectively analysed. The patterns and high risk factors of biliary tract complication following OLT were summarized. Furthermore the interventing time and efficacy of any treatment for biliary tract complication following OLT were carefully analyzed. Results Bile leakage,biliary sludge,biliary duct stoma stricture,biliary ischemia stricture and biliary cast syndrome were predominant patterns of biliary tract complication following OLT. The inexperience in organ procurement,organ preservation,operating technique and post-operating treating was major factors for bile leakage and biliary duct stoma stricture. The prolong of warm ischemia time was the major factor for biliary sludge and biliary ischemia stricture ( P 0. 01) . At the same time the T-tube and cholangiojejunostomy could induce high morbility of biliary ischemia stricture( P 0. 01) . The organs to be perfused with UW through abdominal aorta had higher morbilities in biliary sludge,biliary ischemia stricture and biliary cast syndrome than those no use with UW( P 0. 05) . Endoscopic and radiative interventionswere effective methods for bile leakage,biliary sludge and biliary duct stoma stricture but unsatisfactory for treating biliary ischemia stricture and biliary cast syndrome. Retransplantation was a salvage method for invalid cases. Conclusions The biliary tract complication following OLT was directly associated with operation technique, ischemical reperfusion injury, the pattern of biliary duct anastomosis, organ procurement and so on. It was very important to think highly of preventing biliary ischemia stricture and biliary cast syndrome in order to cut down the morbilitis for those severe biliary tract complication following OLT.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"4 1","pages":"2104-2108"},"PeriodicalIF":0.0,"publicationDate":"2010-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70277741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential expression of HSF1 mRNA and HSPs in mucous membrane，adenoma and carcinoma of colon","authors":"Meng Pan-qing","doi":"10.3877/155","DOIUrl":"https://doi.org/10.3877/155","url":null,"abstract":"Objective　To detect the differential expression of HSF1 mRNA，HSP27，HSP70 and HSP90α in mucous membrane，adenoma and carcinoma of colon.Methods　By means of tissue microarray technology，detect the expression of heat shock related nucleic acid and proteins with immunohistochemistry and hybridization in situ.Results　The positive expression of HSF1 mRNA，HSP27，HSP70 and HSP90α in mucous membrane，adenoma and carcinoma of colon were 21.43%,66.00%,85.71%；35.70%,68.00%,42.90%；28.57%,46.00%,67.35%；21.40%,54.00%,73.50%.The expression level of HSF1 mRNA and HSP90α in adenoma and carcinoma of colon was obviously higher than that in mucous membrane，meanwhile the expression level of HSP27 in adenoma was higher than that in mucous membrane and carcinoma of colon，and the expression level of HSP70 in carcinomar of colon was higher than that in mucous membrane and adenoma.Conclusions　The positive expression of HSF1 mRNA，HSP27，HSP70 and HSP90α is interrelated due to the process of colon tumorigenesis，in which the heat shock stress signal pathway that represent played an important role.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"4 1","pages":"1549-1554"},"PeriodicalIF":0.0,"publicationDate":"2010-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70277964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of trichosanthin on herpes simplex virus-1 DNA polyase and DNA combine protein mRNA expression in vivo and in vitro","authors":"Chen Guang-fu, Yin Fei, Zhang Hong-yuan, Hu Wen-ge, Chen Feng-ying","doi":"10.3877/148","DOIUrl":"https://doi.org/10.3877/148","url":null,"abstract":"Objective To evaluate the effects of Trichosanthin(TCS) on Herpes simplex virus-1 (HSV-1) DNA polyase and DNA combine protein mRNA expression in vivo and in vitro.Methods Thirtysix mice were randomly assigned into three groups:Norm control group(n=12) ;Model control group(model group,n=12) ;TCS-treated group(treated group,n=12).Viral encephalitis was induced by intracranial inoculation of HSV-1 in the two groups.The TCS-treated group was injected with TCS 30 min before HSV-1 inoculation.The HSV-1 DNA polyase and DNA combine protein mRNA expression were measured at 12 h,24 h,48 h,96 h after HSV-1 inoculation,respectively.The culture neuron in vitro were assigned into four groups:Norm control group;drug control group;HSV-1 control group;TCS-pretreated group.The HSV-1 DNA polyase and DNA combine protein mRNA expression were measured at 12 h,24 h,48 h,96 h after HSV-1 were inoculated into the neuron,respectively.Results The HSV-1 DNA polyase mRNA expression of brain tissue at 12 h,24 h,48 h,96 h after HSV-1 intracranial inoculation were markedly lower in the TCS-treated group(8.39 ± 0.84,9.62 ± 0.87,7.81 ± 0.90,7.66 ± 0.85) compared with the Model group(11.26 ± 1.02,12.43 ± 1.17,11.56 ± 1.68,12.78 ± 1.71),respectively(P 0.05).The HSV-1 DNA combine protein mRNA expression of brain tissue at 12 h,24 h,48 h,96 h after HSV-1 intracranial inoculation were markedly lower in the TCS-treated group(7.28 ± 1.07,8.25 ± 0.78,7.56 ± 0.96,7.85 ± 1.22) compared with the Model group(10.45 ± 2.72,12.33 ± 1.97,11.24 ± 1.86,12.54 ± 2.32),respectively(P 0.01).The HSV-1 DNA polyase mRNA expression of neuron at 12 h,24 h,48 h,96 h after HSV-1 inoculation in the TCS-pretreated group(9.33 ± 0.87,9.57 ± 0.97,9.73 ± 0.94,9.84 ± 0.86) were markedly lower than that in the HSV-1 control group(10.15 ± 1.12,15.63 ± 1.24,12.77 ± 2.38,16.58 ± 1.92),respectively (P 0.05).The HSV-1 DNA combine protein mRNA expression of neuron at 24 h,48 h,96 h after HSV-1 inoculation in the TCS-pretreated group(7.58 ± 1.75,8.63 ± 1.79,7.38 ± 1.47) were markedly lower than that in the HSV-1 control group(10.24 ± 2.16,10.85 ± 1.67,11.63 ± 2.05),respectively(P 0.05).Conclusions TCS has inhibitive effects on the HSV-1 DNA polyase and DNA combine protein mRNA expression in vivo and in vitro.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"4 1","pages":"1520-1524"},"PeriodicalIF":0.0,"publicationDate":"2010-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70277786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the resistant genes of metal β-lactamase(MBLs) of the multi-drug resistant mechanism of Pseudomonas aeruginosa","authors":"Huang Yi-shan, Long Yan, Fangping Li, Zhu Xing-chun, Liu Qing-song, T. Zhong, Su Zhong-long","doi":"10.3877/142","DOIUrl":"https://doi.org/10.3877/142","url":null,"abstract":"Objective　To survey the distribution of aminoglycoside-modifying enzyme genes in multi-drug-resistant Pseudomonas aeruginosa(MRPA) isolated in our hospital and provide epidemiologic data for clinic. Methods　Eighteen strains of Pseudomonas aeruginosa were collected randomly from 2007 to 2009 in our hospital. Isolates were further evaluated for resistance to 14 kinds of anti-bateria mainly by VITEK-32 instrument and some by K-B method as a supplement. Three-dimensional extract test were used to screen the phenotype of MBL production. Five genes， including blaIMP， blaVIM， blaSPM， blaGIM and blaSIM， were amplified by polymerase chain reaction (PCR). Results　Of the eighteen PA， nine was resistant to imipenem. Three-dimensional extract test showed that five were positive for MBLs. The PCR results showed that four were positive for blaVIM， one was positive for blaGIM， and one was positive both for blaVIM and blaGIM. Conclusions　MBLs production was the majior mechanism for MRPA resistant to imipenen in our hospial and the genes of MBLs is of diversity.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"4 1","pages":"1623-1627"},"PeriodicalIF":0.0,"publicationDate":"2010-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70277632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}