Differential expression of HSF1 mRNA and HSPs in mucous membrane，adenoma and carcinoma of colon

Abstract

Objective　To detect the differential expression of HSF1 mRNA，HSP27，HSP70 and HSP90α in mucous membrane，adenoma and carcinoma of colon.Methods　By means of tissue microarray technology，detect the expression of heat shock related nucleic acid and proteins with immunohistochemistry and hybridization in situ.Results　The positive expression of HSF1 mRNA，HSP27，HSP70 and HSP90α in mucous membrane，adenoma and carcinoma of colon were 21.43%,66.00%,85.71%；35.70%,68.00%,42.90%；28.57%,46.00%,67.35%；21.40%,54.00%,73.50%.The expression level of HSF1 mRNA and HSP90α in adenoma and carcinoma of colon was obviously higher than that in mucous membrane，meanwhile the expression level of HSP27 in adenoma was higher than that in mucous membrane and carcinoma of colon，and the expression level of HSP70 in carcinomar of colon was higher than that in mucous membrane and adenoma.Conclusions　The positive expression of HSF1 mRNA，HSP27，HSP70 and HSP90α is interrelated due to the process of colon tumorigenesis，in which the heat shock stress signal pathway that represent played an important role.