Study on the resistant genes of metal β-lactamase(MBLs) of the multi-drug resistant mechanism of Pseudomonas aeruginosa

中华临床医师杂志(电子版) Pub Date : 2010-10-28 DOI:10.3877/142

Huang Yi-shan, Long Yan, Fangping Li, Zhu Xing-chun, Liu Qing-song, T. Zhong, Su Zhong-long

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引用次数: 1

Abstract

Objective　To survey the distribution of aminoglycoside-modifying enzyme genes in multi-drug-resistant Pseudomonas aeruginosa(MRPA) isolated in our hospital and provide epidemiologic data for clinic. Methods　Eighteen strains of Pseudomonas aeruginosa were collected randomly from 2007 to 2009 in our hospital. Isolates were further evaluated for resistance to 14 kinds of anti-bateria mainly by VITEK-32 instrument and some by K-B method as a supplement. Three-dimensional extract test were used to screen the phenotype of MBL production. Five genes， including blaIMP， blaVIM， blaSPM， blaGIM and blaSIM， were amplified by polymerase chain reaction (PCR). Results　Of the eighteen PA， nine was resistant to imipenem. Three-dimensional extract test showed that five were positive for MBLs. The PCR results showed that four were positive for blaVIM， one was positive for blaGIM， and one was positive both for blaVIM and blaGIM. Conclusions　MBLs production was the majior mechanism for MRPA resistant to imipenen in our hospial and the genes of MBLs is of diversity.

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铜绿假单胞菌多重耐药机制中金属β-内酰胺酶(MBLs)耐药基因研究

目的了解我院多药耐药铜绿假单胞菌(MRPA)氨基糖苷修饰酶基因的分布，为临床提供流行病学资料。方法2007 ~ 2009年在我院随机采集铜绿假单胞菌18株。以VITEK-32法为主，部分菌株采用K-B法辅助，对14种抗菌药物进行耐药性鉴定。采用三维提取试验筛选生产MBL的表型。采用聚合酶链反应(PCR)扩增blaIMP、blaVIM、blaSPM、blaGIM和blaSIM 5个基因。结果18株PA中9株对亚胺培南耐药。三维提取试验显示，5株MBLs阳性。PCR结果显示，blaVIM阳性4株，blaGIM阳性1株，blaVIM和blaGIM均阳性1株。结论MBLs的产生是本院MRPA对亚胺培南耐药的主要机制，且MBLs基因具有多样性。

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中华临床医师杂志(电子版)

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