中华临床医师杂志(电子版)最新文献_第2页

The effect of thyroid hormone on heme oxygenase-1 expression after liver ischemia repefusion injury in rat 甲状腺激素对大鼠肝脏缺血再损伤后血红素氧化酶-1表达的影响

中华临床医师杂志(电子版) Pub Date : 2012-11-02 DOI: 10.3877/826

Chen Qi, Wang Meng, Jiang Wei-wei, Kong Lian-bao

{"title":"The effect of thyroid hormone on heme oxygenase-1 expression after liver ischemia repefusion injury in rat","authors":"Chen Qi, Wang Meng, Jiang Wei-wei, Kong Lian-bao","doi":"10.3877/826","DOIUrl":"https://doi.org/10.3877/826","url":null,"abstract":"Objective To explore the effect of thyroid hormone on heme oxygenase-1(HO-1)expression after liver \u0000 \u0000　　ischemia reperfusion injury in rat.Methods An ischemia-reperfusion(I-R)process of 1 h ischemia and then 6 h reperfusion \u0000 \u0000　　was established in male Sprague-Dawley rat.The 30 rats were randomly divided into three groups;sham- \u0000 \u0000　　operated,control,treatment.Treatment of 01 mg/kg intraperitoneal injection of T3 at 48 h before ischemic.The levels of \u0000 \u0000　　ALT,AST in serum and malondialdehyde(MDA)and superoxide dismutase(SOD)in liver tissue were detected at reperfusion for 6 \u0000 \u0000　　hours.The expression of HO-1 were examined by Western blot and RT-PCR.Results Compared to the control group，the \u0000 \u0000　　expression of the HO-1mRNA and protein was higher in the treatment group.The level of ALT,AST in serum samples and MDA in \u0000 \u0000　　liver of the treatment group were significantly lower than that of the control group(P<005)and the level of SOD was higher \u0000 \u0000　　(P<005).Histology showed an severe necrosis in the control group.The necrosis was ameliorated in the treatment \u0000 \u0000　　group.Conclusions These results indicate that thyroid hormone up-regulate the expression of HO-1 and decrease the liver \u0000 \u0000　　ischemic reperfusion injury.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"6 1","pages":"17-20"},"PeriodicalIF":0.0,"publicationDate":"2012-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70279732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical and pathological analysis of hepatosplenic T-cell lymphoma and review of the literature 肝脾t细胞淋巴瘤临床病理分析及文献复习

中华临床医师杂志(电子版) Pub Date : 2012-11-02 DOI: 10.3877/866

Zhang Xiu-ru, Chen Gui-qiu, Tong Chun-rong, Lu Yi-yan

{"title":"Clinical and pathological analysis of hepatosplenic T-cell lymphoma and review of the literature","authors":"Zhang Xiu-ru, Chen Gui-qiu, Tong Chun-rong, Lu Yi-yan","doi":"10.3877/866","DOIUrl":"https://doi.org/10.3877/866","url":null,"abstract":"Objective To study the clinical pathological features of hepatosplenic T-cell lymphoma(HSTCL).Methods In this article we report the clinical material and pathology dignosis of one case of hepatosplenic T-cell lymphoma and review the literature.Results The femal patient was hospitalized with significantly increased size of liver and spleen,accompanied by recurrent fever,pancytopenia.Spleen imprint showed:85% of the cells were naive lymphocytes.Pathology showed:spleen structure was damaged,tumor cells showed sinus infiltration.Tumor cell immune phenotype:CD3 +++,LCA +,PAX5 normal B lymphocyte +,CD68 partial +,CD34 blood vessels +,Ki-67 +>70%,Bcl-2 ++,P53-.EBV-DNA +,quantitative 4×103 copies/106 PBNC.By FC the diagnostic HSTCL immunophenotype was CD4-,CD8-,CD45RA-,CD5-，but CD7+,CD3+,CD2+,TCRγδ+,HLA-DR+,CD38+.Gene rearrangement result showed that:IgH-,TCRγ+.Chromosome karyotype analysis showed:46,X,-X,i(7q),+8[20]/46,XX[3].Conclusions Hepatosplenic T-cell lymphoma is a relatively rare peripheral T-cell lymphoma(peripheral T cell lymphoma,PTCL),the prognosis is poor.Variety of molecular biology-related examinations can be used to assist the diagnosis and differential diagnosis.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"6 1","pages":"394-397"},"PeriodicalIF":0.0,"publicationDate":"2012-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70279931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Value of Kolmogorov entropy on the prediction of seizure in epileptic rats 柯尔莫哥洛夫熵在癫痫大鼠癫痫发作预测中的价值

中华临床医师杂志(电子版) Pub Date : 2012-07-01 DOI: 10.3877/812

Wang Shun-xian, M. Ying, Wang Yin-xu

{"title":"Value of Kolmogorov entropy on the prediction of seizure in epileptic rats","authors":"Wang Shun-xian, M. Ying, Wang Yin-xu","doi":"10.3877/812","DOIUrl":"https://doi.org/10.3877/812","url":null,"abstract":"Objective To investigate the application of Kolmogorov entropy(KE) in epileptic rats induced by lithium-pilocarpine，and to explore its value in predicting epileptic seizures.Methods Twenty-four Sprague-Dawley(SD) rats were randomly divided into 3 groups：a normal group，a control group and epileptic group.Acute chemical models were made by lithium-pilocarpine.The rats were received continuous electroencephalographic(EEG) monitoring by scalp surface electrode.Changes of KE of EEG signal in the epileptic group were analysis in the whole seizure process and compared with those in the normal group and control group.Results In the whole seizure process of the rats induced by lithium-pilocarpine，KE began to decline during the pre-ictal period and dropped sharply during the ictal period.KE in the pre-ictal and ictal period declined apparently comparing with that in the non-ictal period.KE in the epileptic group had significant differences during the ictal and pre-ictal period compared with that in the normal group and the control group(P<0.05).Conclusions Changes of KE reveal the changes of the complex ictal EEG signals，and may be useful to predict epileptic seizure.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"5 1","pages":"7261-7264"},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70279131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of hyperandrogen on the proliferation and apoptosis of ovarian granulosa cells of pre-adolescent mouse 高雄激素对青春期前小鼠卵巢颗粒细胞增殖和凋亡的影响

中华临床医师杂志(电子版) Pub Date : 2012-03-29 DOI: 10.3877/920

Jiang Fan, L. Mei, Zhao Kao-kao, D. Fei-yang, M. Xiang, Cui Yugui, L. Jiayin

{"title":"Effect of hyperandrogen on the proliferation and apoptosis of ovarian granulosa cells of pre-adolescent mouse","authors":"Jiang Fan, L. Mei, Zhao Kao-kao, D. Fei-yang, M. Xiang, Cui Yugui, L. Jiayin","doi":"10.3877/920","DOIUrl":"https://doi.org/10.3877/920","url":null,"abstract":"Objective To observe the effect of testosterone on the proliferation and apoptosis of ovarian granulose cells(GCs)of pre-adolescent mouse,so as to investigate the pathophysiological mechanism of adolescent polycystic ovary syndrome(PCOS).Methods GCs from mouse pre-adolescent ovary were differently treated with testosterone at higher concentration of 10-5,10-6,10-8 mol/L for 24,48,72 h.Cell viability and growth rate of GCs were assayed by MTT methods.After exposure to testosterone(10-5 mol/L)for 48 h,cell cycle and apoptosis rate of GCs were detected by flow cytometry.Caspase-3 activity was detected by absorption spectrometry.Expressions of Caspase-3/9,Bcl-2 and Bax mRNAs in the treated GCs were determined by real-time RT-RCR.Results Cell viability of pre-adolescent mouse GCs was significantly increased in the group treated with testosterone at 10-5 mol/L(P<005).When compared with the control group,the cell cycle from G1 phase into S phase in the testosterone exposure group significantly changed,while the early apoptosis rate significantly decreased by 136%(P<005).Caspase-3 activity significantly decreased in the testosterone treated group,and the expression levels of Caspase-3/9,Bcl-2 and Bax mRNAs significantly decreased(P<005).Conclusions Androgen at higher level can promote cell viability and inhibit the early apoptosis of GCs by inhibiting the mitochondrial pathway of apoptosis-related factor expression.This study suggests that hyperandrogenism in pre-adolescent ovary may be part of pathophysiological mechanism of adolescent PCOS.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"6 1","pages":"1176-1180"},"PeriodicalIF":0.0,"publicationDate":"2012-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70280024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effect of FH535 on proliferation and cyclin D expression in HepG2 cell line FH535对HepG2细胞株增殖及细胞周期蛋白D表达的抑制作用

中华临床医师杂志(电子版) Pub Date : 2012-03-29 DOI: 10.3877/933

Li Guang-bing, Yang Huayu, Mao Yilei, S. Qian, Zhong Shou-xian, Huang Jie-fu

引用次数: 0

The anti-tumor effect of cyanidin-3-glucoside on lung cancer in vitro and in vivo 花青素-3-葡萄糖苷对肺癌的体内外抗肿瘤作用

中华临床医师杂志(电子版) Pub Date : 2012-03-29 DOI: 10.3877/916

Lu Li, Han Lu

{"title":"The anti-tumor effect of cyanidin-3-glucoside on lung cancer in vitro and in vivo","authors":"Lu Li, Han Lu","doi":"10.3877/916","DOIUrl":"https://doi.org/10.3877/916","url":null,"abstract":"Objective To investigate the effect and the mechanism of cyanidin-3-glucoside in the growth inhibition of lung cancer.Methods After human lung cancer cell line H1299 was treated with cyanidin-3-glucoside,the cellular proliferation was detected by methyl thiazolyl tetrazolium(MTT)assay.H1299 cells morphological changes were observed under the fluorescence microscopy after DAPI staining.Western blot was used to detect the expression of XIAP and Smac in lung cancer cells.H1299 cells were injected subcutaneously into nude mice to establish xenograft model.After 2 weeks of implantation,mice were randomized into 2 groups(n=8):Control group,feed with 0.2 ml double distilled water;Test group,feed with cyanidin-3-glucoside at dose of 5 mg/kg.All treatment lasted for three weeks,thrice per week.Eight weeks after implantation,tumor weight and inhibition rate were evaluated respectively after the mice were sacrificed.Immunohistochemistry was used to detect the positive expression of Ki-67,XIAP and Smac in the tumors.Results The proliferation of lung cancer cells was inhibited significantly by cyanidin-3-glucoside with IC50 being 14.5 μg/ml.Cyanidin-3-glucoside induced a higher percentage of apoptosis in H1299 cells when compared with the control.The expression of XIAP and Smac were down-regulated and up-regulated,respectively in H1299 cells after treatment of cyanidin-3-glucoside.The final tumor weight showed significant decrease in the test group compared with control.Furthermore,the positive expression of Ki-67 and XIAP were decreased in tumors after treatment of cyanidin-3-glucoside,but the level of Smac was increased in test group compared with control.Conclusions Cyanidin-3-glucoside exerts anti-tumor activity in lung cancer both in vitro and in vivo,which may be related to down-regulation of XIAP and up-regulation of Smac protein.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"6 1","pages":"1157-1161"},"PeriodicalIF":0.0,"publicationDate":"2012-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70279969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of hemodialysis combined hemofiltration on the level of serum fibroblast growth factor 23 in uremic patients 血液透析联合血液过滤对尿毒症患者血清成纤维细胞生长因子23水平的影响

中华临床医师杂志(电子版) Pub Date : 2012-02-26 DOI: 10.3877/901

Liang Zhu-hong, Li Kai-long, Yang Ju-rong, Zhan Wei-wei, L. Kai-bin, Yang Jie, He Yani

{"title":"Effect of hemodialysis combined hemofiltration on the level of serum fibroblast growth factor 23 in uremic patients","authors":"Liang Zhu-hong, Li Kai-long, Yang Ju-rong, Zhan Wei-wei, L. Kai-bin, Yang Jie, He Yani","doi":"10.3877/901","DOIUrl":"https://doi.org/10.3877/901","url":null,"abstract":"Objective To compare the clear effect of serum FGF23 between traditional hemodialysis(HD)and hemodialysis combined hemofiltration(HF)in uremic patients.Methods A total of 212 patients with chronic kidney disease(CKD)at stage 5 treated with maintenance HD were enrolled in the study,and the study last for 8 weeks.Patients were divided into HD group and HD combined HF group randomly.Prescription for HD group:HD,3 times/week,4 h/time.Prescription for HD combined HF group:HF,1 time/week,6 h/time.HD,2 times/week,4 h/time.Serum phosphorus(P),calcium(Ca),urea nitrogen(BUN),creatinine(Scr),intact parathyroid hormone(iPTH),FGF23 and 1,25(OH)2D3 levels were determined before and after the study.Results There were no significantly difference of P,Ca,BUN,Scr,iPTH,FGF23 and 1,25(OH)2D3 between HD group and HD combined HF group at the beginning of the study(P0.05).No distinctly difference of P,Ca,iPTH,FGF23 and 1,25(OH)2D3 was found in HD group at the end of the study compared with those at the beginning of the study(P0.05),but BUN and Scr decreased obviously(P0.001).In HD combined HF group,no significantly difference of Ca and 1,25(OH)2D3 was found at the end of the study compared with those at the beginning of the study(P0.05),but BUN,Scr,P,iPTH and FGF23 decreased distinctly(P0.001).At the end of the study,P,iPTH and FGF23 decreased significantly in HD combined HF group compared with those in HD group(P0.001).Conclusions Compared with HD,HD combined HF treatment modalities can significantly reduce the phosphorus,iPTH and FGF23 levels in circulating blood of patients with end-stage uremia,indicating that HD combined HF treatment model has a good effect to remove the macromolecule substance,HD combined HF is an ideal combination of treatment modalities.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"6 1","pages":"897-900"},"PeriodicalIF":0.0,"publicationDate":"2012-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70279749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of different types of hypertension on arteriosclerosis in diabetic patients 不同类型高血压对糖尿病患者动脉硬化的影响

中华临床医师杂志(电子版) Pub Date : 2012-02-21 DOI: 10.3877/883

Lv Lin, Y. Pei, S. Yue, Chen Rui, Ma Ze-jun, Dou Hong-mei, Yu De-min

{"title":"Impact of different types of hypertension on arteriosclerosis in diabetic patients","authors":"Lv Lin, Y. Pei, S. Yue, Chen Rui, Ma Ze-jun, Dou Hong-mei, Yu De-min","doi":"10.3877/883","DOIUrl":"https://doi.org/10.3877/883","url":null,"abstract":"Objective To investigate the impact of different types of hypertension on arteriosclerosis in type 2 diabetic patients using a non-invasive vascular screening device.Methods 173 diabetic patients were assigned to 3 groups：group A(without hypertension,n=55),group B(systolic hypertension,n=60)and group C(both systolic and diastolic hypertension,n=58).Arterial stiffness was measured by brachial-ankle pulse wave velocity(baPWV)and ankle-brachial index(ABI).Body mass index(BMI),Glycosylated hemoglobin(HbA1c),high-sensitivity C-reactive protein(hs-CRP),plasma lipid levels were measured.Results baPWV in group B and group C was significantly higher than that in group A(P<0.05),while ABI in group B and group C was lower than that in group A(P<0.05).baPWV in group B was highest among 3 groups(P<0.05),while ABI in group B was lowest(P<0.05).Pulse pressure in group B was highest among 3 groups(P<0.05).Correlation analysis after adjustment for age and sex showed that course of hypertension,course of diabetes,LDL-C,HbA1c,pulse pressure and hs-CRP were significantly related to baPWV(P<0.05 for all).A stepwise multiple regressive analysis demonstrated that age,LDL-C,HbA1c,pulse pressure and hs-CRP were predisposed factors for higher baPWV(P<0.05 for all).Logistic regressive analysis displayed that HbA1c and hs-CRP were risk factors for lower ABI(P<0.05 for all).Conclusions Hypertension aggravates arteriosclerosis in type 2 diabetic patients.Reasonable and long-term administration of hyperglycaemia,hypertension and hyperlipemia would contribute to ameliorate arteriosclerosis in type 2 diabetic patients.","PeriodicalId":63159,"journal":{"name":"中华临床医师杂志(电子版)","volume":"6 1","pages":"631-634"},"PeriodicalIF":0.0,"publicationDate":"2012-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70279688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Male nodular fasciitis of the breast：one case report and literature review 男性乳腺结节性筋膜炎1例报告并文献复习

中华临床医师杂志(电子版) Pub Date : 2012-02-21 DOI: 10.3877/877

Wang Zheng

引用次数: 0

PD98059 enhancing the effects of oxaliplatin on human colorectal cancer RKO cells mediated by downregulation of GST-π expression PD98059通过下调GST-π表达增强奥沙利铂对人大肠癌RKO细胞的作用

中华临床医师杂志(电子版) Pub Date : 2011-12-29 DOI: 10.3877/791

Z. Ye, Q. Xiujuan, Liu Yun-peng, Hou Ke-zuo

引用次数: 1