The effect of thyroid hormone on heme oxygenase-1 expression after liver ischemia repefusion injury in rat

中华临床医师杂志(电子版) Pub Date : 2012-11-02 DOI:10.3877/826

Chen Qi, Wang Meng, Jiang Wei-wei, Kong Lian-bao

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Abstract

Objective To explore the effect of thyroid hormone on heme oxygenase-1(HO-1)expression after liver 　　ischemia reperfusion injury in rat.Methods An ischemia-reperfusion(I-R)process of 1 h ischemia and then 6 h reperfusion 　　was established in male Sprague-Dawley rat.The 30 rats were randomly divided into three groups;sham- 　　operated,control,treatment.Treatment of 01 mg/kg intraperitoneal injection of T3 at 48 h before ischemic.The levels of 　　ALT,AST in serum and malondialdehyde(MDA)and superoxide dismutase(SOD)in liver tissue were detected at reperfusion for 6 　　hours.The expression of HO-1 were examined by Western blot and RT-PCR.Results Compared to the control group，the 　　expression of the HO-1mRNA and protein was higher in the treatment group.The level of ALT,AST in serum samples and MDA in 　　liver of the treatment group were significantly lower than that of the control group(P<005)and the level of SOD was higher 　　(P<005).Histology showed an severe necrosis in the control group.The necrosis was ameliorated in the treatment 　　group.Conclusions These results indicate that thyroid hormone up-regulate the expression of HO-1 and decrease the liver 　　ischemic reperfusion injury.

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甲状腺激素对大鼠肝脏缺血再损伤后血红素氧化酶-1表达的影响

目的探讨甲状腺激素对大鼠肝脏缺血再灌注损伤后血红素加氧酶-1(HO-1)表达的影响。方法建立雄性Sprague-Dawley大鼠缺血1 h再灌注6 h的缺血-再灌注(I-R)过程。将30只大鼠随机分为三组:假手术组、对照组、实验组。缺血前48 h 0 - 1 mg/kg腹腔注射T3治疗。再灌注6 h时检测血清ALT、AST及肝组织丙二醛(MDA)、超氧化物歧化酶(SOD)水平。Western blot和RT-PCR检测HO-1的表达。结果治疗组HO-1mRNA及蛋白表达均高于对照组。治疗组大鼠血清中ALT、AST水平及肝脏中MDA水平均显著低于对照组(P<0, 0.05)， SOD水平显著高于对照组(P<0, 0.05)。组织学显示对照组有严重的坏死。治疗组坏死明显改善。结论甲状腺激素可上调HO-1的表达，减轻肝脏缺血再灌注损伤。

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中华临床医师杂志(电子版)

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